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The map label for this gene is trmB [H]

Identifier: 93006229

GI number: 93006229

Start: 1723682

End: 1724347

Strand: Reverse

Name: trmB [H]

Synonym: Pcryo_1403

Alternate gene names: 93006229

Gene position: 1724347-1723682 (Counterclockwise)

Preceding gene: 93006232

Following gene: 93006226

Centisome position: 56.35

GC content: 42.34

Gene sequence:

>666_bases
ATGACTGACAACCTTGAGCTTTCACATCAGCAAGAGCGGGCATTTCAACCTCAACGACTCTCAGCACCACGTGATTTTTT
GATGCCAGACGCAGCACTTAAACCTAAATCATCACTCGTTTTGGAGATTGGCGCAGGGAAGGGCAAGCATGCCTTAAGCT
TTGCCATGCAAAATCCTGATAAGCATCTGATTGCGATTGAGCGTACTCGCAATAAGTTTGAGGCATTTAAAAAATTGGCA
GCGCAGCAGAATTCTGCCAATTTGACCGCTATTCATGCTGATGCAATTGCATGGATCGTACATGCAATTGCGCCAAACAG
TATCGATAGTATCTATATCTTATATCCCAATCCTGAACAGCACAATCCCAATCAACAATGGCTTAATATGCCGTTTTTTG
AGTTTTTACTGTCACGCTTACAGGTAGGCGGGGAGATAATATTGGCAACCAACATCGAAACATATATGGACAATGCTGAG
CATCAAGCAAATGAAGTATGGTGTTTGCCCAATACGCGTAGTCAGGTTGCAAGCAGTAGTCAGCGTACACATTTTGAAGT
TAAATACTTAGCACGGCAAGAAACCTGTTGGGAATTGAACATGAAGAAACCTGAAGGTTATCAAACCAGATTCGATAAGT
GGATGATAGCTGATGTGAGTATTTAA

Upstream 100 bases:

>100_bases
GATGAGTAATCGATATCGCGATATTACTATTGGGTTGCATTGACTATATAATAGCATGCAGCCGAATAATGACAAATGAC
AAATACTTTAAGGACAGCAA

Downstream 100 bases:

>100_bases
GATAATAGCCTAAGTAAAAGATAAAAGAATTACATTTGCTATAAAATGCTAAGGTTAAGTTAAAACTATAAAATAGCCTA
AGCAATTAAGATTGTAATAA

Product: putative methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MTDNLELSHQQERAFQPQRLSAPRDFLMPDAALKPKSSLVLEIGAGKGKHALSFAMQNPDKHLIAIERTRNKFEAFKKLA
AQQNSANLTAIHADAIAWIVHAIAPNSIDSIYILYPNPEQHNPNQQWLNMPFFEFLLSRLQVGGEIILATNIETYMDNAE
HQANEVWCLPNTRSQVASSSQRTHFEVKYLARQETCWELNMKKPEGYQTRFDKWMIADVSI

Sequences:

>Translated_221_residues
MTDNLELSHQQERAFQPQRLSAPRDFLMPDAALKPKSSLVLEIGAGKGKHALSFAMQNPDKHLIAIERTRNKFEAFKKLA
AQQNSANLTAIHADAIAWIVHAIAPNSIDSIYILYPNPEQHNPNQQWLNMPFFEFLLSRLQVGGEIILATNIETYMDNAE
HQANEVWCLPNTRSQVASSSQRTHFEVKYLARQETCWELNMKKPEGYQTRFDKWMIADVSI
>Mature_220_residues
TDNLELSHQQERAFQPQRLSAPRDFLMPDAALKPKSSLVLEIGAGKGKHALSFAMQNPDKHLIAIERTRNKFEAFKKLAA
QQNSANLTAIHADAIAWIVHAIAPNSIDSIYILYPNPEQHNPNQQWLNMPFFEFLLSRLQVGGEIILATNIETYMDNAEH
QANEVWCLPNTRSQVASSSQRTHFEVKYLARQETCWELNMKKPEGYQTRFDKWMIADVSI

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 25290; Mature: 25159

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNLELSHQQERAFQPQRLSAPRDFLMPDAALKPKSSLVLEIGAGKGKHALSFAMQNPD
CCCCCCCCCHHHHCCCCHHCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCC
KHLIAIERTRNKFEAFKKLAAQQNSANLTAIHADAIAWIVHAIAPNSIDSIYILYPNPEQ
CEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCC
HNPNQQWLNMPFFEFLLSRLQVGGEIILATNIETYMDNAEHQANEVWCLPNTRSQVASSS
CCCCHHHCCCCHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCEEEECCCCHHHHHCCC
QRTHFEVKYLARQETCWELNMKKPEGYQTRFDKWMIADVSI
CCCEEEEEEEHHCCCEEEECCCCCCCCHHHCCEEEEEEECC
>Mature Secondary Structure 
TDNLELSHQQERAFQPQRLSAPRDFLMPDAALKPKSSLVLEIGAGKGKHALSFAMQNPD
CCCCCCCCHHHHCCCCHHCCCCHHHCCCCCCCCCCCCEEEEECCCCCCCEEEEEECCCC
KHLIAIERTRNKFEAFKKLAAQQNSANLTAIHADAIAWIVHAIAPNSIDSIYILYPNPEQ
CEEEEEEHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCC
HNPNQQWLNMPFFEFLLSRLQVGGEIILATNIETYMDNAEHQANEVWCLPNTRSQVASSS
CCCCHHHCCCCHHHHHHHHHHCCCEEEEEECHHHHHCCCCCCCCEEEECCCCHHHHHCCC
QRTHFEVKYLARQETCWELNMKKPEGYQTRFDKWMIADVSI
CCCEEEEEEEHHCCCEEEECCCCCCCCHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA