Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 93005720

GI number: 93005720

Start: 1055057

End: 1056034

Strand: Direct

Name: gcvA [H]

Synonym: Pcryo_0892

Alternate gene names: 93005720

Gene position: 1055057-1056034 (Clockwise)

Preceding gene: 93005719

Following gene: 93005721

Centisome position: 34.48

GC content: 46.42

Gene sequence:

>978_bases
ATGAAAACTGATACCAATCCTTCGCTTGCGTTTAGAAGATACCCTTCGACGACTGCGCTGCAATGCTTCGAGACAGCGGC
TCGTCACCTAAGTTTTACTAATGCCGCCTTAGAGCTGCATATGACGCAAAGTGCGATAAGCAAGCAAGTGGCGCAGTTAG
AAGAGATGCTGAATATCTCTTTGTTTTATCGTACCTCGCAGCGTATTTCGTTAACGCCAGCAGGCAAAGCTTATTATATC
GATGTACTAAGTATTCTTAAGCATATCGAGACTGCGACGACCAATTTGATGTCGCATAGCAGTAATTTGGAGGTGCTAAA
AATGGTCTCGCATCCCACTTTTTGTGCACGTTGGCTGATTCCTGCGCTTCACGGCTTTAGTCAGGAAAACCCTTTAATTA
GGCTGGATATTAAAGAGCAGGTAGGTCCATTTTTCTCCGAAGAGCATGATATCGATATTGCGTTCTTATACGGAGAAGGG
ACGTGGTCGGGAATGCAAAACGTTAAATTGTTTGATGAGTACTGTGTGGCGGTTTGTCGCCCTGATTATTTGCAAGGTAA
AGACTTGCAACCGCAGCAGTCGGACAGCTATGTATTGCTACAGCTGAGTTCACGTCCTAGCGCATGGTACGAATACTTTA
AGCAGCAGCTGATTAGCTTGGATGGTACGTTTGTAGGCCCACGCTTTGATACGTTTCATGCCTGTATTTCGGCAGCTTTA
ACAGGTTATGGCGTTGCCTTGGTGCCGTTACGTTTGGTGGCTCCCGAGCTACAATCGGGTCAGCTGATGATGGCTTGGAA
ATATGCGGCCAAGGGGAGAGGGGCTTACTATATGTCTTATCCTTTATCGCTTGGCAACTCGCACAAGACCAAAGTGATGC
AAGAGTGGATTGCACGCTACTTAGAAACCTCTAGTCAGAGTCAGGTGGAGTTAGCCAGTTTGCTTGGTATCACTTGTACG
AGTATCAGCCACGGATAA

Upstream 100 bases:

>100_bases
GCTAGGCTGTAGTGGCATACTAACCTTAGACTGTGCTTAAAAGAAGTGATGTGATACGAAGTACCACATCTATTATCATG
GAAGAAAATAAAAACAGACT

Downstream 100 bases:

>100_bases
CGACAGTAAGATGATTAACTGCTAAAAGCTAATGATGAAGAATAACCTTTGCAATTAGCCACGATGACAAAAAGGAATGC
CCGCCCAACTATTATTCGTT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKTDTNPSLAFRRYPSTTALQCFETAARHLSFTNAALELHMTQSAISKQVAQLEEMLNISLFYRTSQRISLTPAGKAYYI
DVLSILKHIETATTNLMSHSSNLEVLKMVSHPTFCARWLIPALHGFSQENPLIRLDIKEQVGPFFSEEHDIDIAFLYGEG
TWSGMQNVKLFDEYCVAVCRPDYLQGKDLQPQQSDSYVLLQLSSRPSAWYEYFKQQLISLDGTFVGPRFDTFHACISAAL
TGYGVALVPLRLVAPELQSGQLMMAWKYAAKGRGAYYMSYPLSLGNSHKTKVMQEWIARYLETSSQSQVELASLLGITCT
SISHG

Sequences:

>Translated_325_residues
MKTDTNPSLAFRRYPSTTALQCFETAARHLSFTNAALELHMTQSAISKQVAQLEEMLNISLFYRTSQRISLTPAGKAYYI
DVLSILKHIETATTNLMSHSSNLEVLKMVSHPTFCARWLIPALHGFSQENPLIRLDIKEQVGPFFSEEHDIDIAFLYGEG
TWSGMQNVKLFDEYCVAVCRPDYLQGKDLQPQQSDSYVLLQLSSRPSAWYEYFKQQLISLDGTFVGPRFDTFHACISAAL
TGYGVALVPLRLVAPELQSGQLMMAWKYAAKGRGAYYMSYPLSLGNSHKTKVMQEWIARYLETSSQSQVELASLLGITCT
SISHG
>Mature_325_residues
MKTDTNPSLAFRRYPSTTALQCFETAARHLSFTNAALELHMTQSAISKQVAQLEEMLNISLFYRTSQRISLTPAGKAYYI
DVLSILKHIETATTNLMSHSSNLEVLKMVSHPTFCARWLIPALHGFSQENPLIRLDIKEQVGPFFSEEHDIDIAFLYGEG
TWSGMQNVKLFDEYCVAVCRPDYLQGKDLQPQQSDSYVLLQLSSRPSAWYEYFKQQLISLDGTFVGPRFDTFHACISAAL
TGYGVALVPLRLVAPELQSGQLMMAWKYAAKGRGAYYMSYPLSLGNSHKTKVMQEWIARYLETSSQSQVELASLLGITCT
SISHG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=288, Percent_Identity=32.9861111111111, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1786448, Length=249, Percent_Identity=29.718875502008, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788706, Length=254, Percent_Identity=29.5275590551181, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1786401, Length=170, Percent_Identity=27.6470588235294, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=271, Percent_Identity=25.0922509225092, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI145693105, Length=172, Percent_Identity=26.7441860465116, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36512; Mature: 36512

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTDTNPSLAFRRYPSTTALQCFETAARHLSFTNAALELHMTQSAISKQVAQLEEMLNIS
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHCEE
LFYRTSQRISLTPAGKAYYIDVLSILKHIETATTNLMSHSSNLEVLKMVSHPTFCARWLI
EEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHH
PALHGFSQENPLIRLDIKEQVGPFFSEEHDIDIAFLYGEGTWSGMQNVKLFDEYCVAVCR
HHHHCCCCCCCEEEEEEHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCC
PDYLQGKDLQPQQSDSYVLLQLSSRPSAWYEYFKQQLISLDGTFVGPRFDTFHACISAAL
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
TGYGVALVPLRLVAPELQSGQLMMAWKYAAKGRGAYYMSYPLSLGNSHKTKVMQEWIARY
HCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LETSSQSQVELASLLGITCTSISHG
HCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTDTNPSLAFRRYPSTTALQCFETAARHLSFTNAALELHMTQSAISKQVAQLEEMLNIS
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHHCEE
LFYRTSQRISLTPAGKAYYIDVLSILKHIETATTNLMSHSSNLEVLKMVSHPTFCARWLI
EEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHH
PALHGFSQENPLIRLDIKEQVGPFFSEEHDIDIAFLYGEGTWSGMQNVKLFDEYCVAVCR
HHHHCCCCCCCEEEEEEHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCC
PDYLQGKDLQPQQSDSYVLLQLSSRPSAWYEYFKQQLISLDGTFVGPRFDTFHACISAAL
CCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHH
TGYGVALVPLRLVAPELQSGQLMMAWKYAAKGRGAYYMSYPLSLGNSHKTKVMQEWIARY
HCCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHH
LETSSQSQVELASLLGITCTSISHG
HCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]