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The map label for this gene is dut [H]

Identifier: 92115098

GI number: 92115098

Start: 3321229

End: 3321705

Strand: Direct

Name: dut [H]

Synonym: Csal_2982

Alternate gene names: 92115098

Gene position: 3321229-3321705 (Clockwise)

Preceding gene: 92115097

Following gene: 92115099

Centisome position: 89.84

GC content: 67.71

Gene sequence:

>477_bases
ATGTCAGAAGCTTCGTTGTCCGCCGACACACGCCTGTCGGTGAAGATACTGGACGAACGCGTACGCGACTTCCCCCTGCC
CAGCTACGCTACCGCCGGCAGTGCCGGCCTGGATCTGCGCGCCCTGCTCGACGGCCCGCTCACCCTGGCGCCCGGCGACT
GCGAGCTGGTACGGACCGGCCTGGCCGTGCACATCGGCGACCCGGGGCTGGCCGGCATGATCCTGCCGCGCTCCGGTCTG
GGGCACAAGCACGGCATCGTGCTGGGCAATCTCGTCGGCCTGATCGATTCCGACTACCAGGGCGAGCTGATGGTGTCGGT
CTGGAACCGGGGACGCGACGCCTTCGTCCTCGAGCCGGGCGAACGCCTGGCACAGTACGTGCTTGTACCTGTGGTACAAG
CGACCCTCGACATCGTCGAGGATTTCGCGGTCAGCGAACGCGCCGCCGGTGGCTTTGGCCATTCCGGCCGCCACTGA

Upstream 100 bases:

>100_bases
CGCAAGCCAAGACCGACCTGGCCGACGCCATCGTCGACCGGGTCTTGAGCCTTTACGCCAACCGCGCCGCCACGGCGCAC
GACTGACGGACCGATTTCCC

Downstream 100 bases:

>100_bases
GTGCGGGCTGGGCGGCCAACGCCCCCGTCCCGATGTTCACGGCCACACGGCCACCGACTTGAAGGGATTCAATCATGAGT
CAGCAAGCTTCCATCAGCGC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MSEASLSADTRLSVKILDERVRDFPLPSYATAGSAGLDLRALLDGPLTLAPGDCELVRTGLAVHIGDPGLAGMILPRSGL
GHKHGIVLGNLVGLIDSDYQGELMVSVWNRGRDAFVLEPGERLAQYVLVPVVQATLDIVEDFAVSERAAGGFGHSGRH

Sequences:

>Translated_158_residues
MSEASLSADTRLSVKILDERVRDFPLPSYATAGSAGLDLRALLDGPLTLAPGDCELVRTGLAVHIGDPGLAGMILPRSGL
GHKHGIVLGNLVGLIDSDYQGELMVSVWNRGRDAFVLEPGERLAQYVLVPVVQATLDIVEDFAVSERAAGGFGHSGRH
>Mature_157_residues
SEASLSADTRLSVKILDERVRDFPLPSYATAGSAGLDLRALLDGPLTLAPGDCELVRTGLAVHIGDPGLAGMILPRSGLG
HKHGIVLGNLVGLIDSDYQGELMVSVWNRGRDAFVLEPGERLAQYVLVPVVQATLDIVEDFAVSERAAGGFGHSGRH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=33.3333333333333, Blast_Score=78, Evalue=4e-15,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=33.3333333333333, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1790071, Length=149, Percent_Identity=69.7986577181208, Blast_Score=221, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI71988561, Length=134, Percent_Identity=39.5522388059701, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319729, Length=151, Percent_Identity=34.4370860927152, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=33.0769230769231, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583610, Length=131, Percent_Identity=33.587786259542, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16682; Mature: 16551

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASLSADTRLSVKILDERVRDFPLPSYATAGSAGLDLRALLDGPLTLAPGDCELVRTG
CCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEECCCCHHEEECC
LAVHIGDPGLAGMILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGRDAFVLEPG
EEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCH
ERLAQYVLVPVVQATLDIVEDFAVSERAAGGFGHSGRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEASLSADTRLSVKILDERVRDFPLPSYATAGSAGLDLRALLDGPLTLAPGDCELVRTG
CCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCEEECCCCHHEEECC
LAVHIGDPGLAGMILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGRDAFVLEPG
EEEEECCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEEECCH
ERLAQYVLVPVVQATLDIVEDFAVSERAAGGFGHSGRH
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA