Click here to switch to the map view.

The map label for this gene is gshA [H]

Identifier: 93004874

GI number: 93004874

Start: 50189

End: 51772

Strand: Reverse

Name: gshA [H]

Synonym: Pcryo_0043

Alternate gene names: 93004874

Gene position: 51772-50189 (Counterclockwise)

Preceding gene: 93004875

Following gene: 93004873

Centisome position: 1.69

GC content: 44.32

Gene sequence:

>1584_bases
ATGATCCTTATGAGTAATTTTGCGAGTAGCTTTGTCGATTTTGATATTCCAAACTGGTTTGCCAGCGAGCATTTAGCAGG
CATGTTACGTGGTATCGAAAAAGAAGGATTGCGCGTGACGCCTGATGGTTATTTGGCCCAAACGCCGCACCCTGCCAAAC
TCGGCTCCAAGCTGACTCATCCTTTTATTACGACGGATTACTCTGAAAGCTTACTTGAGCTGATTACCGACCCCAAGTCA
TCACCAAAAGAGACGCTAAATATGCTGCGTCAGCTGCATCTATTGGTCTATAAAGGTATGCCTGAAGATGAGCTGATGTG
GCCGCTGTCTATGCCTTGTATGCTGTCATCAAATGATGAAGATATTCCGCTGGCGGACTATGGTAGCTCTAACACGGGTA
AATTGAAAACTTTGTACCGTAGTGGACTTGGTGTTCGTTATGGTCGCCGGATGCAGACCATTGCAGGTTTACATTATAAT
TTATCATTTGGTGACGATTTATTCACTGCGTGGCAAACACAAACGCCTACTGCACAATCTCTGACATTAACAGAGTTTAA
AAACGATAAATATCTGGGTCTAATTCGTAACTTTAAGCGCCTGACCAGCTTGGTGCTGTATTTATTGGGCGCAAGTCCCA
GTGTCTGCCCTTGTTTTGTTTCAGGGATTGAGCACGACCTTGAGATGTTCAATAATTCAACCTATTATAAACCAACTGCT
ACCAGCCTGCGTATGGGCAAACTTGGTTACACCAATAGTGTGCAAGAGCACCTTGATATCCGCTATAACAATTTGCCCGA
ATATGTCAAAGGTCTGCGCCGTGCGATTCAAACCCCGCACGAAAGCTTTGAGAAGCTTGGCTTAGATGATGAGAATGGCA
ATCCTATTCAAATCAACAATCATATTTTACAAATAGAAAATGAATACTACAGCCCTATTCGTCCTAAGCAAATAGCCATG
AGTGGCGAAAGCCCAACTGAAGCACTTGAGCGTCGTGGTATCGCTTACGTTGAGTTTCGTGCGATTGATTTGGACCCTTA
TAGCGATATCGGTATCCGCTTATCTAGTGCTTGCTTTTTAGAAGTGATGGCGCTTTATTGCTTGCTTAGTGATTCCCCTG
AATTAATGCCTGAGGAAGAAGATGCGCTCGCTATCAATCTTGAGCGCGTTGTTAATGAAGGTCGTTGTGAAAATCTACAA
ATCCTTAATAATGGTGCCGAGCAATCATTAGAGAGCTGGATGCTAATGCATTTGAACCGTATGCAGCCGTTAGCTGCACT
ATTAGATGCCCATTATGGTGGCAATGATTATCGGGCGGCTGTTGCCTTGATGCAAGGCAAAGCTGGTCACTCTGAGTCAA
CTATTTCAGCGCAAGTGAACTCTGATAGCAAGCGCTTGGGCAGTTTATGGCAGTTGGGCTTTACTTTAGCGCAGCAGCAT
CGCGACTCGTTATTGCAGCAGACCCTCAGTCCAAATACCCAAGCAAAATATGAAGTGCTCGCCGAAAAATCCATCTTACA
ACAAGCGGAGATAGAAGCAGCTGAAACAGAAGATTTCATGGATTTCTTGCAGCAATATCGTTAA

Upstream 100 bases:

>100_bases
GTGGTTTTTAGCCTGTCTTTGGGCTAAACTGAAGTCACTATTCTCTATTTTCCGATTAGCCATTGATGGTTTTTTAATCA
ATAATAACCAATAACAACAG

Downstream 100 bases:

>100_bases
TCTTTCATATCTTTCTGTCCTTTATTAACAAGCACTTTATAAGAGTATCTGCCCCATGCTACAGCTGACCACTTACCGTA
ATCTGCAAGTGTTTTTAGTC

Product: glutamate--cysteine ligase, monofunctional

Products: NA

Alternate protein names: Gamma-ECS; GCS; Gamma-glutamylcysteine synthetase [H]

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MILMSNFASSFVDFDIPNWFASEHLAGMLRGIEKEGLRVTPDGYLAQTPHPAKLGSKLTHPFITTDYSESLLELITDPKS
SPKETLNMLRQLHLLVYKGMPEDELMWPLSMPCMLSSNDEDIPLADYGSSNTGKLKTLYRSGLGVRYGRRMQTIAGLHYN
LSFGDDLFTAWQTQTPTAQSLTLTEFKNDKYLGLIRNFKRLTSLVLYLLGASPSVCPCFVSGIEHDLEMFNNSTYYKPTA
TSLRMGKLGYTNSVQEHLDIRYNNLPEYVKGLRRAIQTPHESFEKLGLDDENGNPIQINNHILQIENEYYSPIRPKQIAM
SGESPTEALERRGIAYVEFRAIDLDPYSDIGIRLSSACFLEVMALYCLLSDSPELMPEEEDALAINLERVVNEGRCENLQ
ILNNGAEQSLESWMLMHLNRMQPLAALLDAHYGGNDYRAAVALMQGKAGHSESTISAQVNSDSKRLGSLWQLGFTLAQQH
RDSLLQQTLSPNTQAKYEVLAEKSILQQAEIEAAETEDFMDFLQQYR

Sequences:

>Translated_527_residues
MILMSNFASSFVDFDIPNWFASEHLAGMLRGIEKEGLRVTPDGYLAQTPHPAKLGSKLTHPFITTDYSESLLELITDPKS
SPKETLNMLRQLHLLVYKGMPEDELMWPLSMPCMLSSNDEDIPLADYGSSNTGKLKTLYRSGLGVRYGRRMQTIAGLHYN
LSFGDDLFTAWQTQTPTAQSLTLTEFKNDKYLGLIRNFKRLTSLVLYLLGASPSVCPCFVSGIEHDLEMFNNSTYYKPTA
TSLRMGKLGYTNSVQEHLDIRYNNLPEYVKGLRRAIQTPHESFEKLGLDDENGNPIQINNHILQIENEYYSPIRPKQIAM
SGESPTEALERRGIAYVEFRAIDLDPYSDIGIRLSSACFLEVMALYCLLSDSPELMPEEEDALAINLERVVNEGRCENLQ
ILNNGAEQSLESWMLMHLNRMQPLAALLDAHYGGNDYRAAVALMQGKAGHSESTISAQVNSDSKRLGSLWQLGFTLAQQH
RDSLLQQTLSPNTQAKYEVLAEKSILQQAEIEAAETEDFMDFLQQYR
>Mature_527_residues
MILMSNFASSFVDFDIPNWFASEHLAGMLRGIEKEGLRVTPDGYLAQTPHPAKLGSKLTHPFITTDYSESLLELITDPKS
SPKETLNMLRQLHLLVYKGMPEDELMWPLSMPCMLSSNDEDIPLADYGSSNTGKLKTLYRSGLGVRYGRRMQTIAGLHYN
LSFGDDLFTAWQTQTPTAQSLTLTEFKNDKYLGLIRNFKRLTSLVLYLLGASPSVCPCFVSGIEHDLEMFNNSTYYKPTA
TSLRMGKLGYTNSVQEHLDIRYNNLPEYVKGLRRAIQTPHESFEKLGLDDENGNPIQINNHILQIENEYYSPIRPKQIAM
SGESPTEALERRGIAYVEFRAIDLDPYSDIGIRLSSACFLEVMALYCLLSDSPELMPEEEDALAINLERVVNEGRCENLQ
ILNNGAEQSLESWMLMHLNRMQPLAALLDAHYGGNDYRAAVALMQGKAGHSESTISAQVNSDSKRLGSLWQLGFTLAQQH
RDSLLQQTLSPNTQAKYEVLAEKSILQQAEIEAAETEDFMDFLQQYR

Specific function: Glutathione biosynthesis; first step. [C]

COG id: COG2918

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789044, Length=511, Percent_Identity=39.9217221135029, Blast_Score=352, Evalue=3e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007370
- InterPro:   IPR006334 [H]

Pfam domain/function: PF04262 Glu_cys_ligase [H]

EC number: =6.3.2.2 [H]

Molecular weight: Translated: 59428; Mature: 59428

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMSNFASSFVDFDIPNWFASEHLAGMLRGIEKEGLRVTPDGYLAQTPHPAKLGSKLTH
CEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCCCCC
PFITTDYSESLLELITDPKSSPKETLNMLRQLHLLVYKGMPEDELMWPLSMPCMLSSNDE
CEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCC
DIPLADYGSSNTGKLKTLYRSGLGVRYGRRMQTIAGLHYNLSFGDDLFTAWQTQTPTAQS
CCCCCCCCCCCCCHHHHHHHCCCCCHHCHHHHHHHCEEEEECCCCHHHHHCCCCCCCCCC
LTLTEFKNDKYLGLIRNFKRLTSLVLYLLGASPSVCPCFVSGIEHDLEMFNNSTYYKPTA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
TSLRMGKLGYTNSVQEHLDIRYNNLPEYVKGLRRAIQTPHESFEKLGLDDENGNPIQINN
CCEEECCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCEEEECC
HILQIENEYYSPIRPKQIAMSGESPTEALERRGIAYVEFRAIDLDPYSDIGIRLSSACFL
EEEEECCCCCCCCCCHHEEECCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHH
EVMALYCLLSDSPELMPEEEDALAINLERVVNEGRCENLQILNNGAEQSLESWMLMHLNR
HHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
MQPLAALLDAHYGGNDYRAAVALMQGKAGHSESTISAQVNSDSKRLGSLWQLGFTLAQQH
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
RDSLLQQTLSPNTQAKYEVLAEKSILQQAEIEAAETEDFMDFLQQYR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MILMSNFASSFVDFDIPNWFASEHLAGMLRGIEKEGLRVTPDGYLAQTPHPAKLGSKLTH
CEECCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCHHHCCCCCC
PFITTDYSESLLELITDPKSSPKETLNMLRQLHLLVYKGMPEDELMWPLSMPCMLSSNDE
CEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCEEECCCCC
DIPLADYGSSNTGKLKTLYRSGLGVRYGRRMQTIAGLHYNLSFGDDLFTAWQTQTPTAQS
CCCCCCCCCCCCCHHHHHHHCCCCCHHCHHHHHHHCEEEEECCCCHHHHHCCCCCCCCCC
LTLTEFKNDKYLGLIRNFKRLTSLVLYLLGASPSVCPCFVSGIEHDLEMFNNSTYYKPTA
EEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEECCCC
TSLRMGKLGYTNSVQEHLDIRYNNLPEYVKGLRRAIQTPHESFEKLGLDDENGNPIQINN
CCEEECCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCCCCCCCEEEECC
HILQIENEYYSPIRPKQIAMSGESPTEALERRGIAYVEFRAIDLDPYSDIGIRLSSACFL
EEEEECCCCCCCCCCHHEEECCCCHHHHHHHCCCEEEEEEEEECCCCCCCCCCHHHHHHH
EVMALYCLLSDSPELMPEEEDALAINLERVVNEGRCENLQILNNGAEQSLESWMLMHLNR
HHHHHHHHHCCCCCCCCCCCCCEEEEHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHH
MQPLAALLDAHYGGNDYRAAVALMQGKAGHSESTISAQVNSDSKRLGSLWQLGFTLAQQH
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHH
RDSLLQQTLSPNTQAKYEVLAEKSILQQAEIEAAETEDFMDFLQQYR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA