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The map label for this gene is puuB [H]

Identifier: 93005725

GI number: 93005725

Start: 1062655

End: 1063941

Strand: Direct

Name: puuB [H]

Synonym: Pcryo_0897

Alternate gene names: 93005725

Gene position: 1062655-1063941 (Clockwise)

Preceding gene: 93005724

Following gene: 93005726

Centisome position: 34.73

GC content: 48.72

Gene sequence:

>1287_bases
ATGTTGCAAGAGCAATGTTTGTGGAGCGTCACTGCGCCCAAAACTCACCACTACGATACCCTAACAAGCGAAACGCAAAC
ACGAGTTTGTATCATCGGCGGTGGTTATACTGGACTGTCTGCTGCGATCCATCTGGCGGAGCAAGGAGTGGCGGTGGTGT
TACTAGAGAGTAAGATCATCGGTAGCGGCGGCTCAGGACGCAATGTGGGTTTTGTGAATGCCGGTACTTGGGCTCGTCCT
GATGACCTCAATAAAATCATGGGAGAAGAGGTTGGTGAACGTTTAGGGACTGCGCTCGGTCAGGCGCCAAGTCTCGTGTT
TGACATCATCGATCGCCATCATATTGATGCAAAGGATACGCGTACGGGCAATATCCATATGGCACACAATGCCAAGGGTG
AAGCCGATGTGGACATCCGTTATGATCAGTTGATACGTCGCGGTGTTCATGCCGAGGTGTTAACCGGTGCGCGCTGCCAT
GAATACTGCGGGACAACCAGTATCAATAAGGCTCTGCTCGATCATCGTGCGGGCACTATTAACCCATCCGCATACGTCAA
TGGCTTAGCGGCAGCCGCGACTAAATTAGGCGTACGTATCTATGAGCACTCGGCAGTAACCTCAGTTGAGAAATCTGATG
GTAATTGGTATGTGCGTACGGCTGATGCGGCAGTGAAAGCTGAAAAAGTCATCATCGCGACCAATGCTTATACCGAAGGC
GAATGGACAGATATTAAGAAGACGTTCTATTTGGTTGATTATTATCAAATTGCCTCTGAGCCATTGACCGGTGAAGTGGC
AGATCGAATATTGCCTTATAAAACAGGCGCTTGGGATACGCGATTGGCATTGTCCAGTATTCGCCGTGATAAAGAGGGTC
GTTTACTCCTTGGCACCGTCGGTGGCAAAGCCATGAAGTCCAAAACCTTCTATCAAAACTGGGCGAATGTCGTACAAAAG
AGCTATTTCCCAGATCTGCCTAAATTTAAGTGGCAGTATGAGTGGTACGGTCACTTTGGCTTTACGCAAGACCATATCAT
GCGTATCTTTGAGCCAGAAGACGGTATCCTTGCCGCGACTGCATACAATGGGCGTGGGATTACCACAGGTACTATGATTG
GTAAATGCTTTGCCGATTATATTATCTCAGGTGACCGCGATGTCTTGCCATTACCTTTTAAGACCGTTAAAGAAGCTAAG
GTGAGCATGTCGGGACTGCGTTCGGGCTATACGGAGCTGGGGCTGACTTTGTATCATATGGGTCAATGTCTCAAAATCAT
AGCCTAG

Upstream 100 bases:

>100_bases
TCGTCTTAAATAAATTTATTCCTCGTGAAAAACCGACTGTCATCGATAACACAAGCACGCTCTCTACCGTAGAAAACGTA
TAATTTAGAAGGAACTTGAG

Downstream 100 bases:

>100_bases
TTTTTTGACGCAGGGCGTTGTGTTTATTTATTGCCGCCGCTGATCTGGCAGAGAGATTAAGTGAACGGCACACTGCTTTT
ATTTATTTGGAGTGTATATT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MLQEQCLWSVTAPKTHHYDTLTSETQTRVCIIGGGYTGLSAAIHLAEQGVAVVLLESKIIGSGGSGRNVGFVNAGTWARP
DDLNKIMGEEVGERLGTALGQAPSLVFDIIDRHHIDAKDTRTGNIHMAHNAKGEADVDIRYDQLIRRGVHAEVLTGARCH
EYCGTTSINKALLDHRAGTINPSAYVNGLAAAATKLGVRIYEHSAVTSVEKSDGNWYVRTADAAVKAEKVIIATNAYTEG
EWTDIKKTFYLVDYYQIASEPLTGEVADRILPYKTGAWDTRLALSSIRRDKEGRLLLGTVGGKAMKSKTFYQNWANVVQK
SYFPDLPKFKWQYEWYGHFGFTQDHIMRIFEPEDGILAATAYNGRGITTGTMIGKCFADYIISGDRDVLPLPFKTVKEAK
VSMSGLRSGYTELGLTLYHMGQCLKIIA

Sequences:

>Translated_428_residues
MLQEQCLWSVTAPKTHHYDTLTSETQTRVCIIGGGYTGLSAAIHLAEQGVAVVLLESKIIGSGGSGRNVGFVNAGTWARP
DDLNKIMGEEVGERLGTALGQAPSLVFDIIDRHHIDAKDTRTGNIHMAHNAKGEADVDIRYDQLIRRGVHAEVLTGARCH
EYCGTTSINKALLDHRAGTINPSAYVNGLAAAATKLGVRIYEHSAVTSVEKSDGNWYVRTADAAVKAEKVIIATNAYTEG
EWTDIKKTFYLVDYYQIASEPLTGEVADRILPYKTGAWDTRLALSSIRRDKEGRLLLGTVGGKAMKSKTFYQNWANVVQK
SYFPDLPKFKWQYEWYGHFGFTQDHIMRIFEPEDGILAATAYNGRGITTGTMIGKCFADYIISGDRDVLPLPFKTVKEAK
VSMSGLRSGYTELGLTLYHMGQCLKIIA
>Mature_428_residues
MLQEQCLWSVTAPKTHHYDTLTSETQTRVCIIGGGYTGLSAAIHLAEQGVAVVLLESKIIGSGGSGRNVGFVNAGTWARP
DDLNKIMGEEVGERLGTALGQAPSLVFDIIDRHHIDAKDTRTGNIHMAHNAKGEADVDIRYDQLIRRGVHAEVLTGARCH
EYCGTTSINKALLDHRAGTINPSAYVNGLAAAATKLGVRIYEHSAVTSVEKSDGNWYVRTADAAVKAEKVIIATNAYTEG
EWTDIKKTFYLVDYYQIASEPLTGEVADRILPYKTGAWDTRLALSSIRRDKEGRLLLGTVGGKAMKSKTFYQNWANVVQK
SYFPDLPKFKWQYEWYGHFGFTQDHIMRIFEPEDGILAATAYNGRGITTGTMIGKCFADYIISGDRDVLPLPFKTVKEAK
VSMSGLRSGYTELGLTLYHMGQCLKIIA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=419, Percent_Identity=31.0262529832936, Blast_Score=149, Evalue=5e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47094; Mature: 47094

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQEQCLWSVTAPKTHHYDTLTSETQTRVCIIGGGYTGLSAAIHLAEQGVAVVLLESKII
CCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEEEEEEE
GSGGSGRNVGFVNAGTWARPDDLNKIMGEEVGERLGTALGQAPSLVFDIIDRHHIDAKDT
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
RTGNIHMAHNAKGEADVDIRYDQLIRRGVHAEVLTGARCHEYCGTTSINKALLDHRAGTI
CCCCEEEEECCCCCCEEEEEHHHHHHCCCCHHHHCCCHHHHHCCCCHHHHHHHHCCCCCC
NPSAYVNGLAAAATKLGVRIYEHSAVTSVEKSDGNWYVRTADAAVKAEKVIIATNAYTEG
CHHHHHHHHHHHHHHHCEEEEECHHHCEEEECCCCEEEEECCCCEEEEEEEEEECCCCCC
EWTDIKKTFYLVDYYQIASEPLTGEVADRILPYKTGAWDTRLALSSIRRDKEGRLLLGTV
CCCCCEEEEEEEEHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEC
GGKAMKSKTFYQNWANVVQKSYFPDLPKFKWQYEWYGHFGFTQDHIMRIFEPEDGILAAT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHEEEECCCCCEEEEE
AYNGRGITTGTMIGKCFADYIISGDRDVLPLPFKTVKEAKVSMSGLRSGYTELGLTLYHM
EECCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GQCLKIIA
HHHHHHCC
>Mature Secondary Structure
MLQEQCLWSVTAPKTHHYDTLTSETQTRVCIIGGGYTGLSAAIHLAEQGVAVVLLESKII
CCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEEEEEEEEEE
GSGGSGRNVGFVNAGTWARPDDLNKIMGEEVGERLGTALGQAPSLVFDIIDRHHIDAKDT
CCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCC
RTGNIHMAHNAKGEADVDIRYDQLIRRGVHAEVLTGARCHEYCGTTSINKALLDHRAGTI
CCCCEEEEECCCCCCEEEEEHHHHHHCCCCHHHHCCCHHHHHCCCCHHHHHHHHCCCCCC
NPSAYVNGLAAAATKLGVRIYEHSAVTSVEKSDGNWYVRTADAAVKAEKVIIATNAYTEG
CHHHHHHHHHHHHHHHCEEEEECHHHCEEEECCCCEEEEECCCCEEEEEEEEEECCCCCC
EWTDIKKTFYLVDYYQIASEPLTGEVADRILPYKTGAWDTRLALSSIRRDKEGRLLLGTV
CCCCCEEEEEEEEHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEC
GGKAMKSKTFYQNWANVVQKSYFPDLPKFKWQYEWYGHFGFTQDHIMRIFEPEDGILAAT
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCHHHHEEEECCCCCEEEEE
AYNGRGITTGTMIGKCFADYIISGDRDVLPLPFKTVKEAKVSMSGLRSGYTELGLTLYHM
EECCCCCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
GQCLKIIA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]