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The map label for this gene is argT [H]

Identifier: 92114910

GI number: 92114910

Start: 3123166

End: 3123933

Strand: Direct

Name: argT [H]

Synonym: Csal_2793

Alternate gene names: 92114910

Gene position: 3123166-3123933 (Clockwise)

Preceding gene: 92114909

Following gene: 92114912

Centisome position: 84.49

GC content: 59.51

Gene sequence:

>768_bases
ATGAAAAAATTGCTGACCATGACCCTTCTCGGCAGCGCGCTGCTGGCCGGTAGTGCCTTTGCCCAGGACACCTTGCGCAT
CGGTGTCGACGTGCCGTACGAGCCCATGGAATACCGTACTGCCGATGGCGAACTCACGGGTTTCGACATCGAGCTGGGCA
ATGCGCTTTGTGCCGAGATCGGGGCACAGTGCGAATGGGTCGTGCAGGCGTGGGATGGCATCATTCCCGGCCTGATGGCA
CGCAAGTACGACGCGATCATGTCGTCGATGACCATCAACGAGGAGCGTAAGCAGACCGTTCTCTTCTCCGATCCGTACTT
CACGCCGCCCTCCGCCTGGTTCACGCCCAGCGACAGCGAGATCGACTTCCCCGCCAAGCAGACGCTCGAAGGCAAGACCA
TCGGGGTTCAGCGCGGCACGTTGCAGGACAACTACGCCACCGACATGTTCGGCGACGTGGCCGATATCAGCCGCTACACC
ACGGCGGACGATCTCGTGCTCGACATGGATGCCGGTCGTGTCGATATCGCCTTCCTCGACTTCCCGGTGGGCAAGTCCAC
GCTGCTGGACAGCGATGAAGGGAGCTACAAGGTCGTCGGTGAAATGATCACCGAGCCCAAGAAGTACTTCGGTGACGGCT
TCGGGATTGCCTTCCGCAAGCGCGATCAGGAACTCGCGGACAAGTTCAACGAGGCCCTGGCCACCCTGAAGGAAAACGGT
ACCTATCAGGAGCTCTACAACAAGTATTTCGCGGAAAACGGCAAGTAA

Upstream 100 bases:

>100_bases
CATGGCGGCAGCGCTTCGCTATGCTGCAAGACGCGAAAGTCAGGTCCGCTCACGAGGCGGTCCCATAACAACACACTGCA
AACCCCGTAGAGAGCTTTCC

Downstream 100 bases:

>100_bases
GCGTTACCGGAATCTTGTGTGTCATCGGGCGGCCTGCGGGCCGCCCGATGCGTTCATGGCGATGCCGATGACTTCATGGG
ACGCGCGAAGCGCGCGATGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-ornithine [Cytoplasm]; ADP; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: LAO-binding protein [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKKLLTMTLLGSALLAGSAFAQDTLRIGVDVPYEPMEYRTADGELTGFDIELGNALCAEIGAQCEWVVQAWDGIIPGLMA
RKYDAIMSSMTINEERKQTVLFSDPYFTPPSAWFTPSDSEIDFPAKQTLEGKTIGVQRGTLQDNYATDMFGDVADISRYT
TADDLVLDMDAGRVDIAFLDFPVGKSTLLDSDEGSYKVVGEMITEPKKYFGDGFGIAFRKRDQELADKFNEALATLKENG
TYQELYNKYFAENGK

Sequences:

>Translated_255_residues
MKKLLTMTLLGSALLAGSAFAQDTLRIGVDVPYEPMEYRTADGELTGFDIELGNALCAEIGAQCEWVVQAWDGIIPGLMA
RKYDAIMSSMTINEERKQTVLFSDPYFTPPSAWFTPSDSEIDFPAKQTLEGKTIGVQRGTLQDNYATDMFGDVADISRYT
TADDLVLDMDAGRVDIAFLDFPVGKSTLLDSDEGSYKVVGEMITEPKKYFGDGFGIAFRKRDQELADKFNEALATLKENG
TYQELYNKYFAENGK
>Mature_255_residues
MKKLLTMTLLGSALLAGSAFAQDTLRIGVDVPYEPMEYRTADGELTGFDIELGNALCAEIGAQCEWVVQAWDGIIPGLMA
RKYDAIMSSMTINEERKQTVLFSDPYFTPPSAWFTPSDSEIDFPAKQTLEGKTIGVQRGTLQDNYATDMFGDVADISRYT
TADDLVLDMDAGRVDIAFLDFPVGKSTLLDSDEGSYKVVGEMITEPKKYFGDGFGIAFRKRDQELADKFNEALATLKENG
TYQELYNKYFAENGK

Specific function: This periplasmic binding protein is involved in an arginine transport system. ArgT and histidine-binding protein J (hisJ) interact with a common membrane-bound receptor, hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=36.8627450980392, Blast_Score=170, Evalue=9e-44,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=37.9912663755458, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1787088, Length=253, Percent_Identity=37.5494071146245, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=36.1111111111111, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1787031, Length=252, Percent_Identity=31.3492063492063, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1788228, Length=260, Percent_Identity=27.6923076923077, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28276; Mature: 28276

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLTMTLLGSALLAGSAFAQDTLRIGVDVPYEPMEYRTADGELTGFDIELGNALCAEI
CCHHHHHHHHHHHHHHCCHHHHCCEEECCCCCCCCCCEECCCCCEEEEEEECCHHHHHHH
GAQCEWVVQAWDGIIPGLMARKYDAIMSSMTINEERKQTVLFSDPYFTPPSAWFTPSDSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
IDFPAKQTLEGKTIGVQRGTLQDNYATDMFGDVADISRYTTADDLVLDMDAGRVDIAFLD
CCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEE
FPVGKSTLLDSDEGSYKVVGEMITEPKKYFGDGFGIAFRKRDQELADKFNEALATLKENG
CCCCCCCEECCCCCCHHHHHHHHCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
TYQELYNKYFAENGK
CHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKLLTMTLLGSALLAGSAFAQDTLRIGVDVPYEPMEYRTADGELTGFDIELGNALCAEI
CCHHHHHHHHHHHHHHCCHHHHCCEEECCCCCCCCCCEECCCCCEEEEEEECCHHHHHHH
GAQCEWVVQAWDGIIPGLMARKYDAIMSSMTINEERKQTVLFSDPYFTPPSAWFTPSDSE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCC
IDFPAKQTLEGKTIGVQRGTLQDNYATDMFGDVADISRYTTADDLVLDMDAGRVDIAFLD
CCCCHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEE
FPVGKSTLLDSDEGSYKVVGEMITEPKKYFGDGFGIAFRKRDQELADKFNEALATLKENG
CCCCCCCEECCCCCCHHHHHHHHCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHCCC
TYQELYNKYFAENGK
CHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-ornithine [Periplasm]; H2O; ATP; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [Periplasm] + H2O = ADP + phosphate + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 3040734; 9298646 [H]