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The map label for this gene is gatA

Identifier: 92114364

GI number: 92114364

Start: 2515076

End: 2516536

Strand: Direct

Name: gatA

Synonym: Csal_2242

Alternate gene names: 92114364

Gene position: 2515076-2516536 (Clockwise)

Preceding gene: 92114363

Following gene: 92114365

Centisome position: 68.04

GC content: 66.94

Gene sequence:

>1461_bases
ATGCATGACAAGACCCTTACGGAGCTTGCCGCCGGCCTGCGCGCGGGCGAGTTCTCGAGCCGTGAGCTCTGCCAGTCGGC
GCTCGATCGCATCCAGCGCCTCGACGGCGACCTCAACAGCTTCATCAGCGTGACCGCCGAACAGGCCCTGGCCGACGCCG
ACGCCGCCGACCAGGCTCGCGCATCGGGCGATGCCGCCGCCCTTGCCGGCCTGCCCCTGGCGCTCAAGGACATCTTCTGC
ACCCAGGGCGTACGCACCAGTTGCGGCTCGAAGATGCTCGACAGCTTCGTCGCGCCCTATGACGCCACGGTCGTCGAGAA
GATCCGCCAGGCGGGTGGTGTCAGCCTCGGCAAGACCAACATGGACGAGTTCGCCATGGGCTCGTCGAACGAAAACAGCT
TCTATGGCCCGGTCAAGAACCCCTGGGACTTGAGCGCCGTGCCCGGCGGCTCCTCGGGCGGTAGCGCCGCCGCCGTGGCG
GCGGGCCTGGTACCGGCAGCGCTCGGCACCGATACCGGCGGTTCGATTCGTCAGCCGGCCGCCTTTTGCGGCATAACCGG
CCTGAAACCCACCTACGGGCGCGTGTCGCGCTACGGCATGGTGGCCTACGCATCGAGCCTCGACCAGGGCGGCCCCATGG
CACGCACCGCCGCCGACTGTGCCCTGCTGCTCGAAACCATCGCCGGCCACGACGTGCGCGATTCCACCAGCGTGGCACGC
GTGGTGCCCGACTACTCCGCCGAGCTCGACACCTCGCTGGCCGGCCTCAAGATCGGGCTGCCCAAGGAATATTTCGGCGA
CGGACTCGACCCCGAGGTCGAAACCGCCGTGCGTGAAGCGATCAAGGTCTACGAGTCGCTCGGTGCCAAGGTCTGCGAAG
TCAGCCTGCCGCACACGCACCTGGCGATTCCTGCCTACTACGTCATTGCACCGGCGGAGGCATCGTCCAACCTGTCGCGT
TATGACGGCGTGCGCTTCGGCCACCGCTGCGAGGATCCGACCGACCTCGAGGACCTCTACAAGCGCACGCGGGCCGAAGG
CTTCGGCGAAGAGGTCAAGCGGCGCATCCTGATCGGCACGCACACGCTTTCCGAAGGGTTCTTCGACGCTTACTACCTCA
AGGCACAGCAAGTGCGCCGCCTGATCCGCCAGGACTTTCTCGACGCCTTCGACGAAGTCGACGTGCTCATGGGGCCGACC
GCCCCCACGCCGGCCTTCGACCTCGGCGCGCAGAAAGATCCGGTCTCGATGTACCTGCAGGATATCTACACCATCGCCGT
CAACCTGGCCGGCATTCCGGGCATCAGCGTTCCGGCCGGCTTTGCCGGTAACCGCCCCGTAGGCCTCCAGGTGCTGGCTC
CGCACTTCGCCGAGGCCCAGCTGCTCGGCGTGGCGCATCAATTCCAGCAGGCCACCGACTGGCACCTCAAGCGTCCGGCA
TTCGGCAAGGAGATGGCATGA

Upstream 100 bases:

>100_bases
CCAGGCCTGCGCCCCCGTCACCGAGGGCGGTCTCTACCTCGTCCCGCGCGTCGTCGAATGAGCCGTCTGTCAGGACCCGA
CACAGGAGTCACCTTCTTCC

Downstream 100 bases:

>100_bases
TGCAGTGGGAAACCGTGATCGGGCTCGAGGTCCACGTTCAGCTCGCCACCCAGTCCAAGATCTTCTCCGGAGCGTCCACC
GCCTTCGGCGCCGAGCCCAA

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MHDKTLTELAAGLRAGEFSSRELCQSALDRIQRLDGDLNSFISVTAEQALADADAADQARASGDAAALAGLPLALKDIFC
TQGVRTSCGSKMLDSFVAPYDATVVEKIRQAGGVSLGKTNMDEFAMGSSNENSFYGPVKNPWDLSAVPGGSSGGSAAAVA
AGLVPAALGTDTGGSIRQPAAFCGITGLKPTYGRVSRYGMVAYASSLDQGGPMARTAADCALLLETIAGHDVRDSTSVAR
VVPDYSAELDTSLAGLKIGLPKEYFGDGLDPEVETAVREAIKVYESLGAKVCEVSLPHTHLAIPAYYVIAPAEASSNLSR
YDGVRFGHRCEDPTDLEDLYKRTRAEGFGEEVKRRILIGTHTLSEGFFDAYYLKAQQVRRLIRQDFLDAFDEVDVLMGPT
APTPAFDLGAQKDPVSMYLQDIYTIAVNLAGIPGISVPAGFAGNRPVGLQVLAPHFAEAQLLGVAHQFQQATDWHLKRPA
FGKEMA

Sequences:

>Translated_486_residues
MHDKTLTELAAGLRAGEFSSRELCQSALDRIQRLDGDLNSFISVTAEQALADADAADQARASGDAAALAGLPLALKDIFC
TQGVRTSCGSKMLDSFVAPYDATVVEKIRQAGGVSLGKTNMDEFAMGSSNENSFYGPVKNPWDLSAVPGGSSGGSAAAVA
AGLVPAALGTDTGGSIRQPAAFCGITGLKPTYGRVSRYGMVAYASSLDQGGPMARTAADCALLLETIAGHDVRDSTSVAR
VVPDYSAELDTSLAGLKIGLPKEYFGDGLDPEVETAVREAIKVYESLGAKVCEVSLPHTHLAIPAYYVIAPAEASSNLSR
YDGVRFGHRCEDPTDLEDLYKRTRAEGFGEEVKRRILIGTHTLSEGFFDAYYLKAQQVRRLIRQDFLDAFDEVDVLMGPT
APTPAFDLGAQKDPVSMYLQDIYTIAVNLAGIPGISVPAGFAGNRPVGLQVLAPHFAEAQLLGVAHQFQQATDWHLKRPA
FGKEMA
>Mature_486_residues
MHDKTLTELAAGLRAGEFSSRELCQSALDRIQRLDGDLNSFISVTAEQALADADAADQARASGDAAALAGLPLALKDIFC
TQGVRTSCGSKMLDSFVAPYDATVVEKIRQAGGVSLGKTNMDEFAMGSSNENSFYGPVKNPWDLSAVPGGSSGGSAAAVA
AGLVPAALGTDTGGSIRQPAAFCGITGLKPTYGRVSRYGMVAYASSLDQGGPMARTAADCALLLETIAGHDVRDSTSVAR
VVPDYSAELDTSLAGLKIGLPKEYFGDGLDPEVETAVREAIKVYESLGAKVCEVSLPHTHLAIPAYYVIAPAEASSNLSR
YDGVRFGHRCEDPTDLEDLYKRTRAEGFGEEVKRRILIGTHTLSEGFFDAYYLKAQQVRRLIRQDFLDAFDEVDVLMGPT
APTPAFDLGAQKDPVSMYLQDIYTIAVNLAGIPGISVPAGFAGNRPVGLQVLAPHFAEAQLLGVAHQFQQATDWHLKRPA
FGKEMA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=503, Percent_Identity=39.7614314115308, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI195972892, Length=492, Percent_Identity=28.8617886178862, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI166795287, Length=256, Percent_Identity=32.421875, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17543272, Length=440, Percent_Identity=40, Blast_Score=291, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=508, Percent_Identity=25.3937007874016, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=28.6184210526316, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71990152, Length=304, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=315, Percent_Identity=28.8888888888889, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556278, Length=315, Percent_Identity=28.8888888888889, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538252, Length=249, Percent_Identity=28.5140562248996, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=324, Percent_Identity=25.6172839506173, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=468, Percent_Identity=34.1880341880342, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=460, Percent_Identity=30.2173913043478, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=471, Percent_Identity=25.9023354564756, Blast_Score=81, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648113, Length=486, Percent_Identity=42.1810699588477, Blast_Score=333, Evalue=1e-91,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=28.3168316831683, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24652985, Length=495, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI19922090, Length=495, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652981, Length=495, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24652983, Length=495, Percent_Identity=27.2727272727273, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24648435, Length=482, Percent_Identity=24.896265560166, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24648437, Length=482, Percent_Identity=24.896265560166, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI45550774, Length=482, Percent_Identity=24.896265560166, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078093, Length=498, Percent_Identity=25.9036144578313, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356731, Length=501, Percent_Identity=24.9500998003992, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24648441, Length=417, Percent_Identity=25.4196642685851, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24648439, Length=417, Percent_Identity=25.4196642685851, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_CHRSD (Q1QVB5)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574292.1
- ProteinModelPortal:   Q1QVB5
- SMR:   Q1QVB5
- STRING:   Q1QVB5
- GeneID:   4026052
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2242
- NMPDR:   fig|290398.4.peg.1412
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- BioCyc:   CSAL290398:CSAL_2242-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51550; Mature: 51550

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 76-76 ACT_SITE 151-151 ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDKTLTELAAGLRAGEFSSRELCQSALDRIQRLDGDLNSFISVTAEQALADADAADQAR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
ASGDAAALAGLPLALKDIFCTQGVRTSCGSKMLDSFVAPYDATVVEKIRQAGGVSLGKTN
CCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
MDEFAMGSSNENSFYGPVKNPWDLSAVPGGSSGGSAAAVAAGLVPAALGTDTGGSIRQPA
HHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
AFCGITGLKPTYGRVSRYGMVAYASSLDQGGPMARTAADCALLLETIAGHDVRDSTSVAR
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
VVPDYSAELDTSLAGLKIGLPKEYFGDGLDPEVETAVREAIKVYESLGAKVCEVSLPHTH
HCCCCHHHHCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCE
LAIPAYYVIAPAEASSNLSRYDGVRFGHRCEDPTDLEDLYKRTRAEGFGEEVKRRILIGT
EECCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHEEE
HTLSEGFFDAYYLKAQQVRRLIRQDFLDAFDEVDVLMGPTAPTPAFDLGAQKDPVSMYLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
DIYTIAVNLAGIPGISVPAGFAGNRPVGLQVLAPHFAEAQLLGVAHQFQQATDWHLKRPA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
FGKEMA
CCCCCC
>Mature Secondary Structure
MHDKTLTELAAGLRAGEFSSRELCQSALDRIQRLDGDLNSFISVTAEQALADADAADQAR
CCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
ASGDAAALAGLPLALKDIFCTQGVRTSCGSKMLDSFVAPYDATVVEKIRQAGGVSLGKTN
CCCCHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
MDEFAMGSSNENSFYGPVKNPWDLSAVPGGSSGGSAAAVAAGLVPAALGTDTGGSIRQPA
HHHHHCCCCCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCH
AFCGITGLKPTYGRVSRYGMVAYASSLDQGGPMARTAADCALLLETIAGHDVRDSTSVAR
HHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHH
VVPDYSAELDTSLAGLKIGLPKEYFGDGLDPEVETAVREAIKVYESLGAKVCEVSLPHTH
HCCCCHHHHCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCE
LAIPAYYVIAPAEASSNLSRYDGVRFGHRCEDPTDLEDLYKRTRAEGFGEEVKRRILIGT
EECCEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHEEE
HTLSEGFFDAYYLKAQQVRRLIRQDFLDAFDEVDVLMGPTAPTPAFDLGAQKDPVSMYLQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
DIYTIAVNLAGIPGISVPAGFAGNRPVGLQVLAPHFAEAQLLGVAHQFQQATDWHLKRPA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCCCC
FGKEMA
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA