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The map label for this gene is gatA [H]

Identifier: 93005306

GI number: 93005306

Start: 584371

End: 585867

Strand: Reverse

Name: gatA [H]

Synonym: Pcryo_0476

Alternate gene names: 93005306

Gene position: 585867-584371 (Counterclockwise)

Preceding gene: 93005307

Following gene: 93005305

Centisome position: 19.15

GC content: 46.56

Gene sequence:

>1497_bases
ATGTCTGAACTTCATTTATTAAGTACCCAGCAGCTCATTACTGGTCTGCAAGACAAACAATTTAGCAGCCTTGAGCTGAC
TGACCACTACATCAAACGTATCAACGCGCTTGATAGCAAGATCAACAGTTTTATCACTCATACCTCTGAGACAGCGCGCG
CGCAAGCAAAAGCGGCGGACGAGATGCGTGTACAAGGGGACAAACGCCCATTACTTGGCGTACCCATGGCGCATAAAGAT
ATTTTTTGTACTCAAGGCGTATTGACGACCTGTGGCTCTAAAATGCTGCATAATTTTATCTCTCCTTATGATGCGACCAT
CGTCACTAACATTGATAAAGCTGGAATGATTAGCCTTGGTAAACTCAATATGGATGAGTTTGCGATGGGCTCAGACAATG
AAAGCTCATATTATGGTGCGGTGCACAATCCTTGGAACATAGCGCATGTACCTGGTGGCTCATCAGGTGGTAGTGCAGCA
GCCGTTGCAGCAGGATTTGTACCCGTTGCGACTGGTAGTGATACCGGTGGCTCTATTCGTCAGCCTGCCTCATTTTGCGG
CTTAACGGGCATCAAACCTACTTATGGTCGCGTCTCTCGCTTCGGTATGATTGCTTATGCCTCGAGTCTTGATCAAGCCG
GTAGTATGGGTCGCAGTGCCATGGACTGTGCCTTCCTATTACAGCCAATGATTGGTCATGATCCACGCGATGCGACTTCT
ATCAAATACGACATGCCTGATTATGTACAAGATTTAAATGACGCTGCTGCCAGCGCTGGTGATAAACCGTTTGCCGGTTT
ACGCATTGGTGTTGCTAAAGAATACTTTAGTAAAGGACTTGATACTGAAGTCGAACAAGCAGCTCGTGCAGCGTTAAAGA
AATATGAAGAACTTGGCGCGACCATTGTAGAGGTGACAATCACCGATCCAGAGATCACCCTTGCTACCTATTATATGCTC
GCCCCAGCTGAAGCATCATCAAACCTATCACGCTTCGATGGTGTGCGCTTTGGGTATCGCTGTGAAAACCCTAAAGACTT
GATTGACTTATATACGCGCTCGCGTTCTGAAGGCTTTGGTCCTGAAGTACAGCGCCGTATTTTGTCAGGGACTTATGCGC
TATCTGCTGGTTACTTTGATGCCTATTACACTAAGGCACAAAAAGTGCGCCGCATAATTATCAAAGACTTCGAAGATGCT
TTTGCCAACTGTGATGTGATCGCCAGTCCAACGGCGCCAACAGCGGCTTATAAACTTGGTGAAGACCTTGATCCTGCAAC
GATGTACCTAGGTGATGTTTATACCATTGGTGTCAACTTAGCGGGTCTACCTGCGCTTAGTCAGCCAGTTGGTTTAACCT
CAGCTGGATTGCCTATTGGTTTGCAATTGATTGGACAATATTGGCAAGAGAGCAAATTGCTCTCTACTGCTCATTTATTC
CAGCAGCATACAGATCATCATCTGCAACACTCTACCATCGCGAAGGAGACGGTATAA

Upstream 100 bases:

>100_bases
ATGGCTTGTATTTGGTACCGCAAGTGATTGAATAGGCGGCTGTCGCTCTCTGATTTCGTATCTTACTTTATGCTATTAAC
ATTTTGACGGACGACACCTT

Downstream 100 bases:

>100_bases
TGAATACAGCAACTACTACTGATAACAACGCCGTTCGCAAACACGATGTTCGTAAAGAGCTATTCGTCGATGGTTATGAA
GTGGTCATCGGTATCGAGAT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MSELHLLSTQQLITGLQDKQFSSLELTDHYIKRINALDSKINSFITHTSETARAQAKAADEMRVQGDKRPLLGVPMAHKD
IFCTQGVLTTCGSKMLHNFISPYDATIVTNIDKAGMISLGKLNMDEFAMGSDNESSYYGAVHNPWNIAHVPGGSSGGSAA
AVAAGFVPVATGSDTGGSIRQPASFCGLTGIKPTYGRVSRFGMIAYASSLDQAGSMGRSAMDCAFLLQPMIGHDPRDATS
IKYDMPDYVQDLNDAAASAGDKPFAGLRIGVAKEYFSKGLDTEVEQAARAALKKYEELGATIVEVTITDPEITLATYYML
APAEASSNLSRFDGVRFGYRCENPKDLIDLYTRSRSEGFGPEVQRRILSGTYALSAGYFDAYYTKAQKVRRIIIKDFEDA
FANCDVIASPTAPTAAYKLGEDLDPATMYLGDVYTIGVNLAGLPALSQPVGLTSAGLPIGLQLIGQYWQESKLLSTAHLF
QQHTDHHLQHSTIAKETV

Sequences:

>Translated_498_residues
MSELHLLSTQQLITGLQDKQFSSLELTDHYIKRINALDSKINSFITHTSETARAQAKAADEMRVQGDKRPLLGVPMAHKD
IFCTQGVLTTCGSKMLHNFISPYDATIVTNIDKAGMISLGKLNMDEFAMGSDNESSYYGAVHNPWNIAHVPGGSSGGSAA
AVAAGFVPVATGSDTGGSIRQPASFCGLTGIKPTYGRVSRFGMIAYASSLDQAGSMGRSAMDCAFLLQPMIGHDPRDATS
IKYDMPDYVQDLNDAAASAGDKPFAGLRIGVAKEYFSKGLDTEVEQAARAALKKYEELGATIVEVTITDPEITLATYYML
APAEASSNLSRFDGVRFGYRCENPKDLIDLYTRSRSEGFGPEVQRRILSGTYALSAGYFDAYYTKAQKVRRIIIKDFEDA
FANCDVIASPTAPTAAYKLGEDLDPATMYLGDVYTIGVNLAGLPALSQPVGLTSAGLPIGLQLIGQYWQESKLLSTAHLF
QQHTDHHLQHSTIAKETV
>Mature_497_residues
SELHLLSTQQLITGLQDKQFSSLELTDHYIKRINALDSKINSFITHTSETARAQAKAADEMRVQGDKRPLLGVPMAHKDI
FCTQGVLTTCGSKMLHNFISPYDATIVTNIDKAGMISLGKLNMDEFAMGSDNESSYYGAVHNPWNIAHVPGGSSGGSAAA
VAAGFVPVATGSDTGGSIRQPASFCGLTGIKPTYGRVSRFGMIAYASSLDQAGSMGRSAMDCAFLLQPMIGHDPRDATSI
KYDMPDYVQDLNDAAASAGDKPFAGLRIGVAKEYFSKGLDTEVEQAARAALKKYEELGATIVEVTITDPEITLATYYMLA
PAEASSNLSRFDGVRFGYRCENPKDLIDLYTRSRSEGFGPEVQRRILSGTYALSAGYFDAYYTKAQKVRRIIIKDFEDAF
ANCDVIASPTAPTAAYKLGEDLDPATMYLGDVYTIGVNLAGLPALSQPVGLTSAGLPIGLQLIGQYWQESKLLSTAHLFQ
QHTDHHLQHSTIAKETV

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=35.4455445544554, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI195972892, Length=505, Percent_Identity=26.3366336633663, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI166795287, Length=133, Percent_Identity=35.3383458646617, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=451, Percent_Identity=37.9157427937916, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17537465, Length=496, Percent_Identity=25.4032258064516, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=315, Percent_Identity=27.6190476190476, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=313, Percent_Identity=26.8370607028754, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17538252, Length=201, Percent_Identity=29.3532338308458, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=199, Percent_Identity=31.1557788944724, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=199, Percent_Identity=31.1557788944724, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=199, Percent_Identity=29.1457286432161, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=467, Percent_Identity=35.5460385438972, Blast_Score=244, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=456, Percent_Identity=28.0701754385965, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6320448, Length=186, Percent_Identity=30.6451612903226, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24648113, Length=512, Percent_Identity=37.890625, Blast_Score=288, Evalue=6e-78,
Organism=Drosophila melanogaster, GI24644968, Length=494, Percent_Identity=27.5303643724696, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24652985, Length=503, Percent_Identity=26.0437375745527, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922090, Length=503, Percent_Identity=26.0437375745527, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24652981, Length=503, Percent_Identity=26.0437375745527, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24652983, Length=503, Percent_Identity=26.0437375745527, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078093, Length=509, Percent_Identity=27.1119842829077, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21356731, Length=500, Percent_Identity=25.2, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45550774, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648435, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648437, Length=491, Percent_Identity=24.4399185336049, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648441, Length=428, Percent_Identity=25.2336448598131, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648439, Length=428, Percent_Identity=25.2336448598131, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53768; Mature: 53637

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELHLLSTQQLITGLQDKQFSSLELTDHYIKRINALDSKINSFITHTSETARAQAKAAD
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EMRVQGDKRPLLGVPMAHKDIFCTQGVLTTCGSKMLHNFISPYDATIVTNIDKAGMISLG
HHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEC
KLNMDEFAMGSDNESSYYGAVHNPWNIAHVPGGSSGGSAAAVAAGFVPVATGSDTGGSIR
CCCHHHHCCCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCCC
QPASFCGLTGIKPTYGRVSRFGMIAYASSLDQAGSMGRSAMDCAFLLQPMIGHDPRDATS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCE
IKYDMPDYVQDLNDAAASAGDKPFAGLRIGVAKEYFSKGLDTEVEQAARAALKKYEELGA
EEECCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
TIVEVTITDPEITLATYYMLAPAEASSNLSRFDGVRFGYRCENPKDLIDLYTRSRSEGFG
EEEEEEECCCCEEEEEEEEEECCHHCCCHHHHCCCCCCEECCCHHHHHHHHHHCCCCCCC
PEVQRRILSGTYALSAGYFDAYYTKAQKVRRIIIKDFEDAFANCDVIASPTAPTAAYKLG
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHCC
EDLDPATMYLGDVYTIGVNLAGLPALSQPVGLTSAGLPIGLQLIGQYWQESKLLSTAHLF
CCCCHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QQHTDHHLQHSTIAKETV
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SELHLLSTQQLITGLQDKQFSSLELTDHYIKRINALDSKINSFITHTSETARAQAKAAD
CCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
EMRVQGDKRPLLGVPMAHKDIFCTQGVLTTCGSKMLHNFISPYDATIVTNIDKAGMISLG
HHHHCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEC
KLNMDEFAMGSDNESSYYGAVHNPWNIAHVPGGSSGGSAAAVAAGFVPVATGSDTGGSIR
CCCHHHHCCCCCCCCCEEECCCCCEEEEECCCCCCCCCHHHHHHCCEEEECCCCCCCCCC
QPASFCGLTGIKPTYGRVSRFGMIAYASSLDQAGSMGRSAMDCAFLLQPMIGHDPRDATS
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCE
IKYDMPDYVQDLNDAAASAGDKPFAGLRIGVAKEYFSKGLDTEVEQAARAALKKYEELGA
EEECCHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
TIVEVTITDPEITLATYYMLAPAEASSNLSRFDGVRFGYRCENPKDLIDLYTRSRSEGFG
EEEEEEECCCCEEEEEEEEEECCHHCCCHHHHCCCCCCEECCCHHHHHHHHHHCCCCCCC
PEVQRRILSGTYALSAGYFDAYYTKAQKVRRIIIKDFEDAFANCDVIASPTAPTAAYKLG
HHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHHHCC
EDLDPATMYLGDVYTIGVNLAGLPALSQPVGLTSAGLPIGLQLIGQYWQESKLLSTAHLF
CCCCHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QQHTDHHLQHSTIAKETV
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA