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The map label for this gene is gatB [H]

Identifier: 92114365

GI number: 92114365

Start: 2516533

End: 2517987

Strand: Direct

Name: gatB [H]

Synonym: Csal_2243

Alternate gene names: 92114365

Gene position: 2516533-2517987 (Clockwise)

Preceding gene: 92114364

Following gene: 92114376

Centisome position: 68.08

GC content: 63.71

Gene sequence:

>1455_bases
ATGATGCAGTGGGAAACCGTGATCGGGCTCGAGGTCCACGTTCAGCTCGCCACCCAGTCCAAGATCTTCTCCGGAGCGTC
CACCGCCTTCGGCGCCGAGCCCAACACCCAGGCGTGCGCTGTCGACCTCGGCATGCCCGGCGTGCTGCCGGTGCTCAATG
AAAACGCCGTGGCGATGGCGGTGAAATTCGGTCTCGCCGTCGACGCCGAGATCCCCGAGACCTCGCTGTTCGAGCGCAAG
AACTACTTCTACCCGGATTTGCCCAAGGGATATCAGACGAGCCAGATGGCGCAGCCCATCGTCGGCCCCGGCAAGGTCGA
GATCACCCTCGACGACGGTTCGACGCGCAGCGTCCGCATTCATCACGCTCATCTCGAGGAAGATGCCGGCAAGTCGCTGC
ATGAGGACTATCACGGCATGACCGGCATCGATCTCAATCGTGCCGGCACGCCGCTGCTGGAGATCGTCTCCGAGCCCGAC
ATGCGCTCGGCCAAGGAAGCGGTCGCCTACATCAAGGCGCTGCACGCCATCGTCACCTATCTCGGTATCTCCGACGGCAA
CATGGCCGAAGGCTCGATGCGCTGCGACGTCAACGTCTCGGTACGTCCCAAGGGGCAGGCCGCTTTCGGCACCCGCGCCG
AGATCAAGAACGTCAACTCGTTTCGCTTCGTCGAGAAAGCCATCGCCTTCGAGGTCGAGCGCCAGATCGAACTCATCGAA
GATGGCGGTCAGGTCGTGCAGGAAACTCGACTGTTCGACCCGGAACGCGACGAGACGCGCGGCATGCGCACCAAGGAAGA
AGCCAACGACTACCGTTACTTCCCCTGCCCCGACCTGCTTCCGGTGACGCTCGACGGTGCCTATGTCGATCACCAGCGCG
CGCTCATGCCCGAACTGCCCAGCGAAAAGCGCCACCGCTTCGAAAACCAGCTGGGCTTGTCCGCCTATGACGCCGGCGTG
CTGGCCTCGAGCCGTGCCATGGCCGAGTACTTCGAAGCCGTCAAGGATGTCTGCGGCGACGCCAAGCAGGCCGCCAACTG
GGTGCAAGGCGAACTCTCGGCCAACCTCAACCGCGAAAACCTGGAGATCGGCGATAGTCCGGTCACGGCCGAGCAACTCG
GCGGCCTCATCCAGCGCGTCATGGACGACACCATCAGCGGCAAGGCCGCCAAGCAGGTCTTCCAGGCGCTGTGGGACGGC
GAGGCGGACAGTGCCGATGCCATCATCGAGGCCCGCGGACTCAAGCAGGTCACCGACACCGGCGCCATCGAAGGCATGAT
CGACCAGGTCATCGCCGACAGCCCGGTTCAGGTCGCCCAGTACCGCGAAGCCGACGAGGCCAAGCGCGGCAAGATGATCG
GCTACTTCGTCGGCCAGGTCATGAAAGCCTCGCGCGGCACCGCCAACCCGCAACAGGTCAACAGCCTCCTCAAGCAAAAG
CTGGATCAGGCTTGA

Upstream 100 bases:

>100_bases
CTCCGCACTTCGCCGAGGCCCAGCTGCTCGGCGTGGCGCATCAATTCCAGCAGGCCACCGACTGGCACCTCAAGCGTCCG
GCATTCGGCAAGGAGATGGC

Downstream 100 bases:

>100_bases
GCCAACGCTGACTCGCCGGGTCAGCACCAGCGAAAAAGCCCATGGCCAGTGCCATGGGCTTTTTATTGCGAAACCTTATC
GGGCTCGGTATCAATTGGTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MMQWETVIGLEVHVQLATQSKIFSGASTAFGAEPNTQACAVDLGMPGVLPVLNENAVAMAVKFGLAVDAEIPETSLFERK
NYFYPDLPKGYQTSQMAQPIVGPGKVEITLDDGSTRSVRIHHAHLEEDAGKSLHEDYHGMTGIDLNRAGTPLLEIVSEPD
MRSAKEAVAYIKALHAIVTYLGISDGNMAEGSMRCDVNVSVRPKGQAAFGTRAEIKNVNSFRFVEKAIAFEVERQIELIE
DGGQVVQETRLFDPERDETRGMRTKEEANDYRYFPCPDLLPVTLDGAYVDHQRALMPELPSEKRHRFENQLGLSAYDAGV
LASSRAMAEYFEAVKDVCGDAKQAANWVQGELSANLNRENLEIGDSPVTAEQLGGLIQRVMDDTISGKAAKQVFQALWDG
EADSADAIIEARGLKQVTDTGAIEGMIDQVIADSPVQVAQYREADEAKRGKMIGYFVGQVMKASRGTANPQQVNSLLKQK
LDQA

Sequences:

>Translated_484_residues
MMQWETVIGLEVHVQLATQSKIFSGASTAFGAEPNTQACAVDLGMPGVLPVLNENAVAMAVKFGLAVDAEIPETSLFERK
NYFYPDLPKGYQTSQMAQPIVGPGKVEITLDDGSTRSVRIHHAHLEEDAGKSLHEDYHGMTGIDLNRAGTPLLEIVSEPD
MRSAKEAVAYIKALHAIVTYLGISDGNMAEGSMRCDVNVSVRPKGQAAFGTRAEIKNVNSFRFVEKAIAFEVERQIELIE
DGGQVVQETRLFDPERDETRGMRTKEEANDYRYFPCPDLLPVTLDGAYVDHQRALMPELPSEKRHRFENQLGLSAYDAGV
LASSRAMAEYFEAVKDVCGDAKQAANWVQGELSANLNRENLEIGDSPVTAEQLGGLIQRVMDDTISGKAAKQVFQALWDG
EADSADAIIEARGLKQVTDTGAIEGMIDQVIADSPVQVAQYREADEAKRGKMIGYFVGQVMKASRGTANPQQVNSLLKQK
LDQA
>Mature_484_residues
MMQWETVIGLEVHVQLATQSKIFSGASTAFGAEPNTQACAVDLGMPGVLPVLNENAVAMAVKFGLAVDAEIPETSLFERK
NYFYPDLPKGYQTSQMAQPIVGPGKVEITLDDGSTRSVRIHHAHLEEDAGKSLHEDYHGMTGIDLNRAGTPLLEIVSEPD
MRSAKEAVAYIKALHAIVTYLGISDGNMAEGSMRCDVNVSVRPKGQAAFGTRAEIKNVNSFRFVEKAIAFEVERQIELIE
DGGQVVQETRLFDPERDETRGMRTKEEANDYRYFPCPDLLPVTLDGAYVDHQRALMPELPSEKRHRFENQLGLSAYDAGV
LASSRAMAEYFEAVKDVCGDAKQAANWVQGELSANLNRENLEIGDSPVTAEQLGGLIQRVMDDTISGKAAKQVFQALWDG
EADSADAIIEARGLKQVTDTGAIEGMIDQVIADSPVQVAQYREADEAKRGKMIGYFVGQVMKASRGTANPQQVNSLLKQK
LDQA

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=500, Percent_Identity=38, Blast_Score=342, Evalue=6e-94,
Organism=Caenorhabditis elegans, GI115534094, Length=311, Percent_Identity=38.2636655948553, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=525, Percent_Identity=31.047619047619, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550808, Length=502, Percent_Identity=34.0637450199203, Blast_Score=300, Evalue=2e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 52912; Mature: 52912

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQWETVIGLEVHVQLATQSKIFSGASTAFGAEPNTQACAVDLGMPGVLPVLNENAVAMA
CCCCEEEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEE
VKFGLAVDAEIPETSLFERKNYFYPDLPKGYQTSQMAQPIVGPGKVEITLDDGSTRSVRI
EEECEEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCEEEE
HHAHLEEDAGKSLHEDYHGMTGIDLNRAGTPLLEIVSEPDMRSAKEAVAYIKALHAIVTY
EEECCCHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LGISDGNMAEGSMRCDVNVSVRPKGQAAFGTRAEIKNVNSFRFVEKAIAFEVERQIELIE
HCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DGGQVVQETRLFDPERDETRGMRTKEEANDYRYFPCPDLLPVTLDGAYVDHQRALMPELP
CCHHHHHHHHCCCCCCHHHCCCCCHHHCCCCEECCCCCCCEEEECCCEECCHHHHCCCCC
SEKRHRFENQLGLSAYDAGVLASSRAMAEYFEAVKDVCGDAKQAANWVQGELSANLNREN
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
LEIGDSPVTAEQLGGLIQRVMDDTISGKAAKQVFQALWDGEADSADAIIEARGLKQVTDT
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHH
GAIEGMIDQVIADSPVQVAQYREADEAKRGKMIGYFVGQVMKASRGTANPQQVNSLLKQK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LDQA
HCCC
>Mature Secondary Structure
MMQWETVIGLEVHVQLATQSKIFSGASTAFGAEPNTQACAVDLGMPGVLPVLNENAVAMA
CCCCEEEEEEEEEEEEECHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEE
VKFGLAVDAEIPETSLFERKNYFYPDLPKGYQTSQMAQPIVGPGKVEITLDDGSTRSVRI
EEECEEEECCCCCHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCCCEEEE
HHAHLEEDAGKSLHEDYHGMTGIDLNRAGTPLLEIVSEPDMRSAKEAVAYIKALHAIVTY
EEECCCHHHCHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
LGISDGNMAEGSMRCDVNVSVRPKGQAAFGTRAEIKNVNSFRFVEKAIAFEVERQIELIE
HCCCCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DGGQVVQETRLFDPERDETRGMRTKEEANDYRYFPCPDLLPVTLDGAYVDHQRALMPELP
CCHHHHHHHHCCCCCCHHHCCCCCHHHCCCCEECCCCCCCEEEECCCEECCHHHHCCCCC
SEKRHRFENQLGLSAYDAGVLASSRAMAEYFEAVKDVCGDAKQAANWVQGELSANLNREN
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCC
LEIGDSPVTAEQLGGLIQRVMDDTISGKAAKQVFQALWDGEADSADAIIEARGLKQVTDT
CCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCHHHHHH
GAIEGMIDQVIADSPVQVAQYREADEAKRGKMIGYFVGQVMKASRGTANPQQVNSLLKQK
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
LDQA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA