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The map label for this gene is pcnB [H]

Identifier: 92115178

GI number: 92115178

Start: 3412417

End: 3413838

Strand: Direct

Name: pcnB [H]

Synonym: Csal_3063

Alternate gene names: 92115178

Gene position: 3412417-3413838 (Clockwise)

Preceding gene: 92115177

Following gene: 92115179

Centisome position: 92.31

GC content: 65.26

Gene sequence:

>1422_bases
ATGTTCAAAGGATTTACCCGCTTTCTCCAACGCCCCGGTGAACGTTTGAAGACCCTGTTCGGCGCCCAAGAGGCCTCCGG
CCCTGTCACGCCCAGCCTGCGTGTCATTCCACGGGATCAGCACAACGTGTCTCGCCGTCTGTTCAGCGAGAACGCTCTCA
AGGTTCTGTACCGCCTGCACAACGCCGGACACGAAGCCTATCTGGTCGGCGGCTGCGTGCGCGATTCGCTGCTCGGCAAG
GTGCCCAAGGACTTCGACATCGCCACCGACGCCACGCCGGAGGAAATTCGCACCCTGTTTCGCAACTCGCGCATCATCGG
GCGCCGTTTCCGCATCGTGCATGTGCGCTTCGGTCGCGAAATCATCGAGGTGACGACGTTCCGCGGCCGACCAGGCGACG
ATCACGACGATACACTGTCCCAGCAGTCCGACGATGGACTGCTGCTGCGCGACAATGTCTGGGGCACCATCGAAGAAGAC
GCGCTGCGTCGCGACTTCACGGTCAACGCGCTGTATTACAATATCGCCGACTTCTCGATTCACGACTGGGCCAACGGCGT
CAGCGACATCGACGAGCGAACCCTGCGCCTGATCGGCGACCCCGAGGTGCGTTACCGCGAGGACCCCGTGCGCATGCTGC
GGGCCGTGCGCTTCGCCGCCAAGCTGGACTTCGACATCGAGCCGCGCACCGAGGCCCCGATCGGCGAACTCGCGCCGCTG
CTGTTGCAGATTCCCCCTGCGCGCCTGTTCGAAGAAGTCCTCAAGCTCTTCCTGTCGGGGCATGCGCTGCGCACGTTCGA
ACTGCTGCGCGAGTACGGGCTGTTCGCCATGCTCTTCCCCGAGACCGACGAGACCCTCGCGGAGTACGACTGGGCACAGC
CATTGATCGAACAGGCGCTGATCAACACCGACCGGCGCATCCAGGAAGACCGGCCGGTCACGCCGGCCTTCCTGTACGGT
GCCATGCTGTGGCCCGGCATGCGCCTGCGCGCGGAACGTCTCGAGGCGGAGGGCATGCCCCCCATTCCCGCCCAGCAGAC
CGCATCGCAGCAAAGCATATCGCGGCAGCTGCAGCATACGTCGATTCCCAAGCGCTTCAGCTTGCCGATGCGCGAGATCT
GGGACCTGCAACAGCATCTGCCCAAGCGGCGTGGCAAGCGGGCCTTCCAGACCCTGGAGCACAAGCGCTTTCGTGCCGCG
TACGACTTCCTTCTGCTGCGCGAAACCGCTGGCGAGATCGAGCCCGGCCTGGGCGACTGGTGGACGGCCTTCCAGGATGG
GGACGAGCACGAGCAGGCGCGCTTGATCGCCAAGACGGGCGGCCACCCCGCAGGAACGTCGGACCGCCCCAAGCGCCGAC
CGCGCCGCCGCCGGCGTCGCAACTCGAATGCCGGGCAGCCTAACGACGGCCGCGATGAGTGA

Upstream 100 bases:

>100_bases
CCACCCCGCCTGCTGGCGTTCCATCCTGCGACATGCTTTGCATGCCGAGGAGTGTCGGCTTACACTGGCCCTTTCGCTTT
CCGCGAGTCGTTAACGACGC

Downstream 100 bases:

>100_bases
GCGCGCGCTGCCCGCCTATATCGGGCTGGGCAGCAACCTGGATTCTCCCCGCGCACACGTCACCCGCGCCCTGGACGAAC
TCGACTCCCTGCCGCTGACG

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP; Plasmid copy number protein [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MFKGFTRFLQRPGERLKTLFGAQEASGPVTPSLRVIPRDQHNVSRRLFSENALKVLYRLHNAGHEAYLVGGCVRDSLLGK
VPKDFDIATDATPEEIRTLFRNSRIIGRRFRIVHVRFGREIIEVTTFRGRPGDDHDDTLSQQSDDGLLLRDNVWGTIEED
ALRRDFTVNALYYNIADFSIHDWANGVSDIDERTLRLIGDPEVRYREDPVRMLRAVRFAAKLDFDIEPRTEAPIGELAPL
LLQIPPARLFEEVLKLFLSGHALRTFELLREYGLFAMLFPETDETLAEYDWAQPLIEQALINTDRRIQEDRPVTPAFLYG
AMLWPGMRLRAERLEAEGMPPIPAQQTASQQSISRQLQHTSIPKRFSLPMREIWDLQQHLPKRRGKRAFQTLEHKRFRAA
YDFLLLRETAGEIEPGLGDWWTAFQDGDEHEQARLIAKTGGHPAGTSDRPKRRPRRRRRRNSNAGQPNDGRDE

Sequences:

>Translated_473_residues
MFKGFTRFLQRPGERLKTLFGAQEASGPVTPSLRVIPRDQHNVSRRLFSENALKVLYRLHNAGHEAYLVGGCVRDSLLGK
VPKDFDIATDATPEEIRTLFRNSRIIGRRFRIVHVRFGREIIEVTTFRGRPGDDHDDTLSQQSDDGLLLRDNVWGTIEED
ALRRDFTVNALYYNIADFSIHDWANGVSDIDERTLRLIGDPEVRYREDPVRMLRAVRFAAKLDFDIEPRTEAPIGELAPL
LLQIPPARLFEEVLKLFLSGHALRTFELLREYGLFAMLFPETDETLAEYDWAQPLIEQALINTDRRIQEDRPVTPAFLYG
AMLWPGMRLRAERLEAEGMPPIPAQQTASQQSISRQLQHTSIPKRFSLPMREIWDLQQHLPKRRGKRAFQTLEHKRFRAA
YDFLLLRETAGEIEPGLGDWWTAFQDGDEHEQARLIAKTGGHPAGTSDRPKRRPRRRRRRNSNAGQPNDGRDE
>Mature_473_residues
MFKGFTRFLQRPGERLKTLFGAQEASGPVTPSLRVIPRDQHNVSRRLFSENALKVLYRLHNAGHEAYLVGGCVRDSLLGK
VPKDFDIATDATPEEIRTLFRNSRIIGRRFRIVHVRFGREIIEVTTFRGRPGDDHDDTLSQQSDDGLLLRDNVWGTIEED
ALRRDFTVNALYYNIADFSIHDWANGVSDIDERTLRLIGDPEVRYREDPVRMLRAVRFAAKLDFDIEPRTEAPIGELAPL
LLQIPPARLFEEVLKLFLSGHALRTFELLREYGLFAMLFPETDETLAEYDWAQPLIEQALINTDRRIQEDRPVTPAFLYG
AMLWPGMRLRAERLEAEGMPPIPAQQTASQQSISRQLQHTSIPKRFSLPMREIWDLQQHLPKRRGKRAFQTLEHKRFRAA
YDFLLLRETAGEIEPGLGDWWTAFQDGDEHEQARLIAKTGGHPAGTSDRPKRRPRRRRRRNSNAGQPNDGRDE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's. Seems to be involved in plasmid copy number control [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=255, Percent_Identity=29.4117647058824, Blast_Score=95, Evalue=2e-19,
Organism=Escherichia coli, GI87081691, Length=402, Percent_Identity=53.2338308457711, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1789436, Length=224, Percent_Identity=33.4821428571429, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71995920, Length=231, Percent_Identity=27.7056277056277, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321016, Length=186, Percent_Identity=29.5698924731183, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281360112, Length=301, Percent_Identity=28.9036544850498, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21357337, Length=301, Percent_Identity=28.9036544850498, Blast_Score=89, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 54602; Mature: 54602

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKGFTRFLQRPGERLKTLFGAQEASGPVTPSLRVIPRDQHNVSRRLFSENALKVLYRLH
CCCHHHHHHHCCHHHHHHHHCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
NAGHEAYLVGGCVRDSLLGKVPKDFDIATDATPEEIRTLFRNSRIIGRRFRIVHVRFGRE
HCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCC
IIEVTTFRGRPGDDHDDTLSQQSDDGLLLRDNVWGTIEEDALRRDFTVNALYYNIADFSI
EEHHEEECCCCCCCCCHHHHCCCCCCEEEECCCCCCCHHHHHHHCCEEEEEEEEECCCCH
HDWANGVSDIDERTLRLIGDPEVRYREDPVRMLRAVRFAAKLDFDIEPRTEAPIGELAPL
HHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLQIPPARLFEEVLKLFLSGHALRTFELLREYGLFAMLFPETDETLAEYDWAQPLIEQAL
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
INTDRRIQEDRPVTPAFLYGAMLWPGMRLRAERLEAEGMPPIPAQQTASQQSISRQLQHT
HCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
SIPKRFSLPMREIWDLQQHLPKRRGKRAFQTLEHKRFRAAYDFLLLRETAGEIEPGLGDW
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
WTAFQDGDEHEQARLIAKTGGHPAGTSDRPKRRPRRRRRRNSNAGQPNDGRDE
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MFKGFTRFLQRPGERLKTLFGAQEASGPVTPSLRVIPRDQHNVSRRLFSENALKVLYRLH
CCCHHHHHHHCCHHHHHHHHCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
NAGHEAYLVGGCVRDSLLGKVPKDFDIATDATPEEIRTLFRNSRIIGRRFRIVHVRFGRE
HCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCC
IIEVTTFRGRPGDDHDDTLSQQSDDGLLLRDNVWGTIEEDALRRDFTVNALYYNIADFSI
EEHHEEECCCCCCCCCHHHHCCCCCCEEEECCCCCCCHHHHHHHCCEEEEEEEEECCCCH
HDWANGVSDIDERTLRLIGDPEVRYREDPVRMLRAVRFAAKLDFDIEPRTEAPIGELAPL
HHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
LLQIPPARLFEEVLKLFLSGHALRTFELLREYGLFAMLFPETDETLAEYDWAQPLIEQAL
HHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHH
INTDRRIQEDRPVTPAFLYGAMLWPGMRLRAERLEAEGMPPIPAQQTASQQSISRQLQHT
HCCHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
SIPKRFSLPMREIWDLQQHLPKRRGKRAFQTLEHKRFRAAYDFLLLRETAGEIEPGLGDW
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
WTAFQDGDEHEQARLIAKTGGHPAGTSDRPKRRPRRRRRRNSNAGQPNDGRDE
HHHHCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]