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The map label for this gene is phoB [H]

Identifier: 92115368

GI number: 92115368

Start: 3626788

End: 3627477

Strand: Direct

Name: phoB [H]

Synonym: Csal_3253

Alternate gene names: 92115368

Gene position: 3626788-3627477 (Clockwise)

Preceding gene: 92115367

Following gene: 92115369

Centisome position: 98.11

GC content: 64.2

Gene sequence:

>690_bases
ATGACCGCCAAGACCGTGCTCATCGTCGATGACGAAGCGTCCATCCGCGAAATGATCGCGGTGGCGCTGGAAATGGCCGA
CTACCGTGTGCTGGAAGCCGACAACGCTCAGGCGGCACATGCCATGGTGGTCGACGACCAACCCGACCTGTTGCTGCTCG
ACTGGATGATGCCCGGCACCAGCGGCATCGAACTGGCGCGGCGTCTCAAGCGCGATACCGCCACTGCCGAATTGCCGATC
ATCCTGCTCACCGCCAAGAGCGAGGAAGACAACAAGATCCAGGGGCTGGAAGCCGGCGCCGACGACTACATCACCAAGCC
CTTCTCGCCGCGGGAGTTGGTGGCACGCCTCAAGGCCGTGCTGCGCCGCACGACTCCCAAGGGCGTGGAGGACCCGGTGG
AGATCGAGGGCCTGCTCCTCGATCCGGTCAGCCATCGGGTCAGCGCCAACGGCCAGGCACTGGATGTAGGCCCCACCGAG
TATCGGCTCTTGCAGTTCTTCATGACCCATCAGGAGCGGGCCTACACGCGTAGCCAGTTGCTCGATCAGGTCTGGGGTGG
CAATGTCTACGTCGAGGAGCGCACCGTCGACGTGCACATCCGCCGCCTGCGCAAGGCGCTCGGCGAGGCCCATCAGCATC
TCATCCAGACGGTACGCGGCACCGGTTATCGCTTCTCCGCCAAGGACTAG

Upstream 100 bases:

>100_bases
TCGCGTGTCACGAAGTTGTCACATTAGCGCGTTGTAATCGTCATGCTGATGTCATTCATGAGTGATTCGCGCCATCGCGA
TCCGCAAGAGGCCTACGCCC

Downstream 100 bases:

>100_bases
CGCGTGCACTATTGGACCAACGAACTGTGGCGACTGGCGTACCTGGTCGTCGGACTCGGTGCCCTGGGCTGGCTGCTCGG
CGAGCCCGGCTGGGGGCTGG

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTAKTVLIVDDEASIREMIAVALEMADYRVLEADNAQAAHAMVVDDQPDLLLLDWMMPGTSGIELARRLKRDTATAELPI
ILLTAKSEEDNKIQGLEAGADDYITKPFSPRELVARLKAVLRRTTPKGVEDPVEIEGLLLDPVSHRVSANGQALDVGPTE
YRLLQFFMTHQERAYTRSQLLDQVWGGNVYVEERTVDVHIRRLRKALGEAHQHLIQTVRGTGYRFSAKD

Sequences:

>Translated_229_residues
MTAKTVLIVDDEASIREMIAVALEMADYRVLEADNAQAAHAMVVDDQPDLLLLDWMMPGTSGIELARRLKRDTATAELPI
ILLTAKSEEDNKIQGLEAGADDYITKPFSPRELVARLKAVLRRTTPKGVEDPVEIEGLLLDPVSHRVSANGQALDVGPTE
YRLLQFFMTHQERAYTRSQLLDQVWGGNVYVEERTVDVHIRRLRKALGEAHQHLIQTVRGTGYRFSAKD
>Mature_228_residues
TAKTVLIVDDEASIREMIAVALEMADYRVLEADNAQAAHAMVVDDQPDLLLLDWMMPGTSGIELARRLKRDTATAELPII
LLTAKSEEDNKIQGLEAGADDYITKPFSPRELVARLKAVLRRTTPKGVEDPVEIEGLLLDPVSHRVSANGQALDVGPTEY
RLLQFFMTHQERAYTRSQLLDQVWGGNVYVEERTVDVHIRRLRKALGEAHQHLIQTVRGTGYRFSAKD

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=227, Percent_Identity=57.2687224669604, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1788394, Length=225, Percent_Identity=38.6666666666667, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI1786784, Length=225, Percent_Identity=36, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786911, Length=225, Percent_Identity=37.3333333333333, Blast_Score=137, Evalue=4e-34,
Organism=Escherichia coli, GI1789809, Length=228, Percent_Identity=34.6491228070175, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=32.7354260089686, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790863, Length=237, Percent_Identity=33.3333333333333, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1790860, Length=226, Percent_Identity=35.3982300884956, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI2367329, Length=227, Percent_Identity=35.2422907488987, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=30.3571428571429, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1790552, Length=224, Percent_Identity=31.6964285714286, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789402, Length=223, Percent_Identity=28.6995515695067, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787229, Length=229, Percent_Identity=31.4410480349345, Blast_Score=98, Evalue=4e-22,
Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=33.0508474576271, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=35.0877192982456, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1790299, Length=115, Percent_Identity=34.7826086956522, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788191, Length=118, Percent_Identity=29.6610169491525, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 25673; Mature: 25541

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKTVLIVDDEASIREMIAVALEMADYRVLEADNAQAAHAMVVDDQPDLLLLDWMMPGT
CCCEEEEEECCCHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCC
SGIELARRLKRDTATAELPIILLTAKSEEDNKIQGLEAGADDYITKPFSPRELVARLKAV
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LRRTTPKGVEDPVEIEGLLLDPVSHRVSANGQALDVGPTEYRLLQFFMTHQERAYTRSQL
HHHCCCCCCCCCCEECEEEECCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
LDQVWGGNVYVEERTVDVHIRRLRKALGEAHQHLIQTVRGTGYRFSAKD
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
>Mature Secondary Structure 
TAKTVLIVDDEASIREMIAVALEMADYRVLEADNAQAAHAMVVDDQPDLLLLDWMMPGT
CCEEEEEECCCHHHHHHHHHHHHHHCEEEEECCCCCCEEEEEECCCCCEEEEEECCCCC
SGIELARRLKRDTATAELPIILLTAKSEEDNKIQGLEAGADDYITKPFSPRELVARLKAV
CHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LRRTTPKGVEDPVEIEGLLLDPVSHRVSANGQALDVGPTEYRLLQFFMTHQERAYTRSQL
HHHCCCCCCCCCCEECEEEECCHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHH
LDQVWGGNVYVEERTVDVHIRRLRKALGEAHQHLIQTVRGTGYRFSAKD
HHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]