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The map label for this gene is gacA [H]

Identifier: 92114254

GI number: 92114254

Start: 2400203

End: 2400847

Strand: Direct

Name: gacA [H]

Synonym: Csal_2132

Alternate gene names: 92114254

Gene position: 2400203-2400847 (Clockwise)

Preceding gene: 92114253

Following gene: 92114255

Centisome position: 64.93

GC content: 58.76

Gene sequence:

>645_bases
TTGATCAAGGTTCTTGTCGCTGACGATCACCATTTGGTACGTACTAGCATCGCCCGCATGCTCGACTCCGAGGACGGCTT
GAAGGTCATCGCCGAGGCCGTCGATGGCGAGGAGGCCGTCAACATGGCGCGTGAATTGCGCCCCGATATCGTGTTGATGG
ACATCCGCATGCCCGGCATCGGCGGGCTCGAAGCCACGCGCAAGATCATGCGCGGCATGTCGGATATCAAGGTCGTGATC
GTGACCGCCTTTCTGGAGGAGGCCTTTGCGCAACGCCTGATGGACGCCGGTGCCGCGGGCTTCATCAGCAAGGGCACGGA
GCTCGATGAAATGGTGCGTGCCATCCGCGCCATTTTCGATGGGCAGCGCTACATCAGCCCCGACATCGCGCAGAAAGTCG
TGCTTTCCAAGATGGGGACACAGGACAATCCCTTCGATCAACTCTCGCATCGCGAACTGCAGGTCGCGATGATGATCGTC
AATTGTCAGAAGGTCAGCGATATATCCGATCGCCTGTTCCTGAGCCCCAAGACGGTCAACACCTACCGCTACCGCATTTT
CGAGAAGCTCAACGTCCAGTCCGACGTCGAACTCACCCACCTCGGACTGCGCTATGGATTGATCGACGGCTACGAGGCGT
CGTGA

Upstream 100 bases:

>100_bases
CGAAACGCATCGCGACCTCCCATAATAACGTCACAATGGCCTTCGCCAGGGCCATCGAGGTCACCATTCCGCTAACCGGC
TATTGATTGCAGGAGTTCCC

Downstream 100 bases:

>100_bases
TACCAGGCATATCGGCATCGCAGGGCGCGCCGGGCCCCGTCCTCCTTTGATACACTGGCGGGCATGATCCATGCAACGAT
TCCCCCGCGCCCATGAGCTT

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MIKVLVADDHHLVRTSIARMLDSEDGLKVIAEAVDGEEAVNMARELRPDIVLMDIRMPGIGGLEATRKIMRGMSDIKVVI
VTAFLEEAFAQRLMDAGAAGFISKGTELDEMVRAIRAIFDGQRYISPDIAQKVVLSKMGTQDNPFDQLSHRELQVAMMIV
NCQKVSDISDRLFLSPKTVNTYRYRIFEKLNVQSDVELTHLGLRYGLIDGYEAS

Sequences:

>Translated_214_residues
MIKVLVADDHHLVRTSIARMLDSEDGLKVIAEAVDGEEAVNMARELRPDIVLMDIRMPGIGGLEATRKIMRGMSDIKVVI
VTAFLEEAFAQRLMDAGAAGFISKGTELDEMVRAIRAIFDGQRYISPDIAQKVVLSKMGTQDNPFDQLSHRELQVAMMIV
NCQKVSDISDRLFLSPKTVNTYRYRIFEKLNVQSDVELTHLGLRYGLIDGYEAS
>Mature_214_residues
MIKVLVADDHHLVRTSIARMLDSEDGLKVIAEAVDGEEAVNMARELRPDIVLMDIRMPGIGGLEATRKIMRGMSDIKVVI
VTAFLEEAFAQRLMDAGAAGFISKGTELDEMVRAIRAIFDGQRYISPDIAQKVVLSKMGTQDNPFDQLSHRELQVAMMIV
NCQKVSDISDRLFLSPKTVNTYRYRIFEKLNVQSDVELTHLGLRYGLIDGYEAS

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=212, Percent_Identity=52.8301886792453, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=36.1809045226131, Blast_Score=118, Evalue=3e-28,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=32.5242718446602, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=28.780487804878, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788521, Length=203, Percent_Identity=31.5270935960591, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1786599, Length=151, Percent_Identity=31.7880794701987, Blast_Score=61, Evalue=4e-11,
Organism=Escherichia coli, GI1788546, Length=202, Percent_Identity=22.2772277227723, Blast_Score=61, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=109, Percent_Identity=33.9449541284404, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23947; Mature: 23947

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVLVADDHHLVRTSIARMLDSEDGLKVIAEAVDGEEAVNMARELRPDIVLMDIRMPGI
CEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCC
GGLEATRKIMRGMSDIKVVIVTAFLEEAFAQRLMDAGAAGFISKGTELDEMVRAIRAIFD
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHC
GQRYISPDIAQKVVLSKMGTQDNPFDQLSHRELQVAMMIVNCQKVSDISDRLFLSPKTVN
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCH
TYRYRIFEKLNVQSDVELTHLGLRYGLIDGYEAS
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKVLVADDHHLVRTSIARMLDSEDGLKVIAEAVDGEEAVNMARELRPDIVLMDIRMPGI
CEEEEEECCCHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCC
GGLEATRKIMRGMSDIKVVIVTAFLEEAFAQRLMDAGAAGFISKGTELDEMVRAIRAIFD
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHHC
GQRYISPDIAQKVVLSKMGTQDNPFDQLSHRELQVAMMIVNCQKVSDISDRLFLSPKTVN
CCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCH
TYRYRIFEKLNVQSDVELTHLGLRYGLIDGYEAS
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]