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The map label for this gene is pncA [H]

Identifier: 92114987

GI number: 92114987

Start: 3201681

End: 3202328

Strand: Direct

Name: pncA [H]

Synonym: Csal_2870

Alternate gene names: 92114987

Gene position: 3201681-3202328 (Clockwise)

Preceding gene: 92114986

Following gene: 92114988

Centisome position: 86.61

GC content: 69.91

Gene sequence:

>648_bases
GTGTTGCAGGATGCCCTCGATATCGTCGACCGCCACTGCGCATTGATCGTCATCGACGTACAGCCCGACTTCATGCCCGG
CGGCCCCCTGGCCTGCGCCGACGGCGACGCCATTCTCCCGGGGCTGACCCAGTTGCTCGGCGAGCGGCGCTTCGCGCATG
TGGTGGCGACCCAGGACTGGCATCCGCCAAGGCACGTCTCCTTCGCCAGCCAGCATGCCGGACACGCCCCCTTCGACACC
CTCGATCTCTACGGCGAGCCGCAGACCCTGTGGCCCGACCATTGCGTCCAGAACACCGCCGGCGCCCAGTTGCACCCCGA
GATCGACTGGGCCCCGGTGGACCTCATCGTGCGCAAGGGCAGCGACCCGCGCGTCGATTCCTACAGCGCCTTTCGCCACA
ACCTGGGCCCGGAGGGCACGCGCCCCACCACCGGCCTGGCCGGCTGGTTGCGCGAACGTGAGGTGGAGACGGTCCATGTC
TGCGGCCTGGCACGCGATGTCTGCGTGCTCTGGAGCGCCGAGGACGCCGTCGCCGCCGGCTTTCGCACGCGCTTCCTCTG
GCCCCTGACGCGTCCCGTCACTCACGACACGGATGCCGCCACGCGGCAGCGTCTGACCCGGCTCGGGGTCGAGATCATCG
GCGCCTGA

Upstream 100 bases:

>100_bases
CGAGTAACGCCGCCATCCCCCGCCGGTCGTCGACGCCCCTTGTCAGGGGCGCCGGCGGCTTCGATACTGAAGGCATCGCC
TCAGGCCAGCGGGAGAGACC

Downstream 100 bases:

>100_bases
GTCATTCCCATCGCACGACAAGCGCAAGGAGAACGCCATGAGCGATCATGTCTACAAGCACATCGAGCTGACCGGTTCCT
CGCCCCACGGCGTCGAGGAT

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MLQDALDIVDRHCALIVIDVQPDFMPGGPLACADGDAILPGLTQLLGERRFAHVVATQDWHPPRHVSFASQHAGHAPFDT
LDLYGEPQTLWPDHCVQNTAGAQLHPEIDWAPVDLIVRKGSDPRVDSYSAFRHNLGPEGTRPTTGLAGWLREREVETVHV
CGLARDVCVLWSAEDAVAAGFRTRFLWPLTRPVTHDTDAATRQRLTRLGVEIIGA

Sequences:

>Translated_215_residues
MLQDALDIVDRHCALIVIDVQPDFMPGGPLACADGDAILPGLTQLLGERRFAHVVATQDWHPPRHVSFASQHAGHAPFDT
LDLYGEPQTLWPDHCVQNTAGAQLHPEIDWAPVDLIVRKGSDPRVDSYSAFRHNLGPEGTRPTTGLAGWLREREVETVHV
CGLARDVCVLWSAEDAVAAGFRTRFLWPLTRPVTHDTDAATRQRLTRLGVEIIGA
>Mature_215_residues
MLQDALDIVDRHCALIVIDVQPDFMPGGPLACADGDAILPGLTQLLGERRFAHVVATQDWHPPRHVSFASQHAGHAPFDT
LDLYGEPQTLWPDHCVQNTAGAQLHPEIDWAPVDLIVRKGSDPRVDSYSAFRHNLGPEGTRPTTGLAGWLREREVETVHV
CGLARDVCVLWSAEDAVAAGFRTRFLWPLTRPVTHDTDAATRQRLTRLGVEIIGA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=183, Percent_Identity=43.7158469945355, Blast_Score=145, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI193206735, Length=225, Percent_Identity=35.1111111111111, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17543130, Length=224, Percent_Identity=34.375, Blast_Score=113, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71999339, Length=223, Percent_Identity=31.8385650224215, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=205, Percent_Identity=32.6829268292683, Blast_Score=102, Evalue=5e-23,
Organism=Drosophila melanogaster, GI24648247, Length=219, Percent_Identity=35.1598173515982, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23669; Mature: 23669

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQDALDIVDRHCALIVIDVQPDFMPGGPLACADGDAILPGLTQLLGERRFAHVVATQDW
CCHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHCHHHEEEEECCCC
HPPRHVSFASQHAGHAPFDTLDLYGEPQTLWPDHCVQNTAGAQLHPEIDWAPVDLIVRKG
CCCCCCCHHHHCCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCEECCCCCCCCEEEEEECC
SDPRVDSYSAFRHNLGPEGTRPTTGLAGWLREREVETVHVCGLARDVCVLWSAEDAVAAG
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHCCCEEEEECCCHHHHHH
FRTRFLWPLTRPVTHDTDAATRQRLTRLGVEIIGA
HHHEEECCCCCCCCCCCHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MLQDALDIVDRHCALIVIDVQPDFMPGGPLACADGDAILPGLTQLLGERRFAHVVATQDW
CCHHHHHHHCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHCHHHEEEEECCCC
HPPRHVSFASQHAGHAPFDTLDLYGEPQTLWPDHCVQNTAGAQLHPEIDWAPVDLIVRKG
CCCCCCCHHHHCCCCCCCCHHCCCCCCCCCCCHHHHCCCCCCEECCCCCCCCEEEEEECC
SDPRVDSYSAFRHNLGPEGTRPTTGLAGWLREREVETVHVCGLARDVCVLWSAEDAVAAG
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHHCCCCEEEEHHHCCCEEEEECCCHHHHHH
FRTRFLWPLTRPVTHDTDAATRQRLTRLGVEIIGA
HHHEEECCCCCCCCCCCHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]