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The map label for this gene is benA [H]

Identifier: 93006095

GI number: 93006095

Start: 1537730

End: 1539112

Strand: Reverse

Name: benA [H]

Synonym: Pcryo_1267

Alternate gene names: 93006095

Gene position: 1539112-1537730 (Counterclockwise)

Preceding gene: 93006096

Following gene: 93006094

Centisome position: 50.3

GC content: 44.61

Gene sequence:

>1383_bases
ATGAGCGAGCGTATCAATCTAGTACCTGAAGGATCAGACATCACTATTGATGATTTTTTAGAAGAGAACAGTGAAAAAGG
GATTTATCGCCTGCATCGTAGCGCCTTCACTAACGAAGAGTTGTTCGAGCTTGAGATGAAGTACATCTTTGAAAACAATT
GGGTGTATTTGGCTCATGAGTCTCAAGTTCCTGATAACAACGACTACTACACCTCATACATTGGTCGTCAGCCTGTTATT
CTTGCCCGCAATAAAGAAGGCGAGCTGCATTGCATGATTAACTCATGCTCACATCGTGGTGCACAGCTCTGCCGACATAA
AAAAGGCAATAAAGCCACTTACACCTGCCCATTTCATGGTTGGACCTTTAACAACTCAGGCAAGCTGCTTAAAGTGAAAG
ACCCAAGACATGCAGGCTATCCAGAATGCTTCAATAAAGAAAGCTCACACGACCTAAAACAAGTAAAGATGGCCAACTAT
AAAGGCTTTATTTTTGCTAGCTTAAATGATGATGTCGACCCTATCGAAGACTGGCTTGGTGGTTCAACCAAGATTTTAGA
TATGATTGTCAACCAATCACCAGAAGGCCTAGAAGTGTTACGTGGTACCTCAACCTATACCTTTGATGGTAACTGGAAAC
TACAAGCTGAAAATGGCGCTGATGGCTACCACGTCTCAGCCGTACATTGGAACTATGCTGCCACGACTGCACGCCGTAAA
GAAGAAACCGCTAAAGAAGACAATATTAAAGCCATGAATGCTGGCAAATGGGGTCGTCAAGGTGGCGGCTTCTATTCTTT
TGAAAACGGTCACATGTTGTTATGGACACAATGGGAAAACCCACAAGATCGTCCAAACTATGCCTATCGCGATGACTATA
TCGCCAGCTACGGTGAAGCCATGGCGGACTGGATGATTGGGCGCTCACGTAATTTATGCCTATATCCAAACGTCTACATC
ATGGATCAATTCAGCTCACAAATCCGTATGCTCCGCCCTATCGCGGTTGACAAAACTGAAGTCAGTATCTGGTGTATCGC
ACCAAAAGGCGAAAGCGATGAGGCACGTGCACGCCGTATTCGTCAGTATGAAGATTTCTTTAACGTTACTGGTATGGCAA
CACCTGATGACTTAGAAGAGTTCCGCTCGTGTCAAGAAGGTTATAACGGTATCAAGCTTGAATGGAATGATATGTGCCGA
GGCTCTGAGCATTGGATTCAAGGTGCAGATGAAGGCGCTGCCGCTATTGACCTCAAACCTTTAATGAGTGGTGTTAAGAC
TGAAGATGAAGGACTATATACCGTCCAACATCGCTACTGGCATGAAGAGATGAAAAAAGCTCTAGAGCAAGAGCAGCAGC
TACACGGTGCTAATAGCGACTAA

Upstream 100 bases:

>100_bases
AATAACGATTAGCGACTTACGTTTGATATGTCCTCAGTTGTCGTATCAAACGTTAGCTTCTAATCTCATTTTATACCGTC
ATTATACAAGGAAGGCAGCC

Downstream 100 bases:

>100_bases
TACATTTTTATTATTACTCTGCTATTTATTGTAGAACTGACAAAAAAATAAGGATATCAATCATGAAAAATGCAACTGTT
AACATTGAAGACATTAGACA

Product: Rieske (2Fe-2S) domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MSERINLVPEGSDITIDDFLEENSEKGIYRLHRSAFTNEELFELEMKYIFENNWVYLAHESQVPDNNDYYTSYIGRQPVI
LARNKEGELHCMINSCSHRGAQLCRHKKGNKATYTCPFHGWTFNNSGKLLKVKDPRHAGYPECFNKESSHDLKQVKMANY
KGFIFASLNDDVDPIEDWLGGSTKILDMIVNQSPEGLEVLRGTSTYTFDGNWKLQAENGADGYHVSAVHWNYAATTARRK
EETAKEDNIKAMNAGKWGRQGGGFYSFENGHMLLWTQWENPQDRPNYAYRDDYIASYGEAMADWMIGRSRNLCLYPNVYI
MDQFSSQIRMLRPIAVDKTEVSIWCIAPKGESDEARARRIRQYEDFFNVTGMATPDDLEEFRSCQEGYNGIKLEWNDMCR
GSEHWIQGADEGAAAIDLKPLMSGVKTEDEGLYTVQHRYWHEEMKKALEQEQQLHGANSD

Sequences:

>Translated_460_residues
MSERINLVPEGSDITIDDFLEENSEKGIYRLHRSAFTNEELFELEMKYIFENNWVYLAHESQVPDNNDYYTSYIGRQPVI
LARNKEGELHCMINSCSHRGAQLCRHKKGNKATYTCPFHGWTFNNSGKLLKVKDPRHAGYPECFNKESSHDLKQVKMANY
KGFIFASLNDDVDPIEDWLGGSTKILDMIVNQSPEGLEVLRGTSTYTFDGNWKLQAENGADGYHVSAVHWNYAATTARRK
EETAKEDNIKAMNAGKWGRQGGGFYSFENGHMLLWTQWENPQDRPNYAYRDDYIASYGEAMADWMIGRSRNLCLYPNVYI
MDQFSSQIRMLRPIAVDKTEVSIWCIAPKGESDEARARRIRQYEDFFNVTGMATPDDLEEFRSCQEGYNGIKLEWNDMCR
GSEHWIQGADEGAAAIDLKPLMSGVKTEDEGLYTVQHRYWHEEMKKALEQEQQLHGANSD
>Mature_459_residues
SERINLVPEGSDITIDDFLEENSEKGIYRLHRSAFTNEELFELEMKYIFENNWVYLAHESQVPDNNDYYTSYIGRQPVIL
ARNKEGELHCMINSCSHRGAQLCRHKKGNKATYTCPFHGWTFNNSGKLLKVKDPRHAGYPECFNKESSHDLKQVKMANYK
GFIFASLNDDVDPIEDWLGGSTKILDMIVNQSPEGLEVLRGTSTYTFDGNWKLQAENGADGYHVSAVHWNYAATTARRKE
ETAKEDNIKAMNAGKWGRQGGGFYSFENGHMLLWTQWENPQDRPNYAYRDDYIASYGEAMADWMIGRSRNLCLYPNVYIM
DQFSSQIRMLRPIAVDKTEVSIWCIAPKGESDEARARRIRQYEDFFNVTGMATPDDLEEFRSCQEGYNGIKLEWNDMCRG
SEHWIQGADEGAAAIDLKPLMSGVKTEDEGLYTVQHRYWHEEMKKALEQEQQLHGANSD

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB) [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788888, Length=412, Percent_Identity=27.9126213592233, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788103, Length=227, Percent_Identity=27.3127753303965, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017639
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 53010; Mature: 52878

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00570 RING_HYDROXYL_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERINLVPEGSDITIDDFLEENSEKGIYRLHRSAFTNEELFELEMKYIFENNWVYLAHE
CCCCEEEEECCCCEEHHHHHHCCCCCCHHHHHHHHCCCCHHHHEEEEEEEECCEEEEEEC
SQVPDNNDYYTSYIGRQPVILARNKEGELHCMINSCSHRGAQLCRHKKGNKATYTCPFHG
CCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEECCCC
WTFNNSGKLLKVKDPRHAGYPECFNKESSHDLKQVKMANYKGFIFASLNDDVDPIEDWLG
EEECCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHCC
GSTKILDMIVNQSPEGLEVLRGTSTYTFDGNWKLQAENGADGYHVSAVHWNYAATTARRK
CCHHHHHHHHCCCCCHHHHEECCEEEEECCCEEEEECCCCCCEEEEEEEECHHHHHHHHH
EETAKEDNIKAMNAGKWGRQGGGFYSFENGHMLLWTQWENPQDRPNYAYRDDYIASYGEA
HHHHHHCCCEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHH
MADWMIGRSRNLCLYPNVYIMDQFSSQIRMLRPIAVDKTEVSIWCIAPKGESDEARARRI
HHHHHHCCCCCEEECCCEEEEECHHHHHHHHCCEEECCCEEEEEEEECCCCCHHHHHHHH
RQYEDFFNVTGMATPDDLEEFRSCQEGYNGIKLEWNDMCRGSEHWIQGADEGAAAIDLKP
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCHHHHCCCCCCCEEEEHHH
LMSGVKTEDEGLYTVQHRYWHEEMKKALEQEQQLHGANSD
HHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SERINLVPEGSDITIDDFLEENSEKGIYRLHRSAFTNEELFELEMKYIFENNWVYLAHE
CCCEEEEECCCCEEHHHHHHCCCCCCHHHHHHHHCCCCHHHHEEEEEEEECCEEEEEEC
SQVPDNNDYYTSYIGRQPVILARNKEGELHCMINSCSHRGAQLCRHKKGNKATYTCPFHG
CCCCCCCCEEEEECCCCCEEEEECCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEECCCC
WTFNNSGKLLKVKDPRHAGYPECFNKESSHDLKQVKMANYKGFIFASLNDDVDPIEDWLG
EEECCCCCEEEEECCCCCCCHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHCC
GSTKILDMIVNQSPEGLEVLRGTSTYTFDGNWKLQAENGADGYHVSAVHWNYAATTARRK
CCHHHHHHHHCCCCCHHHHEECCEEEEECCCEEEEECCCCCCEEEEEEEECHHHHHHHHH
EETAKEDNIKAMNAGKWGRQGGGFYSFENGHMLLWTQWENPQDRPNYAYRDDYIASYGEA
HHHHHHCCCEEEECCCCCCCCCCEEEECCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHH
MADWMIGRSRNLCLYPNVYIMDQFSSQIRMLRPIAVDKTEVSIWCIAPKGESDEARARRI
HHHHHHCCCCCEEECCCEEEEECHHHHHHHHCCEEECCCEEEEEEEECCCCCHHHHHHHH
RQYEDFFNVTGMATPDDLEEFRSCQEGYNGIKLEWNDMCRGSEHWIQGADEGAAAIDLKP
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCHHHHCCCCCCCEEEEHHH
LMSGVKTEDEGLYTVQHRYWHEEMKKALEQEQQLHGANSD
HHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]