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The map label for this gene is metK [H]

Identifier: 93005678

GI number: 93005678

Start: 1005646

End: 1006812

Strand: Reverse

Name: metK [H]

Synonym: Pcryo_0850

Alternate gene names: 93005678

Gene position: 1006812-1005646 (Counterclockwise)

Preceding gene: 93005679

Following gene: 93005677

Centisome position: 32.9

GC content: 46.87

Gene sequence:

>1167_bases
ATGCGCGAATACAACTTATTTACCTCAGAATCTGTGAGCGAAGGTCATCCTGACAAAATGGCTGATCAAATCTCAGACGC
TATCCTTGATGCCATCTTACGGCAAGATTTACATGCACGCGTGGCATGTGAGACCCTAGTCAAAACTGGCGCTGTCGTAT
TGGCAGGTGAAATAACCACCACGGCAAATATCGACGTTGAGCGCATCGTGCGTGATACGGTTAATGGCATTGGTTATCAT
CATTCAGACTTGGGTTTTGATGGCGAGACATGTGCGGTTATTAATATGCTTGGTAAACAATCGCCTGAGATTGCCCAAGG
TGTTGACCGCGCTGACCCTGAAGAACAAGGCGCTGGTGACCAAGGTTTGATGTTTGGTTATGCTAGCAATGAAACTGAAG
TATTGATGCCCGCCCCTATCGAGTATGCTCATCGCTTGATGGAGCGTCAGTCGGAGTTGCGCCGCGCAGGCGAACTACCT
TGGTTACGTCCGGATGCCAAAGCACAAGTCACGCTTAAATATGATGGCAATAAACCTGTTGCTATTGACGCGGTCGTCTT
ATCTACGCAGCATGATCCAAGTATCTCGCAAGCTGATTTACAAGAAGCGATCATGGAGTCAATTATCAAACAAGTACTCC
CGGCAGAGCTACTACATGCTGGTACACGTTATCATATCAACCCGACTGGCAAATTCGTCATCGGTGGTCCTGTTGGTGAT
GCAGGACTGACCGGTCGTAAGATTATTGTTGATACTTATGGCGGTATGGCGCGTCACGGTGGCGGCGCATTTAGTGGTAA
AGATCCCTCAAAAGTCGATCGCAGTGCAGCTTATGCGGTGCGTTACGTGGCTAAAAACATCGTCGCTGCTGGTATTGCCG
ATCGCTGTGAAGTACAAGTAAGCTATGCCATTGGCGTTGCTGAACCGACTTCTATTTCGATTAACACTTTTGGTACTAAC
AAAATCAGCAACGATGAAATCATTAGCTTGATTCGTACGCATTTTGACTTACGTCCGTATGGTATCACCCGTATGCTGAA
TTTATTGCAGCCGATGTATCAGCAAACCGCCAGTTTTGGACATTTTGGTCGCCAAGGTTCTGAAACCGCCTTTACGTGGG
AAAAAACTGACAAAGCTAAGATTTTAAAAGCGGATGCTGGCTTATAA

Upstream 100 bases:

>100_bases
TAAACACCAAGCAATACATTGTTTGGCCAATACCAATTAAAGCAGCGCCCAGACGTTATGCTTTTTCCTATCTTTATTGA
TTCAATTAAGCGATCCTATT

Downstream 100 bases:

>100_bases
AATTTTTGCTAAAACCCTTTTTGTACCTATTATCTTATCCAGTTATTAGACTGCTGTGATACATTTAAATTTACCTACCA
TTATTAGGAGTCGTTTATGT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MREYNLFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVVLAGEITTTANIDVERIVRDTVNGIGYH
HSDLGFDGETCAVINMLGKQSPEIAQGVDRADPEEQGAGDQGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGELP
WLRPDAKAQVTLKYDGNKPVAIDAVVLSTQHDPSISQADLQEAIMESIIKQVLPAELLHAGTRYHINPTGKFVIGGPVGD
AGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAVRYVAKNIVAAGIADRCEVQVSYAIGVAEPTSISINTFGTN
KISNDEIISLIRTHFDLRPYGITRMLNLLQPMYQQTASFGHFGRQGSETAFTWEKTDKAKILKADAGL

Sequences:

>Translated_388_residues
MREYNLFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVVLAGEITTTANIDVERIVRDTVNGIGYH
HSDLGFDGETCAVINMLGKQSPEIAQGVDRADPEEQGAGDQGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGELP
WLRPDAKAQVTLKYDGNKPVAIDAVVLSTQHDPSISQADLQEAIMESIIKQVLPAELLHAGTRYHINPTGKFVIGGPVGD
AGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAVRYVAKNIVAAGIADRCEVQVSYAIGVAEPTSISINTFGTN
KISNDEIISLIRTHFDLRPYGITRMLNLLQPMYQQTASFGHFGRQGSETAFTWEKTDKAKILKADAGL
>Mature_388_residues
MREYNLFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVVLAGEITTTANIDVERIVRDTVNGIGYH
HSDLGFDGETCAVINMLGKQSPEIAQGVDRADPEEQGAGDQGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGELP
WLRPDAKAQVTLKYDGNKPVAIDAVVLSTQHDPSISQADLQEAIMESIIKQVLPAELLHAGTRYHINPTGKFVIGGPVGD
AGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAVRYVAKNIVAAGIADRCEVQVSYAIGVAEPTSISINTFGTN
KISNDEIISLIRTHFDLRPYGITRMLNLLQPMYQQTASFGHFGRQGSETAFTWEKTDKAKILKADAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=375, Percent_Identity=57.6, Blast_Score=414, Evalue=1e-116,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=56.4304461942257, Blast_Score=411, Evalue=1e-115,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=71.6145833333333, Blast_Score=585, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI17538498, Length=381, Percent_Identity=55.9055118110236, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17538494, Length=375, Percent_Identity=56, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25145633, Length=383, Percent_Identity=55.6135770234987, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=52.9562982005141, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=381, Percent_Identity=50.3937007874016, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32565911, Length=335, Percent_Identity=52.5373134328358, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=337, Percent_Identity=52.2255192878338, Blast_Score=337, Evalue=8e-93,
Organism=Caenorhabditis elegans, GI32565903, Length=309, Percent_Identity=52.4271844660194, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=61.4457831325301, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=75, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=378, Percent_Identity=55.026455026455, Blast_Score=403, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=54.3307086614173, Blast_Score=395, Evalue=1e-111,
Organism=Drosophila melanogaster, GI45552159, Length=386, Percent_Identity=57.5129533678757, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580529, Length=386, Percent_Identity=57.5129533678757, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580537, Length=386, Percent_Identity=57.5129533678757, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580533, Length=386, Percent_Identity=57.5129533678757, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580535, Length=386, Percent_Identity=57.5129533678757, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580531, Length=386, Percent_Identity=57.5129533678757, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580527, Length=385, Percent_Identity=55.8441558441558, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=385, Percent_Identity=55.8441558441558, Blast_Score=395, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=387, Percent_Identity=53.4883720930233, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=58.1196581196581, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42053; Mature: 42053

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYNLFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVVLAGEITT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEE
TANIDVERIVRDTVNGIGYHHSDLGFDGETCAVINMLGKQSPEIAQGVDRADPEEQGAGD
ECCCHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCHHHCCCC
QGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGELPWLRPDAKAQVTLKYDGNKPV
CCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCE
AIDAVVLSTQHDPSISQADLQEAIMESIIKQVLPAELLHAGTRYHINPTGKFVIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCC
AGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAVRYVAKNIVAAGIADRCEVQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SYAIGVAEPTSISINTFGTNKISNDEIISLIRTHFDLRPYGITRMLNLLQPMYQQTASFG
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
HFGRQGSETAFTWEKTDKAKILKADAGL
HCCCCCCCCEEEECCCCCCEEEECCCCC
>Mature Secondary Structure
MREYNLFTSESVSEGHPDKMADQISDAILDAILRQDLHARVACETLVKTGAVVLAGEITT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEE
TANIDVERIVRDTVNGIGYHHSDLGFDGETCAVINMLGKQSPEIAQGVDRADPEEQGAGD
ECCCHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCHHHCCCC
QGLMFGYASNETEVLMPAPIEYAHRLMERQSELRRAGELPWLRPDAKAQVTLKYDGNKPV
CCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCE
AIDAVVLSTQHDPSISQADLQEAIMESIIKQVLPAELLHAGTRYHINPTGKFVIGGPVGD
EEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEECCCCC
AGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAVRYVAKNIVAAGIADRCEVQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
SYAIGVAEPTSISINTFGTNKISNDEIISLIRTHFDLRPYGITRMLNLLQPMYQQTASFG
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
HFGRQGSETAFTWEKTDKAKILKADAGL
HCCCCCCCCEEEECCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA