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The map label for this gene is yddQ [H]

Identifier: 93004883

GI number: 93004883

Start: 61376

End: 61981

Strand: Reverse

Name: yddQ [H]

Synonym: Pcryo_0052

Alternate gene names: 93004883

Gene position: 61981-61376 (Counterclockwise)

Preceding gene: 93004886

Following gene: 93004882

Centisome position: 2.03

GC content: 41.58

Gene sequence:

>606_bases
ATGTCTATTCCCTCTACTTTACTTGGATTAGCTGGTGGTCAATTTAATGAGATTGATTGGTCAAATTGCGCTATCGTCAT
GATTGATTTTCAGAATGAATATGTTGACGGCGCGATGCCGTTAGGTGATGCCGGTAATAAAGCTACTGCGAACGCGTGCC
TGCTACTCGATAGAGCTAGAAGCGAAGCTATCCCGATATTTCATGTTACTCATCACGGGCAGGATAATGATAAAATTTTC
AATCCATTATCATCAAAAGTAGATATTATTAATAGCCTGCAGCCCATTAATGACGAAAAAACCATTGTTAAAATGCACCC
TAATGCATTTTATGATACCGAGCTGCAAGCGCTAATAAAATCGGCTGGTAAGCAACAAATTATTTTTGCAGGCTTTATGA
GCCATATGTGCGTCAGCTCAAGCGTCAGAGCAGCCTTTGATTTGGGTTTTGAAAATTTCGTGTGTCATGATGCTTGTGCC
ACGCGCGATCTGCCTAGTGTTGCAGGACAAGTCATAATGGCTAGTACTGTGCATGATAGTGCGATGGCTGCACTTCAAGA
TAGATTTGCCGCTCTAGTAGCGACTGATAAGTTGACTAACAACTAA

Upstream 100 bases:

>100_bases
TATCAGCATTAGTTGTTAGTATTTAACGTTAGTTAACATTCAAAATTATTTAAGATTTAGCATCTACACTTATATCGTTT
ACTAATTAACGGAGCATAAA

Downstream 100 bases:

>100_bases
TGACTGACGCTAAAATAATGCAAGAAATAGCTCCAGAAACATTACCTAACCCTTGCTAACTGCGTACTGTATGCCGATAT
GCTACACTACTATGCTTGCT

Product: isochorismatase hydrolase

Products: 2,3-Dihydro-2,3-dihydroxybenzoate; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 200

Protein sequence:

>201_residues
MSIPSTLLGLAGGQFNEIDWSNCAIVMIDFQNEYVDGAMPLGDAGNKATANACLLLDRARSEAIPIFHVTHHGQDNDKIF
NPLSSKVDIINSLQPINDEKTIVKMHPNAFYDTELQALIKSAGKQQIIFAGFMSHMCVSSSVRAAFDLGFENFVCHDACA
TRDLPSVAGQVIMASTVHDSAMAALQDRFAALVATDKLTNN

Sequences:

>Translated_201_residues
MSIPSTLLGLAGGQFNEIDWSNCAIVMIDFQNEYVDGAMPLGDAGNKATANACLLLDRARSEAIPIFHVTHHGQDNDKIF
NPLSSKVDIINSLQPINDEKTIVKMHPNAFYDTELQALIKSAGKQQIIFAGFMSHMCVSSSVRAAFDLGFENFVCHDACA
TRDLPSVAGQVIMASTVHDSAMAALQDRFAALVATDKLTNN
>Mature_200_residues
SIPSTLLGLAGGQFNEIDWSNCAIVMIDFQNEYVDGAMPLGDAGNKATANACLLLDRARSEAIPIFHVTHHGQDNDKIFN
PLSSKVDIINSLQPINDEKTIVKMHPNAFYDTELQALIKSAGKQQIIFAGFMSHMCVSSSVRAAFDLGFENFVCHDACAT
RDLPSVAGQVIMASTVHDSAMAALQDRFAALVATDKLTNN

Specific function: Required For Production Of 2,3-Dhb. Also Serves As An Aryl Carrier Protein And Plays A Role In Enterobactin Assembly. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.3.2.1 [C]

Molecular weight: Translated: 21732; Mature: 21601

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPSTLLGLAGGQFNEIDWSNCAIVMIDFQNEYVDGAMPLGDAGNKATANACLLLDRAR
CCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHEEEEECCC
SEAIPIFHVTHHGQDNDKIFNPLSSKVDIINSLQPINDEKTIVKMHPNAFYDTELQALIK
CCCCCEEEEEECCCCCCHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHH
SAGKQQIIFAGFMSHMCVSSSVRAAFDLGFENFVCHDACATRDLPSVAGQVIMASTVHDS
HCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCHHHHHHEEEEHHHHHH
AMAALQDRFAALVATDKLTNN
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SIPSTLLGLAGGQFNEIDWSNCAIVMIDFQNEYVDGAMPLGDAGNKATANACLLLDRAR
CCCHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHEEEEECCC
SEAIPIFHVTHHGQDNDKIFNPLSSKVDIINSLQPINDEKTIVKMHPNAFYDTELQALIK
CCCCCEEEEEECCCCCCHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHH
SAGKQQIIFAGFMSHMCVSSSVRAAFDLGFENFVCHDACATRDLPSVAGQVIMASTVHDS
HCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCHHEEHHHHHCCCCHHHHHHEEEEHHHHHH
AMAALQDRFAALVATDKLTNN
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; Isochorismate [C]

Specific reaction: H2O + Isochorismate --> 2,3-Dihydro-2,3-dihydroxybenzoate + Pyruvate [C]

General reaction: Ether hydrolysis [C]

Inhibitor: Fe2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]