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The map label for this gene is dppA [H]

Identifier: 93005589

GI number: 93005589

Start: 892578

End: 894221

Strand: Reverse

Name: dppA [H]

Synonym: Pcryo_0759

Alternate gene names: 93005589

Gene position: 894221-892578 (Counterclockwise)

Preceding gene: 93005590

Following gene: 93005588

Centisome position: 29.22

GC content: 45.01

Gene sequence:

>1644_bases
GTGCAAACTCCATTTTTCAAGCTCACCTTATTAGCAGCCTTAACGCTAGGCGTCAGCGCCTGTAGCGGCGACAATAAAAC
TGCGGATAAAACGGCAGATGCCGGTAGTGATGCCAAAGCCGCTAAGACCTTAATTTACTGTTCAGAAGGTAGCCCTGCCG
GCTTTGACCCTGCGCAATATACGGCAGGTACTGACTTTGATGCCAGTGCTTATCCTATTTTTAATGGTATCGTACAGTTT
AAACGTGGCGAAACCGAAATCGAGCCAGGCTTAGCCGAAAGCTGGGAAATCAGCGAAGACGGTAAGACTTATACGCTCAA
TCTACGTAAAGGCGTCAAATTCGGCAAGACCGATTATTTCACCCCAACTCGTGACTTCAATGCTGATGACATTATCTTTA
CCATCGAACGTCTTACCGATACAGGCTTTGCTTTTAATAAAGACTATCCAGCTGAATTCCCATATTCAGTAGGCATGGGC
TTACCTGATATTATCGACAAAGTAGAAAAAGTCGATGATTATACGGTCAAAATCACTCTGACTGAGACCAACGCGCCATT
CTTACAAAACCTAGCAATGCCGTTCGCCTATGTGCATTCTGCAGAGTATGCCAAGCAGTTACAAGACGCTGGCAATGCAG
CTGATCTAAATACCAAACCAGTCGGTACCGGACCTTTCGTCTTTACTTCGTATCAAAAAGATGCGCAGATTCGTTATACC
AAAAATCCTGACTACTGGAACAAAGACAACATTCATATCGATAACCTCGTCTTTGTCATCACCAAAGACTCAGCGGTTCG
TGCCCAAAAAGTACAAGCAGGCGAATGTAATGTCTCGGCTTACGCTAATCCATCTGAAGTGGACTCTGCCAAAAAATCTG
GCAAAGCAAACGTACTAGATGATGCAGGCTTTAACGTCGGTTATTTAGGCTATAATACTGAAAAACCCGATCTAAAAGAT
GTCAATGTTCGCCGCGCTCTCGATATGGCAATCAATAAAGACGCCATCATCAATGCGGTATATCAAGGTTCTGGTATCAA
AGCAAGCAACCCAATGCCACCAACGCAGTGGGGCTATAACGATGAGATCAAAGATGCGCCTTACGATATCGAAAAAGCCA
AAGCCCTACTGGCTGAAGCGGGTGCTAAAGATTTAGAAATCGACTTATGGTATATGCCCGTTCAGCGTCCTTATAATCCC
AATGCTAAGCTCATGGCAGAAATGATTCAATCTGATTGGGCTAAAATTGGGGTCAAAGCCAAGCTCGTGACTTATGAGTG
GGGCGAGTATCTAAAGCGTGCTGGCAAAGGTGAACCTGAAGCGATCCTAGCCGGTTGGACAGGCGATAATGGCGATCCTG
ATAACTGGTTAGGTGTTTTACTATCTTGTGATGCCGTCAATGGTAACAACTACTCGCGCTGGTGTAATGCAGAGTTTGAT
AAGCTGGTCATGGATGCGCGTGCAAATACCAACCAAGATGAGCGTATTGCTAATTACAAGCAGGCTCAGGTTATCTTTAA
AGACCAATTGCCTTGGACCACAGTTGCCAACTCAGTTGTGACGGTATTGACCACCCCTAATGTGAAAGACTACAAAATTA
GCCCACTCGGCTCCATTCGCTTTGATGGCGTTGATATTGAATAA

Upstream 100 bases:

>100_bases
TAAAGGTTTATCGATTAGAGTTCGATATCAACGGCGTCTAACGTCGTTGTCGTGCTATCAATCGATAATTATCTAACGAT
TTATACAGGATGTAATCACA

Downstream 100 bases:

>100_bases
AGCTATTAAATAGCATTAGCTAATACCAGTGCTACATTCGGTACTGGTATTAGATTCAATACCAGTACCTAACAAACCCT
AGTGACGCCTTTTACTTTAT

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 547; Mature: 547

Protein sequence:

>547_residues
MQTPFFKLTLLAALTLGVSACSGDNKTADKTADAGSDAKAAKTLIYCSEGSPAGFDPAQYTAGTDFDASAYPIFNGIVQF
KRGETEIEPGLAESWEISEDGKTYTLNLRKGVKFGKTDYFTPTRDFNADDIIFTIERLTDTGFAFNKDYPAEFPYSVGMG
LPDIIDKVEKVDDYTVKITLTETNAPFLQNLAMPFAYVHSAEYAKQLQDAGNAADLNTKPVGTGPFVFTSYQKDAQIRYT
KNPDYWNKDNIHIDNLVFVITKDSAVRAQKVQAGECNVSAYANPSEVDSAKKSGKANVLDDAGFNVGYLGYNTEKPDLKD
VNVRRALDMAINKDAIINAVYQGSGIKASNPMPPTQWGYNDEIKDAPYDIEKAKALLAEAGAKDLEIDLWYMPVQRPYNP
NAKLMAEMIQSDWAKIGVKAKLVTYEWGEYLKRAGKGEPEAILAGWTGDNGDPDNWLGVLLSCDAVNGNNYSRWCNAEFD
KLVMDARANTNQDERIANYKQAQVIFKDQLPWTTVANSVVTVLTTPNVKDYKISPLGSIRFDGVDIE

Sequences:

>Translated_547_residues
MQTPFFKLTLLAALTLGVSACSGDNKTADKTADAGSDAKAAKTLIYCSEGSPAGFDPAQYTAGTDFDASAYPIFNGIVQF
KRGETEIEPGLAESWEISEDGKTYTLNLRKGVKFGKTDYFTPTRDFNADDIIFTIERLTDTGFAFNKDYPAEFPYSVGMG
LPDIIDKVEKVDDYTVKITLTETNAPFLQNLAMPFAYVHSAEYAKQLQDAGNAADLNTKPVGTGPFVFTSYQKDAQIRYT
KNPDYWNKDNIHIDNLVFVITKDSAVRAQKVQAGECNVSAYANPSEVDSAKKSGKANVLDDAGFNVGYLGYNTEKPDLKD
VNVRRALDMAINKDAIINAVYQGSGIKASNPMPPTQWGYNDEIKDAPYDIEKAKALLAEAGAKDLEIDLWYMPVQRPYNP
NAKLMAEMIQSDWAKIGVKAKLVTYEWGEYLKRAGKGEPEAILAGWTGDNGDPDNWLGVLLSCDAVNGNNYSRWCNAEFD
KLVMDARANTNQDERIANYKQAQVIFKDQLPWTTVANSVVTVLTTPNVKDYKISPLGSIRFDGVDIE
>Mature_547_residues
MQTPFFKLTLLAALTLGVSACSGDNKTADKTADAGSDAKAAKTLIYCSEGSPAGFDPAQYTAGTDFDASAYPIFNGIVQF
KRGETEIEPGLAESWEISEDGKTYTLNLRKGVKFGKTDYFTPTRDFNADDIIFTIERLTDTGFAFNKDYPAEFPYSVGMG
LPDIIDKVEKVDDYTVKITLTETNAPFLQNLAMPFAYVHSAEYAKQLQDAGNAADLNTKPVGTGPFVFTSYQKDAQIRYT
KNPDYWNKDNIHIDNLVFVITKDSAVRAQKVQAGECNVSAYANPSEVDSAKKSGKANVLDDAGFNVGYLGYNTEKPDLKD
VNVRRALDMAINKDAIINAVYQGSGIKASNPMPPTQWGYNDEIKDAPYDIEKAKALLAEAGAKDLEIDLWYMPVQRPYNP
NAKLMAEMIQSDWAKIGVKAKLVTYEWGEYLKRAGKGEPEAILAGWTGDNGDPDNWLGVLLSCDAVNGNNYSRWCNAEFD
KLVMDARANTNQDERIANYKQAQVIFKDQLPWTTVANSVVTVLTTPNVKDYKISPLGSIRFDGVDIE

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=509, Percent_Identity=53.4381139489195, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1787551, Length=545, Percent_Identity=31.1926605504587, Blast_Score=294, Evalue=1e-80,
Organism=Escherichia coli, GI1787762, Length=501, Percent_Identity=27.9441117764471, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1787052, Length=528, Percent_Identity=26.1363636363636, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787495, Length=522, Percent_Identity=25.6704980842912, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI1789397, Length=507, Percent_Identity=26.232741617357, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI87081878, Length=525, Percent_Identity=25.5238095238095, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789887, Length=512, Percent_Identity=23.828125, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60290; Mature: 60290

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPFFKLTLLAALTLGVSACSGDNKTADKTADAGSDAKAAKTLIYCSEGSPAGFDPAQY
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHC
TAGTDFDASAYPIFNGIVQFKRGETEIEPGLAESWEISEDGKTYTLNLRKGVKFGKTDYF
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCCCC
TPTRDFNADDIIFTIERLTDTGFAFNKDYPAEFPYSVGMGLPDIIDKVEKVDDYTVKITL
CCCCCCCCCCEEEEEEHHCCCCCEECCCCCCCCCHHHCCCCHHHHHHHHHCCCEEEEEEE
TETNAPFLQNLAMPFAYVHSAEYAKQLQDAGNAADLNTKPVGTGPFVFTSYQKDAQIRYT
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEE
KNPDYWNKDNIHIDNLVFVITKDSAVRAQKVQAGECNVSAYANPSEVDSAKKSGKANVLD
CCCCCCCCCCEEECCEEEEEECCCCHHHHHCCCCCCCEEECCCHHHHHHHHHCCCCCEEC
DAGFNVGYLGYNTEKPDLKDVNVRRALDMAINKDAIINAVYQGSGIKASNPMPPTQWGYN
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCCCC
DEIKDAPYDIEKAKALLAEAGAKDLEIDLWYMPVQRPYNPNAKLMAEMIQSDWAKIGVKA
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEE
KLVTYEWGEYLKRAGKGEPEAILAGWTGDNGDPDNWLGVLLSCDAVNGNNYSRWCNAEFD
EEEEEHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHEEEEEEEEECCCCCCCEECCCCHH
KLVMDARANTNQDERIANYKQAQVIFKDQLPWTTVANSVVTVLTTPNVKDYKISPLGSIR
HHHHHCCCCCCHHHHHHCCHHEEEEEECCCCHHHHCCCEEEEEECCCCCCEEECCCCCEE
FDGVDIE
ECCCCCC
>Mature Secondary Structure
MQTPFFKLTLLAALTLGVSACSGDNKTADKTADAGSDAKAAKTLIYCSEGSPAGFDPAQY
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHC
TAGTDFDASAYPIFNGIVQFKRGETEIEPGLAESWEISEDGKTYTLNLRKGVKFGKTDYF
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCEEEEEECCCCCCCCCCCC
TPTRDFNADDIIFTIERLTDTGFAFNKDYPAEFPYSVGMGLPDIIDKVEKVDDYTVKITL
CCCCCCCCCCEEEEEEHHCCCCCEECCCCCCCCCHHHCCCCHHHHHHHHHCCCEEEEEEE
TETNAPFLQNLAMPFAYVHSAEYAKQLQDAGNAADLNTKPVGTGPFVFTSYQKDAQIRYT
EECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCEEEEE
KNPDYWNKDNIHIDNLVFVITKDSAVRAQKVQAGECNVSAYANPSEVDSAKKSGKANVLD
CCCCCCCCCCEEECCEEEEEECCCCHHHHHCCCCCCCEEECCCHHHHHHHHHCCCCCEEC
DAGFNVGYLGYNTEKPDLKDVNVRRALDMAINKDAIINAVYQGSGIKASNPMPPTQWGYN
CCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCHHEEEEECCCCCCCCCCCCCCCCCCC
DEIKDAPYDIEKAKALLAEAGAKDLEIDLWYMPVQRPYNPNAKLMAEMIQSDWAKIGVKA
CCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEE
KLVTYEWGEYLKRAGKGEPEAILAGWTGDNGDPDNWLGVLLSCDAVNGNNYSRWCNAEFD
EEEEEHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHEEEEEEEEECCCCCCCEECCCCHH
KLVMDARANTNQDERIANYKQAQVIFKDQLPWTTVANSVVTVLTTPNVKDYKISPLGSIR
HHHHHCCCCCCHHHHHHCCHHEEEEEECCCCHHHHCCCEEEEEECCCCCCEEECCCCCEE
FDGVDIE
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]