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The map label for this gene is ilvB [H]

Identifier: 92114957

GI number: 92114957

Start: 3170790

End: 3172427

Strand: Direct

Name: ilvB [H]

Synonym: Csal_2840

Alternate gene names: 92114957

Gene position: 3170790-3172427 (Clockwise)

Preceding gene: 92114956

Following gene: 92114958

Centisome position: 85.77

GC content: 70.33

Gene sequence:

>1638_bases
ATGACACACGAGACATCCGCCTCGTCCTCTACCCAGATCAGCTGCGCCGAGGCCCTGGTCCGGCTGCTGCGTGACCGCTA
CGCCGTGGACACCGTCTTCGGGATCCCCGGCGTGCATACCGTGCCGCTCTATCGCGGCCTGGAGGCAGGCGGCCTGCGTC
ATGTCACCCCGCGCCACGAGCAGGGCGCGGGCTTCATGGCGGACGGCTACGCCCGCGCCAGCGGCAAGCCCGGGGTGTGT
TTCATCATCACCGGCCCGGGCATGACCAACATCGCCACCGCCATGGGCCAGGCACTCGCCGACTCGGTGCCCATGCTGGT
GATTTCCAGCGTCAATCGTCGCGACACCCTGGGACGCGGCCAGGGTCGCCTGCACGAGCTGCCCAGCCAGCAGAACGTGC
TGGCGGGCGTCAGCCGTTTCAGCCACACCCTCCTCGACCCGAGCGCCTTGCCGGAGGTGCTCGCCCGCGCCTTCGCGATG
TTCCAGTCGGCGCGACCCGGGCCGGTGCACATCGAGATTCCCATCGATCTCTTCGAGGCCCCCATCGACCTCCCCGACAC
CGCCTCGCCGACACGACTGTACCGTGCCGGCGCGCATCCCGAAGGCATTGCCCTGGCGGCCGAATGGCTGCGCCAGGCGC
AGGCGCCGCTGGTGCTGCTGGGCGGGGGCTGCGCGGAGGCATCAAAAGCGGCACGCACCCTGGTCGAGCATCTCGACGCA
CCGACCGTGACCACGATCAATGCCAAGGGCGTGCTCGGGCGCGGGCATCCCCTCGACCTCGGCGCCAACGCCACCTGGCC
CGAGGTACGCGCCCTGGCACGCGATGCCGACGTCATCCTCGCCGTGGGCACGGAGCTCGGCGAAACCGATTACGACGTGG
TGTTCGACGACGGCTTCACCTTGACCGGTCGCCTCATCCGCATCGACCTGGAGGCCGAGCAACTGGTGCGCAACCAGGCG
ACGGCCCTGGGGCTGGTCAGCGACGCCCAGGCCGCGCTGGAGGCACTGGCGGCGCATTTCCCCTCGCCGCTCGCGCGCCA
GGGAGCGGCCCGCGTGCGCGCCGTGCACGAGGCGCTGGCTCTGCCGCAACGCCCGGACATGGCACCTTTCGTGCCGCTTT
ACACGGCCCTCGACAAGGCACTGCCGGAGGCCATTCTCGTCGGCGACTCCACGGCGCCGGTCTATGCCGGCAATCACCTC
GTCGAGCGCGCGGCGCCGCGGCGTTACTTCAACGCCTCGACCGGTTACGGAACCCTGGGGTACGGCTTGCCGGCGGCGCT
GGGCGCCCAGCTCGCCGCTCCCGGCACGCCGGTCGTGGCTCTGGTCGGCGACGGCGGCGCGATGTTCACCCTGAGCGAGC
TGGCCACGGCCGTCGACGAAGGCCTGCCGGTCGTCGTGGTGCTCTGGAACAACAGCGGCTATGAGGAAATCCGCCGCTAC
ATGGATGTGCATGGCGTCGCTCGCATCGGCGTCGACCCCACGCCGCCCGACTTCCAGTGCCTGGCCGACGGGTTCGGCCT
CGCCTCGCATCGGGTCGAGTCGCCCATCGCGCTGGATCTCGCGCTCAGCAAGGTCTCGCGCACCTCGCCGACGTTGATCG
AGATCGACGCCGAACGCTGGCAGGCGGCACTCGACTGA

Upstream 100 bases:

>100_bases
AAGCTCTGGGAGCTGGTGGTCATCTTCTTGCTGCGCACCGCCATCACCCTGCCGATCATCGTCGTGATGGGCCATTGGCT
GGTCGCTTAAGGAGCGCTCC

Downstream 100 bases:

>100_bases
GACGCCAACCGCGTCCCGAGAGCCGTCTCGGAAACAACGGTTTTCCAAAACACTACAAGGACAACACCATGGCTTTCTTC
CATCGTCTGCTCGGTCAGGA

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTHETSASSSTQISCAEALVRLLRDRYAVDTVFGIPGVHTVPLYRGLEAGGLRHVTPRHEQGAGFMADGYARASGKPGVC
FIITGPGMTNIATAMGQALADSVPMLVISSVNRRDTLGRGQGRLHELPSQQNVLAGVSRFSHTLLDPSALPEVLARAFAM
FQSARPGPVHIEIPIDLFEAPIDLPDTASPTRLYRAGAHPEGIALAAEWLRQAQAPLVLLGGGCAEASKAARTLVEHLDA
PTVTTINAKGVLGRGHPLDLGANATWPEVRALARDADVILAVGTELGETDYDVVFDDGFTLTGRLIRIDLEAEQLVRNQA
TALGLVSDAQAALEALAAHFPSPLARQGAARVRAVHEALALPQRPDMAPFVPLYTALDKALPEAILVGDSTAPVYAGNHL
VERAAPRRYFNASTGYGTLGYGLPAALGAQLAAPGTPVVALVGDGGAMFTLSELATAVDEGLPVVVVLWNNSGYEEIRRY
MDVHGVARIGVDPTPPDFQCLADGFGLASHRVESPIALDLALSKVSRTSPTLIEIDAERWQAALD

Sequences:

>Translated_545_residues
MTHETSASSSTQISCAEALVRLLRDRYAVDTVFGIPGVHTVPLYRGLEAGGLRHVTPRHEQGAGFMADGYARASGKPGVC
FIITGPGMTNIATAMGQALADSVPMLVISSVNRRDTLGRGQGRLHELPSQQNVLAGVSRFSHTLLDPSALPEVLARAFAM
FQSARPGPVHIEIPIDLFEAPIDLPDTASPTRLYRAGAHPEGIALAAEWLRQAQAPLVLLGGGCAEASKAARTLVEHLDA
PTVTTINAKGVLGRGHPLDLGANATWPEVRALARDADVILAVGTELGETDYDVVFDDGFTLTGRLIRIDLEAEQLVRNQA
TALGLVSDAQAALEALAAHFPSPLARQGAARVRAVHEALALPQRPDMAPFVPLYTALDKALPEAILVGDSTAPVYAGNHL
VERAAPRRYFNASTGYGTLGYGLPAALGAQLAAPGTPVVALVGDGGAMFTLSELATAVDEGLPVVVVLWNNSGYEEIRRY
MDVHGVARIGVDPTPPDFQCLADGFGLASHRVESPIALDLALSKVSRTSPTLIEIDAERWQAALD
>Mature_544_residues
THETSASSSTQISCAEALVRLLRDRYAVDTVFGIPGVHTVPLYRGLEAGGLRHVTPRHEQGAGFMADGYARASGKPGVCF
IITGPGMTNIATAMGQALADSVPMLVISSVNRRDTLGRGQGRLHELPSQQNVLAGVSRFSHTLLDPSALPEVLARAFAMF
QSARPGPVHIEIPIDLFEAPIDLPDTASPTRLYRAGAHPEGIALAAEWLRQAQAPLVLLGGGCAEASKAARTLVEHLDAP
TVTTINAKGVLGRGHPLDLGANATWPEVRALARDADVILAVGTELGETDYDVVFDDGFTLTGRLIRIDLEAEQLVRNQAT
ALGLVSDAQAALEALAAHFPSPLARQGAARVRAVHEALALPQRPDMAPFVPLYTALDKALPEAILVGDSTAPVYAGNHLV
ERAAPRRYFNASTGYGTLGYGLPAALGAQLAAPGTPVVALVGDGGAMFTLSELATAVDEGLPVVVVLWNNSGYEEIRRYM
DVHGVARIGVDPTPPDFQCLADGFGLASHRVESPIALDLALSKVSRTSPTLIEIDAERWQAALD

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=24.5583038869258, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI21361361, Length=507, Percent_Identity=27.6134122287968, Blast_Score=144, Evalue=3e-34,
Organism=Escherichia coli, GI87081685, Length=550, Percent_Identity=30.7272727272727, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=29.8747763864043, Blast_Score=195, Evalue=7e-51,
Organism=Escherichia coli, GI1786717, Length=553, Percent_Identity=28.9330922242315, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1788716, Length=538, Percent_Identity=26.9516728624535, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787096, Length=538, Percent_Identity=24.5353159851301, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17542570, Length=540, Percent_Identity=24.2592592592593, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17531299, Length=489, Percent_Identity=24.7443762781186, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17531301, Length=489, Percent_Identity=24.7443762781186, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=28.8495575221239, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6320816, Length=484, Percent_Identity=26.2396694214876, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=534, Percent_Identity=24.7191011235955, Blast_Score=90, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6321524, Length=501, Percent_Identity=25.1497005988024, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=533, Percent_Identity=24.015009380863, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922626, Length=555, Percent_Identity=25.2252252252252, Blast_Score=144, Evalue=1e-34,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 57470; Mature: 57339

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHETSASSSTQISCAEALVRLLRDRYAVDTVFGIPGVHTVPLYRGLEAGGLRHVTPRHE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCCCCCC
QGAGFMADGYARASGKPGVCFIITGPGMTNIATAMGQALADSVPMLVISSVNRRDTLGRG
CCCCCEECCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEHHCCCCHHCCCCC
QGRLHELPSQQNVLAGVSRFSHTLLDPSALPEVLARAFAMFQSARPGPVHIEIPIDLFEA
CCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHC
PIDLPDTASPTRLYRAGAHPEGIALAAEWLRQAQAPLVLLGGGCAEASKAARTLVEHLDA
CCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCC
PTVTTINAKGVLGRGHPLDLGANATWPEVRALARDADVILAVGTELGETDYDVVFDDGFT
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCE
LTGRLIRIDLEAEQLVRNQATALGLVSDAQAALEALAAHFPSPLARQGAARVRAVHEALA
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
LPQRPDMAPFVPLYTALDKALPEAILVGDSTAPVYAGNHLVERAAPRRYFNASTGYGTLG
CCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEECCCHHHHHCCCCHHCCCCCCCCCCC
YGLPAALGAQLAAPGTPVVALVGDGGAMFTLSELATAVDEGLPVVVVLWNNSGYEEIRRY
CCCHHHHCCHHCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
MDVHGVARIGVDPTPPDFQCLADGFGLASHRVESPIALDLALSKVSRTSPTLIEIDAERW
HHHCCEEEECCCCCCCCHHHHHHCCCHHHHHCCCCEEHHHHHHHHHCCCCCEEEEEHHHH
QAALD
HHCCC
>Mature Secondary Structure 
THETSASSSTQISCAEALVRLLRDRYAVDTVFGIPGVHTVPLYRGLEAGGLRHVTPRHE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCCCCCC
QGAGFMADGYARASGKPGVCFIITGPGMTNIATAMGQALADSVPMLVISSVNRRDTLGRG
CCCCCEECCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEHHCCCCHHCCCCC
QGRLHELPSQQNVLAGVSRFSHTLLDPSALPEVLARAFAMFQSARPGPVHIEIPIDLFEA
CCHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHC
PIDLPDTASPTRLYRAGAHPEGIALAAEWLRQAQAPLVLLGGGCAEASKAARTLVEHLDA
CCCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCC
PTVTTINAKGVLGRGHPLDLGANATWPEVRALARDADVILAVGTELGETDYDVVFDDGFT
CCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCE
LTGRLIRIDLEAEQLVRNQATALGLVSDAQAALEALAAHFPSPLARQGAARVRAVHEALA
EEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
LPQRPDMAPFVPLYTALDKALPEAILVGDSTAPVYAGNHLVERAAPRRYFNASTGYGTLG
CCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCEECCCHHHHHCCCCHHCCCCCCCCCCC
YGLPAALGAQLAAPGTPVVALVGDGGAMFTLSELATAVDEGLPVVVVLWNNSGYEEIRRY
CCCHHHHCCHHCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCEEEEEECCCCHHHHHHH
MDVHGVARIGVDPTPPDFQCLADGFGLASHRVESPIALDLALSKVSRTSPTLIEIDAERW
HHHCCEEEECCCCCCCCHHHHHHCCCHHHHHCCCCEEHHHHHHHHHCCCCCEEEEEHHHH
QAALD
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]