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The map label for this gene is thiE [H]

Identifier: 92115418

GI number: 92115418

Start: 3679399

End: 3680061

Strand: Direct

Name: thiE [H]

Synonym: Csal_3303

Alternate gene names: 92115418

Gene position: 3679399-3680061 (Clockwise)

Preceding gene: 92115417

Following gene: 92115419

Centisome position: 99.53

GC content: 69.23

Gene sequence:

>663_bases
ATGAACCGGACGCCGGATTGGACACGCGGCATTTACGCCATCACCGATGCGACGCTGCTGCCCGATGACGCTCATCTGTT
TGCGGCATGCGAGCGAGCCCTCGAGGCGGGACTGGCGCTTTTGCAGTATCGCGACAAGTCGGCGGACGCGGGCAAGCGCT
GGCGTCAGGCCTCGGGGCTGGCCGCGCGTTGCCATGCGGCCGGCGTGCCGTTGATCGTCAATGACGATATCGCCCTGGCG
GCGCGTCTGCGGTCACGCGTCGGGTCGCATGTCGGTTTGCATCTCGGCCAGCGGGATGGCTCGCTGCGTGATGCACGTCA
AGCCCTGGGCGACGAGGCGATCATCGGCGCCACCTGTCATGCGCGCCTGGATCTGGCCGAGCGCGCGGCGGCCGAAGGCG
CCAGTTACCTGGCTTTCGGTCGCTTCTTCGCCTCGCGCACCAAGCCGGAGGCACCGCCGGCCTCCCTCGACCTGCTCGGC
GAGGCGGCACGTTTCGGCCTGCCGCGTGTGGCGATCGGGGGGCTCGACGTCCACACTATGCGTCTAGCCCGTGAGGCCGG
GGCGGACCTGCTGGCCACCGTGCATGCCGTCTTCGGTGCCGGTGATCCCGCCGCTGCCGTCAGGGCATTGCAGGCGAGTC
TCGGCGACACGGCACAGCACTGA

Upstream 100 bases:

>100_bases
GCGTGGCCTGGGACAGCCTGGTGCACGCCCAGCGACCGGGACGCGGCCAGGCCTTGCCCGATCGCCTGTGGCGGCTGCCG
CCTTTCGAAAGCGAGGCGAC

Downstream 100 bases:

>100_bases
TTTATCCACAGAGTCGAGCGGCGAGTTGTTCGACCTGTCCAGAGAGTTATCCACAGTTTCTTTTGCGACACGTTATCTTA
GCCACACACAGCCAGAGGAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MNRTPDWTRGIYAITDATLLPDDAHLFAACERALEAGLALLQYRDKSADAGKRWRQASGLAARCHAAGVPLIVNDDIALA
ARLRSRVGSHVGLHLGQRDGSLRDARQALGDEAIIGATCHARLDLAERAAAEGASYLAFGRFFASRTKPEAPPASLDLLG
EAARFGLPRVAIGGLDVHTMRLAREAGADLLATVHAVFGAGDPAAAVRALQASLGDTAQH

Sequences:

>Translated_220_residues
MNRTPDWTRGIYAITDATLLPDDAHLFAACERALEAGLALLQYRDKSADAGKRWRQASGLAARCHAAGVPLIVNDDIALA
ARLRSRVGSHVGLHLGQRDGSLRDARQALGDEAIIGATCHARLDLAERAAAEGASYLAFGRFFASRTKPEAPPASLDLLG
EAARFGLPRVAIGGLDVHTMRLAREAGADLLATVHAVFGAGDPAAAVRALQASLGDTAQH
>Mature_220_residues
MNRTPDWTRGIYAITDATLLPDDAHLFAACERALEAGLALLQYRDKSADAGKRWRQASGLAARCHAAGVPLIVNDDIALA
ARLRSRVGSHVGLHLGQRDGSLRDARQALGDEAIIGATCHARLDLAERAAAEGASYLAFGRFFASRTKPEAPPASLDLLG
EAARFGLPRVAIGGLDVHTMRLAREAGADLLATVHAVFGAGDPAAAVRALQASLGDTAQH

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=176, Percent_Identity=28.4090909090909, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 23101; Mature: 23101

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRTPDWTRGIYAITDATLLPDDAHLFAACERALEAGLALLQYRDKSADAGKRWRQASGL
CCCCCCHHCCEEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
AARCHAAGVPLIVNDDIALAARLRSRVGSHVGLHLGQRDGSLRDARQALGDEAIIGATCH
HHHHHHCCCCEEECCCHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHCCCCCEECCHHH
ARLDLAERAAAEGASYLAFGRFFASRTKPEAPPASLDLLGEAARFGLPRVAIGGLDVHTM
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHH
RLAREAGADLLATVHAVFGAGDPAAAVRALQASLGDTAQH
HHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNRTPDWTRGIYAITDATLLPDDAHLFAACERALEAGLALLQYRDKSADAGKRWRQASGL
CCCCCCHHCCEEEEECCEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
AARCHAAGVPLIVNDDIALAARLRSRVGSHVGLHLGQRDGSLRDARQALGDEAIIGATCH
HHHHHHCCCCEEECCCHHHHHHHHHHHCCHHEEEECCCCCCHHHHHHHCCCCCEECCHHH
ARLDLAERAAAEGASYLAFGRFFASRTKPEAPPASLDLLGEAARFGLPRVAIGGLDVHTM
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCHHHH
RLAREAGADLLATVHAVFGAGDPAAAVRALQASLGDTAQH
HHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA