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The map label for this gene is gatB [H]

Identifier: 93005305

GI number: 93005305

Start: 582842

End: 584371

Strand: Reverse

Name: gatB [H]

Synonym: Pcryo_0475

Alternate gene names: 93005305

Gene position: 584371-582842 (Counterclockwise)

Preceding gene: 93005306

Following gene: 93005304

Centisome position: 19.1

GC content: 47.25

Gene sequence:

>1530_bases
ATGAATACAGCAACTACTACTGATAACAACGCCGTTCGCAAACACGATGTTCGTAAAGAGCTATTCGTCGATGGTTATGA
AGTGGTCATCGGTATCGAGATTCACTGTCAGCTGAACACAGAAAGTAAGATATTCTCGAGCGCGCCGACTGACTTTGGTC
ACGAGCCAAACAGCCAAGCCAGTATTGTAGATTTAGGCTTACCGGGTGTCTTGCCAGTGCTAAATGCTGGTGTGGTTGAT
CGTGCGCTAAAATTCGGTATCGGCGTCAACGCTGAGCTGGGTCTATTTAATACTTTTGACCGCAAAAACTACTTTTACCC
TGATTTACCGAAAGGCTATCAAATCACCCAGATGGCCAACCCTATCGTTGGCGAAGGCTATATAGATGTCGTGGTCAATG
AAGGTGAAAAGAACGAATATCCAAAACGTATGGGTATCACCCGCGCGCATCTAGAAGAAGATGCTGGTAAATCAGTACAC
GATGCCGTCGATGGTATGACGGGCGTGGATCTAAACCGCGCTGGTACGCCTTTGATTGAAATCGTCTCTGAACCTGATAT
GCGCTCAGCTCACGAAGCACTGGCCTATATCAAAGCCATTCATCAGCTGGTCACGTGGCTAGGTATCTCAGATGCTATCA
TGGCAGAAGGCTCATTCCGCTGTGATTGTAACGTCTCTATCCGTAAACCTGGTGCTGAGTTGGGGACGCGTACTGAGCTT
AAGAACTTAAACTCGTTCCGCTTCATCGAGCGTGCCATCAATCGCGAAATCGAGCGTCAGATTGACATTTTAGAAGACGG
CGGTAAAGTTGTACAAGCAACGATGCTTTATGACCCAGAGCGCGACGAAACTCGTGTTATGCGTACCAAAGAAGACGCCA
ATGATTATCGCTACTTTCCTGATCCTGACTTGCTACCTGTGCGTATCGAGCAGCATACGGTTGATAGCATTCGTGCAGCG
ATGCCTGAGCTACCGGTTGCCCGCCGTGCGCGTTTTGAAGAAGCGCTTGGTCTTTCAGAATATGACGCTCGTATTTTGAC
TGGTAGCCGTCAAATTGCTGATTACTTTGAAAATGTGGTCGCAGAAGTTGGGCAAGCAGATGCCAAAATGGCAGCTAACT
GGGTAATGGGCGACCTACTCGGTGCGTTGAATAAAGATGATAAGGAAATTACTGACTCCCCTATCAGCGCCAAGCAATTG
GCTGGTATGCTATCACGCATCAAAGACGATACCCTATCTGGCAAACTGGCTAAGAAGGTCTTTAGTGCCTTATATGAGCG
CGAAGGTGGCGATGCTGATGATGCAGCAGATAAAATCATCGAGGAAAAAGGTCTTAAGCAAGAAACCGATACGGGCGCTA
TCAAAGCCATCGTTGAAGACGTCATTGCTAAAAATGCAGCGATGGTTGAAGAGTATCGCGGCGGTAAAGAAAAAGCCTTC
AATGGTTTAGTTGGTCAAGTAATGAAAGCCAGTCGCGGTAGTGCCAATCCACAGCAAGTCAACCAAATTCTGAAAGAGTT
ACTGGGTTAA

Upstream 100 bases:

>100_bases
AATATTGGCAAGAGAGCAAATTGCTCTCTACTGCTCATTTATTCCAGCAGCATACAGATCATCATCTGCAACACTCTACC
ATCGCGAAGGAGACGGTATA

Downstream 100 bases:

>100_bases
GGTTGACTGCTCATTGGTTATACTGGCTATGATATATAAAATGCCATTGTAGATTTTGGCTGAAATAAATAGCTCTTGCA
ATATACATAATTTTGCGTAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNTATTTDNNAVRKHDVRKELFVDGYEVVIGIEIHCQLNTESKIFSSAPTDFGHEPNSQASIVDLGLPGVLPVLNAGVVD
RALKFGIGVNAELGLFNTFDRKNYFYPDLPKGYQITQMANPIVGEGYIDVVVNEGEKNEYPKRMGITRAHLEEDAGKSVH
DAVDGMTGVDLNRAGTPLIEIVSEPDMRSAHEALAYIKAIHQLVTWLGISDAIMAEGSFRCDCNVSIRKPGAELGTRTEL
KNLNSFRFIERAINREIERQIDILEDGGKVVQATMLYDPERDETRVMRTKEDANDYRYFPDPDLLPVRIEQHTVDSIRAA
MPELPVARRARFEEALGLSEYDARILTGSRQIADYFENVVAEVGQADAKMAANWVMGDLLGALNKDDKEITDSPISAKQL
AGMLSRIKDDTLSGKLAKKVFSALYEREGGDADDAADKIIEEKGLKQETDTGAIKAIVEDVIAKNAAMVEEYRGGKEKAF
NGLVGQVMKASRGSANPQQVNQILKELLG

Sequences:

>Translated_509_residues
MNTATTTDNNAVRKHDVRKELFVDGYEVVIGIEIHCQLNTESKIFSSAPTDFGHEPNSQASIVDLGLPGVLPVLNAGVVD
RALKFGIGVNAELGLFNTFDRKNYFYPDLPKGYQITQMANPIVGEGYIDVVVNEGEKNEYPKRMGITRAHLEEDAGKSVH
DAVDGMTGVDLNRAGTPLIEIVSEPDMRSAHEALAYIKAIHQLVTWLGISDAIMAEGSFRCDCNVSIRKPGAELGTRTEL
KNLNSFRFIERAINREIERQIDILEDGGKVVQATMLYDPERDETRVMRTKEDANDYRYFPDPDLLPVRIEQHTVDSIRAA
MPELPVARRARFEEALGLSEYDARILTGSRQIADYFENVVAEVGQADAKMAANWVMGDLLGALNKDDKEITDSPISAKQL
AGMLSRIKDDTLSGKLAKKVFSALYEREGGDADDAADKIIEEKGLKQETDTGAIKAIVEDVIAKNAAMVEEYRGGKEKAF
NGLVGQVMKASRGSANPQQVNQILKELLG
>Mature_509_residues
MNTATTTDNNAVRKHDVRKELFVDGYEVVIGIEIHCQLNTESKIFSSAPTDFGHEPNSQASIVDLGLPGVLPVLNAGVVD
RALKFGIGVNAELGLFNTFDRKNYFYPDLPKGYQITQMANPIVGEGYIDVVVNEGEKNEYPKRMGITRAHLEEDAGKSVH
DAVDGMTGVDLNRAGTPLIEIVSEPDMRSAHEALAYIKAIHQLVTWLGISDAIMAEGSFRCDCNVSIRKPGAELGTRTEL
KNLNSFRFIERAINREIERQIDILEDGGKVVQATMLYDPERDETRVMRTKEDANDYRYFPDPDLLPVRIEQHTVDSIRAA
MPELPVARRARFEEALGLSEYDARILTGSRQIADYFENVVAEVGQADAKMAANWVMGDLLGALNKDDKEITDSPISAKQL
AGMLSRIKDDTLSGKLAKKVFSALYEREGGDADDAADKIIEEKGLKQETDTGAIKAIVEDVIAKNAAMVEEYRGGKEKAF
NGLVGQVMKASRGSANPQQVNQILKELLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=498, Percent_Identity=37.14859437751, Blast_Score=337, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI115534094, Length=492, Percent_Identity=31.0975609756098, Blast_Score=209, Evalue=2e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=528, Percent_Identity=32.1969696969697, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI45550808, Length=493, Percent_Identity=33.6713995943205, Blast_Score=286, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 56077; Mature: 56077

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTATTTDNNAVRKHDVRKELFVDGYEVVIGIEIHCQLNTESKIFSSAPTDFGHEPNSQA
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEEEEECCCHHHHHCCCCCCCCCCCCCC
SIVDLGLPGVLPVLNAGVVDRALKFGIGVNAELGLFNTFDRKNYFYPDLPKGYQITQMAN
EEEEECCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHCC
PIVGEGYIDVVVNEGEKNEYPKRMGITRAHLEEDAGKSVHDAVDGMTGVDLNRAGTPLIE
CCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHH
IVSEPDMRSAHEALAYIKAIHQLVTWLGISDAIMAEGSFRCDCNVSIRKPGAELGTRTEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCEEEECCCHHHCCHHHH
KNLNSFRFIERAINREIERQIDILEDGGKVVQATMLYDPERDETRVMRTKEDANDYRYFP
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCC
DPDLLPVRIEQHTVDSIRAAMPELPVARRARFEEALGLSEYDARILTGSRQIADYFENVV
CCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHCHHHHCCCHHHHHHHHHHH
AEVGQADAKMAANWVMGDLLGALNKDDKEITDSPISAKQLAGMLSRIKDDTLSGKLAKKV
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FSALYEREGGDADDAADKIIEEKGLKQETDTGAIKAIVEDVIAKNAAMVEEYRGGKEKAF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
NGLVGQVMKASRGSANPQQVNQILKELLG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MNTATTTDNNAVRKHDVRKELFVDGYEVVIGIEIHCQLNTESKIFSSAPTDFGHEPNSQA
CCCCCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEEEEECCCHHHHHCCCCCCCCCCCCCC
SIVDLGLPGVLPVLNAGVVDRALKFGIGVNAELGLFNTFDRKNYFYPDLPKGYQITQMAN
EEEEECCCCHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHCC
PIVGEGYIDVVVNEGEKNEYPKRMGITRAHLEEDAGKSVHDAVDGMTGVDLNRAGTPLIE
CCCCCCEEEEEEECCCCCCCHHHHCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCHHH
IVSEPDMRSAHEALAYIKAIHQLVTWLGISDAIMAEGSFRCDCNVSIRKPGAELGTRTEL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEECCEEEECCCHHHCCHHHH
KNLNSFRFIERAINREIERQIDILEDGGKVVQATMLYDPERDETRVMRTKEDANDYRYFP
HCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCCCCCCCC
DPDLLPVRIEQHTVDSIRAAMPELPVARRARFEEALGLSEYDARILTGSRQIADYFENVV
CCCCEEEEEHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHCHHHHCCCHHHHHHHHHHH
AEVGQADAKMAANWVMGDLLGALNKDDKEITDSPISAKQLAGMLSRIKDDTLSGKLAKKV
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FSALYEREGGDADDAADKIIEEKGLKQETDTGAIKAIVEDVIAKNAAMVEEYRGGKEKAF
HHHHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
NGLVGQVMKASRGSANPQQVNQILKELLG
HHHHHHHHHHCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA