Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 93005953

GI number: 93005953

Start: 1355876

End: 1358026

Strand: Reverse

Name: dnaG [H]

Synonym: Pcryo_1125

Alternate gene names: 93005953

Gene position: 1358026-1355876 (Counterclockwise)

Preceding gene: 93005954

Following gene: 93005952

Centisome position: 44.38

GC content: 43.47

Gene sequence:

>2151_bases
ATGAGTATCCCAAATCACATTCTTGAACAATTAAATAGCCAAGCTGATTTAGTCAGTATCATTGGGCGTCATACTACGCT
TAAACGTGCTGGTAGTGAGTTTAAAGGCTGCTGTCCTTTTCATGGTGAAAAGTCGCCGTCTTTTTATGTCAATCCACAAA
AAAACATCTACCACTGCTTTGGCTGTAGTGTCGGTGGTAACGCCATCAGTTTTTTACGTGATTATGAAAATCTCACCTTT
ATCGAAGCGGTCAATGAGCTGTCCAAGCAGACGGGCATTGAAGTCCCAAAAGAAGAACAGCAAAACGTCAGCTATCAGCG
TGCCCCAGTTAAACCAAAATCCAAGCCAAAATCTGCTGTTAAGCCAGCAACATCGGCAACGCATCTGCGAAAAAATGATA
GTCAGCCAGTAAGCAATAATCAGTCATCCATAATCATGCCAGCTGATGATACCCAGCCGCCCATTTATGATGACCACCAT
AGTTATGACAATTACCCACCGTTAGATGCTTACGATGCCGTTCCTTATGTAATGGATGGCTATGATGCTCATTATCAAGA
TGACAATGGCTATCCGCCTGCATGGTTAACCGGTGATGATAATACTGCTCTCTACAATGATAATAGTATCTCTGATAAAG
ATGGCAACTTATATGAGCTGCTAGAGCAGATACAGCAGTTTTATCAACATAATCTCAGCATTCACCCTCATGCTAAGCAT
TACTTCTTATCGCGCGGTATTAGCGATGAAATCTTCGAAACTTTTGGCTTGGGCTACGCGCCTTTTGGTTGGCAACATCT
TGAGCATCAGTTCCCACAAGATATCGAAGGTCTAAAGGCGCTTGGTTTAGTACGCCAATCAGAATCTGGACGCGATTATG
ACCTGCTCCGTGATCGGGTTATTTTCCCAATTCGAGACAATCAAGGACGCACGATTGGTTTTGGTGGTCGTGCGCTTGAT
GATGAAGTGAAACCCAAATATATCAACTCCTCCGACTCGCCCGTCTTTCATAAGCAGCACGTATTATATGGCTATTATGA
GTCACGCCAAAAGCGTGCTGACAACTGGCTGGTAGTCGAAGGCTATATGGATGTGATTGCTCTTTATCAAGCAGGTATCT
ATGGTGCCATTGCGTCGATGGGTACGGCGATTAATGAGAGCCAAATCTCACGGCTATTAACGCTAAATCCAACATTAACC
TTAAGCTTTGATGGTGATAGCGCCGGACAAAAAGCCGCTTGGCGCACGCTTGAAGTGGCTTTGCCAGTGCTCAGTGATGA
TAAAGAGCTAAGATTTTTAACACTGCCCAATAATCATGACCCTGATACCTTTATCAAAAGTCAGGGCGGTGATGCCATGC
GTGAGCAAATAGCTAATGCCATGCCGCTATCACAGTATATTTTTGCCTATTTGAGTGAACGTCATGATTTGACTTTGGTA
GAAGGTAAAGCCAAGCTCATGTCCCAAGTACGCGCATTAACCATGGCATTACCCAAAGGCAGTAGTTTTAAGTATTTGCT
TAACAATGACATTTATCAAAAGCTAGGCGGTAAACGCAGTCAGAATGTCGAGGCAAAAGACGCGCTGCTAGATTTTGATG
GCGACATGACCATCAGCCAGCAGTTGCAGCTGTGCTTTTTATTCCAGCCGCGCGCACTACTTGAAGACCCTGTTGAGTGG
ATCTGGCAACAATCAGGGCTTATTAACTTAAAATTACCGGCTCATATCCAGAAAAAATCTGCTAACGATGCATTACGTGC
AATTACATGGGAAGATTTGCAAGATACGGCGCTATTAGACATAGTCACTGCCATCAAGCGCTGCATGAAGCATCTGCCAA
TAGATGCTAATGCGGCAGCCCATTTTGTATTATCAAATCTTAGTCCAATGAACCAAGCTACTTTGAGTAATAGCTGGGGT
GGGTTTTATAAAGCATTAACTACAAGGAATGTACTAAGTCTTGATGATTTGATTGAAGAGCTGGTCAGTCAGCTACTCGA
ACGTAATATAAAAAAGAAGATGCAAGAGCTGATAAAAAACCCTGACCATATCAAACAAGCGATAGTTAATATGCAAGCAC
AGCATCTAAGTAACTGGATACGCGCACAACGTGCCGAACGCTCATCGCAAATGGCGACGGTCAAATCTTAA

Upstream 100 bases:

>100_bases
AAATAAGGTCAACGATACGGTTGGCCTTATTTTTTCTGTTTTGGTTTTTGCATTATTTTGAGCATCAACAATATTGCACC
TATGGTAAACTGTTTTTCGC

Downstream 100 bases:

>100_bases
TATGGTCATTAGATTTTATTTATAAAACAGCTGCCCTTTAAAAGGGTCGACATTGTCCCCACTATTAATGATTATGCACT
GTTGTCTTAGCTTATTAAAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MSIPNHILEQLNSQADLVSIIGRHTTLKRAGSEFKGCCPFHGEKSPSFYVNPQKNIYHCFGCSVGGNAISFLRDYENLTF
IEAVNELSKQTGIEVPKEEQQNVSYQRAPVKPKSKPKSAVKPATSATHLRKNDSQPVSNNQSSIIMPADDTQPPIYDDHH
SYDNYPPLDAYDAVPYVMDGYDAHYQDDNGYPPAWLTGDDNTALYNDNSISDKDGNLYELLEQIQQFYQHNLSIHPHAKH
YFLSRGISDEIFETFGLGYAPFGWQHLEHQFPQDIEGLKALGLVRQSESGRDYDLLRDRVIFPIRDNQGRTIGFGGRALD
DEVKPKYINSSDSPVFHKQHVLYGYYESRQKRADNWLVVEGYMDVIALYQAGIYGAIASMGTAINESQISRLLTLNPTLT
LSFDGDSAGQKAAWRTLEVALPVLSDDKELRFLTLPNNHDPDTFIKSQGGDAMREQIANAMPLSQYIFAYLSERHDLTLV
EGKAKLMSQVRALTMALPKGSSFKYLLNNDIYQKLGGKRSQNVEAKDALLDFDGDMTISQQLQLCFLFQPRALLEDPVEW
IWQQSGLINLKLPAHIQKKSANDALRAITWEDLQDTALLDIVTAIKRCMKHLPIDANAAAHFVLSNLSPMNQATLSNSWG
GFYKALTTRNVLSLDDLIEELVSQLLERNIKKKMQELIKNPDHIKQAIVNMQAQHLSNWIRAQRAERSSQMATVKS

Sequences:

>Translated_716_residues
MSIPNHILEQLNSQADLVSIIGRHTTLKRAGSEFKGCCPFHGEKSPSFYVNPQKNIYHCFGCSVGGNAISFLRDYENLTF
IEAVNELSKQTGIEVPKEEQQNVSYQRAPVKPKSKPKSAVKPATSATHLRKNDSQPVSNNQSSIIMPADDTQPPIYDDHH
SYDNYPPLDAYDAVPYVMDGYDAHYQDDNGYPPAWLTGDDNTALYNDNSISDKDGNLYELLEQIQQFYQHNLSIHPHAKH
YFLSRGISDEIFETFGLGYAPFGWQHLEHQFPQDIEGLKALGLVRQSESGRDYDLLRDRVIFPIRDNQGRTIGFGGRALD
DEVKPKYINSSDSPVFHKQHVLYGYYESRQKRADNWLVVEGYMDVIALYQAGIYGAIASMGTAINESQISRLLTLNPTLT
LSFDGDSAGQKAAWRTLEVALPVLSDDKELRFLTLPNNHDPDTFIKSQGGDAMREQIANAMPLSQYIFAYLSERHDLTLV
EGKAKLMSQVRALTMALPKGSSFKYLLNNDIYQKLGGKRSQNVEAKDALLDFDGDMTISQQLQLCFLFQPRALLEDPVEW
IWQQSGLINLKLPAHIQKKSANDALRAITWEDLQDTALLDIVTAIKRCMKHLPIDANAAAHFVLSNLSPMNQATLSNSWG
GFYKALTTRNVLSLDDLIEELVSQLLERNIKKKMQELIKNPDHIKQAIVNMQAQHLSNWIRAQRAERSSQMATVKS
>Mature_715_residues
SIPNHILEQLNSQADLVSIIGRHTTLKRAGSEFKGCCPFHGEKSPSFYVNPQKNIYHCFGCSVGGNAISFLRDYENLTFI
EAVNELSKQTGIEVPKEEQQNVSYQRAPVKPKSKPKSAVKPATSATHLRKNDSQPVSNNQSSIIMPADDTQPPIYDDHHS
YDNYPPLDAYDAVPYVMDGYDAHYQDDNGYPPAWLTGDDNTALYNDNSISDKDGNLYELLEQIQQFYQHNLSIHPHAKHY
FLSRGISDEIFETFGLGYAPFGWQHLEHQFPQDIEGLKALGLVRQSESGRDYDLLRDRVIFPIRDNQGRTIGFGGRALDD
EVKPKYINSSDSPVFHKQHVLYGYYESRQKRADNWLVVEGYMDVIALYQAGIYGAIASMGTAINESQISRLLTLNPTLTL
SFDGDSAGQKAAWRTLEVALPVLSDDKELRFLTLPNNHDPDTFIKSQGGDAMREQIANAMPLSQYIFAYLSERHDLTLVE
GKAKLMSQVRALTMALPKGSSFKYLLNNDIYQKLGGKRSQNVEAKDALLDFDGDMTISQQLQLCFLFQPRALLEDPVEWI
WQQSGLINLKLPAHIQKKSANDALRAITWEDLQDTALLDIVTAIKRCMKHLPIDANAAAHFVLSNLSPMNQATLSNSWGG
FYKALTTRNVLSLDDLIEELVSQLLERNIKKKMQELIKNPDHIKQAIVNMQAQHLSNWIRAQRAERSSQMATVKS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=307, Percent_Identity=40.7166123778502, Blast_Score=248, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 80759; Mature: 80628

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNHILEQLNSQADLVSIIGRHTTLKRAGSEFKGCCPFHGEKSPSFYVNPQKNIYHCF
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEEE
GCSVGGNAISFLRDYENLTFIEAVNELSKQTGIEVPKEEQQNVSYQRAPVKPKSKPKSAV
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCCCCCHHHC
KPATSATHLRKNDSQPVSNNQSSIIMPADDTQPPIYDDHHSYDNYPPLDAYDAVPYVMDG
CCCCHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
YDAHYQDDNGYPPAWLTGDDNTALYNDNSISDKDGNLYELLEQIQQFYQHNLSIHPHAKH
CCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
YFLSRGISDEIFETFGLGYAPFGWQHLEHQFPQDIEGLKALGLVRQSESGRDYDLLRDRV
HHHHCCCCHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCE
IFPIRDNQGRTIGFGGRALDDEVKPKYINSSDSPVFHKQHVLYGYYESRQKRADNWLVVE
EEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHCCCCEEEEH
GYMDVIALYQAGIYGAIASMGTAINESQISRLLTLNPTLTLSFDGDSAGQKAAWRTLEVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCEEEEEECCCCCCCHHHHHHHEEE
LPVLSDDKELRFLTLPNNHDPDTFIKSQGGDAMREQIANAMPLSQYIFAYLSERHDLTLV
ECCCCCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEE
EGKAKLMSQVRALTMALPKGSSFKYLLNNDIYQKLGGKRSQNVEAKDALLDFDGDMTISQ
CCHHHHHHHHHHHHHHCCCCCCCEEHHCCHHHHHHCCCCCCCCCHHHHEEECCCCCCHHH
QLQLCFLFQPRALLEDPVEWIWQQSGLINLKLPAHIQKKSANDALRAITWEDLQDTALLD
HCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHEEEHHCCHHHHHHH
IVTAIKRCMKHLPIDANAAAHFVLSNLSPMNQATLSNSWGGFYKALTTRNVLSLDDLIEE
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHH
LVSQLLERNIKKKMQELIKNPDHIKQAIVNMQAQHLSNWIRAQRAERSSQMATVKS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIPNHILEQLNSQADLVSIIGRHTTLKRAGSEFKGCCPFHGEKSPSFYVNPQKNIYHCF
CCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCEEEEE
GCSVGGNAISFLRDYENLTFIEAVNELSKQTGIEVPKEEQQNVSYQRAPVKPKSKPKSAV
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHCCCEEECCCCCCCCCHHHC
KPATSATHLRKNDSQPVSNNQSSIIMPADDTQPPIYDDHHSYDNYPPLDAYDAVPYVMDG
CCCCHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCC
YDAHYQDDNGYPPAWLTGDDNTALYNDNSISDKDGNLYELLEQIQQFYQHNLSIHPHAKH
CCCCCCCCCCCCCEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHH
YFLSRGISDEIFETFGLGYAPFGWQHLEHQFPQDIEGLKALGLVRQSESGRDYDLLRDRV
HHHHCCCCHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCE
IFPIRDNQGRTIGFGGRALDDEVKPKYINSSDSPVFHKQHVLYGYYESRQKRADNWLVVE
EEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEHHHHEEHHHHHHHHCCCCEEEEH
GYMDVIALYQAGIYGAIASMGTAINESQISRLLTLNPTLTLSFDGDSAGQKAAWRTLEVA
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCEEEEEECCCCCCCHHHHHHHEEE
LPVLSDDKELRFLTLPNNHDPDTFIKSQGGDAMREQIANAMPLSQYIFAYLSERHDLTLV
ECCCCCCCCEEEEECCCCCCCCHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEEE
EGKAKLMSQVRALTMALPKGSSFKYLLNNDIYQKLGGKRSQNVEAKDALLDFDGDMTISQ
CCHHHHHHHHHHHHHHCCCCCCCEEHHCCHHHHHHCCCCCCCCCHHHHEEECCCCCCHHH
QLQLCFLFQPRALLEDPVEWIWQQSGLINLKLPAHIQKKSANDALRAITWEDLQDTALLD
HCCEEEEECCHHHHHHHHHHHHHCCCEEEEECCCHHHHCCCCHHHHEEEHHCCHHHHHHH
IVTAIKRCMKHLPIDANAAAHFVLSNLSPMNQATLSNSWGGFYKALTTRNVLSLDDLIEE
HHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHH
LVSQLLERNIKKKMQELIKNPDHIKQAIVNMQAQHLSNWIRAQRAERSSQMATVKS
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]