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The map label for this gene is iscA [H]

Identifier: 92114966

GI number: 92114966

Start: 3180515

End: 3180841

Strand: Direct

Name: iscA [H]

Synonym: Csal_2849

Alternate gene names: 92114966

Gene position: 3180515-3180841 (Clockwise)

Preceding gene: 92114965

Following gene: 92114967

Centisome position: 86.04

GC content: 63.0

Gene sequence:

>327_bases
ATGGCTAACATTCGCATCAGCCCCGCCGCCGCCGACCAGATCCAGCACGTGCTCGCCGAGCGCGGCAATGGCCTGGGGCT
ACGCGTGGCCGTCAAGCCCAGCGGCTGCTCCGGGTACAGCTACGTACTCGACATCGCCGACGAGATCGGCGACAACGACG
TGCCGTTCGAAGACCATGGCGTCAAGGTGCTCGTCGACAAGGAAGCCCTGACCATCCTCGACGGCACGGAAGTGGATTAC
GTCAGCGAAGGGCTCAACCGCTACTTCCGTTTCAACAATCCCAACGCCACCGACCAGTGCGGCTGCGGGGAAAGCTTCAA
CGTCTAG

Upstream 100 bases:

>100_bases
GACACCGCGACCGCCGAACTTCGTCACGCACTCGGCGCCCTGCGCCAATGAATCGATGTCGCCGCGCCCACCGCGTGGCG
ACGCTCGCAAGGAGGTCATC

Downstream 100 bases:

>100_bases
CACGTCGCAGGGACGTGCGCCACGTCCCACGACACGCTACAATGGCGCGATTTTTCGACATCCGTCGAGCGTCATGCAAC
GCGTCGCCCCGAGCGGCGCG

Product: HesB/YadR/YfhF

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MANIRISPAAADQIQHVLAERGNGLGLRVAVKPSGCSGYSYVLDIADEIGDNDVPFEDHGVKVLVDKEALTILDGTEVDY
VSEGLNRYFRFNNPNATDQCGCGESFNV

Sequences:

>Translated_108_residues
MANIRISPAAADQIQHVLAERGNGLGLRVAVKPSGCSGYSYVLDIADEIGDNDVPFEDHGVKVLVDKEALTILDGTEVDY
VSEGLNRYFRFNNPNATDQCGCGESFNV
>Mature_107_residues
ANIRISPAAADQIQHVLAERGNGLGLRVAVKPSGCSGYSYVLDIADEIGDNDVPFEDHGVKVLVDKEALTILDGTEVDYV
SEGLNRYFRFNNPNATDQCGCGESFNV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=107, Percent_Identity=41.1214953271028, Blast_Score=98, Evalue=1e-21,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=37.3493975903614, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1788877, Length=106, Percent_Identity=54.7169811320755, Blast_Score=126, Evalue=3e-31,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=46.6666666666667, Blast_Score=110, Evalue=2e-26,
Organism=Escherichia coli, GI1786351, Length=107, Percent_Identity=38.3177570093458, Blast_Score=75, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25146411, Length=107, Percent_Identity=35.5140186915888, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI193209025, Length=107, Percent_Identity=35.5140186915888, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71998452, Length=106, Percent_Identity=33.9622641509434, Blast_Score=65, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323001, Length=107, Percent_Identity=40.1869158878505, Blast_Score=81, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24642263, Length=107, Percent_Identity=42.9906542056075, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24649697, Length=107, Percent_Identity=31.7757009345794, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11648; Mature: 11517

Theoretical pI: Translated: 4.16; Mature: 4.16

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIRISPAAADQIQHVLAERGNGLGLRVAVKPSGCSGYSYVLDIADEIGDNDVPFEDHG
CCCEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHEEEEHHHCCCCCCCCCCCC
VKVLVDKEALTILDGTEVDYVSEGLNRYFRFNNPNATDQCGCGESFNV
EEEEECCCEEEEECCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANIRISPAAADQIQHVLAERGNGLGLRVAVKPSGCSGYSYVLDIADEIGDNDVPFEDHG
CCEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHEEEEHHHCCCCCCCCCCCC
VKVLVDKEALTILDGTEVDYVSEGLNRYFRFNNPNATDQCGCGESFNV
EEEEECCCEEEEECCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA