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The map label for this gene is ygbJ [H]

Identifier: 92115293

GI number: 92115293

Start: 3543583

End: 3544515

Strand: Direct

Name: ygbJ [H]

Synonym: Csal_3178

Alternate gene names: 92115293

Gene position: 3543583-3544515 (Clockwise)

Preceding gene: 92115292

Following gene: 92115294

Centisome position: 95.86

GC content: 66.35

Gene sequence:

>933_bases
ATGACACAAGCACGACGCGCGCGGGTGGGGGTCATCGGCCTGGGGTCGATGGGAATGGGCATGGCGACCTCATTGGTCGA
TGCCGGCTTCACGACCTGGGGATGCGACCTGCGACCCGACGCCCGACAGGCATTCGACGCCCACGGTGGCCGGGCGGTCG
CCACGCCCGCCGAGATGGCCGCTCACGTCGATATCGTCGTCAGCGTGGTCGTCAATGCCGAGCAGACTCGCGAGGTCCTG
TTCGGCCCCCAGGGCGTCGCCGACGGCCTGGCGCCCGGCAGCGTCATCGTCTCGTGCGCTACCTGCGCGCCGGAGGCCGT
GGTCGCCCTGGGCGAGGAGCTCGCCACCCGGGGCCTGCGTCTGCTCGATGCGCCGATTTCCGGTGGCGCCGCCAAGGCCG
CACAAGGCGCGCTGACGATCATGGCCTCGGGGCCTGACGATGTCTTCGACAGCGTCGAGGATGTCCTCGACGCCCTCGCC
ACCAAGGTCTTTCGGCTTGGCGACGCGATGGGACAGGGATCGCAGGTCAAGCTGGTCAACCAGCTGTTGGCGGGGGTGCA
CATCGCCGCCGCCGCCGAAGCCATGGCCTATGGCATTCGTGGCGGCTGCGACCCACGGGTGCTGTATGAAGTCATCACCC
AGAGCGCCGGCAACTCATGGATGTTCGAGAATCGCGTGCCGCACATTCTCGAGGACGATTACACGCCGCGTTCGGCAGTG
GACATCTTCGTCAAGGATCTGGGTCTGGTTCACGATACCGCTCACCGCGACAAGTTTCCCCTGCCACTGACCAGTCAGGC
GCTCACCATGTTTACCCAAGCCTCGGCACAAGGCCATGGCGGCGACGATGACGCTGCCGTGATCAAGACGTTTCCGGGCT
TCGGGTTGCCGTCTCTCGCGGCCAACGATGACGAGACAGGGGAGGAGGAATGA

Upstream 100 bases:

>100_bases
TGCGCCAGGCGCCGACGGACCTGCTGGAACGCATCGAGGACACCGGAACCGACACGTCGGCCTGATCAACGGCCGTTTCA
TCAACGACGAGGAACGCGAG

Downstream 100 bases:

>100_bases
CGATGCCCATCATCGGTTGCATTGCCGACGATTTCACCGGCGCCACGGACGTGGCGAGCATGCTGGTCAGTGCCGGCATG
CGCACGCTGCAGACCATCGG

Product: 3-hydroxyisobutyrate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MTQARRARVGVIGLGSMGMGMATSLVDAGFTTWGCDLRPDARQAFDAHGGRAVATPAEMAAHVDIVVSVVVNAEQTREVL
FGPQGVADGLAPGSVIVSCATCAPEAVVALGEELATRGLRLLDAPISGGAAKAAQGALTIMASGPDDVFDSVEDVLDALA
TKVFRLGDAMGQGSQVKLVNQLLAGVHIAAAAEAMAYGIRGGCDPRVLYEVITQSAGNSWMFENRVPHILEDDYTPRSAV
DIFVKDLGLVHDTAHRDKFPLPLTSQALTMFTQASAQGHGGDDDAAVIKTFPGFGLPSLAANDDETGEEE

Sequences:

>Translated_310_residues
MTQARRARVGVIGLGSMGMGMATSLVDAGFTTWGCDLRPDARQAFDAHGGRAVATPAEMAAHVDIVVSVVVNAEQTREVL
FGPQGVADGLAPGSVIVSCATCAPEAVVALGEELATRGLRLLDAPISGGAAKAAQGALTIMASGPDDVFDSVEDVLDALA
TKVFRLGDAMGQGSQVKLVNQLLAGVHIAAAAEAMAYGIRGGCDPRVLYEVITQSAGNSWMFENRVPHILEDDYTPRSAV
DIFVKDLGLVHDTAHRDKFPLPLTSQALTMFTQASAQGHGGDDDAAVIKTFPGFGLPSLAANDDETGEEE
>Mature_309_residues
TQARRARVGVIGLGSMGMGMATSLVDAGFTTWGCDLRPDARQAFDAHGGRAVATPAEMAAHVDIVVSVVVNAEQTREVLF
GPQGVADGLAPGSVIVSCATCAPEAVVALGEELATRGLRLLDAPISGGAAKAAQGALTIMASGPDDVFDSVEDVLDALAT
KVFRLGDAMGQGSQVKLVNQLLAGVHIAAAAEAMAYGIRGGCDPRVLYEVITQSAGNSWMFENRVPHILEDDYTPRSAVD
IFVKDLGLVHDTAHRDKFPLPLTSQALTMFTQASAQGHGGDDDAAVIKTFPGFGLPSLAANDDETGEEE

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=293, Percent_Identity=31.0580204778157, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI40556376, Length=291, Percent_Identity=26.4604810996564, Blast_Score=121, Evalue=9e-28,
Organism=Escherichia coli, GI1789092, Length=298, Percent_Identity=52.3489932885906, Blast_Score=277, Evalue=6e-76,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=32.1428571428571, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1790315, Length=284, Percent_Identity=27.4647887323944, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=27.4647887323944, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=30, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24655230, Length=293, Percent_Identity=29.6928327645051, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI19922568, Length=293, Percent_Identity=29.6928327645051, Blast_Score=137, Evalue=8e-33,
Organism=Drosophila melanogaster, GI28574115, Length=280, Percent_Identity=25.3571428571429, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 32145; Mature: 32013

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQARRARVGVIGLGSMGMGMATSLVDAGFTTWGCDLRPDARQAFDAHGGRAVATPAEMA
CCCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEECCHHHHH
AHVDIVVSVVVNAEQTREVLFGPQGVADGLAPGSVIVSCATCAPEAVVALGEELATRGLR
HHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHEEEHHHCCHHHHHHHHHHHHHCCCE
LLDAPISGGAAKAAQGALTIMASGPDDVFDSVEDVLDALATKVFRLGDAMGQGSQVKLVN
EEECCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLLAGVHIAAAAEAMAYGIRGGCDPRVLYEVITQSAGNSWMFENRVPHILEDDYTPRSAV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCHHHCCCCCCHHHH
DIFVKDLGLVHDTAHRDKFPLPLTSQALTMFTQASAQGHGGDDDAAVIKTFPGFGLPSLA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCCCCCCC
ANDDETGEEE
CCCCCCCCCC
>Mature Secondary Structure 
TQARRARVGVIGLGSMGMGMATSLVDAGFTTWGCDLRPDARQAFDAHGGRAVATPAEMA
CCCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCCEEECCHHHHH
AHVDIVVSVVVNAEQTREVLFGPQGVADGLAPGSVIVSCATCAPEAVVALGEELATRGLR
HHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCHHEEEHHHCCHHHHHHHHHHHHHCCCE
LLDAPISGGAAKAAQGALTIMASGPDDVFDSVEDVLDALATKVFRLGDAMGQGSQVKLVN
EEECCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
QLLAGVHIAAAAEAMAYGIRGGCDPRVLYEVITQSAGNSWMFENRVPHILEDDYTPRSAV
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEECCCCCHHHCCCCCCHHHH
DIFVKDLGLVHDTAHRDKFPLPLTSQALTMFTQASAQGHGGDDDAAVIKTFPGFGLPSLA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHEEECCCCCCCCCC
ANDDETGEEE
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]