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The map label for this gene is aceA [H]

Identifier: 92114567

GI number: 92114567

Start: 2754022

End: 2755332

Strand: Direct

Name: aceA [H]

Synonym: Csal_2449

Alternate gene names: 92114567

Gene position: 2754022-2755332 (Clockwise)

Preceding gene: 92114566

Following gene: 92114572

Centisome position: 74.5

GC content: 63.46

Gene sequence:

>1311_bases
ATGTCCCAGACACGCGAACAGCAAATCGCTGCGTTGGAAAAGGATTGGAACGAGAATCCGCGCTGGAAGGACGTCAAGCG
TCCGTACAGTGCCGAAGATGTGGTTCGACTTCGCGGCAGCGTCAACGAAGCGCACACGCTGGCCAGCCGCGGCGCCGAGA
AGCTCTGGCGACTGGTCAATGGCGAGGCCCGCAAGGGCTACGTCAACTGCCTCGGCGCGCTGACCGGCGGCCAGGCCATG
CAGCAGGTCAAGGCAGGTATCGAGGCGATCTATCTCTCGGGCTGGCAGGTCGCCGCCGACAACAACAGCTACCTGTCGAT
GTATCCCGACCAGTCGCTCTATCCGGTGGACTCGGTGCCCAAGGTCGTCGAACGCATCAACAACAGTTTCCGCCGCGCGG
ATCAGATCCAGTGGCAGAAGGGCGCCAACCCCGGCGATGCCGACTTCGTCGATTACTTCGCCCCCATCGTCGCCGACGCC
GAGGCGGGATTCGGCGGCGTGCTCAACGCCTATGAACTGATGACGGCAATGATCCGTGCCGGTGCCAGTGGCGTGCATTT
CGAGGATCAGCTCGCCGCGGTCAAGAAGTGCGGCCACATGGGCGGCAAGGTGCTGGTGCCCACGCAGGAGGCCGTGCAGA
AGCTGGTCGCCGCCCGTCTGGCCGCCGACGTCGCGGGCACGCCGACGCTGGTCATCGCGCGCACCGACGCCAATGCCGCC
AACCTGATCACCGCCGACGTGGATGATTACGACAAGCCCTTCATCACCGGGGAACGCACCGCCGAAGGCTTCTATCGGGT
CAATGCCGGCCTCGATCAGGCCATCTCGCGAGGCCTGGCCTACGCGCCCTTCGCCGACATCATCTGGTGCGAAACCGCCA
AGCCGGATCTCGACGAAGCCAGGCGCTTCGCCGAGGCGATCCATCGCGAATATCCGGGGCAACTGCTCGCCTACAACTGC
TCGCCGTCCTTCAACTGGAAGAAGAACCTCGACGATGCCGAGATCGCCGGGTTCCAGCAGGCCCTGGCCGACATGGGCTA
CACCTACCAGTTCATCACCTTAGCGGGCATTCACAACATGTGGTACAACATGTTCGATCTCGCCCACAGCTACGCTCAGG
GCGAAGGCATGAAGCACTACGTCGAGAAGGTCCAGCAGCCGGAATTCGAGGCCGCCGAACGCGGCTACACCTTCGTCGCT
CACCAGCAGGAAGTGGGCACCGGCTACTTCGACGACATGACCAACGTCATCCAGGGCGGAGTGTCGTCGGTGACCGCCCT
CAAGGGCTCCACCGAGGAAGCGCAGTTCTGA

Upstream 100 bases:

>100_bases
CACCAGGCCCGCCCTGGCGAAAAAGACGGCACCTCGGTTCGACGTGGATCGCCCTTTCGGGCTCGCCCCACATGCTCACG
ACGACCAAGAGGCAAGCGAC

Downstream 100 bases:

>100_bases
CACGCTGCTCGCTCCCCCAACGAAAAGGGCGCCCGATGGGGCGCCCTTTTCATTGCCTGACCGGCGATCCGATCAACGCT
GCTCGCGAAGGATGCCCAGC

Product: isocitrate lyase

Products: NA

Alternate protein names: ICL; Isocitrase; Isocitratase [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSQTREQQIAALEKDWNENPRWKDVKRPYSAEDVVRLRGSVNEAHTLASRGAEKLWRLVNGEARKGYVNCLGALTGGQAM
QQVKAGIEAIYLSGWQVAADNNSYLSMYPDQSLYPVDSVPKVVERINNSFRRADQIQWQKGANPGDADFVDYFAPIVADA
EAGFGGVLNAYELMTAMIRAGASGVHFEDQLAAVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTPTLVIARTDANAA
NLITADVDDYDKPFITGERTAEGFYRVNAGLDQAISRGLAYAPFADIIWCETAKPDLDEARRFAEAIHREYPGQLLAYNC
SPSFNWKKNLDDAEIAGFQQALADMGYTYQFITLAGIHNMWYNMFDLAHSYAQGEGMKHYVEKVQQPEFEAAERGYTFVA
HQQEVGTGYFDDMTNVIQGGVSSVTALKGSTEEAQF

Sequences:

>Translated_436_residues
MSQTREQQIAALEKDWNENPRWKDVKRPYSAEDVVRLRGSVNEAHTLASRGAEKLWRLVNGEARKGYVNCLGALTGGQAM
QQVKAGIEAIYLSGWQVAADNNSYLSMYPDQSLYPVDSVPKVVERINNSFRRADQIQWQKGANPGDADFVDYFAPIVADA
EAGFGGVLNAYELMTAMIRAGASGVHFEDQLAAVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTPTLVIARTDANAA
NLITADVDDYDKPFITGERTAEGFYRVNAGLDQAISRGLAYAPFADIIWCETAKPDLDEARRFAEAIHREYPGQLLAYNC
SPSFNWKKNLDDAEIAGFQQALADMGYTYQFITLAGIHNMWYNMFDLAHSYAQGEGMKHYVEKVQQPEFEAAERGYTFVA
HQQEVGTGYFDDMTNVIQGGVSSVTALKGSTEEAQF
>Mature_435_residues
SQTREQQIAALEKDWNENPRWKDVKRPYSAEDVVRLRGSVNEAHTLASRGAEKLWRLVNGEARKGYVNCLGALTGGQAMQ
QVKAGIEAIYLSGWQVAADNNSYLSMYPDQSLYPVDSVPKVVERINNSFRRADQIQWQKGANPGDADFVDYFAPIVADAE
AGFGGVLNAYELMTAMIRAGASGVHFEDQLAAVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTPTLVIARTDANAAN
LITADVDDYDKPFITGERTAEGFYRVNAGLDQAISRGLAYAPFADIIWCETAKPDLDEARRFAEAIHREYPGQLLAYNCS
PSFNWKKNLDDAEIAGFQQALADMGYTYQFITLAGIHNMWYNMFDLAHSYAQGEGMKHYVEKVQQPEFEAAERGYTFVAH
QQEVGTGYFDDMTNVIQGGVSSVTALKGSTEEAQF

Specific function: Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle. May be involved in the assimilation of one-carbon compounds via the isocitrate lyase- positive serine pathway [H]

COG id: COG2224

COG function: function code C; Isocitrate lyase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1790445, Length=434, Percent_Identity=76.036866359447, Blast_Score=713, Evalue=0.0,
Organism=Escherichia coli, GI1786525, Length=260, Percent_Identity=28.0769230769231, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71982926, Length=423, Percent_Identity=55.5555555555556, Blast_Score=475, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI17561814, Length=423, Percent_Identity=55.5555555555556, Blast_Score=475, Evalue=1e-134,
Organism=Saccharomyces cerevisiae, GI6320908, Length=296, Percent_Identity=36.4864864864865, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6325263, Length=249, Percent_Identity=36.5461847389558, Blast_Score=156, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006254
- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.1 [H]

Molecular weight: Translated: 48034; Mature: 47903

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTREQQIAALEKDWNENPRWKDVKRPYSAEDVVRLRGSVNEAHTLASRGAEKLWRLVN
CCCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GEARKGYVNCLGALTGGQAMQQVKAGIEAIYLSGWQVAADNNSYLSMYPDQSLYPVDSVP
CCCCCCHHHHHHHHHCHHHHHHHHHCHHEEEECCEEEEECCCCEEEECCCCCCCCCHHHH
KVVERINNSFRRADQIQWQKGANPGDADFVDYFAPIVADAEAGFGGVLNAYELMTAMIRA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GASGVHFEDQLAAVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTPTLVIARTDANAA
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
NLITADVDDYDKPFITGERTAEGFYRVNAGLDQAISRGLAYAPFADIIWCETAKPDLDEA
CEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCHHEEEECCCCCCHHHH
RRFAEAIHREYPGQLLAYNCSPSFNWKKNLDDAEIAGFQQALADMGYTYQFITLAGIHNM
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
WYNMFDLAHSYAQGEGMKHYVEKVQQPEFEAAERGYTFVAHQQEVGTGYFDDMTNVIQGG
HHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCEEEEEHHHHCCCHHHHHHHHHHCC
VSSVTALKGSTEEAQF
HHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SQTREQQIAALEKDWNENPRWKDVKRPYSAEDVVRLRGSVNEAHTLASRGAEKLWRLVN
CCHHHHHHHHHHHCCCCCCCCCHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
GEARKGYVNCLGALTGGQAMQQVKAGIEAIYLSGWQVAADNNSYLSMYPDQSLYPVDSVP
CCCCCCHHHHHHHHHCHHHHHHHHHCHHEEEECCEEEEECCCCEEEECCCCCCCCCHHHH
KVVERINNSFRRADQIQWQKGANPGDADFVDYFAPIVADAEAGFGGVLNAYELMTAMIRA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GASGVHFEDQLAAVKKCGHMGGKVLVPTQEAVQKLVAARLAADVAGTPTLVIARTDANAA
CCCCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
NLITADVDDYDKPFITGERTAEGFYRVNAGLDQAISRGLAYAPFADIIWCETAKPDLDEA
CEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCCCCCCHHEEEECCCCCCHHHH
RRFAEAIHREYPGQLLAYNCSPSFNWKKNLDDAEIAGFQQALADMGYTYQFITLAGIHNM
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
WYNMFDLAHSYAQGEGMKHYVEKVQQPEFEAAERGYTFVAHQQEVGTGYFDDMTNVIQGG
HHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHCCCEEEEEHHHHCCCHHHHHHHHHHCC
VSSVTALKGSTEEAQF
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]