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The map label for this gene is paaF [C]

Identifier: 93005172

GI number: 93005172

Start: 413536

End: 414342

Strand: Direct

Name: paaF [C]

Synonym: Pcryo_0341

Alternate gene names: 93005172

Gene position: 413536-414342 (Clockwise)

Preceding gene: 93005171

Following gene: 93005173

Centisome position: 13.51

GC content: 46.47

Gene sequence:

>807_bases
ATGCAACAAAATTTGAATCATTATAAAAGTCTATTGGTTAACGTTGAGCAGCACGTTGCAACAGTAACCTTGAATCGCCC
TGAGATACGTAACGCCTTTAATGATGAAATGATTGCTGAGTTGACCCATGCTTTCAAGACGCTTGGCGAGGATGAGCAAG
TACGCGTTATCGTTTTAGCAGCGGCCGGTAAAGCGTTCTGTGCGGGTGCCGATTTGAATTGGATGCGCGCCATGGCGGAT
TATAGCTATGAGGAAAACCTAGCGGATGCGGACAAATTGGCACAAATGCTTAAGACTATTTATGAATGCCCAAAACCAAC
GATAGCGGCGGTACAAGGGGATGTTTATGCAGGCGGCATGGGTTTGGTTGCTGTCTGTGATGTAGCTATCGCGGTGAGAA
TTGCCAATTTCTGTCTTAGCGAAGTGCGTCTGGGCTTAGCACCAGCAACCATTAGCCCTTATGTGATTAGAGCTTTAGGT
GCCAGAGCCTCACAGCGTTATTTTCTCAGTGCGGAAGTATTTGATAGCAAAAAAGCCCGTCAGCTTGGCTTTATCCATGA
ACGCGTCAGTGAAGAGTCGCTTGATGATGCAGTTGCAGAATTTTGTGCCAAAGTCGTTAAAAATAGCCCTGAGGCTATGA
AAACCTGCAAGCGCTTATTGCATGAAGTCGCGGGCGCACCGATTACCGATGAACTAATTGCCGATACAGTCAAAGGCATC
GCTGATATTCGCTCATCAGAGCAAGGTAAAGAGGGTGTGCAAGCATTTTTGCAAAAGCGCCAACCTGATTGGCTAGTAGC
AAAATAG

Upstream 100 bases:

>100_bases
CCGATTGAAGAGACAAAATTTGGTGTCTTTAGAATGTAGCAATGAACGGTATATTGACAGTCATTTAGTGCTCATCAATA
CATAAAATATAGAGAAAGAT

Downstream 100 bases:

>100_bases
CCATGTATCAATAAAAAATATTTTTGCTAAATTGCCAATGAATACTAAAACCAGTTTAGCGTAAATAGTCATACGTGAAT
ATCATCCTACCGTTTGATTA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MQQNLNHYKSLLVNVEQHVATVTLNRPEIRNAFNDEMIAELTHAFKTLGEDEQVRVIVLAAAGKAFCAGADLNWMRAMAD
YSYEENLADADKLAQMLKTIYECPKPTIAAVQGDVYAGGMGLVAVCDVAIAVRIANFCLSEVRLGLAPATISPYVIRALG
ARASQRYFLSAEVFDSKKARQLGFIHERVSEESLDDAVAEFCAKVVKNSPEAMKTCKRLLHEVAGAPITDELIADTVKGI
ADIRSSEQGKEGVQAFLQKRQPDWLVAK

Sequences:

>Translated_268_residues
MQQNLNHYKSLLVNVEQHVATVTLNRPEIRNAFNDEMIAELTHAFKTLGEDEQVRVIVLAAAGKAFCAGADLNWMRAMAD
YSYEENLADADKLAQMLKTIYECPKPTIAAVQGDVYAGGMGLVAVCDVAIAVRIANFCLSEVRLGLAPATISPYVIRALG
ARASQRYFLSAEVFDSKKARQLGFIHERVSEESLDDAVAEFCAKVVKNSPEAMKTCKRLLHEVAGAPITDELIADTVKGI
ADIRSSEQGKEGVQAFLQKRQPDWLVAK
>Mature_268_residues
MQQNLNHYKSLLVNVEQHVATVTLNRPEIRNAFNDEMIAELTHAFKTLGEDEQVRVIVLAAAGKAFCAGADLNWMRAMAD
YSYEENLADADKLAQMLKTIYECPKPTIAAVQGDVYAGGMGLVAVCDVAIAVRIANFCLSEVRLGLAPATISPYVIRALG
ARASQRYFLSAEVFDSKKARQLGFIHERVSEESLDDAVAEFCAKVVKNSPEAMKTCKRLLHEVAGAPITDELIADTVKGI
ADIRSSEQGKEGVQAFLQKRQPDWLVAK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=29.4117647058824, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI70995211, Length=154, Percent_Identity=35.7142857142857, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=24.901185770751, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI4502327, Length=260, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI37594471, Length=190, Percent_Identity=25.7894736842105, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI37594469, Length=190, Percent_Identity=25.7894736842105, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=30.9803921568627, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=29.8804780876494, Blast_Score=114, Evalue=6e-27,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=29.6577946768061, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI87082183, Length=251, Percent_Identity=25.4980079681275, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI221142681, Length=266, Percent_Identity=28.1954887218045, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788682, Length=208, Percent_Identity=26.4423076923077, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=28.4046692607004, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17554946, Length=253, Percent_Identity=25.6916996047431, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=219, Percent_Identity=28.7671232876712, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=223, Percent_Identity=25.5605381165919, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17534483, Length=236, Percent_Identity=28.3898305084746, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=227, Percent_Identity=24.6696035242291, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=24.390243902439, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=231, Percent_Identity=24.6753246753247, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560910, Length=198, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24653139, Length=256, Percent_Identity=26.5625, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=25.968992248062, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=25.968992248062, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19920382, Length=248, Percent_Identity=28.2258064516129, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19922422, Length=255, Percent_Identity=23.921568627451, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=26.0416666666667, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24583165, Length=211, Percent_Identity=26.5402843601896, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29388; Mature: 29388

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQNLNHYKSLLVNVEQHVATVTLNRPEIRNAFNDEMIAELTHAFKTLGEDEQVRVIVLA
CCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
AAGKAFCAGADLNWMRAMADYSYEENLADADKLAQMLKTIYECPKPTIAAVQGDVYAGGM
ECCCHHCCCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCEECCH
GLVAVCDVAIAVRIANFCLSEVRLGLAPATISPYVIRALGARASQRYFLSAEVFDSKKAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHEEHHHHHCCHHHH
QLGFIHERVSEESLDDAVAEFCAKVVKNSPEAMKTCKRLLHEVAGAPITDELIADTVKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ADIRSSEQGKEGVQAFLQKRQPDWLVAK
HHHHCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQQNLNHYKSLLVNVEQHVATVTLNRPEIRNAFNDEMIAELTHAFKTLGEDEQVRVIVLA
CCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
AAGKAFCAGADLNWMRAMADYSYEENLADADKLAQMLKTIYECPKPTIAAVQGDVYAGGM
ECCCHHCCCCCHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHCCCCCHHHCCCCCEECCH
GLVAVCDVAIAVRIANFCLSEVRLGLAPATISPYVIRALGARASQRYFLSAEVFDSKKAR
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHEEHHHHHCCHHHH
QLGFIHERVSEESLDDAVAEFCAKVVKNSPEAMKTCKRLLHEVAGAPITDELIADTVKGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
ADIRSSEQGKEGVQAFLQKRQPDWLVAK
HHHHCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA