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The map label for this gene is mleN [H]

Identifier: 92114827

GI number: 92114827

Start: 3036301

End: 3037743

Strand: Direct

Name: mleN [H]

Synonym: Csal_2709

Alternate gene names: 92114827

Gene position: 3036301-3037743 (Clockwise)

Preceding gene: 92114826

Following gene: 92114828

Centisome position: 82.14

GC content: 63.2

Gene sequence:

>1443_bases
ATGTCCTCACCTCAAGGCCCCACTAGGCTACCCGGCATGATGGAGGTGCTCGCCGTCATGGTGGGCTTCATCGCCATCAT
GTTCCTCAGCATCAACGTACTCGGCCTTCCCATCCAGCTTGCGCTGTTCGCCTCCTGGTTCCTGGTCATGGCACTCGGCC
TCAAGCTCAAGATGGCCTATGCCGACATGCAGCGAGGCCTCATCGACGGCATTCACAATGGCATGGAGGCGGTACTGGTG
CTGACCACCGTCGGTGCGCTGATCGGCACCTGGATCGCCGGCGGCATCGTGCCCAGCATCATCTATTACGGGCTGACGGT
CATGAACCCGCAGATCTTCCTGTTCGCCGCGTTCGTGATCTGCGCCCTGACCTCGCTATCGACCGGCACCTCCTTCGGGA
CCGCGGGCACCGCCGGGGTGGCCATGATGGGCATCGGCCACAGCTTCGGCATTCCGCTTCCGCTGGTGGCCGGCGCGGTC
ATCTCCGGCGCTTACGTCGGCGACAAGATGTCGCCGCTGTCCGATACCACGGTGATGACCGCCTCGCTGTGCAAGGTCAA
CCTGATCGACCACATCCGTTCCATGATGACGGTCAGCGCGCTCGCCATTCCCATCGCCTCCATTCTGTTTCTGATCGTCG
GCTTCTTCTATGTGAGCGGTGACGTGGACACCTCGCGCGCCACCGTCGCCATGCAAGCGCTCGAGGCCAACTTCACCATC
AGCCCCTGGCTGCTGCTGCCGGCGGCGATCGTGATCGGGTTGCTGACGCGCCGCTACCCGGCCATTCCGGTCATCACCTT
CGGCGCGGTGCTGGGCAGCATCTGTGCCTGGTGGGCACAAGGTGTCGGCCCCATCGATGCCATCCGCATCGCCTACGTGG
GCAACGCCATGCAGTCCGACGTGGAGTTCCTGAACACCCTGCTCAATCGCGGCGGCATCGAGTCCATGCTCGGCGTCGTG
GCGCTGATCCTGTTTGCCCTGGGGCTCGGCGGTTTGATGAGCCAGGTGGGCATCCTCAAGGCGATCAGCAATGGCTTTCT
GCGCTGGGCCAACAATCCGGGCCGCTTGACGGTGTCGACCATGCTGGGCGGCTTCTTCGGCAACTTCTTCGGTGGCGCGG
CCTATGTCTCGCTGATTACCGCCAGCACCATCACCGAGAAGAACTATGACGATCAGGGCGTGGATCGGCGAGTGCTCTCG
CGCAATGCCGAAGCCGGCGGCACCATCACCACCCCCATGGTGCCCTGGACCGACGGCGGTGTCTTCATGGCCACCACCCT
CGGCGTCTCGACCATGGCCTATCTTCCCTTCCTCTGGTACCACATGCTGGTGATCGCGATCACGATCCTTTACGGCTACG
CCAACATCGCCATCTGGCGGACCCAGCCCAAGGCGGATGGTCAGGATAACGCCAACCCACGGCAGAACTTCCAGGCCTCC
TAG

Upstream 100 bases:

>100_bases
TCTGTCCGTGACCGAGCACTTCCCGAGCTGGCGCCTTACCGGCTCGATCGCTTCTAGACGCAATCCCCTCAACTCAGACA
ACAAGACCGAAGGAGATCTT

Downstream 100 bases:

>100_bases
CGCGCCGCCAGGAGTACCTGCGTCATCCGACAAGCCCTGCATCCTGCAGGGCTTGTTCGGTTGCAAGGGACTTTCCACGA
CCCGGTCATTGGCTCATGGG

Product: Na+/H+ antiporter NhaC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MSSPQGPTRLPGMMEVLAVMVGFIAIMFLSINVLGLPIQLALFASWFLVMALGLKLKMAYADMQRGLIDGIHNGMEAVLV
LTTVGALIGTWIAGGIVPSIIYYGLTVMNPQIFLFAAFVICALTSLSTGTSFGTAGTAGVAMMGIGHSFGIPLPLVAGAV
ISGAYVGDKMSPLSDTTVMTASLCKVNLIDHIRSMMTVSALAIPIASILFLIVGFFYVSGDVDTSRATVAMQALEANFTI
SPWLLLPAAIVIGLLTRRYPAIPVITFGAVLGSICAWWAQGVGPIDAIRIAYVGNAMQSDVEFLNTLLNRGGIESMLGVV
ALILFALGLGGLMSQVGILKAISNGFLRWANNPGRLTVSTMLGGFFGNFFGGAAYVSLITASTITEKNYDDQGVDRRVLS
RNAEAGGTITTPMVPWTDGGVFMATTLGVSTMAYLPFLWYHMLVIAITILYGYANIAIWRTQPKADGQDNANPRQNFQAS

Sequences:

>Translated_480_residues
MSSPQGPTRLPGMMEVLAVMVGFIAIMFLSINVLGLPIQLALFASWFLVMALGLKLKMAYADMQRGLIDGIHNGMEAVLV
LTTVGALIGTWIAGGIVPSIIYYGLTVMNPQIFLFAAFVICALTSLSTGTSFGTAGTAGVAMMGIGHSFGIPLPLVAGAV
ISGAYVGDKMSPLSDTTVMTASLCKVNLIDHIRSMMTVSALAIPIASILFLIVGFFYVSGDVDTSRATVAMQALEANFTI
SPWLLLPAAIVIGLLTRRYPAIPVITFGAVLGSICAWWAQGVGPIDAIRIAYVGNAMQSDVEFLNTLLNRGGIESMLGVV
ALILFALGLGGLMSQVGILKAISNGFLRWANNPGRLTVSTMLGGFFGNFFGGAAYVSLITASTITEKNYDDQGVDRRVLS
RNAEAGGTITTPMVPWTDGGVFMATTLGVSTMAYLPFLWYHMLVIAITILYGYANIAIWRTQPKADGQDNANPRQNFQAS
>Mature_479_residues
SSPQGPTRLPGMMEVLAVMVGFIAIMFLSINVLGLPIQLALFASWFLVMALGLKLKMAYADMQRGLIDGIHNGMEAVLVL
TTVGALIGTWIAGGIVPSIIYYGLTVMNPQIFLFAAFVICALTSLSTGTSFGTAGTAGVAMMGIGHSFGIPLPLVAGAVI
SGAYVGDKMSPLSDTTVMTASLCKVNLIDHIRSMMTVSALAIPIASILFLIVGFFYVSGDVDTSRATVAMQALEANFTIS
PWLLLPAAIVIGLLTRRYPAIPVITFGAVLGSICAWWAQGVGPIDAIRIAYVGNAMQSDVEFLNTLLNRGGIESMLGVVA
LILFALGLGGLMSQVGILKAISNGFLRWANNPGRLTVSTMLGGFFGNFFGGAAYVSLITASTITEKNYDDQGVDRRVLSR
NAEAGGTITTPMVPWTDGGVFMATTLGVSTMAYLPFLWYHMLVIAITILYGYANIAIWRTQPKADGQDNANPRQNFQAS

Specific function: Couples proton uptake and Na(+) efflux to the substrate- product malate/lactate antiport, in an electroneutral malate- 2H(+)/Na(+)-lactate exchange. Plays a role in supporting growth to high density on malate at reduced protonmotive force [H]

COG id: COG1757

COG function: function code C; Na+/H+ antiporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaC Na(+)/H(+) antiporter family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004770
- InterPro:   IPR018461 [H]

Pfam domain/function: PF03553 Na_H_antiporter [H]

EC number: NA

Molecular weight: Translated: 50799; Mature: 50668

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPQGPTRLPGMMEVLAVMVGFIAIMFLSINVLGLPIQLALFASWFLVMALGLKLKMAY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADMQRGLIDGIHNGMEAVLVLTTVGALIGTWIAGGIVPSIIYYGLTVMNPQIFLFAAFVI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
CALTSLSTGTSFGTAGTAGVAMMGIGHSFGIPLPLVAGAVISGAYVGDKMSPLSDTTVMT
HHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHH
ASLCKVNLIDHIRSMMTVSALAIPIASILFLIVGFFYVSGDVDTSRATVAMQALEANFTI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCC
SPWLLLPAAIVIGLLTRRYPAIPVITFGAVLGSICAWWAQGVGPIDAIRIAYVGNAMQSD
CHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHH
VEFLNTLLNRGGIESMLGVVALILFALGLGGLMSQVGILKAISNGFLRWANNPGRLTVST
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEHHH
MLGGFFGNFFGGAAYVSLITASTITEKNYDDQGVDRRVLSRNAEAGGTITTPMVPWTDGG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEECCCCCCCCCC
VFMATTLGVSTMAYLPFLWYHMLVIAITILYGYANIAIWRTQPKADGQDNANPRQNFQAS
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSPQGPTRLPGMMEVLAVMVGFIAIMFLSINVLGLPIQLALFASWFLVMALGLKLKMAY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10903309 [H]