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The map label for this gene is clpB [H]

Identifier: 93005671

GI number: 93005671

Start: 994955

End: 997552

Strand: Direct

Name: clpB [H]

Synonym: Pcryo_0841

Alternate gene names: 93005671

Gene position: 994955-997552 (Clockwise)

Preceding gene: 93005670

Following gene: 93005672

Centisome position: 32.52

GC content: 46.11

Gene sequence:

>2598_bases
ATGAGATTCGAGAAGTTTACCCAAAAGTTGCAATCTGCCATTCAAGAAGCACAAAGTCTGGCGTTAGGGCGCGACCATAC
GGGAATAGACCCTGTGCATTTGCTAGCAGTCTTGCTACAAGATGAGTCTAATTTGTCTATTTGCCAGCAAGCGGGCGCCT
CTATTCCTGCGCTAAAAAATGGCGTGGCAAAAGCGCTCGACAACCAAGCAACGATTAGTGAGCCAACAGGAGACGTTAAT
CTAAACCCAAATTCAGTCAAAATTCTGAACTTGGCAGACCGCCAAGCACAAAAAGAAGGTGATGATTTTATCGCCACCGA
ATGGGTAATGTTGGCACTGGCTGAACAAGGCGATACCAAAAAGGTATTTGAAAGTGCGGGTGTTACAGCCAATAAATTGA
AAGCCGTGATTGAGCAATTACGCGGTGATGATACCGTGAATAATCAAAACGCTGAAGATCAGCGTGATGCCCTCAATAAA
TATACGATTAACTTAACGGAGCAAGCAGAGCTAGGTAAGCTTGATCCTGTGATTGGTCGTGACGAAGAGATTCGCCGTAC
CATTCAGGTGTTGTCGCGCCGTACCAAAAATAACCCTGTGCTTATTGGTGAGCCGGGTGTTGGTAAAACGGCCATTGTAG
AAGGTTTGGCTCAAAAAATTATTAATGGTGATGTACCAGAAGGTTTACGTCGCAAAGAAGTGCTGTCGTTGGACTTAGGT
GCGTTAATTGCGGGGGCAAAATTCCGTGGTGAGTTCGAAGAGCGACTTAAAGCGGTACTTAGTGACTTATCGAAGCAAGA
AGGTAACGTTATCCTATTCATCGATGAGTTGCATACTTTAGTAGGTGCTGGTAAAGCAGATGGCGCGATGGACGCTGGTA
ATATGCTCAAACCAGCGCTTGCTCGCGGTGAGCTGCACTGCGTCGGTGCGACGACTTTAGATGAATATCGTAAGTATATT
GAAAAAGATGCAGCACTTGAGCGTCGTTTCCAAAAAGTATTGGTTGATGAGCCAACGGTCGAAGACACCATTGCGATATT
GCGTGGTCTAAAAGAGCGCTATGAGCTTCATCATGGAGTCGATATCCAAGACAGCGCCATCATTGCTGCTGCGCGGATGA
GCCATCGTTATATTACTGATCGCAAGTTACCGGATAAGGCGATTGACTTGATTGATGAAGCCGCCAGTCGCTTACGTATG
GAGATGGACAGCAAACCTGAATCTTTAGGTAAGCTCGATAGCCGTCTGATACAGCTAAAAATGCAGCGCGAAGTACTCAA
AAATGAAGAAGATGCTGGTGCGCAAGCAGAGCGTAAAGTGCTTGATGCACAAATTGAAGAGTTAGAGAAAGAATACGCTG
ATCTCAACGAGATCTGGCAAGCAGAAAAAGCTTTGGTCAAAGGCAGTCAGCAGCTAAAAGACGAGCTGGACAAAGCGCGT
ATTGCTTATGATAAAGCCAGTCGTGAAGGCGATTATGAGATCATGAGTAAGCTACAATACGAGACGATTCCGCAGTTAGA
GCGCCGTATCCATGAGTCTGACCTGGCAGAGCAAAAAGAGCAGACTGGTGAAAATAGTGGTGTAAAACTGCTGCGTAACA
AAGTCACGGATAATGAAATTGCTGAAGTCGTTGCTGCAGCGACGGGTATTCCAGTCAGTAAAATGATGCAAGGCGAGCGT
GATAAGATGCTGGCAATGGAAGAAAAACTCCATGAGCGCGTCATCGGTCAAGACGAAGCAGTTGAAGCGGTAGCCAATGC
GGTACGCCGTAGCCGTGCTGGCTTGTCTGATCCGAATCGTCCTTCAGGTTCATTCTTATTCTTAGGACCTACGGGTGTCG
GTAAGACTGAGCTGACCAAGTCATTGGCGAACTTCTTGTTCGATAGTGAAGATGCTATTGTACGTATCGATATGAGTGAG
TATATGGAGAAGCACAGCGTCAGCCGCTTGGTTGGTGCCCCGCCAGGCTACGTCGGTTATGAAGAAGGTGGTGCCTTGAC
TGAAGCGGTACGCCGTAAACCTTATAGTGTGATTTTGTTTGATGAAGTTGAAAAAGCGCATCCTGATGTGTTTAATATCT
TGCTACAAGTGTTGGATGATGGTCGTTTGACTGACTCACAAGGTCGTGTCGTTGATTTCAAGAATACTGTGATTATTATG
ACCAGTAACTTGGGTTCGCATAAAATCCAAGAAATGGTCGGCGAGAGCTATGAGGATATCAAAGCTGAAGTGATGGATTC
AGTTGGGCAGCATTTCCGTCCAGAGTTTGTCAACCGTATCGATGAGATCGTGGTATTCCATCCACTAGGTATGTCGCAGA
TGGCAGGTATTGCTGCGATTCAGTTAAACCGCTTGCGTCAGCGTTTATATGAGCGTGAGTTAGATATCGAGTTATCAGCA
GAAGCGATGAATAAGCTGGTTGCGGTTGGTTATGATCCTGTTTATGGCGCGCGTCCACTCAAGCGTGCCATTCAGCAAGA
GATTGAAAACCCATTGTCATTGAAGCTGCTGGCAGGTGACTTTGTTGCTGGTGATATCATCAAAGTCGATGTCGGTGTGG
ATGATAAGCTGACATTTGATAAGCTTAGAATGAGCTAA

Upstream 100 bases:

>100_bases
GATATAGATAACAACGAAAAGCTTAAGGCATCTTAATACTTATAAAAAGCCGCAGCTACTATCAGATATAAAATATTATT
AATTATAAGAGGAAACAAAT

Downstream 100 bases:

>100_bases
TCGTTCATTTTAGCCCTTGACCCGAAACTAAGCCCCTTATTTGCCCATTTTTTATACCGGCAAGTAAGGGGTTTTTACTT
TGTGCGCCATCATTATTTTT

Product: ATPase AAA-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 865; Mature: 865

Protein sequence:

>865_residues
MRFEKFTQKLQSAIQEAQSLALGRDHTGIDPVHLLAVLLQDESNLSICQQAGASIPALKNGVAKALDNQATISEPTGDVN
LNPNSVKILNLADRQAQKEGDDFIATEWVMLALAEQGDTKKVFESAGVTANKLKAVIEQLRGDDTVNNQNAEDQRDALNK
YTINLTEQAELGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQKIINGDVPEGLRRKEVLSLDLG
ALIAGAKFRGEFEERLKAVLSDLSKQEGNVILFIDELHTLVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRKYI
EKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHGVDIQDSAIIAAARMSHRYITDRKLPDKAIDLIDEAASRLRM
EMDSKPESLGKLDSRLIQLKMQREVLKNEEDAGAQAERKVLDAQIEELEKEYADLNEIWQAEKALVKGSQQLKDELDKAR
IAYDKASREGDYEIMSKLQYETIPQLERRIHESDLAEQKEQTGENSGVKLLRNKVTDNEIAEVVAAATGIPVSKMMQGER
DKMLAMEEKLHERVIGQDEAVEAVANAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELTKSLANFLFDSEDAIVRIDMSE
YMEKHSVSRLVGAPPGYVGYEEGGALTEAVRRKPYSVILFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVVDFKNTVIIM
TSNLGSHKIQEMVGESYEDIKAEVMDSVGQHFRPEFVNRIDEIVVFHPLGMSQMAGIAAIQLNRLRQRLYERELDIELSA
EAMNKLVAVGYDPVYGARPLKRAIQQEIENPLSLKLLAGDFVAGDIIKVDVGVDDKLTFDKLRMS

Sequences:

>Translated_865_residues
MRFEKFTQKLQSAIQEAQSLALGRDHTGIDPVHLLAVLLQDESNLSICQQAGASIPALKNGVAKALDNQATISEPTGDVN
LNPNSVKILNLADRQAQKEGDDFIATEWVMLALAEQGDTKKVFESAGVTANKLKAVIEQLRGDDTVNNQNAEDQRDALNK
YTINLTEQAELGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQKIINGDVPEGLRRKEVLSLDLG
ALIAGAKFRGEFEERLKAVLSDLSKQEGNVILFIDELHTLVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRKYI
EKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHGVDIQDSAIIAAARMSHRYITDRKLPDKAIDLIDEAASRLRM
EMDSKPESLGKLDSRLIQLKMQREVLKNEEDAGAQAERKVLDAQIEELEKEYADLNEIWQAEKALVKGSQQLKDELDKAR
IAYDKASREGDYEIMSKLQYETIPQLERRIHESDLAEQKEQTGENSGVKLLRNKVTDNEIAEVVAAATGIPVSKMMQGER
DKMLAMEEKLHERVIGQDEAVEAVANAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELTKSLANFLFDSEDAIVRIDMSE
YMEKHSVSRLVGAPPGYVGYEEGGALTEAVRRKPYSVILFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVVDFKNTVIIM
TSNLGSHKIQEMVGESYEDIKAEVMDSVGQHFRPEFVNRIDEIVVFHPLGMSQMAGIAAIQLNRLRQRLYERELDIELSA
EAMNKLVAVGYDPVYGARPLKRAIQQEIENPLSLKLLAGDFVAGDIIKVDVGVDDKLTFDKLRMS
>Mature_865_residues
MRFEKFTQKLQSAIQEAQSLALGRDHTGIDPVHLLAVLLQDESNLSICQQAGASIPALKNGVAKALDNQATISEPTGDVN
LNPNSVKILNLADRQAQKEGDDFIATEWVMLALAEQGDTKKVFESAGVTANKLKAVIEQLRGDDTVNNQNAEDQRDALNK
YTINLTEQAELGKLDPVIGRDEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQKIINGDVPEGLRRKEVLSLDLG
ALIAGAKFRGEFEERLKAVLSDLSKQEGNVILFIDELHTLVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRKYI
EKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHGVDIQDSAIIAAARMSHRYITDRKLPDKAIDLIDEAASRLRM
EMDSKPESLGKLDSRLIQLKMQREVLKNEEDAGAQAERKVLDAQIEELEKEYADLNEIWQAEKALVKGSQQLKDELDKAR
IAYDKASREGDYEIMSKLQYETIPQLERRIHESDLAEQKEQTGENSGVKLLRNKVTDNEIAEVVAAATGIPVSKMMQGER
DKMLAMEEKLHERVIGQDEAVEAVANAVRRSRAGLSDPNRPSGSFLFLGPTGVGKTELTKSLANFLFDSEDAIVRIDMSE
YMEKHSVSRLVGAPPGYVGYEEGGALTEAVRRKPYSVILFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVVDFKNTVIIM
TSNLGSHKIQEMVGESYEDIKAEVMDSVGQHFRPEFVNRIDEIVVFHPLGMSQMAGIAAIQLNRLRQRLYERELDIELSA
EAMNKLVAVGYDPVYGARPLKRAIQQEIENPLSLKLLAGDFVAGDIIKVDVGVDDKLTFDKLRMS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=323, Percent_Identity=35.9133126934984, Blast_Score=211, Evalue=2e-54,
Organism=Escherichia coli, GI1788943, Length=858, Percent_Identity=65.034965034965, Blast_Score=1122, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=416, Percent_Identity=38.2211538461538, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=707, Percent_Identity=52.8995756718529, Blast_Score=749, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=868, Percent_Identity=42.0506912442396, Blast_Score=659, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95982; Mature: 95982

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFEKFTQKLQSAIQEAQSLALGRDHTGIDPVHLLAVLLQDESNLSICQQAGASIPALKN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
GVAKALDNQATISEPTGDVNLNPNSVKILNLADRQAQKEGDDFIATEWVMLALAEQGDTK
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
KVFESAGVTANKLKAVIEQLRGDDTVNNQNAEDQRDALNKYTINLTEQAELGKLDPVIGR
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCC
DEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQKIINGDVPEGLRRKEVLSLDLG
CHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALIAGAKFRGEFEERLKAVLSDLSKQEGNVILFIDELHTLVGAGKADGAMDAGNMLKPAL
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHH
ARGELHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHGV
HCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DIQDSAIIAAARMSHRYITDRKLPDKAIDLIDEAASRLRMEMDSKPESLGKLDSRLIQLK
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MQREVLKNEEDAGAQAERKVLDAQIEELEKEYADLNEIWQAEKALVKGSQQLKDELDKAR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
IAYDKASREGDYEIMSKLQYETIPQLERRIHESDLAEQKEQTGENSGVKLLRNKVTDNEI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
AEVVAAATGIPVSKMMQGERDKMLAMEEKLHERVIGQDEAVEAVANAVRRSRAGLSDPNR
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
PSGSFLFLGPTGVGKTELTKSLANFLFDSEDAIVRIDMSEYMEKHSVSRLVGAPPGYVGY
CCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCC
EEGGALTEAVRRKPYSVILFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVVDFKNTVIIM
CCCCHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEE
TSNLGSHKIQEMVGESYEDIKAEVMDSVGQHFRPEFVNRIDEIVVFHPLGMSQMAGIAAI
ECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHH
QLNRLRQRLYERELDIELSAEAMNKLVAVGYDPVYGARPLKRAIQQEIENPLSLKLLAGD
HHHHHHHHHHHHHCCCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
FVAGDIIKVDVGVDDKLTFDKLRMS
EEECCEEEEECCCCCCCCHHHHCCC
>Mature Secondary Structure
MRFEKFTQKLQSAIQEAQSLALGRDHTGIDPVHLLAVLLQDESNLSICQQAGASIPALKN
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHCCCCCCHHHH
GVAKALDNQATISEPTGDVNLNPNSVKILNLADRQAQKEGDDFIATEWVMLALAEQGDTK
HHHHHHCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHCCCCHHHHHHHHHHHHCCCCHH
KVFESAGVTANKLKAVIEQLRGDDTVNNQNAEDQRDALNKYTINLTEQAELGKLDPVIGR
HHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCC
DEEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQKIINGDVPEGLRRKEVLSLDLG
CHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
ALIAGAKFRGEFEERLKAVLSDLSKQEGNVILFIDELHTLVGAGKADGAMDAGNMLKPAL
HHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHH
ARGELHCVGATTLDEYRKYIEKDAALERRFQKVLVDEPTVEDTIAILRGLKERYELHHGV
HCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
DIQDSAIIAAARMSHRYITDRKLPDKAIDLIDEAASRLRMEMDSKPESLGKLDSRLIQLK
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
MQREVLKNEEDAGAQAERKVLDAQIEELEKEYADLNEIWQAEKALVKGSQQLKDELDKAR
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
IAYDKASREGDYEIMSKLQYETIPQLERRIHESDLAEQKEQTGENSGVKLLRNKVTDNEI
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHHH
AEVVAAATGIPVSKMMQGERDKMLAMEEKLHERVIGQDEAVEAVANAVRRSRAGLSDPNR
HHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCC
PSGSFLFLGPTGVGKTELTKSLANFLFDSEDAIVRIDMSEYMEKHSVSRLVGAPPGYVGY
CCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCC
EEGGALTEAVRRKPYSVILFDEVEKAHPDVFNILLQVLDDGRLTDSQGRVVDFKNTVIIM
CCCCHHHHHHHCCCCEEEEECCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEE
TSNLGSHKIQEMVGESYEDIKAEVMDSVGQHFRPEFVNRIDEIVVFHPLGMSQMAGIAAI
ECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEEECCCCHHHHHHHHHH
QLNRLRQRLYERELDIELSAEAMNKLVAVGYDPVYGARPLKRAIQQEIENPLSLKLLAGD
HHHHHHHHHHHHHCCCEECHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
FVAGDIIKVDVGVDDKLTFDKLRMS
EEECCEEEEECCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]