Click here to switch to the map view.

The map label for this gene is psdht [H]

Identifier: 93005808

GI number: 93005808

Start: 1168943

End: 1169923

Strand: Reverse

Name: psdht [H]

Synonym: Pcryo_0980

Alternate gene names: 93005808

Gene position: 1169923-1168943 (Counterclockwise)

Preceding gene: 93005809

Following gene: 93005807

Centisome position: 38.23

GC content: 45.67

Gene sequence:

>981_bases
ATGATAACAAATGATAATGGATTAGTGATTCCAACCCTTGATGATATGTTAGAAGCGCATGAGCGCATCAAACCATATAT
CCATCGCACCCCTGTTCTGACCTCTCGCTTTTTAAATGAGCTGGCTGGCTGTGAGATGTTCTTTAAATGTGAGAACTTTC
AAAAAGCGGGTGCATTTAAAGTACGTGGCGCCTCAAATGCAGTGTTTGGCCTCTCTGACGAAGATGCCAAAAATGGCGTT
TGTACACACAGCTCAGGTAACCATGCCCTATCTTTATCTTATGCTGCAGGCCAGCGTGGTATACCATGTAATGTGGTGAT
GCCGCATAGCGCGCCTGAAGCTAAAAAAGCCGCGGTACGTGGCTATGGCGGTATCATCACCGAATGTGAGCCATCAACCA
CATCGCGTGAAGAAGTCTTTGCCAAAGTTCAAGCAGAAACAGGCGGCGACTTTGTCCATCCGTACAATGATCCACGCGTT
ATCGCTGGACAAGCCACTTGCTCACGTGAGTTTTTAGAACAAATGGAAGAGATTGGTGAAAAACCTGATATGGTTGTGGC
ACCCATTGGTGGTGGCGGTATGATTTCAGGTACTTGTTTGACGTTGTCGAACCTAGCACCAGATGTCAAAATCTATGCAG
CAGAGCCATTAAACGCTGATGACGCTGCACGCTCATTTAAAGCCGGTCATATTATCGCTGATGATGCACCAAACACGGTT
GCCGATGGTTTAAAAGTACCGCTGAAGGATTTGACTTGGCATTTTGTTAGCAATTATGTGACTGATATTTTGACCGCGAC
TGAAGAAGAAATCGTTGAAGCGATGAAACTGACTTGGACGCATATGAAAATCATCATCGAACCAAGCTGTGCCGTGCCGC
TTGCTGTCATCCTGAAGAACAAAGACGTTTTTGCCGGTAAAAAGGTTGGCGTCATCATCACTGGTGGTAACGTTGATCTT
GACAAGTTGCCTTGGAATTAA

Upstream 100 bases:

>100_bases
GCAAGAGTATTATCGCCACAGCGCACCAACTAAATAAAATTTATTAGATAACAAGCTATTAAATAACAAGAGTTAAACAA
AAGATGAAGGACTATTTTTT

Downstream 100 bases:

>100_bases
TGATCGGCAACTAAATATCAGTAATTAGGTATCAATAACTGATTACAAATGAAGCGTTAACTAAGCGCCAGTAATGCGCA
AGTTTAAACGGCTTTTTATA

Product: pyridoxal-5'-phosphate-dependent enzyme subunit beta

Products: @OXOB02.txt*Oxobutanoate!; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MITNDNGLVIPTLDDMLEAHERIKPYIHRTPVLTSRFLNELAGCEMFFKCENFQKAGAFKVRGASNAVFGLSDEDAKNGV
CTHSSGNHALSLSYAAGQRGIPCNVVMPHSAPEAKKAAVRGYGGIITECEPSTTSREEVFAKVQAETGGDFVHPYNDPRV
IAGQATCSREFLEQMEEIGEKPDMVVAPIGGGGMISGTCLTLSNLAPDVKIYAAEPLNADDAARSFKAGHIIADDAPNTV
ADGLKVPLKDLTWHFVSNYVTDILTATEEEIVEAMKLTWTHMKIIIEPSCAVPLAVILKNKDVFAGKKVGVIITGGNVDL
DKLPWN

Sequences:

>Translated_326_residues
MITNDNGLVIPTLDDMLEAHERIKPYIHRTPVLTSRFLNELAGCEMFFKCENFQKAGAFKVRGASNAVFGLSDEDAKNGV
CTHSSGNHALSLSYAAGQRGIPCNVVMPHSAPEAKKAAVRGYGGIITECEPSTTSREEVFAKVQAETGGDFVHPYNDPRV
IAGQATCSREFLEQMEEIGEKPDMVVAPIGGGGMISGTCLTLSNLAPDVKIYAAEPLNADDAARSFKAGHIIADDAPNTV
ADGLKVPLKDLTWHFVSNYVTDILTATEEEIVEAMKLTWTHMKIIIEPSCAVPLAVILKNKDVFAGKKVGVIITGGNVDL
DKLPWN
>Mature_326_residues
MITNDNGLVIPTLDDMLEAHERIKPYIHRTPVLTSRFLNELAGCEMFFKCENFQKAGAFKVRGASNAVFGLSDEDAKNGV
CTHSSGNHALSLSYAAGQRGIPCNVVMPHSAPEAKKAAVRGYGGIITECEPSTTSREEVFAKVQAETGGDFVHPYNDPRV
IAGQATCSREFLEQMEEIGEKPDMVVAPIGGGGMISGTCLTLSNLAPDVKIYAAEPLNADDAARSFKAGHIIADDAPNTV
ADGLKVPLKDLTWHFVSNYVTDILTATEEEIVEAMKLTWTHMKIIIEPSCAVPLAVILKNKDVFAGKKVGVIITGGNVDL
DKLPWN

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=322, Percent_Identity=45.0310559006211, Blast_Score=267, Evalue=9e-72,
Organism=Homo sapiens, GI33469958, Length=282, Percent_Identity=26.241134751773, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1789505, Length=315, Percent_Identity=33.968253968254, Blast_Score=154, Evalue=6e-39,
Organism=Escherichia coli, GI1790207, Length=302, Percent_Identity=29.4701986754967, Blast_Score=118, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17508781, Length=314, Percent_Identity=36.3057324840764, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI71991565, Length=309, Percent_Identity=33.6569579288026, Blast_Score=140, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI17537387, Length=313, Percent_Identity=31.9488817891374, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6322631, Length=316, Percent_Identity=41.7721518987342, Blast_Score=245, Evalue=6e-66,
Organism=Saccharomyces cerevisiae, GI6320930, Length=306, Percent_Identity=28.7581699346405, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319788, Length=285, Percent_Identity=23.859649122807, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI21355833, Length=315, Percent_Identity=31.4285714285714, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 35100; Mature: 35100

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITNDNGLVIPTLDDMLEAHERIKPYIHRTPVLTSRFLNELAGCEMFFKCENFQKAGAFK
CEECCCCEEEECHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCEE
VRGASNAVFGLSDEDAKNGVCTHSSGNHALSLSYAAGQRGIPCNVVMPHSAPEAKKAAVR
EECCCCEEEECCCCCCCCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHH
GYGGIITECEPSTTSREEVFAKVQAETGGDFVHPYNDPRVIAGQATCSREFLEQMEEIGE
CCCCEEEECCCCCCCHHHHHEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCC
KPDMVVAPIGGGGMISGTCLTLSNLAPDVKIYAAEPLNADDAARSFKAGHIIADDAPNTV
CCCEEEEECCCCCEECCCEEECCCCCCCEEEEEECCCCCHHHHHHHCCCEEEECCCCCHH
ADGLKVPLKDLTWHFVSNYVTDILTATEEEIVEAMKLTWTHMKIIIEPSCAVPLAVILKN
HCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEECCCCCCCEEEEEEC
KDVFAGKKVGVIITGGNVDLDKLPWN
CCEECCCEEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MITNDNGLVIPTLDDMLEAHERIKPYIHRTPVLTSRFLNELAGCEMFFKCENFQKAGAFK
CEECCCCEEEECHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHCCEEEEEECCCCCCCCEE
VRGASNAVFGLSDEDAKNGVCTHSSGNHALSLSYAAGQRGIPCNVVMPHSAPEAKKAAVR
EECCCCEEEECCCCCCCCCEEECCCCCCEEEEEECCCCCCCCEEEEECCCCCHHHHHHHH
GYGGIITECEPSTTSREEVFAKVQAETGGDFVHPYNDPRVIAGQATCSREFLEQMEEIGE
CCCCEEEECCCCCCCHHHHHEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCC
KPDMVVAPIGGGGMISGTCLTLSNLAPDVKIYAAEPLNADDAARSFKAGHIIADDAPNTV
CCCEEEEECCCCCEECCCEEECCCCCCCEEEEEECCCCCHHHHHHHCCCEEEECCCCCHH
ADGLKVPLKDLTWHFVSNYVTDILTATEEEIVEAMKLTWTHMKIIIEPSCAVPLAVILKN
HCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEEEEEEEECCCCCCCEEEEEEC
KDVFAGKKVGVIITGGNVDLDKLPWN
CCEECCCEEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = @OXOB02.txt*oxobutanoate! + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]