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The map label for this gene is spoT [H]

Identifier: 92115350

GI number: 92115350

Start: 3603996

End: 3606131

Strand: Direct

Name: spoT [H]

Synonym: Csal_3235

Alternate gene names: 92115350

Gene position: 3603996-3606131 (Clockwise)

Preceding gene: 92115349

Following gene: 92115351

Centisome position: 97.49

GC content: 64.65

Gene sequence:

>2136_bases
ATGTTCACCATCGACGATCTGGCAGACCGGCTGGGCGGCTACTTGCCGCCCGAGGAAATACTGCAGGTCAAGCGCGCCTT
CTATTACGCCGAGCAGGCCCATGACGGCCAGCGTCGGCGCTCCGGCGAACCCTACGTCACGCATCCGCTGGCCGTCGCCA
ACATCCTCGCCAACATGCACATGGACCACCAGAGCCTGATGGCCGCCATGCTGCACGACGTCATCGAGGACACCGGCATC
ACCAAGGAGGCGCTGGCGCGTCAGTTCGGCGACCCCGTGGCCGAGCTGGTCGACGGCGTCTCCAAGCTGACCCAGATCAC
CTTCGAGGACAAGGCGGTCGCCCAGGCGGAAAACTTCCAGAAGATGGTCCTGGCGATGTCCAAGGACATCCGCGTGATCA
TCGTCAAGCTCGCCGACCGCCTGCACAACATGCGCACGCTCGGCGCGCTGCGTCCGGACAAGAAACGCCGCATCGCCCGC
GAGACGCTGGAAATCTACGCCCGTATCGCCAGCCGCCTGGGCATCAACACCATTCGCGTCGAGCTCGAGGATCTTTCCTT
CCAGGCGATTCATCCGATGCGCGCCGAGCGCATCAAGCGCGCGGTCACCAAGGCGCGCGGCAATCGTCGCTCGATGATGC
GCCAGATCCAGGACAGCCTGCAGAAATGCCTCGACGACGACGCGCTCGTGGGCAGCGTGGTCGGGCGCCAGAAGCACCTG
CTGTCGATCTATCGCAAGATGCGCGACCAGCGCAAACCCTTCGTCGAGATCATGGACGTCTTCGGGTTTCGCATCATCAC
CGACGACGTGGATACCTGCTATCGCATCCTGGGCGCGGTGCACAACCTCTACAAGCCGGTGCCGGGGCGCTTCAAGGACT
ATATCGCCATCCCCAAGGCCAACGGCTACCAGAGCCTGCACACCACGCTGTTCGGCCCCAACGGCATGCCCATCGAGGTA
CAGATCCGTACCCGCGAGATGGAGGCGATGGCCAACAACGGCATCGCCGCGCACTGGCTCTACAAGGCCGGCCAGACCGA
GCGCCCCATCGGCGCCGGCAGCCATGCCCGGGCCCGTGAGTGGGTGCGCGGCCTGCTCGAGATGCAGCGCCACGCCGGCA
ACTCCCTGGAATTCATCGAACACGTCAAGAACGACCTGTTCCCCGACGATATCTACGTGTTCACGCCCAAGGGCGACATC
ATGGAGCTGCCCGGCGGTGCCACCGCGGTGGACTTCGCCTATACCGTGCATACCGACATCGGCAACAGCTGCATCGCCTG
TCGCATCGACCGTCATCTGGCACCGCTTTCCTCGACGCTGGAAAGCGGCCAGACGCTGGAGATCATCACCGCTCCCGGCG
CTCGTCCCAACCTCGCCTGGCTCAATTTCGTGGTCACCGCCAAGGCACGTTCGGCGATCCGCCATGCGATCAAGCATCAG
CAGCGCACCGAGTCCGTGCAGCTGGGCCGGCGTCTGCTCAACCGTGCGCTGGGCGCCTTCGAGACCAGCCTCGAGGAACT
GCCCGAGCCGCGCATGGCGGCGGTGCTCGACGAGCTGGAACTCAAGCACGAAGATGATCTGCTCGAATCGATCGGGCTCG
GTACCCGCATGGCCTATGCCATCGCCCGGCGCCTGCTGGCCTCCACCGAGCCTGCGAGCGCCGAGGAGGTCGCGACCTCG
CCGCATTCCGGCGGACCAATCGTGATCAACGGTGCCGAGGGCATGGTGATCAGCTTCGCGCGTTGCTGCTATCCGTTGCC
CGGCGATCCGGTCATCGGCCATCTCTCGGTCGGCAAGGGGATCGTGGTGCATCGCCATGACTGTGGTAACCTGCAGGAGC
TGCGCGACGACCCCGACAAGCTGTTCGCCCTGGAGTGGTCGCAGCAGATCGACCAGGACTTTCCGGTGGCGCTGCGTCTC
CACGTCGAGACGCGACGCGGCCTGGTCGCCGAACTGGCCGGCCTGGTGACCGATGCCGATGCCAACATCGAGCGCATCGG
CATCGAAGAACGCGACGCACGCCTGTCGATCGTCAACTTGACGCTGGCGGTCCATGACCGCGTGCATCTGGCAAGTATCA
TGAAACGACTGCGCAACCTGCCCCACGTCGGCAAGATCACCCGCGTTCGCAGTTAA

Upstream 100 bases:

>100_bases
GCAGCATTCTCAACGAGGCCGTCGAACCGCCCGCCGCCAAGCCGCGTCCCGAGCGAGAGTTCAACGACTGAGTTCGCTCA
CTTCTCGACAGGCGCGCGCT

Downstream 100 bases:

>100_bases
CGCCATGGCGACCGATCCGCCGCCCCTGTCGCGGCGGGTCGGTCGTTTCGTTTTGGCGATGCTCGGCGCCCCCGCTCATG
CGGGTGCGGCGGCGTCGCCG

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 711; Mature: 711

Protein sequence:

>711_residues
MFTIDDLADRLGGYLPPEEILQVKRAFYYAEQAHDGQRRRSGEPYVTHPLAVANILANMHMDHQSLMAAMLHDVIEDTGI
TKEALARQFGDPVAELVDGVSKLTQITFEDKAVAQAENFQKMVLAMSKDIRVIIVKLADRLHNMRTLGALRPDKKRRIAR
ETLEIYARIASRLGINTIRVELEDLSFQAIHPMRAERIKRAVTKARGNRRSMMRQIQDSLQKCLDDDALVGSVVGRQKHL
LSIYRKMRDQRKPFVEIMDVFGFRIITDDVDTCYRILGAVHNLYKPVPGRFKDYIAIPKANGYQSLHTTLFGPNGMPIEV
QIRTREMEAMANNGIAAHWLYKAGQTERPIGAGSHARAREWVRGLLEMQRHAGNSLEFIEHVKNDLFPDDIYVFTPKGDI
MELPGGATAVDFAYTVHTDIGNSCIACRIDRHLAPLSSTLESGQTLEIITAPGARPNLAWLNFVVTAKARSAIRHAIKHQ
QRTESVQLGRRLLNRALGAFETSLEELPEPRMAAVLDELELKHEDDLLESIGLGTRMAYAIARRLLASTEPASAEEVATS
PHSGGPIVINGAEGMVISFARCCYPLPGDPVIGHLSVGKGIVVHRHDCGNLQELRDDPDKLFALEWSQQIDQDFPVALRL
HVETRRGLVAELAGLVTDADANIERIGIEERDARLSIVNLTLAVHDRVHLASIMKRLRNLPHVGKITRVRS

Sequences:

>Translated_711_residues
MFTIDDLADRLGGYLPPEEILQVKRAFYYAEQAHDGQRRRSGEPYVTHPLAVANILANMHMDHQSLMAAMLHDVIEDTGI
TKEALARQFGDPVAELVDGVSKLTQITFEDKAVAQAENFQKMVLAMSKDIRVIIVKLADRLHNMRTLGALRPDKKRRIAR
ETLEIYARIASRLGINTIRVELEDLSFQAIHPMRAERIKRAVTKARGNRRSMMRQIQDSLQKCLDDDALVGSVVGRQKHL
LSIYRKMRDQRKPFVEIMDVFGFRIITDDVDTCYRILGAVHNLYKPVPGRFKDYIAIPKANGYQSLHTTLFGPNGMPIEV
QIRTREMEAMANNGIAAHWLYKAGQTERPIGAGSHARAREWVRGLLEMQRHAGNSLEFIEHVKNDLFPDDIYVFTPKGDI
MELPGGATAVDFAYTVHTDIGNSCIACRIDRHLAPLSSTLESGQTLEIITAPGARPNLAWLNFVVTAKARSAIRHAIKHQ
QRTESVQLGRRLLNRALGAFETSLEELPEPRMAAVLDELELKHEDDLLESIGLGTRMAYAIARRLLASTEPASAEEVATS
PHSGGPIVINGAEGMVISFARCCYPLPGDPVIGHLSVGKGIVVHRHDCGNLQELRDDPDKLFALEWSQQIDQDFPVALRL
HVETRRGLVAELAGLVTDADANIERIGIEERDARLSIVNLTLAVHDRVHLASIMKRLRNLPHVGKITRVRS
>Mature_711_residues
MFTIDDLADRLGGYLPPEEILQVKRAFYYAEQAHDGQRRRSGEPYVTHPLAVANILANMHMDHQSLMAAMLHDVIEDTGI
TKEALARQFGDPVAELVDGVSKLTQITFEDKAVAQAENFQKMVLAMSKDIRVIIVKLADRLHNMRTLGALRPDKKRRIAR
ETLEIYARIASRLGINTIRVELEDLSFQAIHPMRAERIKRAVTKARGNRRSMMRQIQDSLQKCLDDDALVGSVVGRQKHL
LSIYRKMRDQRKPFVEIMDVFGFRIITDDVDTCYRILGAVHNLYKPVPGRFKDYIAIPKANGYQSLHTTLFGPNGMPIEV
QIRTREMEAMANNGIAAHWLYKAGQTERPIGAGSHARAREWVRGLLEMQRHAGNSLEFIEHVKNDLFPDDIYVFTPKGDI
MELPGGATAVDFAYTVHTDIGNSCIACRIDRHLAPLSSTLESGQTLEIITAPGARPNLAWLNFVVTAKARSAIRHAIKHQ
QRTESVQLGRRLLNRALGAFETSLEELPEPRMAAVLDELELKHEDDLLESIGLGTRMAYAIARRLLASTEPASAEEVATS
PHSGGPIVINGAEGMVISFARCCYPLPGDPVIGHLSVGKGIVVHRHDCGNLQELRDDPDKLFALEWSQQIDQDFPVALRL
HVETRRGLVAELAGLVTDADANIERIGIEERDARLSIVNLTLAVHDRVHLASIMKRLRNLPHVGKITRVRS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=715, Percent_Identity=52.027972027972, Blast_Score=767, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=681, Percent_Identity=32.0117474302496, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI25143118, Length=140, Percent_Identity=34.2857142857143, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 79548; Mature: 79548

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTIDDLADRLGGYLPPEEILQVKRAFYYAEQAHDGQRRRSGEPYVTHPLAVANILANMH
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEECHHHHHHHHHHHC
MDHQSLMAAMLHDVIEDTGITKEALARQFGDPVAELVDGVSKLTQITFEDKAVAQAENFQ
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KMVLAMSKDIRVIIVKLADRLHNMRTLGALRPDKKRRIARETLEIYARIASRLGINTIRV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
ELEDLSFQAIHPMRAERIKRAVTKARGNRRSMMRQIQDSLQKCLDDDALVGSVVGRQKHL
EEECCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LSIYRKMRDQRKPFVEIMDVFGFRIITDDVDTCYRILGAVHNLYKPVPGRFKDYIAIPKA
HHHHHHHHHHCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
NGYQSLHTTLFGPNGMPIEVQIRTREMEAMANNGIAAHWLYKAGQTERPIGAGSHARARE
CCCHHHHHEEECCCCCEEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHH
WVRGLLEMQRHAGNSLEFIEHVKNDLFPDDIYVFTPKGDIMELPGGATAVDFAYTVHTDI
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCEEEEEEEEECCC
GNSCIACRIDRHLAPLSSTLESGQTLEIITAPGARPNLAWLNFVVTAKARSAIRHAIKHQ
CCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QRTESVQLGRRLLNRALGAFETSLEELPEPRMAAVLDELELKHEDDLLESIGLGTRMAYA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
IARRLLASTEPASAEEVATSPHSGGPIVINGAEGMVISFARCCYPLPGDPVIGHLSVGKG
HHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCC
IVVHRHDCGNLQELRDDPDKLFALEWSQQIDQDFPVALRLHVETRRGLVAELAGLVTDAD
EEEEECCCCCHHHHHCCHHHEEEEHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCC
ANIERIGIEERDARLSIVNLTLAVHDRVHLASIMKRLRNLPHVGKITRVRS
CCHHCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure
MFTIDDLADRLGGYLPPEEILQVKRAFYYAEQAHDGQRRRSGEPYVTHPLAVANILANMH
CCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCEECHHHHHHHHHHHC
MDHQSLMAAMLHDVIEDTGITKEALARQFGDPVAELVDGVSKLTQITFEDKAVAQAENFQ
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
KMVLAMSKDIRVIIVKLADRLHNMRTLGALRPDKKRRIARETLEIYARIASRLGINTIRV
HHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEE
ELEDLSFQAIHPMRAERIKRAVTKARGNRRSMMRQIQDSLQKCLDDDALVGSVVGRQKHL
EEECCCHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LSIYRKMRDQRKPFVEIMDVFGFRIITDDVDTCYRILGAVHNLYKPVPGRFKDYIAIPKA
HHHHHHHHHHCCHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
NGYQSLHTTLFGPNGMPIEVQIRTREMEAMANNGIAAHWLYKAGQTERPIGAGSHARARE
CCCHHHHHEEECCCCCEEEEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHH
WVRGLLEMQRHAGNSLEFIEHVKNDLFPDDIYVFTPKGDIMELPGGATAVDFAYTVHTDI
HHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCEEEEEEEEECCC
GNSCIACRIDRHLAPLSSTLESGQTLEIITAPGARPNLAWLNFVVTAKARSAIRHAIKHQ
CCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QRTESVQLGRRLLNRALGAFETSLEELPEPRMAAVLDELELKHEDDLLESIGLGTRMAYA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHH
IARRLLASTEPASAEEVATSPHSGGPIVINGAEGMVISFARCCYPLPGDPVIGHLSVGKG
HHHHHHHCCCCCCHHHHHCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCEEEEEECCCC
IVVHRHDCGNLQELRDDPDKLFALEWSQQIDQDFPVALRLHVETRRGLVAELAGLVTDAD
EEEEECCCCCHHHHHCCHHHEEEEHHHHHCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCC
ANIERIGIEERDARLSIVNLTLAVHDRVHLASIMKRLRNLPHVGKITRVRS
CCHHCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]