Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 93005149

GI number: 93005149

Start: 385995

End: 387251

Strand: Direct

Name: glyA [H]

Synonym: Pcryo_0318

Alternate gene names: 93005149

Gene position: 385995-387251 (Clockwise)

Preceding gene: 93005148

Following gene: 93005154

Centisome position: 12.61

GC content: 45.43

Gene sequence:

>1257_bases
ATGTTTAAAGATATCTCTATCAAAGATTTTGATCCAGTACTTGCTGAAGCTATGGCTGCTGAAAGCGTTCGTCAAGAGAA
CCATATTGAGCTTATCGCTTCAGAAAACTACTGCTCACAAGCAGTGATGGAAGCGCAGGGTACTGATTTGACCAACAAAT
ACGCTGAAGGCTATCCTGGTAAGCGCTATTACGGTGGTTGTGAGCATGTTGACGTCGTTGAGCAACTAGCTATTGACCGT
GCAAAAGAATTGTTTGGTGCAGAATATGTGAACGTACAGCCACACTCTGGTAGCCAAGCAAACTCTGCTGTCTTTTTAGC
ATTGCTTGAAGCCAATGATACTGTGCTTGGCATGAGTTTAGATGCTGGTGGTCACTTAACGCATGGCGCACATATCAACT
TCTCAGGTCTAAACTACAATGCAGTACAGTATGGTTTGGTCGAAGGCACTGGTCTTATCGATTATGACCAAGTTGAGAGC
TTGGCAAAAGAGCATAAGCCTAAAATGATCATCGCTGGTTTTTCAGCGTATTCACAAGTAGTTGATTGGGCGCGTTTCCG
TGAAATCGCTGACGAAGTAGGCGCTTACTTACTAGTCGATATGGCTCACGTTGCTGGTCTTATCGCTGGCGGCGTTTACC
CAAGCCCAGTACCATTTGCTGATGTAGTGACCACGACTACGCACAAAACCTTACGTGGCCCACGTTCAGGTATGATTCTT
GCTCGTGATGAAGTACTTGCTAAAAAGCTCAATTCTGCCGTATTCCCAGGTAACCAAGGTGGCCCGTTGATGCATGTCAT
CGCGGCAAAAGCAGTTTGCTTTAAAGAAGCATTAGAAGAGAACTTCAAAACCTATCAGCAGCAAGTGGTTAAAAACGCTC
AAGCCATGGCGAAAGTCATTCAAGATCGCGGCTACGAAATTATCTCTGGTGGCACTGAAAACCATCTGATGCTTATCAGC
TTAGTTAAGCAAGAGATGACCGGTAAAGAAGCGGATAAATGGTTGGGTGACGCACACATTACGGTCAATAAAAACGCCGT
ACCAAACGATCCAAAATCTCCGTTTGTGACTTCTGGTATCCGTATTGGTACACCAGCGATTACCACTCGCGGCTTCAACG
AAGCACAAGCAGGTGATTTGGCAGGTTGGATTTGTGATGTTCTTGACAGCCGTGGCGATGAAGCAGTGACTGCTGAAGTA
CGTGGCAAAGTAGAAGCGATCTGTAAAGAGCTTCCTGTCTATGCTAAAAACCAGTAA

Upstream 100 bases:

>100_bases
TAAGCTCATGCCTTTTGCTTGCCTAGTTCAAACTTCTTTTGCTACTCGCTTCTTTCAACGACCGTTATTACTAATAAACG
TCACCACTTAGGACTTTACT

Downstream 100 bases:

>100_bases
TTTCTAATTAATTGGTTTTATAAGTCAGAAATAAAAAAACCGCTTAGCTTTTGCTAGGCGGTTTTTTTGTGAGTATATGA
ATCAGCTTTTTATTTCATCA

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MFKDISIKDFDPVLAEAMAAESVRQENHIELIASENYCSQAVMEAQGTDLTNKYAEGYPGKRYYGGCEHVDVVEQLAIDR
AKELFGAEYVNVQPHSGSQANSAVFLALLEANDTVLGMSLDAGGHLTHGAHINFSGLNYNAVQYGLVEGTGLIDYDQVES
LAKEHKPKMIIAGFSAYSQVVDWARFREIADEVGAYLLVDMAHVAGLIAGGVYPSPVPFADVVTTTTHKTLRGPRSGMIL
ARDEVLAKKLNSAVFPGNQGGPLMHVIAAKAVCFKEALEENFKTYQQQVVKNAQAMAKVIQDRGYEIISGGTENHLMLIS
LVKQEMTGKEADKWLGDAHITVNKNAVPNDPKSPFVTSGIRIGTPAITTRGFNEAQAGDLAGWICDVLDSRGDEAVTAEV
RGKVEAICKELPVYAKNQ

Sequences:

>Translated_418_residues
MFKDISIKDFDPVLAEAMAAESVRQENHIELIASENYCSQAVMEAQGTDLTNKYAEGYPGKRYYGGCEHVDVVEQLAIDR
AKELFGAEYVNVQPHSGSQANSAVFLALLEANDTVLGMSLDAGGHLTHGAHINFSGLNYNAVQYGLVEGTGLIDYDQVES
LAKEHKPKMIIAGFSAYSQVVDWARFREIADEVGAYLLVDMAHVAGLIAGGVYPSPVPFADVVTTTTHKTLRGPRSGMIL
ARDEVLAKKLNSAVFPGNQGGPLMHVIAAKAVCFKEALEENFKTYQQQVVKNAQAMAKVIQDRGYEIISGGTENHLMLIS
LVKQEMTGKEADKWLGDAHITVNKNAVPNDPKSPFVTSGIRIGTPAITTRGFNEAQAGDLAGWICDVLDSRGDEAVTAEV
RGKVEAICKELPVYAKNQ
>Mature_418_residues
MFKDISIKDFDPVLAEAMAAESVRQENHIELIASENYCSQAVMEAQGTDLTNKYAEGYPGKRYYGGCEHVDVVEQLAIDR
AKELFGAEYVNVQPHSGSQANSAVFLALLEANDTVLGMSLDAGGHLTHGAHINFSGLNYNAVQYGLVEGTGLIDYDQVES
LAKEHKPKMIIAGFSAYSQVVDWARFREIADEVGAYLLVDMAHVAGLIAGGVYPSPVPFADVVTTTTHKTLRGPRSGMIL
ARDEVLAKKLNSAVFPGNQGGPLMHVIAAKAVCFKEALEENFKTYQQQVVKNAQAMAKVIQDRGYEIISGGTENHLMLIS
LVKQEMTGKEADKWLGDAHITVNKNAVPNDPKSPFVTSGIRIGTPAITTRGFNEAQAGDLAGWICDVLDSRGDEAVTAEV
RGKVEAICKELPVYAKNQ

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI261862352, Length=392, Percent_Identity=48.7244897959184, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862350, Length=392, Percent_Identity=48.7244897959184, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862348, Length=392, Percent_Identity=48.7244897959184, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI19923315, Length=392, Percent_Identity=48.7244897959184, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI261862346, Length=392, Percent_Identity=46.9387755102041, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=46.3840399002494, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI22547189, Length=387, Percent_Identity=44.1860465116279, Blast_Score=292, Evalue=4e-79,
Organism=Escherichia coli, GI1788902, Length=415, Percent_Identity=71.3253012048193, Blast_Score=587, Evalue=1e-169,
Organism=Caenorhabditis elegans, GI25144732, Length=390, Percent_Identity=46.6666666666667, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=390, Percent_Identity=46.6666666666667, Blast_Score=346, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=401, Percent_Identity=44.8877805486284, Blast_Score=351, Evalue=1e-97,
Organism=Saccharomyces cerevisiae, GI6323087, Length=399, Percent_Identity=44.3609022556391, Blast_Score=332, Evalue=5e-92,
Organism=Drosophila melanogaster, GI24640005, Length=396, Percent_Identity=47.4747474747475, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=396, Percent_Identity=47.4747474747475, Blast_Score=364, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45235; Mature: 45235

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKDISIKDFDPVLAEAMAAESVRQENHIELIASENYCSQAVMEAQGTDLTNKYAEGYPG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHCCCCC
KRYYGGCEHVDVVEQLAIDRAKELFGAEYVNVQPHSGSQANSAVFLALLEANDTVLGMSL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCEEEEEEE
DAGGHLTHGAHINFSGLNYNAVQYGLVEGTGLIDYDQVESLAKEHKPKMIIAGFSAYSQV
CCCCCEECCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARFREIADEVGAYLLVDMAHVAGLIAGGVYPSPVPFADVVTTTTHKTLRGPRSGMIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
ARDEVLAKKLNSAVFPGNQGGPLMHVIAAKAVCFKEALEENFKTYQQQVVKNAQAMAKVI
EHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDRGYEIISGGTENHLMLISLVKQEMTGKEADKWLGDAHITVNKNAVPNDPKSPFVTSGI
HHCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCCCEECCE
RIGTPAITTRGFNEAQAGDLAGWICDVLDSRGDEAVTAEVRGKVEAICKELPVYAKNQ
EECCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFKDISIKDFDPVLAEAMAAESVRQENHIELIASENYCSQAVMEAQGTDLTNKYAEGYPG
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHCCCCC
KRYYGGCEHVDVVEQLAIDRAKELFGAEYVNVQPHSGSQANSAVFLALLEANDTVLGMSL
CCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEEECCCEEEEEEE
DAGGHLTHGAHINFSGLNYNAVQYGLVEGTGLIDYDQVESLAKEHKPKMIIAGFSAYSQV
CCCCCEECCCEEEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARFREIADEVGAYLLVDMAHVAGLIAGGVYPSPVPFADVVTTTTHKTLRGPRSGMIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
ARDEVLAKKLNSAVFPGNQGGPLMHVIAAKAVCFKEALEENFKTYQQQVVKNAQAMAKVI
EHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QDRGYEIISGGTENHLMLISLVKQEMTGKEADKWLGDAHITVNKNAVPNDPKSPFVTSGI
HHCCCEEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCCCCCCCCCEECCE
RIGTPAITTRGFNEAQAGDLAGWICDVLDSRGDEAVTAEVRGKVEAICKELPVYAKNQ
EECCCCEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA