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The map label for this gene is nth [H]

Identifier: 93005679

GI number: 93005679

Start: 1007277

End: 1007972

Strand: Reverse

Name: nth [H]

Synonym: Pcryo_0851

Alternate gene names: 93005679

Gene position: 1007972-1007277 (Counterclockwise)

Preceding gene: 93005680

Following gene: 93005678

Centisome position: 32.94

GC content: 45.11

Gene sequence:

>696_bases
ATGAGTAAAACCGTCAAACATAAAACAGCTGATACTCCGCCATCGAGGCGCATGCCCAACCGTGATGTGCGTCCATTTTT
TGAGAAATTGGCGGCGACAATTGATGAGCCAGTGACTGAGCTCAATTATAAGAGTAACTTTGAGCTGCTTATCGCAGTTA
TATTATCAGCGCAGGCGACTGATGTCAGCGTCAATATTGCCACCAATCAGCTCTATCCGGTGGCGAATACGCCTGAGGCA
ATACTCGCTTTAGGCGAAGAAGGTTTGAAATCCTATATTAAGAATATCGGCTTGTTTAATGCCAAAGCCAAAAACGTCAT
CAAAACTTGCCGTGACTTGATTGAGAAATTTAATAGCACCGTCCCTGACAACCGTAAAGACTTAGAGTCTCTAGCGGGCG
TCGGGCGCAAGACGGCGAATGTAGTTCTAAATACAGCCTTTGGACAGCCGACGATGGCGGTCGATACCCATATCTTTCGA
GTGGGTAATCGTACGGGACTTGCCACTGGCAAAAACGTCTTAATCGTTGAGAAGAAACTGGTCGAACGTATCCCCGATGA
CTTTATCGTTGATGCTCATCATTATCTTATTTTGCACGGGCGCTATACTTGTCAGGCTCGTACCCCAAAATGCGGCGCTT
GTCCTGTTTATGACGAATGTATGTTTAAAGATAAAGCAGTATTTTTAGAGTTGTAA

Upstream 100 bases:

>100_bases
AACAAGCAGAATTGAATGAATTAAAAGCTGAGCTTGCACAACTTTAATAGCCAATATCTACTTTTGAATAACCAATGAAC
AAAATAATGGTAAAAATACC

Downstream 100 bases:

>100_bases
TGTGAGCAGATGATAATGATCGCAAAGCCTATCGATCAATATACTTATGCTAAATTATAATAATATCATGATTGAGCATT
ACTTATAATAGAACGAGTGC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSKTVKHKTADTPPSRRMPNRDVRPFFEKLAATIDEPVTELNYKSNFELLIAVILSAQATDVSVNIATNQLYPVANTPEA
ILALGEEGLKSYIKNIGLFNAKAKNVIKTCRDLIEKFNSTVPDNRKDLESLAGVGRKTANVVLNTAFGQPTMAVDTHIFR
VGNRTGLATGKNVLIVEKKLVERIPDDFIVDAHHYLILHGRYTCQARTPKCGACPVYDECMFKDKAVFLEL

Sequences:

>Translated_231_residues
MSKTVKHKTADTPPSRRMPNRDVRPFFEKLAATIDEPVTELNYKSNFELLIAVILSAQATDVSVNIATNQLYPVANTPEA
ILALGEEGLKSYIKNIGLFNAKAKNVIKTCRDLIEKFNSTVPDNRKDLESLAGVGRKTANVVLNTAFGQPTMAVDTHIFR
VGNRTGLATGKNVLIVEKKLVERIPDDFIVDAHHYLILHGRYTCQARTPKCGACPVYDECMFKDKAVFLEL
>Mature_230_residues
SKTVKHKTADTPPSRRMPNRDVRPFFEKLAATIDEPVTELNYKSNFELLIAVILSAQATDVSVNIATNQLYPVANTPEAI
LALGEEGLKSYIKNIGLFNAKAKNVIKTCRDLIEKFNSTVPDNRKDLESLAGVGRKTANVVLNTAFGQPTMAVDTHIFRV
GNRTGLATGKNVLIVEKKLVERIPDDFIVDAHHYLILHGRYTCQARTPKCGACPVYDECMFKDKAVFLEL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=204, Percent_Identity=28.4313725490196, Blast_Score=79, Evalue=3e-15,
Organism=Escherichia coli, GI1787920, Length=189, Percent_Identity=68.2539682539683, Blast_Score=270, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17554540, Length=169, Percent_Identity=31.9526627218935, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=29.9435028248588, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 25649; Mature: 25518

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTVKHKTADTPPSRRMPNRDVRPFFEKLAATIDEPVTELNYKSNFELLIAVILSAQAT
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
DVSVNIATNQLYPVANTPEAILALGEEGLKSYIKNIGLFNAKAKNVIKTCRDLIEKFNST
EEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
VPDNRKDLESLAGVGRKTANVVLNTAFGQPTMAVDTHIFRVGNRTGLATGKNVLIVEKKL
CCCCHHHHHHHHHCCCCHHHEEEECCCCCCCHHHHHEEEEECCCCCCCCCCEEEEEEHHH
VERIPDDFIVDAHHYLILHGRYTCQARTPKCGACPVYDECMFKDKAVFLEL
HHHCCCCEEECCCEEEEEECCEEEEECCCCCCCCCCHHHHHCCCCEEEEEC
>Mature Secondary Structure 
SKTVKHKTADTPPSRRMPNRDVRPFFEKLAATIDEPVTELNYKSNFELLIAVILSAQAT
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCC
DVSVNIATNQLYPVANTPEAILALGEEGLKSYIKNIGLFNAKAKNVIKTCRDLIEKFNST
EEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
VPDNRKDLESLAGVGRKTANVVLNTAFGQPTMAVDTHIFRVGNRTGLATGKNVLIVEKKL
CCCCHHHHHHHHHCCCCHHHEEEECCCCCCCHHHHHEEEEECCCCCCCCCCEEEEEEHHH
VERIPDDFIVDAHHYLILHGRYTCQARTPKCGACPVYDECMFKDKAVFLEL
HHHCCCCEEECCCEEEEEECCEEEEECCCCCCCCCCHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]