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The map label for this gene is benB [H]

Identifier: 93006094

GI number: 93006094

Start: 1537170

End: 1537667

Strand: Reverse

Name: benB [H]

Synonym: Pcryo_1266

Alternate gene names: 93006094

Gene position: 1537667-1537170 (Counterclockwise)

Preceding gene: 93006095

Following gene: 93006093

Centisome position: 50.25

GC content: 42.97

Gene sequence:

>498_bases
ATGAAAAATGCAACTGTTAACATTGAAGACATTAGACAGTTTCTATACCGTGAAGCGCGTTTATTGGATGAGAAAAAATG
GGAAGAATGGCTCACTTATTACGATGAAGACTGCCCTTTCTGGATGCCAAGTTGGGATGATGATGGTCAGCTCATCAGCG
ACCCACAACGAGAAATCTCGCTGATTTATTATCCGGATCGCCGTGGTCTTGAAGATCGCATCTTCCGCATCGAGACTGAG
CGCTCATCAGCGACCATACCTGATACGCGTACCACGCACAATTTATCGAATATTGAACTACTCGAAGACAATGACGGTAT
TGTGACGGTGCGTTTCAACTGGATCAACAAGTCCTTTCGCTATCACGAGACCAATACCTATTGGGGTTACTCTCAGTACA
AAATAGACATGACTCAAGACCAACCAAAAATCTTGGATAAGTATATGGTCTTAAAAGACGACTATGTGCATCAAATCATG
GACGTCTATCACGTCTAA

Upstream 100 bases:

>100_bases
AGCAAGAGCAGCAGCTACACGGTGCTAATAGCGACTAATACATTTTTATTATTACTCTGCTATTTATTGTAGAACTGACA
AAAAAATAAGGATATCAATC

Downstream 100 bases:

>100_bases
TCATTATTCACTCATTATTTAGCCACTAGCTTTTAATTAATACGTGTTGAAAAGGGATATTTCTCATGGAATATAATATT
GCTCTACAGTTCGAAGACGG

Product: 2-chlorobenzoate 1,2-dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MKNATVNIEDIRQFLYREARLLDEKKWEEWLTYYDEDCPFWMPSWDDDGQLISDPQREISLIYYPDRRGLEDRIFRIETE
RSSATIPDTRTTHNLSNIELLEDNDGIVTVRFNWINKSFRYHETNTYWGYSQYKIDMTQDQPKILDKYMVLKDDYVHQIM
DVYHV

Sequences:

>Translated_165_residues
MKNATVNIEDIRQFLYREARLLDEKKWEEWLTYYDEDCPFWMPSWDDDGQLISDPQREISLIYYPDRRGLEDRIFRIETE
RSSATIPDTRTTHNLSNIELLEDNDGIVTVRFNWINKSFRYHETNTYWGYSQYKIDMTQDQPKILDKYMVLKDDYVHQIM
DVYHV
>Mature_165_residues
MKNATVNIEDIRQFLYREARLLDEKKWEEWLTYYDEDCPFWMPSWDDDGQLISDPQREISLIYYPDRRGLEDRIFRIETE
RSSATIPDTRTTHNLSNIELLEDNDGIVTVRFNWINKSFRYHETNTYWGYSQYKIDMTQDQPKILDKYMVLKDDYVHQIM
DVYHV

Specific function: Degradation of benzoate to 2-hydro-1,2-dihydroxybenzoate (DHB). The beta subunit may be responsible for the substrate specificity of the enzyme [H]

COG id: COG5517

COG function: function code Q; Small subunit of phenylpropionate dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial ring-hydroxylating dioxygenase beta subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017641
- InterPro:   IPR000391 [H]

Pfam domain/function: PF00866 Ring_hydroxyl_B [H]

EC number: =1.14.12.10 [H]

Molecular weight: Translated: 20181; Mature: 20181

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNATVNIEDIRQFLYREARLLDEKKWEEWLTYYDEDCPFWMPSWDDDGQLISDPQREIS
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCEEE
LIYYPDRRGLEDRIFRIETERSSATIPDTRTTHNLSNIELLEDNDGIVTVRFNWINKSFR
EEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCEE
YHETNTYWGYSQYKIDMTQDQPKILDKYMVLKDDYVHQIMDVYHV
EECCCCEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNATVNIEDIRQFLYREARLLDEKKWEEWLTYYDEDCPFWMPSWDDDGQLISDPQREIS
CCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCCCCEEE
LIYYPDRRGLEDRIFRIETERSSATIPDTRTTHNLSNIELLEDNDGIVTVRFNWINKSFR
EEEECCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCEE
YHETNTYWGYSQYKIDMTQDQPKILDKYMVLKDDYVHQIMDVYHV
EECCCCEECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1885518 [H]