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The map label for this gene is nemA [H]

Identifier: 93006154

GI number: 93006154

Start: 1623201

End: 1624445

Strand: Direct

Name: nemA [H]

Synonym: Pcryo_1326

Alternate gene names: 93006154

Gene position: 1623201-1624445 (Clockwise)

Preceding gene: 93006152

Following gene: 93006156

Centisome position: 53.05

GC content: 46.27

Gene sequence:

>1245_bases
ATGCTGACCAACATTGCTCAAGTAATCATTAGATTGGTTATTAAGCATCAAAACAGACCAAATACATTTAATAATAAGCC
ATCAGCTGCTTTAAAAGAGACATTTATGACACAAACCATTCTAAATACCAGTACACTATTTTCATCTGTACAACTTGGAC
CTTATACCCTTAAAAACCGTATCGTGATGCCGCCTTTGACGCGCTCTCGTAGCACGCAACCTGATAATATCCCTAATGAT
TTAATGGCAAGCTATTATGCACAGCGCGCCTCAGCGGGCTTTATGGTGACTGAAGGTACGCAGATTGAACCAAGAGGCCA
AGGTTATGCATGGACGCCCGGGATTCATTCACCAGCCCAAGTTGAAGGCTGGAAAAAAGTCACGCAGGCAGTACACGCTA
AAGGTAGTATTATTTTTGCGCAGCTGTGGCACGTTGGCCGCGTGTCTCATACCAGCTTACAGCCGCAAGGTGCTCAGCCT
GTAGGCCCATCAGCGATTGCTGCTGACAATGTAAAAGTATTTATTGAAACAGGCCCAGGTACAGGCGCATTGGCCGATCC
AAGTGAGCCACGCGCACTGAGCACAGAAGAGGTGGGTGAGCTGGTCGCTATGTATGCGGCAGCCGCACGCAACGCATTAG
AAGCAGGTTTTGATGGGGTAGAGCTACACTGTGCCAATGGCTATTTGGTCAATCAATTTATCTCTGAGCATAGCAATACA
CGTGATGATCAATATGGTGGTTCGTTGACCAATCGTCTGCGTTTTTTAAAAGAAGTAGTCGCTGCCGTCAGTGAGGTGGT
TGGCGCTGATCGTTTAGGCGTACGTTTTGCGCCTTTATTTGCCAGCACGGATGAGACTCGTGTGTATCTAGGGCTAGTAG
AAGCGGACCCACATCATACCTATATTGAAGCGATTAAGGTGCTTGAGCAGGCTGGTATTGCTTACTTGTCCATTGCAGAG
GCTGATTGGGATAATGCGCCTGATTTGCCAGAGCCTTTTTACCAAGCCGTCAGAGCTGAGTTTTCAGGACGTATTATTTA
TGCGGGTAAGTATACTGTGCAGAAGTCGGTCGATATATTATCTAAAGGTTATGGGGATTTATTTGCTTTTGGTCGTCCTT
TTATTGCTAATCCCGATTTACCTGAACGCATAGCCAATGATTGGCCACTCAATGAGGCAGATCCTGTTACGATGTATGGT
GGCACTGCCATTGGCTACAGTGATTATCCTCGCTATCAGGAATAG

Upstream 100 bases:

>100_bases
TGCTTGATCTCTAGCCGGTTCATACCATTCATAAAACTCACCATTAATAATTGTACCATAAACAAGAAAGGCTTGTTTCT
AATTGTACCCATAAAACTCT

Downstream 100 bases:

>100_bases
CAAAGCAGCTGAGCAGTGAATGAGATAAGGCCATTAATACTAATGGCCTTATTTTTATTTATAAATTTGAGACTTGTTGG
ATATTCACAAACAGTCAATC

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MLTNIAQVIIRLVIKHQNRPNTFNNKPSAALKETFMTQTILNTSTLFSSVQLGPYTLKNRIVMPPLTRSRSTQPDNIPND
LMASYYAQRASAGFMVTEGTQIEPRGQGYAWTPGIHSPAQVEGWKKVTQAVHAKGSIIFAQLWHVGRVSHTSLQPQGAQP
VGPSAIAADNVKVFIETGPGTGALADPSEPRALSTEEVGELVAMYAAAARNALEAGFDGVELHCANGYLVNQFISEHSNT
RDDQYGGSLTNRLRFLKEVVAAVSEVVGADRLGVRFAPLFASTDETRVYLGLVEADPHHTYIEAIKVLEQAGIAYLSIAE
ADWDNAPDLPEPFYQAVRAEFSGRIIYAGKYTVQKSVDILSKGYGDLFAFGRPFIANPDLPERIANDWPLNEADPVTMYG
GTAIGYSDYPRYQE

Sequences:

>Translated_414_residues
MLTNIAQVIIRLVIKHQNRPNTFNNKPSAALKETFMTQTILNTSTLFSSVQLGPYTLKNRIVMPPLTRSRSTQPDNIPND
LMASYYAQRASAGFMVTEGTQIEPRGQGYAWTPGIHSPAQVEGWKKVTQAVHAKGSIIFAQLWHVGRVSHTSLQPQGAQP
VGPSAIAADNVKVFIETGPGTGALADPSEPRALSTEEVGELVAMYAAAARNALEAGFDGVELHCANGYLVNQFISEHSNT
RDDQYGGSLTNRLRFLKEVVAAVSEVVGADRLGVRFAPLFASTDETRVYLGLVEADPHHTYIEAIKVLEQAGIAYLSIAE
ADWDNAPDLPEPFYQAVRAEFSGRIIYAGKYTVQKSVDILSKGYGDLFAFGRPFIANPDLPERIANDWPLNEADPVTMYG
GTAIGYSDYPRYQE
>Mature_414_residues
MLTNIAQVIIRLVIKHQNRPNTFNNKPSAALKETFMTQTILNTSTLFSSVQLGPYTLKNRIVMPPLTRSRSTQPDNIPND
LMASYYAQRASAGFMVTEGTQIEPRGQGYAWTPGIHSPAQVEGWKKVTQAVHAKGSIIFAQLWHVGRVSHTSLQPQGAQP
VGPSAIAADNVKVFIETGPGTGALADPSEPRALSTEEVGELVAMYAAAARNALEAGFDGVELHCANGYLVNQFISEHSNT
RDDQYGGSLTNRLRFLKEVVAAVSEVVGADRLGVRFAPLFASTDETRVYLGLVEADPHHTYIEAIKVLEQAGIAYLSIAE
ADWDNAPDLPEPFYQAVRAEFSGRIIYAGKYTVQKSVDILSKGYGDLFAFGRPFIANPDLPERIANDWPLNEADPVTMYG
GTAIGYSDYPRYQE

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=372, Percent_Identity=47.0430107526882, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1789463, Length=350, Percent_Identity=31.4285714285714, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17559802, Length=359, Percent_Identity=27.0194986072423, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17565138, Length=307, Percent_Identity=27.3615635179153, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17559804, Length=357, Percent_Identity=26.6106442577031, Blast_Score=101, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=356, Percent_Identity=25.8426966292135, Blast_Score=95, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=355, Percent_Identity=25.6338028169014, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=358, Percent_Identity=25.6983240223464, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=355, Percent_Identity=24.2253521126761, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=398, Percent_Identity=35.929648241206, Blast_Score=210, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6325086, Length=404, Percent_Identity=35.6435643564356, Blast_Score=207, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45251; Mature: 45251

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNIAQVIIRLVIKHQNRPNTFNNKPSAALKETFMTQTILNTSTLFSSVQLGPYTLKNR
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCE
IVMPPLTRSRSTQPDNIPNDLMASYYAQRASAGFMVTEGTQIEPRGQGYAWTPGIHSPAQ
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCEECCCCCCEEECCCCCCCHH
VEGWKKVTQAVHAKGSIIFAQLWHVGRVSHTSLQPQGAQPVGPSAIAADNVKVFIETGPG
HHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEEECCC
TGALADPSEPRALSTEEVGELVAMYAAAARNALEAGFDGVELHCANGYLVNQFISEHSNT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCC
RDDQYGGSLTNRLRFLKEVVAAVSEVVGADRLGVRFAPLFASTDETRVYLGLVEADPHHT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCHH
YIEAIKVLEQAGIAYLSIAEADWDNAPDLPEPFYQAVRAEFSGRIIYAGKYTVQKSVDIL
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEECCEEHHHHHHHH
SKGYGDLFAFGRPFIANPDLPERIANDWPLNEADPVTMYGGTAIGYSDYPRYQE
HCCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCEEEECCEECCCCCCCCCCC
>Mature Secondary Structure
MLTNIAQVIIRLVIKHQNRPNTFNNKPSAALKETFMTQTILNTSTLFSSVQLGPYTLKNR
CCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCE
IVMPPLTRSRSTQPDNIPNDLMASYYAQRASAGFMVTEGTQIEPRGQGYAWTPGIHSPAQ
EECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCEECCCCCCEEECCCCCCCHH
VEGWKKVTQAVHAKGSIIFAQLWHVGRVSHTSLQPQGAQPVGPSAIAADNVKVFIETGPG
HHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEEECCC
TGALADPSEPRALSTEEVGELVAMYAAAARNALEAGFDGVELHCANGYLVNQFISEHSNT
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCC
RDDQYGGSLTNRLRFLKEVVAAVSEVVGADRLGVRFAPLFASTDETRVYLGLVEADPHHT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEEECCCCHH
YIEAIKVLEQAGIAYLSIAEADWDNAPDLPEPFYQAVRAEFSGRIIYAGKYTVQKSVDIL
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCEEEEECCEEHHHHHHHH
SKGYGDLFAFGRPFIANPDLPERIANDWPLNEADPVTMYGGTAIGYSDYPRYQE
HCCCHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCCEEEECCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]