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The map label for this gene is rsmB [H]

Identifier: 92114986

GI number: 92114986

Start: 3200286

End: 3201587

Strand: Direct

Name: rsmB [H]

Synonym: Csal_2869

Alternate gene names: 92114986

Gene position: 3200286-3201587 (Clockwise)

Preceding gene: 92114985

Following gene: 92114987

Centisome position: 86.57

GC content: 71.12

Gene sequence:

>1302_bases
ATGCGTGCCGTATGCGCCAGCGCACTGGCGCCACTTCTCGACGGCAAGGGCTCGCTGAGCGCCCTGGATGCACATCTCGA
CACCGCCAGTCTCGACGGCCGCGATCGTGCCTTCGCCAAGGCCTTGTGCTATGGCGTCTGCCGCACCCTGCCGCGCCTGC
AAGCGTTGGCCAAGCTCCTGCTGCGCCAGCCCTTCAAGCGCCGTGACCTGGACGTGCAGGCACTGTTGTTGATCGGCCTC
TACCAGTTGCTCTACATGCGTGTGCCGGCCCATGCCGCCGTGGGGGAAACCGCCGGTGCCGCCCGCGGGCTCGGCAAGGG
CTGGGCCACGCGCGTGCTCAACGGCTGTCTGCGCCGCTTTCAACGCGACACCCAGACACTCCAGGCACAGGTCGACCGCG
ACCCGAGCGTTGCCCTGCTGCATCCCGAGTGGATGCTGACCGAGTGGCGCCAGGCCTGGCCCGACGACTGGCGAGCCCTG
GCCGAGGCCAACAACTGCCCCGGCCCCATGACGCTGCGCGTCAATGTCCGCCAGGGCGACCGCGACGCCTACCTGGCCCG
TCTGGAAGACGCCGGCCTCTCGGCCTCGCCCACACCCTTCGCACCGCAGGGCCTGACCCTGGCCGAGCCCTGCGATGTGC
GCGCCTTGCCCGGCTTCGCCGAGGGCGCCGTGAGCGTGCAGGACGAAGCCGCCCAACTGGCCGCCACCCTGCTCGCCCCG
TGTCTCGCCGAACGCCCCGGCGCACGCGTGCTGGATGCCTGCAGCGCGCCCGGCGGCAAGACCGCCCACCTTCTCGAAGC
GTTCGATATCGACCTGACCGCGCTGGACAGCGACGCCGAGCGGCTGGACCGGGTCAATGCCACGCTGGAGCGCCTGGGAC
TCGACGCCACGACCATAGCCGCCGATGCCAGCCACCACGCCTGGTGGGACGGCCATGCCTTCGATGCCGTCCTGCTGGAT
GCCCCCTGCTCGGGAACCGGCGTCATCCGCCGGCACCCGGACATCAAGCGCCTGCGGCGTCCGCGCGACATCCGCGAACT
CGCCGCGTTGCAGGCGCGTCTGCTCGACGCCGCGTGGCACACTCTGGCCCCCGGCGGCACCCTGCTGTACGCCACCTGCT
CGGTGATGCCCGAGGAAAATGCCGACCAGGTCGCGGCCTTCCTCGCTCGCACCCCGGACGCTCGCGCCGAGACCATCGAG
GCCGCATGGGGCCGCCCGGCGGGCGACGGGCGTCAGCTGCTGCCCCGCATCGACGGCCACGACGGCTTTTTCTACGCCCG
CTTGCGCAAGCAGTCCGAGTAA

Upstream 100 bases:

>100_bases
GCAAGCCGCTGGCCGTGCGCGACCTGCTCAACGCACGTGGCGAGCGCTTTCCCGAGGGGCTTCGTCTGGGACAGGAGGAT
CGCGCGTGAGCACTCCGGAC

Downstream 100 bases:

>100_bases
CGCCGCCATCCCCCGCCGGTCGTCGACGCCCCTTGTCAGGGGCGCCGGCGGCTTCGATACTGAAGGCATCGCCTCAGGCC
AGCGGGAGAGACCGTGTTGC

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRAVCASALAPLLDGKGSLSALDAHLDTASLDGRDRAFAKALCYGVCRTLPRLQALAKLLLRQPFKRRDLDVQALLLIGL
YQLLYMRVPAHAAVGETAGAARGLGKGWATRVLNGCLRRFQRDTQTLQAQVDRDPSVALLHPEWMLTEWRQAWPDDWRAL
AEANNCPGPMTLRVNVRQGDRDAYLARLEDAGLSASPTPFAPQGLTLAEPCDVRALPGFAEGAVSVQDEAAQLAATLLAP
CLAERPGARVLDACSAPGGKTAHLLEAFDIDLTALDSDAERLDRVNATLERLGLDATTIAADASHHAWWDGHAFDAVLLD
APCSGTGVIRRHPDIKRLRRPRDIRELAALQARLLDAAWHTLAPGGTLLYATCSVMPEENADQVAAFLARTPDARAETIE
AAWGRPAGDGRQLLPRIDGHDGFFYARLRKQSE

Sequences:

>Translated_433_residues
MRAVCASALAPLLDGKGSLSALDAHLDTASLDGRDRAFAKALCYGVCRTLPRLQALAKLLLRQPFKRRDLDVQALLLIGL
YQLLYMRVPAHAAVGETAGAARGLGKGWATRVLNGCLRRFQRDTQTLQAQVDRDPSVALLHPEWMLTEWRQAWPDDWRAL
AEANNCPGPMTLRVNVRQGDRDAYLARLEDAGLSASPTPFAPQGLTLAEPCDVRALPGFAEGAVSVQDEAAQLAATLLAP
CLAERPGARVLDACSAPGGKTAHLLEAFDIDLTALDSDAERLDRVNATLERLGLDATTIAADASHHAWWDGHAFDAVLLD
APCSGTGVIRRHPDIKRLRRPRDIRELAALQARLLDAAWHTLAPGGTLLYATCSVMPEENADQVAAFLARTPDARAETIE
AAWGRPAGDGRQLLPRIDGHDGFFYARLRKQSE
>Mature_433_residues
MRAVCASALAPLLDGKGSLSALDAHLDTASLDGRDRAFAKALCYGVCRTLPRLQALAKLLLRQPFKRRDLDVQALLLIGL
YQLLYMRVPAHAAVGETAGAARGLGKGWATRVLNGCLRRFQRDTQTLQAQVDRDPSVALLHPEWMLTEWRQAWPDDWRAL
AEANNCPGPMTLRVNVRQGDRDAYLARLEDAGLSASPTPFAPQGLTLAEPCDVRALPGFAEGAVSVQDEAAQLAATLLAP
CLAERPGARVLDACSAPGGKTAHLLEAFDIDLTALDSDAERLDRVNATLERLGLDATTIAADASHHAWWDGHAFDAVLLD
APCSGTGVIRRHPDIKRLRRPRDIRELAALQARLLDAAWHTLAPGGTLLYATCSVMPEENADQVAAFLARTPDARAETIE
AAWGRPAGDGRQLLPRIDGHDGFFYARLRKQSE

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=347, Percent_Identity=30.2593659942363, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI76150623, Length=347, Percent_Identity=30.2593659942363, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI32698918, Length=194, Percent_Identity=29.8969072164948, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI8922322, Length=203, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI23199998, Length=203, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI270288818, Length=203, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI270288816, Length=203, Percent_Identity=34.4827586206897, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI39995082, Length=281, Percent_Identity=25.6227758007117, Blast_Score=76, Evalue=8e-14,
Organism=Homo sapiens, GI301336155, Length=182, Percent_Identity=30.2197802197802, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI11545785, Length=165, Percent_Identity=35.1515151515151, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI40316918, Length=181, Percent_Identity=31.4917127071823, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI2367212, Length=433, Percent_Identity=45.2655889145497, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI87081985, Length=315, Percent_Identity=31.7460317460317, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17536757, Length=315, Percent_Identity=29.8412698412698, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25143471, Length=181, Percent_Identity=33.1491712707182, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71998419, Length=178, Percent_Identity=34.2696629213483, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=228, Percent_Identity=30.2631578947368, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=301, Percent_Identity=28.9036544850498, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6319447, Length=185, Percent_Identity=33.5135135135135, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI22024126, Length=227, Percent_Identity=32.15859030837, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24668781, Length=262, Percent_Identity=29.3893129770992, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356513, Length=261, Percent_Identity=29.5019157088123, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920494, Length=221, Percent_Identity=27.1493212669683, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21356579, Length=224, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 46937; Mature: 46937

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVCASALAPLLDGKGSLSALDAHLDTASLDGRDRAFAKALCYGVCRTLPRLQALAKLL
CCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQPFKRRDLDVQALLLIGLYQLLYMRVPAHAAVGETAGAARGLGKGWATRVLNGCLRRF
HHCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QRDTQTLQAQVDRDPSVALLHPEWMLTEWRQAWPDDWRALAEANNCPGPMTLRVNVRQGD
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCC
RDAYLARLEDAGLSASPTPFAPQGLTLAEPCDVRALPGFAEGAVSVQDEAAQLAATLLAP
CHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
CLAERPGARVLDACSAPGGKTAHLLEAFDIDLTALDSDAERLDRVNATLERLGLDATTIA
HHHCCCCCHHHHHHCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCCHHHEE
ADASHHAWWDGHAFDAVLLDAPCSGTGVIRRHPDIKRLRRPRDIRELAALQARLLDAAWH
CCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
TLAPGGTLLYATCSVMPEENADQVAAFLARTPDARAETIEAAWGRPAGDGRQLLPRIDGH
HCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCC
DGFFYARLRKQSE
CCEEEEEHHHCCC
>Mature Secondary Structure
MRAVCASALAPLLDGKGSLSALDAHLDTASLDGRDRAFAKALCYGVCRTLPRLQALAKLL
CCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRQPFKRRDLDVQALLLIGLYQLLYMRVPAHAAVGETAGAARGLGKGWATRVLNGCLRRF
HHCCHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QRDTQTLQAQVDRDPSVALLHPEWMLTEWRQAWPDDWRALAEANNCPGPMTLRVNVRQGD
HHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEEEEECCCC
RDAYLARLEDAGLSASPTPFAPQGLTLAEPCDVRALPGFAEGAVSVQDEAAQLAATLLAP
CHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
CLAERPGARVLDACSAPGGKTAHLLEAFDIDLTALDSDAERLDRVNATLERLGLDATTIA
HHHCCCCCHHHHHHCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHCCCHHHEE
ADASHHAWWDGHAFDAVLLDAPCSGTGVIRRHPDIKRLRRPRDIRELAALQARLLDAAWH
CCCCCCCCCCCCCCCEEEEECCCCCCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHH
TLAPGGTLLYATCSVMPEENADQVAAFLARTPDARAETIEAAWGRPAGDGRQLLPRIDGH
HCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCC
DGFFYARLRKQSE
CCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]