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The map label for this gene is purB [H]

Identifier: 93005974

GI number: 93005974

Start: 1387932

End: 1389323

Strand: Reverse

Name: purB [H]

Synonym: Pcryo_1146

Alternate gene names: 93005974

Gene position: 1389323-1387932 (Counterclockwise)

Preceding gene: 93005975

Following gene: 93005972

Centisome position: 45.4

GC content: 42.39

Gene sequence:

>1392_bases
ATGAATCTACTTACCGCACTCTCCCCAATCGATGGTCGCTATGCATCCAAAGCCGATAGCTTGCGCCCTTATTTATCAGA
ATTTGGTCTGATTAAAGCGCGTGTGACCGTTGAGATTCGCTGGTTACAGTCATTGGCTGATAATAGCGCGATTGGTGAGT
TGGCAGAATTTGATACCAATACCAATGCGTTTTTAAATGCTATCGTAGAGAACTTTAGCGAAGCTGACGCGCAAGCGATC
AAAGACATCGAAGCAACGACCAACCATGATGTAAAAGCGGTTGAATACTTTATTAAAGATAAATTCCGTGGTCAGGCAGC
ACTTGAAGATTCATTAGAATTTATTCATTTTGCTTGTACCAGTGAAGATATTAATAACCTATCTTACGCACTCATGCTCA
AAGACAGCCGCGAAATCGTCGTTGCTAAAATGCAAGAAGTAACGGATAGCATCGTTAATCTAGCAATTATCCATGCTGAT
CAACCGATGCTTTCACGTACTCATGGTCAAACGGCAAGCCCGACGACTCTAGGTAAAGAGATGGCAAACGTTGCTTATCG
CCTTGCTCGTCAAATTAAGCAAGTCGGTCAGGTAGAGTTACTCGGTAAAATCAATGGTGCGGTCGGTAACTATAATGCTC
ACTACGCCTCATATCCTGACGTTGATTGGCAGAATCATGCTGAAAAATTCATTAATGAACGTTTAGAATTGACCTTTAAT
CCTTATACCACGCAAATTGAGCCGCATGATTATATCGCTGAATTGTTTGACGCAGTAAAACGCTTTAATACCATTCTAAT
CGACTTTAACCGTGATATTTGGCAGTATATTAGCTTAGGCTATTTTAAACAGCGCCTAAAAGATGGTGAAGTGGGTTCTT
CTACCATGCCGCATAAAGTCAACCCGATTGACTTTGAAAACTCTGAAGGTAATTTGGGTGTAGCTAATGCCATGCTTGCT
CATTTGGGTGAAAAGCTACCGATTTCACGTATGCAGCGCGATTTGTCTGACTCTACCGTGTTGCGTAATATCGGTGTTGG
TCTAGCACAAAGCATGATTGCTTATGATGCGTGCCTCAAAGGCGTCAGCAAACTTGAATTAAATGCCCAACGTTTAAATG
ATGACCTAGATAATGCGCAAGAAGTGTTGGCAGAGCCGATTCAAACGGTCATGCGTCGCTACCGTGTTGAAAACCCATAC
GAGAAGCTGAAAGCTCTTACCCGTGGTAATGCCATGACTCGTGAAGCAATGCTTACTTTTGTTGATGGCGATGAATTATC
GGCAGTCAGTGAAAGCGATAAAAACAGTTTGCGCGAAATGACGCCTGCTACTTATATTGGTAATGCGGCTGAACAAGCAC
GCACGATTAAAGAATGGATTGCTAAAATTTAA

Upstream 100 bases:

>100_bases
TACAACGCAATCTAATTAATACATGATGATTTTTCAGATTAAATCATTTTTTACATTTTTTTAACCAGCTGTAATTTCAA
TAACTTTTTGAGGCAATGAT

Downstream 100 bases:

>100_bases
CTGGCAATCAGCTCATCTCTATCAAATGCAAAAAAGATATAAATGAGCACTCATTTATATCTTAATAAGAACCTTACTCT
AAACACTATTATTTTTTAAA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNLLTALSPIDGRYASKADSLRPYLSEFGLIKARVTVEIRWLQSLADNSAIGELAEFDTNTNAFLNAIVENFSEADAQAI
KDIEATTNHDVKAVEYFIKDKFRGQAALEDSLEFIHFACTSEDINNLSYALMLKDSREIVVAKMQEVTDSIVNLAIIHAD
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQVGQVELLGKINGAVGNYNAHYASYPDVDWQNHAEKFINERLELTFN
PYTTQIEPHDYIAELFDAVKRFNTILIDFNRDIWQYISLGYFKQRLKDGEVGSSTMPHKVNPIDFENSEGNLGVANAMLA
HLGEKLPISRMQRDLSDSTVLRNIGVGLAQSMIAYDACLKGVSKLELNAQRLNDDLDNAQEVLAEPIQTVMRRYRVENPY
EKLKALTRGNAMTREAMLTFVDGDELSAVSESDKNSLREMTPATYIGNAAEQARTIKEWIAKI

Sequences:

>Translated_463_residues
MNLLTALSPIDGRYASKADSLRPYLSEFGLIKARVTVEIRWLQSLADNSAIGELAEFDTNTNAFLNAIVENFSEADAQAI
KDIEATTNHDVKAVEYFIKDKFRGQAALEDSLEFIHFACTSEDINNLSYALMLKDSREIVVAKMQEVTDSIVNLAIIHAD
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQVGQVELLGKINGAVGNYNAHYASYPDVDWQNHAEKFINERLELTFN
PYTTQIEPHDYIAELFDAVKRFNTILIDFNRDIWQYISLGYFKQRLKDGEVGSSTMPHKVNPIDFENSEGNLGVANAMLA
HLGEKLPISRMQRDLSDSTVLRNIGVGLAQSMIAYDACLKGVSKLELNAQRLNDDLDNAQEVLAEPIQTVMRRYRVENPY
EKLKALTRGNAMTREAMLTFVDGDELSAVSESDKNSLREMTPATYIGNAAEQARTIKEWIAKI
>Mature_463_residues
MNLLTALSPIDGRYASKADSLRPYLSEFGLIKARVTVEIRWLQSLADNSAIGELAEFDTNTNAFLNAIVENFSEADAQAI
KDIEATTNHDVKAVEYFIKDKFRGQAALEDSLEFIHFACTSEDINNLSYALMLKDSREIVVAKMQEVTDSIVNLAIIHAD
QPMLSRTHGQTASPTTLGKEMANVAYRLARQIKQVGQVELLGKINGAVGNYNAHYASYPDVDWQNHAEKFINERLELTFN
PYTTQIEPHDYIAELFDAVKRFNTILIDFNRDIWQYISLGYFKQRLKDGEVGSSTMPHKVNPIDFENSEGNLGVANAMLA
HLGEKLPISRMQRDLSDSTVLRNIGVGLAQSMIAYDACLKGVSKLELNAQRLNDDLDNAQEVLAEPIQTVMRRYRVENPY
EKLKALTRGNAMTREAMLTFVDGDELSAVSESDKNSLREMTPATYIGNAAEQARTIKEWIAKI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=450, Percent_Identity=62.2222222222222, Blast_Score=580, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51900; Mature: 51900

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLTALSPIDGRYASKADSLRPYLSEFGLIKARVTVEIRWLQSLADNSAIGELAEFDTN
CCCHHHCCCCCCCHHCCHHHHHHHHHHCCEEEEEEEEEHHHHHHHHCCHHHHHHHHCCCC
TNAFLNAIVENFSEADAQAIKDIEATTNHDVKAVEYFIKDKFRGQAALEDSLEFIHFACT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEC
SEDINNLSYALMLKDSREIVVAKMQEVTDSIVNLAIIHADQPMLSRTHGQTASPTTLGKE
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCCHHHHHH
MANVAYRLARQIKQVGQVELLGKINGAVGNYNAHYASYPDVDWQNHAEKFINERLELTFN
HHHHHHHHHHHHHHHCCEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEC
PYTTQIEPHDYIAELFDAVKRFNTILIDFNRDIWQYISLGYFKQRLKDGEVGSSTMPHKV
CCCCCCCCHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NPIDFENSEGNLGVANAMLAHLGEKLPISRMQRDLSDSTVLRNIGVGLAQSMIAYDACLK
CCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GVSKLELNAQRLNDDLDNAQEVLAEPIQTVMRRYRVENPYEKLKALTRGNAMTREAMLTF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHEEE
VDGDELSAVSESDKNSLREMTPATYIGNAAEQARTIKEWIAKI
ECCCHHHHHCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLLTALSPIDGRYASKADSLRPYLSEFGLIKARVTVEIRWLQSLADNSAIGELAEFDTN
CCCHHHCCCCCCCHHCCHHHHHHHHHHCCEEEEEEEEEHHHHHHHHCCHHHHHHHHCCCC
TNAFLNAIVENFSEADAQAIKDIEATTNHDVKAVEYFIKDKFRGQAALEDSLEFIHFACT
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHEEC
SEDINNLSYALMLKDSREIVVAKMQEVTDSIVNLAIIHADQPMLSRTHGQTASPTTLGKE
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHCCCCCCCCHHHHHH
MANVAYRLARQIKQVGQVELLGKINGAVGNYNAHYASYPDVDWQNHAEKFINERLELTFN
HHHHHHHHHHHHHHHCCEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEC
PYTTQIEPHDYIAELFDAVKRFNTILIDFNRDIWQYISLGYFKQRLKDGEVGSSTMPHKV
CCCCCCCCHHHHHHHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NPIDFENSEGNLGVANAMLAHLGEKLPISRMQRDLSDSTVLRNIGVGLAQSMIAYDACLK
CCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
GVSKLELNAQRLNDDLDNAQEVLAEPIQTVMRRYRVENPYEKLKALTRGNAMTREAMLTF
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHEEE
VDGDELSAVSESDKNSLREMTPATYIGNAAEQARTIKEWIAKI
ECCCHHHHHCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]