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The map label for this gene is dapE [H]

Identifier: 93005379

GI number: 93005379

Start: 646462

End: 647685

Strand: Reverse

Name: dapE [H]

Synonym: Pcryo_0549

Alternate gene names: 93005379

Gene position: 647685-646462 (Counterclockwise)

Preceding gene: 93005380

Following gene: 93005372

Centisome position: 21.17

GC content: 45.18

Gene sequence:

>1224_bases
ATGTCAGATTTTGATAAAAACCGAAATACTCAGTCTCAGACGCAAGCAACTCATCAACAAGCAACCTTGGACTTAAGCAT
CGCCCTACTTGAACGTCCATCAGTGACGCCAGATGATGACGGCTGCCAAGACATATTGTCTGAGCGGCTAACGCAAGCAG
GCTTCGACTGCGAGTTTATGTATTACGGTGATAGACAAGCAAAAGGCGAACACGCTGAGGTTAAAAACTTATGGGCGCGC
CGCGGTAATACCAATCCAGTCATTTGCTTTGCAGGTCATACTGACGTCGTCCCAACAGGCGATGAAAAAAATTGGACCTA
TCCGCCCTTTACCCCAACCATTGCAGATGGCTATTTATGGGCGCGTGGTGCCGCTGATATGAAAACTGGTATTGCCGCTT
TTACCGTCGCTGCTGAGCGTTTCGTTGCCAATCATCCTGAGCACAATGGCTCTATTGCTTTTTTAATCACCTCAGATGAA
GAAGGCCCTTCTATTAACGGCACAGTCAAGGTAGTAGAAACGCTAGAAGCGCGCAATGAGAAAATTACGTACTGCTTGGT
TGGTGAACCATCAAGCACGGATACATTAGGCGATATCATCAAGAATGGTCGTCGTGGCTCACTCGGCGCAGTACTTACCG
TCACTGGTAAACAAGGTCATGTTGCCTACCCGCACCTTGCCTCAAATCCCATTCATGCGGCTATGTCGGCACTCGCAGAA
CTGACCAATGCTACTTGGGATAATGGCAATGATTACTTCCCTGCGACTTCACTACAAATATCTAATATCAATAGTGGAAC
GGGCGCAACCAACGTCATTCCTGAAACCTTAGAAGCTGTATTTAACTTCCGCTTTTCAACAGAAACGTCTGAAGATGAAC
TAAAAGCAAAAACCCACGCTATCTTTGATAGATATTTCGCTGATAGCAAAGCAGCTTATGATATTCATTGGAAATTATCT
GGTCAGCCTTTTTTGACGCCTGAAGGTAAATTGGTCTCAGCATGCCAGCAAGCGATTAAATCTGTGACTGGAACCGATAC
GACATTATCAACTTCTGGCGGCACATCAGATGGACGCTTTATTGCACCAACGGGTGCGCAAGTGGTCGAACTTGGTGTGC
GTAATGCCACTATTCATCAAGTTGATGAAAAAGTTGAAGTAGACGACTTAGGAAAATTGGCGCAGATTTATGAAGGGATT
TTAGAGAATTTACTATTAGATTAA

Upstream 100 bases:

>100_bases
TATTGAAGACGTTATTTAAGTACTGTAATATTCATAAAGACTCTCTTACCATCTACATCTAAGCATACTATCTATCCCTC
TATAATAAAAAAGGCTTCCC

Downstream 100 bases:

>100_bases
TAAGCACTAGTAAAAAAAGCGCCATCTCATACAGATGGCGCTTTTTTATTATAGATATTAATCACTTTACTCAATACCAA
TATACAGCTCGACTTGACCA

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MSDFDKNRNTQSQTQATHQQATLDLSIALLERPSVTPDDDGCQDILSERLTQAGFDCEFMYYGDRQAKGEHAEVKNLWAR
RGNTNPVICFAGHTDVVPTGDEKNWTYPPFTPTIADGYLWARGAADMKTGIAAFTVAAERFVANHPEHNGSIAFLITSDE
EGPSINGTVKVVETLEARNEKITYCLVGEPSSTDTLGDIIKNGRRGSLGAVLTVTGKQGHVAYPHLASNPIHAAMSALAE
LTNATWDNGNDYFPATSLQISNINSGTGATNVIPETLEAVFNFRFSTETSEDELKAKTHAIFDRYFADSKAAYDIHWKLS
GQPFLTPEGKLVSACQQAIKSVTGTDTTLSTSGGTSDGRFIAPTGAQVVELGVRNATIHQVDEKVEVDDLGKLAQIYEGI
LENLLLD

Sequences:

>Translated_407_residues
MSDFDKNRNTQSQTQATHQQATLDLSIALLERPSVTPDDDGCQDILSERLTQAGFDCEFMYYGDRQAKGEHAEVKNLWAR
RGNTNPVICFAGHTDVVPTGDEKNWTYPPFTPTIADGYLWARGAADMKTGIAAFTVAAERFVANHPEHNGSIAFLITSDE
EGPSINGTVKVVETLEARNEKITYCLVGEPSSTDTLGDIIKNGRRGSLGAVLTVTGKQGHVAYPHLASNPIHAAMSALAE
LTNATWDNGNDYFPATSLQISNINSGTGATNVIPETLEAVFNFRFSTETSEDELKAKTHAIFDRYFADSKAAYDIHWKLS
GQPFLTPEGKLVSACQQAIKSVTGTDTTLSTSGGTSDGRFIAPTGAQVVELGVRNATIHQVDEKVEVDDLGKLAQIYEGI
LENLLLD
>Mature_406_residues
SDFDKNRNTQSQTQATHQQATLDLSIALLERPSVTPDDDGCQDILSERLTQAGFDCEFMYYGDRQAKGEHAEVKNLWARR
GNTNPVICFAGHTDVVPTGDEKNWTYPPFTPTIADGYLWARGAADMKTGIAAFTVAAERFVANHPEHNGSIAFLITSDEE
GPSINGTVKVVETLEARNEKITYCLVGEPSSTDTLGDIIKNGRRGSLGAVLTVTGKQGHVAYPHLASNPIHAAMSALAEL
TNATWDNGNDYFPATSLQISNINSGTGATNVIPETLEAVFNFRFSTETSEDELKAKTHAIFDRYFADSKAAYDIHWKLSG
QPFLTPEGKLVSACQQAIKSVTGTDTTLSTSGGTSDGRFIAPTGAQVVELGVRNATIHQVDEKVEVDDLGKLAQIYEGIL
ENLLLD

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=384, Percent_Identity=55.2083333333333, Blast_Score=424, Evalue=1e-120,
Organism=Escherichia coli, GI1790395, Length=286, Percent_Identity=26.2237762237762, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24649202, Length=392, Percent_Identity=22.4489795918367, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24649204, Length=392, Percent_Identity=22.4489795918367, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 44078; Mature: 43947

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFDKNRNTQSQTQATHQQATLDLSIALLERPSVTPDDDGCQDILSERLTQAGFDCEFM
CCCCCCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEE
YYGDRQAKGEHAEVKNLWARRGNTNPVICFAGHTDVVPTGDEKNWTYPPFTPTIADGYLW
EECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
ARGAADMKTGIAAFTVAAERFVANHPEHNGSIAFLITSDEEGPSINGTVKVVETLEARNE
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHEEEHHHCCCC
KITYCLVGEPSSTDTLGDIIKNGRRGSLGAVLTVTGKQGHVAYPHLASNPIHAAMSALAE
EEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHH
LTNATWDNGNDYFPATSLQISNINSGTGATNVIPETLEAVFNFRFSTETSEDELKAKTHA
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCEEECCCCCHHHHHHHHHH
IFDRYFADSKAAYDIHWKLSGQPFLTPEGKLVSACQQAIKSVTGTDTTLSTSGGTSDGRF
HHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEE
IAPTGAQVVELGVRNATIHQVDEKVEVDDLGKLAQIYEGILENLLLD
ECCCCCCEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDFDKNRNTQSQTQATHQQATLDLSIALLERPSVTPDDDGCQDILSERLTQAGFDCEFM
CCCCCCCCCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEE
YYGDRQAKGEHAEVKNLWARRGNTNPVICFAGHTDVVPTGDEKNWTYPPFTPTIADGYLW
EECCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEE
ARGAADMKTGIAAFTVAAERFVANHPEHNGSIAFLITSDEEGPSINGTVKVVETLEARNE
ECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCHHHEEEHHHCCCC
KITYCLVGEPSSTDTLGDIIKNGRRGSLGAVLTVTGKQGHVAYPHLASNPIHAAMSALAE
EEEEEEECCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCCEECCCCCCCHHHHHHHHHHH
LTNATWDNGNDYFPATSLQISNINSGTGATNVIPETLEAVFNFRFSTETSEDELKAKTHA
HHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHCEEECCCCCHHHHHHHHHH
IFDRYFADSKAAYDIHWKLSGQPFLTPEGKLVSACQQAIKSVTGTDTTLSTSGGTSDGRF
HHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCEE
IAPTGAQVVELGVRNATIHQVDEKVEVDDLGKLAQIYEGILENLLLD
ECCCCCCEEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA