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The map label for this gene is dusB [H]

Identifier: 92114408

GI number: 92114408

Start: 2575837

End: 2576868

Strand: Direct

Name: dusB [H]

Synonym: Csal_2287

Alternate gene names: 92114408

Gene position: 2575837-2576868 (Clockwise)

Preceding gene: 92114407

Following gene: 92114409

Centisome position: 69.68

GC content: 66.57

Gene sequence:

>1032_bases
ATGCCGACCCACGTCTCATCTCAGGCGATGCCGTTGCCGCGAATCGGCGCCTACGCATTGTCCAGCCGAGCGATTCTCGC
GCCCATGGCCGGCGTGACGGATCGCCCGTTTCGTCAGTTGTGCCGGGAGCTGGGCGCGGGGCTGGTGGTATCCGAAATGG
TGACGTCCGACACGCGCCTGTGGCATACCCGCAAATCGCGCCAGCGCCTCGACCACACCGGCGAGCCCGGCCCGCGTGCC
GTGCAGATCGCAGGCGGCGATGCCGCGATGCTGGCCGAGGCCGCGCGCCTCAACGTTGCCCAGGGCGCCGAGATCGTCGA
CATCAACATGGGCTGCCCGGCCAAGAAGGTATGCAACAAGGCCGCCGGCTCGGCATTGTTGCGCGACGAACGCCTGGTCG
CGGAGATCCTCGAGGCGGTCGTCGCCGCCGTGGATGTCCCGGTGACCCTGAAGATTCGAACCGGCTGGTGTCCGCAAACC
CGCAATGGCGTACGGGTCGCCAAGCTTGCCGAGTCGGCGGGCATCCAGGCCCTTGCCGTGCATGGGCGCACGCGTGAGCA
GCGCTATCGCGGCGAGGCCGAATACGACACCATCGCCGCCATCAAGCAGGCGGTCTCGCTGCCGGTCTTCGCCAACGGCG
ACATCGACGGCGCCGAGAAAGCTGCCCGCGTCCTCGACTACACTAAGGCGGATGCAGTGATGATCGGCCGCGGCGCCCAG
GGCAATCCCTGGATCTTCCGCGAGATCGATCACTACCTGCGTACCGGCGACTGCCTGCCGCGCCCGACGCCCGACGATAT
CGCCACCCTGATGCACCGTCATCTCGAGGCATTGCATGCCTTCTACGGTGAGCACATGGGCGTGCGCATCGCGCGCAAGC
ATGTCGGCTGGTATCTGGCGACGCAACCGCAAGCCGCGGCACTACGCGCACGCTTCAACGTACTGGAACAGCCCTCGGCC
CAACACCGTTTCGTGGATGCCTTGGCCCACGACACGCTGGAACTGGCCTCAACTGGAAGCAATGCAGCATGA

Upstream 100 bases:

>100_bases
TGAGCGCGCCTGGCGCGAGGGATGGCAGCCGGCCCCCCTTCAAGAACGTCGCCGCGGCGGCTATCATAGTCGCCTTTCAG
CCATCTAGACACGTCCCGTG

Downstream 100 bases:

>100_bases
CCAGCAGGCAAGCATTCCCCATCGATCAGAATACGCTCGATGCCGGCGCCACCACCTCGCCAGCGCATCAGCAAACCCTG
CGTGAGTCGGTCGAGCTCGC

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MPTHVSSQAMPLPRIGAYALSSRAILAPMAGVTDRPFRQLCRELGAGLVVSEMVTSDTRLWHTRKSRQRLDHTGEPGPRA
VQIAGGDAAMLAEAARLNVAQGAEIVDINMGCPAKKVCNKAAGSALLRDERLVAEILEAVVAAVDVPVTLKIRTGWCPQT
RNGVRVAKLAESAGIQALAVHGRTREQRYRGEAEYDTIAAIKQAVSLPVFANGDIDGAEKAARVLDYTKADAVMIGRGAQ
GNPWIFREIDHYLRTGDCLPRPTPDDIATLMHRHLEALHAFYGEHMGVRIARKHVGWYLATQPQAAALRARFNVLEQPSA
QHRFVDALAHDTLELASTGSNAA

Sequences:

>Translated_343_residues
MPTHVSSQAMPLPRIGAYALSSRAILAPMAGVTDRPFRQLCRELGAGLVVSEMVTSDTRLWHTRKSRQRLDHTGEPGPRA
VQIAGGDAAMLAEAARLNVAQGAEIVDINMGCPAKKVCNKAAGSALLRDERLVAEILEAVVAAVDVPVTLKIRTGWCPQT
RNGVRVAKLAESAGIQALAVHGRTREQRYRGEAEYDTIAAIKQAVSLPVFANGDIDGAEKAARVLDYTKADAVMIGRGAQ
GNPWIFREIDHYLRTGDCLPRPTPDDIATLMHRHLEALHAFYGEHMGVRIARKHVGWYLATQPQAAALRARFNVLEQPSA
QHRFVDALAHDTLELASTGSNAA
>Mature_342_residues
PTHVSSQAMPLPRIGAYALSSRAILAPMAGVTDRPFRQLCRELGAGLVVSEMVTSDTRLWHTRKSRQRLDHTGEPGPRAV
QIAGGDAAMLAEAARLNVAQGAEIVDINMGCPAKKVCNKAAGSALLRDERLVAEILEAVVAAVDVPVTLKIRTGWCPQTR
NGVRVAKLAESAGIQALAVHGRTREQRYRGEAEYDTIAAIKQAVSLPVFANGDIDGAEKAARVLDYTKADAVMIGRGAQG
NPWIFREIDHYLRTGDCLPRPTPDDIATLMHRHLEALHAFYGEHMGVRIARKHVGWYLATQPQAAALRARFNVLEQPSAQ
HRFVDALAHDTLELASTGSNAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.4728033472803, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI239788462, Length=241, Percent_Identity=33.195020746888, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI31742496, Length=225, Percent_Identity=32.4444444444444, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI40807366, Length=246, Percent_Identity=32.1138211382114, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI8923374, Length=245, Percent_Identity=32.6530612244898, Blast_Score=102, Evalue=5e-22,
Organism=Escherichia coli, GI1789660, Length=314, Percent_Identity=58.5987261146497, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1788462, Length=259, Percent_Identity=34.3629343629344, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693211, Length=243, Percent_Identity=30.8641975308642, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=250, Percent_Identity=32.4, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=226, Percent_Identity=31.858407079646, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17510279, Length=255, Percent_Identity=31.7647058823529, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17507177, Length=236, Percent_Identity=30.5084745762712, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323433, Length=267, Percent_Identity=29.5880149812734, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323560, Length=233, Percent_Identity=33.0472103004292, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.2035398230088, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24580595, Length=230, Percent_Identity=35.2173913043478, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19920448, Length=230, Percent_Identity=35.2173913043478, Blast_Score=128, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24585320, Length=250, Percent_Identity=32.8, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI45549423, Length=264, Percent_Identity=29.9242424242424, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI19921524, Length=228, Percent_Identity=28.5087719298246, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37135; Mature: 37004

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTHVSSQAMPLPRIGAYALSSRAILAPMAGVTDRPFRQLCRELGAGLVVSEMVTSDTRL
CCCCCCCCCCCCCCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHH
WHTRKSRQRLDHTGEPGPRAVQIAGGDAAMLAEAARLNVAQGAEIVDINMGCPAKKVCNK
HHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
AAGSALLRDERLVAEILEAVVAAVDVPVTLKIRTGWCPQTRNGVRVAKLAESAGIQALAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEE
HGRTREQRYRGEAEYDTIAAIKQAVSLPVFANGDIDGAEKAARVLDYTKADAVMIGRGAQ
CCCCHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCC
GNPWIFREIDHYLRTGDCLPRPTPDDIATLMHRHLEALHAFYGEHMGVRIARKHVGWYLA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEE
TQPQAAALRARFNVLEQPSAQHRFVDALAHDTLELASTGSNAA
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PTHVSSQAMPLPRIGAYALSSRAILAPMAGVTDRPFRQLCRELGAGLVVSEMVTSDTRL
CCCCCCCCCCCCCCCHHHHCCCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCHHH
WHTRKSRQRLDHTGEPGPRAVQIAGGDAAMLAEAARLNVAQGAEIVDINMGCPAKKVCNK
HHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHH
AAGSALLRDERLVAEILEAVVAAVDVPVTLKIRTGWCPQTRNGVRVAKLAESAGIQALAV
HHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCEEEEE
HGRTREQRYRGEAEYDTIAAIKQAVSLPVFANGDIDGAEKAARVLDYTKADAVMIGRGAQ
CCCCHHHHHCCCCHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHCCCCEEEEECCCC
GNPWIFREIDHYLRTGDCLPRPTPDDIATLMHRHLEALHAFYGEHMGVRIARKHVGWYLA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCEEEE
TQPQAAALRARFNVLEQPSAQHRFVDALAHDTLELASTGSNAA
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]