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The map label for this gene is ispF [H]

Identifier: 93005977

GI number: 93005977

Start: 1392778

End: 1393284

Strand: Direct

Name: ispF [H]

Synonym: Pcryo_1149

Alternate gene names: 93005977

Gene position: 1392778-1393284 (Clockwise)

Preceding gene: 93005973

Following gene: 93005978

Centisome position: 45.52

GC content: 44.97

Gene sequence:

>507_bases
ATGATAATGATAAAAATTGGTCAAGGTATTGATGTTCATGCGTTTCATAATAATGGTCAACAGCAGCAATATGTGGTGCT
GGCAGGTGTGCCTATTGATCATACGCACAGCTTACTTGCGCATTCTGATGGTGATGTAGTACTGCATGCACTTGCTGACG
CTTTGCTTGGCGCCTTGGCACTTGGCGATATCGGGCAACATTTTCCAGATACTGATGCGGCAAATGCGGGTTTAGATTCA
CGCGTACTACTTCGTTATGTGTATGGCAAAGTGTTAGCGGCAGGTTATATATTGGGTAATGCTGATATTACCGTAATGTG
TGAGCGTCCAAAACTGGCACCACATAATTTAGCCATGCGCGCTAACATCGCTAGTGATTTGCAGACCGATGTGAGTAATA
TCAGTGTTAAGGCAACCACCACTGAAAAATTGGGCTTTACCGGTCGGCAAGAGGGTATTATGGCCAATGCCGTGGTATTA
TTGGTGCCGAATGCGTCTGGTGTTTAA

Upstream 100 bases:

>100_bases
GAAACTGGTGGCGAGCTAAGGCATCTTACTGGCGGTAAAATCGCTTTAAAAATTTAGATAATGGTTTTCAATTATCCTAA
TCACCATTAATTTACTTGGA

Downstream 100 bases:

>100_bases
TTCTCAATAGTATATCAGTGCGTATGATTATGGCTGTCAGCTTTCAAAACTGCTTGCAATATGGTTAAGATTGCCCCATG
TAGCAGCCACATGATAAATT

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 168; Mature: 168

Protein sequence:

>168_residues
MIMIKIGQGIDVHAFHNNGQQQQYVVLAGVPIDHTHSLLAHSDGDVVLHALADALLGALALGDIGQHFPDTDAANAGLDS
RVLLRYVYGKVLAAGYILGNADITVMCERPKLAPHNLAMRANIASDLQTDVSNISVKATTTEKLGFTGRQEGIMANAVVL
LVPNASGV

Sequences:

>Translated_168_residues
MIMIKIGQGIDVHAFHNNGQQQQYVVLAGVPIDHTHSLLAHSDGDVVLHALADALLGALALGDIGQHFPDTDAANAGLDS
RVLLRYVYGKVLAAGYILGNADITVMCERPKLAPHNLAMRANIASDLQTDVSNISVKATTTEKLGFTGRQEGIMANAVVL
LVPNASGV
>Mature_168_residues
MIMIKIGQGIDVHAFHNNGQQQQYVVLAGVPIDHTHSLLAHSDGDVVLHALADALLGALALGDIGQHFPDTDAANAGLDS
RVLLRYVYGKVLAAGYILGNADITVMCERPKLAPHNLAMRANIASDLQTDVSNISVKATTTEKLGFTGRQEGIMANAVVL
LVPNASGV

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=159, Percent_Identity=55.3459119496855, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 17686; Mature: 17686

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIMIKIGQGIDVHAFHNNGQQQQYVVLAGVPIDHTHSLLAHSDGDVVLHALADALLGALA
CEEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHH
LGDIGQHFPDTDAANAGLDSRVLLRYVYGKVLAAGYILGNADITVMCERPKLAPHNLAMR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCEEEE
ANIASDLQTDVSNISVKATTTEKLGFTGRQEGIMANAVVLLVPNASGV
CCCHHHHHHHHHHEEEEEECHHHCCCCCCCCCEEEEEEEEEECCCCCC
>Mature Secondary Structure
MIMIKIGQGIDVHAFHNNGQQQQYVVLAGVPIDHTHSLLAHSDGDVVLHALADALLGALA
CEEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHH
LGDIGQHFPDTDAANAGLDSRVLLRYVYGKVLAAGYILGNADITVMCERPKLAPHNLAMR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCEEEEECCCCCCCCCEEEE
ANIASDLQTDVSNISVKATTTEKLGFTGRQEGIMANAVVLLVPNASGV
CCCHHHHHHHHHHEEEEEECHHHCCCCCCCCCEEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA