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The map label for this gene is lutR [H]

Identifier: 92115292

GI number: 92115292

Start: 3542792

End: 3543547

Strand: Direct

Name: lutR [H]

Synonym: Csal_3177

Alternate gene names: 92115292

Gene position: 3542792-3543547 (Clockwise)

Preceding gene: 92115286

Following gene: 92115293

Centisome position: 95.84

GC content: 66.8

Gene sequence:

>756_bases
GTGATCACCGATCCAGAGACTCGACCGACCCGTGGTACCCAACTCTCGGAGCAGGTCGCACGGCAGCTGACGCACGCCAT
CCATACCGGCGAGATGCGCCCCGGGGAGCGTTTGCCCACCGAGGCGGCGCTCGGCGAACGCTATGGCATCAGCCGCTCGG
TGGTGCGCGAAGCCATTTCCATGCTGCGTAACGCCGGCCTGGTCACATCGCGTCGCGGCAGCGGCAGCTTCGTCGCCGAG
GTCCCCACCGTGGCGCTGCGCCTGGCGATTCCCGACCCATCGCTGCAATCGATCATCCGTCTTCTCGAATTGCGCCGGGC
CCTGGAAGTCGAGGCGGCGCGTCTGGCGGCGCAGCGCCGCACCCCCTCCCAATTGCGCCGTATTCGCAATGCTATGGCCG
ACATCGACGAGGCTGTCGAAGTCGGCTCCGACGGCGTCGATCAGGACATGGCCTTTCACCGCGCCATCGCCGAGGCGTCG
AACAACACGCACTTCACGGCGATCATCGACTTCTACAACCGCTTCCTGCATCACGCCGTTACCGTGACGCGAACCAATGA
GTCGCTGCGCGAAACGTTCATGGCGCAGGTCATCGAAGAGCATCAAGCGATCGTCGACGCCATCGCCCAGGGCGACGAAG
AGGCCGCCGCCCAGGCCGCCGCGCGCCACCTCGAGCATGCCGAGTGGCGCCTGCGCCAGGCGCCGACGGACCTGCTGGAA
CGCATCGAGGACACCGGAACCGACACGTCGGCCTGA

Upstream 100 bases:

>100_bases
GTCTGACAAATCTGTCGGAGCAACACCACCATTAGTCGGAGACGCTCACGCCGATACCGGCGAGAGCCTGCGTCTCGCGA
CAAGACAAGAGGGAGCAGCG

Downstream 100 bases:

>100_bases
TCAACGGCCGTTTCATCAACGACGAGGAACGCGAGATGACACAAGCACGACGCGCGCGGGTGGGGGTCATCGGCCTGGGG
TCGATGGGAATGGGCATGGC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MITDPETRPTRGTQLSEQVARQLTHAIHTGEMRPGERLPTEAALGERYGISRSVVREAISMLRNAGLVTSRRGSGSFVAE
VPTVALRLAIPDPSLQSIIRLLELRRALEVEAARLAAQRRTPSQLRRIRNAMADIDEAVEVGSDGVDQDMAFHRAIAEAS
NNTHFTAIIDFYNRFLHHAVTVTRTNESLRETFMAQVIEEHQAIVDAIAQGDEEAAAQAAARHLEHAEWRLRQAPTDLLE
RIEDTGTDTSA

Sequences:

>Translated_251_residues
MITDPETRPTRGTQLSEQVARQLTHAIHTGEMRPGERLPTEAALGERYGISRSVVREAISMLRNAGLVTSRRGSGSFVAE
VPTVALRLAIPDPSLQSIIRLLELRRALEVEAARLAAQRRTPSQLRRIRNAMADIDEAVEVGSDGVDQDMAFHRAIAEAS
NNTHFTAIIDFYNRFLHHAVTVTRTNESLRETFMAQVIEEHQAIVDAIAQGDEEAAAQAAARHLEHAEWRLRQAPTDLLE
RIEDTGTDTSA
>Mature_251_residues
MITDPETRPTRGTQLSEQVARQLTHAIHTGEMRPGERLPTEAALGERYGISRSVVREAISMLRNAGLVTSRRGSGSFVAE
VPTVALRLAIPDPSLQSIIRLLELRRALEVEAARLAAQRRTPSQLRRIRNAMADIDEAVEVGSDGVDQDMAFHRAIAEAS
NNTHFTAIIDFYNRFLHHAVTVTRTNESLRETFMAQVIEEHQAIVDAIAQGDEEAAAQAAARHLEHAEWRLRQAPTDLLE
RIEDTGTDTSA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790032, Length=223, Percent_Identity=32.2869955156951, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI48994961, Length=215, Percent_Identity=31.1627906976744, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1786303, Length=229, Percent_Identity=32.3144104803493, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790780, Length=204, Percent_Identity=29.9019607843137, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789621, Length=235, Percent_Identity=28.936170212766, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789353, Length=231, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI48994955, Length=224, Percent_Identity=28.125, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082208, Length=241, Percent_Identity=26.9709543568465, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27815; Mature: 27815

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDPETRPTRGTQLSEQVARQLTHAIHTGEMRPGERLPTEAALGERYGISRSVVREAIS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
MLRNAGLVTSRRGSGSFVAEVPTVALRLAIPDPSLQSIIRLLELRRALEVEAARLAAQRR
HHHHCCCCEECCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TPSQLRRIRNAMADIDEAVEVGSDGVDQDMAFHRAIAEASNNTHFTAIIDFYNRFLHHAV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TVTRTNESLRETFMAQVIEEHQAIVDAIAQGDEEAAAQAAARHLEHAEWRLRQAPTDLLE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
RIEDTGTDTSA
HHHHCCCCCCC
>Mature Secondary Structure
MITDPETRPTRGTQLSEQVARQLTHAIHTGEMRPGERLPTEAALGERYGISRSVVREAIS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
MLRNAGLVTSRRGSGSFVAEVPTVALRLAIPDPSLQSIIRLLELRRALEVEAARLAAQRR
HHHHCCCCEECCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
TPSQLRRIRNAMADIDEAVEVGSDGVDQDMAFHRAIAEASNNTHFTAIIDFYNRFLHHAV
CHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
TVTRTNESLRETFMAQVIEEHQAIVDAIAQGDEEAAAQAAARHLEHAEWRLRQAPTDLLE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHH
RIEDTGTDTSA
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]