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The map label for this gene is fucA [C]

Identifier: 92114579

GI number: 92114579

Start: 2768386

End: 2769120

Strand: Direct

Name: fucA [C]

Synonym: Csal_2461

Alternate gene names: 92114579

Gene position: 2768386-2769120 (Clockwise)

Preceding gene: 92114578

Following gene: 92114580

Centisome position: 74.89

GC content: 67.89

Gene sequence:

>735_bases
ATGACGACCGCTACCGAGCTTCCCGGTACTCCCCGCGAACAGCTGGCCTGCGCGTTCCGCTGGCTGGCCCGCCTCGACAT
GCACGAAGGCGTCGCCAATCACTGCAGCCTGGCCACCCAGGCGGACGGCAGGCGCTTTCTGGTCAACCCCGAAGGGCGTC
ATTTCGCGGAGATGCGTGCCAGCGACCTCGTCGAGGTGGATGCCAGCGCCGCACAACGTCCCGAATCCATCGACCCCACC
GCCTGGGCCATTCACGGCGCCATGCACCGCCAGGGCCGCGGCGTGGGATGCGTGATGCATGCCCACTCGCTCTACGCCAC
CGCCCTGGCCTGCCTGGAGACGCCGGAGCTGCCGCCGGTGGAACAGAACGCCATGCGCTTCTTCGAGCGGGTGCACATCG
ATGCCGGATTCGATGGCATGGGGCTCGACGAAGAAGCGGAACGCCTCGGCAAGCTGGCCGGCACGGCGCCGATCCTCCTG
CTCGGCAATCATGGCGTGGTCGCCACCGGCCAGGACGTCGCCGAAGCCTTCGACCACCTCTACTACTTCGAGCGTGCCTG
CCGCTCCTATCTGGTGGCCCGTGCCAGCGGCCTTCCCGTGCGTCCCGTCGCGCCCGAGATCGCACGCAAGACCGCCCGGC
AATGGGAGGATTATCTGGCGCAAGGCTCGGCGCACATGCACCTTGCCGCCTTGATGCGCCTGCTCGATCGCGACGCCCCC
GATTACCGTGATTGA

Upstream 100 bases:

>100_bases
GCGACTACGCGCTGGCCGGCATGCACAAATACATGGCGCTCAAGACGCGCTGGATCCACTATTGATGGCCTCGGTTTCGC
CATCGCCACAGGAGCGAGAC

Downstream 100 bases:

>100_bases
CACGCGACGTCCGACGCCCGGGCCGGAGCGCATCGCGATCCTGCTGATGCCGCAGTTTTCCTTTGCCGGTCTGGGCGCGA
TTCTGGATCCGCTGTTCATC

Product: hypothetical protein

Products: Dihydroxyacetone phosphate; L-Lactaldehyde [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTTATELPGTPREQLACAFRWLARLDMHEGVANHCSLATQADGRRFLVNPEGRHFAEMRASDLVEVDASAAQRPESIDPT
AWAIHGAMHRQGRGVGCVMHAHSLYATALACLETPELPPVEQNAMRFFERVHIDAGFDGMGLDEEAERLGKLAGTAPILL
LGNHGVVATGQDVAEAFDHLYYFERACRSYLVARASGLPVRPVAPEIARKTARQWEDYLAQGSAHMHLAALMRLLDRDAP
DYRD

Sequences:

>Translated_244_residues
MTTATELPGTPREQLACAFRWLARLDMHEGVANHCSLATQADGRRFLVNPEGRHFAEMRASDLVEVDASAAQRPESIDPT
AWAIHGAMHRQGRGVGCVMHAHSLYATALACLETPELPPVEQNAMRFFERVHIDAGFDGMGLDEEAERLGKLAGTAPILL
LGNHGVVATGQDVAEAFDHLYYFERACRSYLVARASGLPVRPVAPEIARKTARQWEDYLAQGSAHMHLAALMRLLDRDAP
DYRD
>Mature_243_residues
TTATELPGTPREQLACAFRWLARLDMHEGVANHCSLATQADGRRFLVNPEGRHFAEMRASDLVEVDASAAQRPESIDPTA
WAIHGAMHRQGRGVGCVMHAHSLYATALACLETPELPPVEQNAMRFFERVHIDAGFDGMGLDEEAERLGKLAGTAPILLL
GNHGVVATGQDVAEAFDHLYYFERACRSYLVARASGLPVRPVAPEIARKTARQWEDYLAQGSAHMHLAALMRLLDRDAPD
YRD

Specific function: Fucose metabolism; third step. [C]

COG id: COG0235

COG function: function code G; Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldolase class II family [H]

Homologues:

Organism=Homo sapiens, GI89276787, Length=193, Percent_Identity=27.979274611399, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI297307160, Length=193, Percent_Identity=27.979274611399, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI9257190, Length=194, Percent_Identity=27.8350515463918, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI297307158, Length=194, Percent_Identity=27.8350515463918, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI9257192, Length=194, Percent_Identity=27.8350515463918, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI29826325, Length=244, Percent_Identity=25.4098360655738, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI29826323, Length=244, Percent_Identity=25.4098360655738, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI9951927, Length=192, Percent_Identity=28.125, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI62912453, Length=192, Percent_Identity=28.125, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI9943848, Length=192, Percent_Identity=28.125, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI29826321, Length=243, Percent_Identity=25.5144032921811, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI29826319, Length=243, Percent_Identity=25.5144032921811, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115533216, Length=192, Percent_Identity=26.5625, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI115533218, Length=192, Percent_Identity=26.5625, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303 [H]

Pfam domain/function: PF00596 Aldolase_II [H]

EC number: 4.1.2.17 [C]

Molecular weight: Translated: 26882; Mature: 26751

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATELPGTPREQLACAFRWLARLDMHEGVANHCSLATQADGRRFLVNPEGRHFAEMRA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCEEEECCCCCHHHHHHH
SDLVEVDASAAQRPESIDPTAWAIHGAMHRQGRGVGCVMHAHSLYATALACLETPELPPV
HHHHHHCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCC
EQNAMRFFERVHIDAGFDGMGLDEEAERLGKLAGTAPILLLGNHGVVATGQDVAEAFDHL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCEEECCHHHHHHHHHH
YYFERACRSYLVARASGLPVRPVAPEIARKTARQWEDYLAQGSAHMHLAALMRLLDRDAP
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
DYRD
CCCC
>Mature Secondary Structure 
TTATELPGTPREQLACAFRWLARLDMHEGVANHCSLATQADGRRFLVNPEGRHFAEMRA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCEEEECCCCCHHHHHHH
SDLVEVDASAAQRPESIDPTAWAIHGAMHRQGRGVGCVMHAHSLYATALACLETPELPPV
HHHHHHCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCC
EQNAMRFFERVHIDAGFDGMGLDEEAERLGKLAGTAPILLLGNHGVVATGQDVAEAFDHL
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCEEECCHHHHHHHHHH
YYFERACRSYLVARASGLPVRPVAPEIARKTARQWEDYLAQGSAHMHLAALMRLLDRDAP
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
DYRD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Co2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 19.3 [C]

Specific activity: 21.0

Km value (mM): 0.7 {L-fuculose} [C]

Substrates: L-Fuculose 1-phosphate [C]

Specific reaction: L-Fuculose 1-phosphate <==> Dihydroxyacetone phosphate + L-Lactaldehyde [C]

General reaction: Elimination of an aldehyde C-C bond; Cleavage [C]

Inhibitor: EDTA; Phosphoglycolohydroxamate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]