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The map label for this gene is dmlA [H]

Identifier: 92114894

GI number: 92114894

Start: 3107457

End: 3108548

Strand: Direct

Name: dmlA [H]

Synonym: Csal_2776

Alternate gene names: 92114894

Gene position: 3107457-3108548 (Clockwise)

Preceding gene: 92114893

Following gene: 92114895

Centisome position: 84.06

GC content: 64.84

Gene sequence:

>1092_bases
ATGGCCAACGACCAGGCACCCCGCAAGGCTCACAGCATCGCCGTCATTCCCGGTGACGGCATCGGCACCGAAGTCACGCC
GGAAGGCGTTCGCGCCCTGAAGGCCGCGGCCGAACGCTTCAACATCGATCTCACCTTCGAAGACTTCGACTTCGCCAGCT
GCGACTACTATCTCGAGCACGGCCAGATGCTGCCGGATGACTGGAAGGCCAAGCTCTCCCGGTTCGATGCCATCTTCTTC
GGCGCCGTCGGCATGCCCGAGAAGGTCCCCGATCACGTGTCGCTGTGGGGCTCGTTGCTGCAGTTCCGGCGCGAGTTCGA
CCAGTACATCAATCTGCGCCCCTGCAAGCTGATGCCCGGCATCAAGAGCCCGCTGGCCGACCGCCAGCCCGGCGAGATCG
ACTTCTACGTGGTACGCGAGAATACCGAGGGCGAGTATTCGAGCATCGGCGGCAAGATCTTCGAGGGCACGCCCCGCGAG
ACGGTGATGCAGGAAACCGTGATGACGCGCACCGGGGTCGACCGCGTTCTCAAGTACGCTTTCGAGCTCGCTCGGAAGCG
TCCGCGCCGCAAGCTCACCTCGGCCACCAAGTCCAACGGCATCTCGATCACCATGCCGTATTGGGACGAGCGCGTGAAAG
CCATGCACGGCGCGTACCCCGAGGTCGCGGTGGACCAATTCCACATCGACATTCTCGCCGCCAACTTCGTGCTCCATCCC
GACTGGTTCGACGTGGTCGTCGCCAGCAATCTGTTCGGCGACATCCTCTCCGACCTGGGGCCCGCCTGTACCGGTACCAT
CGGCGTTGCCCCCTCGGCCAACATCAACCCCGAAGGCGACTTCCCCAGCCTCTTCGAACCGGTCCACGGCAGTGCCCCGG
ACATCGCCGGTCAGGGCGTCGCCAACCCCATCGGCCAGATCTGGTGCGGCGCGATGATGCTCGATCATCTGGGCCACGAG
GACGCCGGCAAGGCCATACTCGACGCCATCGAGTCGGTTCTCGCCAAGGCCGACCCCGGCGTGCTGACGCCCGATCTCAA
GGGCCAAGGCACGACGCGCGACCTGGCGCAGGCCATCATCGAGGCGATCTAG

Upstream 100 bases:

>100_bases
GGCGTCGAGGGCGGCACGCTGCCCTGCGGACATTACCTCCCCGAAGAAGCACCGGATGCAATCCTTCATCAACTCATCGA
CTTCATGAGGTAAGCCCACC

Downstream 100 bases:

>100_bases
ATGCCGCACCCGAGCACGACTCGCGCCGACGACGCGCATCGCTTAAGGTAGGAAAAGGGCTCGGGGTGGCACGTACACCA
CGAGCCCTGACCGTGAACGA

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MANDQAPRKAHSIAVIPGDGIGTEVTPEGVRALKAAAERFNIDLTFEDFDFASCDYYLEHGQMLPDDWKAKLSRFDAIFF
GAVGMPEKVPDHVSLWGSLLQFRREFDQYINLRPCKLMPGIKSPLADRQPGEIDFYVVRENTEGEYSSIGGKIFEGTPRE
TVMQETVMTRTGVDRVLKYAFELARKRPRRKLTSATKSNGISITMPYWDERVKAMHGAYPEVAVDQFHIDILAANFVLHP
DWFDVVVASNLFGDILSDLGPACTGTIGVAPSANINPEGDFPSLFEPVHGSAPDIAGQGVANPIGQIWCGAMMLDHLGHE
DAGKAILDAIESVLAKADPGVLTPDLKGQGTTRDLAQAIIEAI

Sequences:

>Translated_363_residues
MANDQAPRKAHSIAVIPGDGIGTEVTPEGVRALKAAAERFNIDLTFEDFDFASCDYYLEHGQMLPDDWKAKLSRFDAIFF
GAVGMPEKVPDHVSLWGSLLQFRREFDQYINLRPCKLMPGIKSPLADRQPGEIDFYVVRENTEGEYSSIGGKIFEGTPRE
TVMQETVMTRTGVDRVLKYAFELARKRPRRKLTSATKSNGISITMPYWDERVKAMHGAYPEVAVDQFHIDILAANFVLHP
DWFDVVVASNLFGDILSDLGPACTGTIGVAPSANINPEGDFPSLFEPVHGSAPDIAGQGVANPIGQIWCGAMMLDHLGHE
DAGKAILDAIESVLAKADPGVLTPDLKGQGTTRDLAQAIIEAI
>Mature_362_residues
ANDQAPRKAHSIAVIPGDGIGTEVTPEGVRALKAAAERFNIDLTFEDFDFASCDYYLEHGQMLPDDWKAKLSRFDAIFFG
AVGMPEKVPDHVSLWGSLLQFRREFDQYINLRPCKLMPGIKSPLADRQPGEIDFYVVRENTEGEYSSIGGKIFEGTPRET
VMQETVMTRTGVDRVLKYAFELARKRPRRKLTSATKSNGISITMPYWDERVKAMHGAYPEVAVDQFHIDILAANFVLHPD
WFDVVVASNLFGDILSDLGPACTGTIGVAPSANINPEGDFPSLFEPVHGSAPDIAGQGVANPIGQIWCGAMMLDHLGHED
AGKAILDAIESVLAKADPGVLTPDLKGQGTTRDLAQAIIEAI

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=354, Percent_Identity=31.3559322033898, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4758582, Length=358, Percent_Identity=28.7709497206704, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI28178838, Length=332, Percent_Identity=27.710843373494, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178816, Length=353, Percent_Identity=25.4957507082153, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178821, Length=350, Percent_Identity=25.4285714285714, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI28178819, Length=196, Percent_Identity=29.5918367346939, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=67.2222222222222, Blast_Score=503, Evalue=1e-143,
Organism=Escherichia coli, GI87081683, Length=355, Percent_Identity=38.5915492957746, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787381, Length=365, Percent_Identity=26.5753424657534, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71986051, Length=257, Percent_Identity=37.3540856031128, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI17550882, Length=352, Percent_Identity=30.6818181818182, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=27.0949720670391, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=354, Percent_Identity=26.271186440678, Blast_Score=119, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322097, Length=375, Percent_Identity=34.1333333333333, Blast_Score=184, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=356, Percent_Identity=32.5842696629214, Blast_Score=159, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6319830, Length=371, Percent_Identity=32.8840970350404, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=356, Percent_Identity=30.0561797752809, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24643268, Length=354, Percent_Identity=32.2033898305085, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24643270, Length=354, Percent_Identity=32.2033898305085, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24661184, Length=360, Percent_Identity=30, Blast_Score=152, Evalue=3e-37,
Organism=Drosophila melanogaster, GI161078635, Length=354, Percent_Identity=27.9661016949153, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078637, Length=354, Percent_Identity=27.9661016949153, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI161078633, Length=354, Percent_Identity=27.9661016949153, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24650122, Length=354, Percent_Identity=27.9661016949153, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI161078639, Length=265, Percent_Identity=30.9433962264151, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI281362242, Length=365, Percent_Identity=28.7671232876712, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648872, Length=365, Percent_Identity=28.7671232876712, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI20130355, Length=265, Percent_Identity=26.4150943396226, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45550436, Length=271, Percent_Identity=23.2472324723247, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 39702; Mature: 39571

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00059 ADH_ZINC ; PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDQAPRKAHSIAVIPGDGIGTEVTPEGVRALKAAAERFNIDLTFEDFDFASCDYYLEH
CCCCCCCCHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHC
GQMLPDDWKAKLSRFDAIFFGAVGMPEKVPDHVSLWGSLLQFRREFDQYINLRPCKLMPG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IKSPLADRQPGEIDFYVVRENTEGEYSSIGGKIFEGTPRETVMQETVMTRTGVDRVLKYA
CCCCCCCCCCCCEEEEEEECCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
FELARKRPRRKLTSATKSNGISITMPYWDERVKAMHGAYPEVAVDQFHIDILAANFVLHP
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHEEEEEEEEECC
DWFDVVVASNLFGDILSDLGPACTGTIGVAPSANINPEGDFPSLFEPVHGSAPDIAGQGV
CHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
ANPIGQIWCGAMMLDHLGHEDAGKAILDAIESVLAKADPGVLTPDLKGQGTTRDLAQAII
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
EAI
HHC
>Mature Secondary Structure 
ANDQAPRKAHSIAVIPGDGIGTEVTPEGVRALKAAAERFNIDLTFEDFDFASCDYYLEH
CCCCCCCHHCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHC
GQMLPDDWKAKLSRFDAIFFGAVGMPEKVPDHVSLWGSLLQFRREFDQYINLRPCKLMPG
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
IKSPLADRQPGEIDFYVVRENTEGEYSSIGGKIFEGTPRETVMQETVMTRTGVDRVLKYA
CCCCCCCCCCCCEEEEEEECCCCCCHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHH
FELARKRPRRKLTSATKSNGISITMPYWDERVKAMHGAYPEVAVDQFHIDILAANFVLHP
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCHHHHHHHHEEEEEEEEECC
DWFDVVVASNLFGDILSDLGPACTGTIGVAPSANINPEGDFPSLFEPVHGSAPDIAGQGV
CHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
ANPIGQIWCGAMMLDHLGHEDAGKAILDAIESVLAKADPGVLTPDLKGQGTTRDLAQAII
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
EAI
HHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]