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The map label for this gene is rnt

Identifier: 92114250

GI number: 92114250

Start: 2396817

End: 2397482

Strand: Direct

Name: rnt

Synonym: Csal_2128

Alternate gene names: 92114250

Gene position: 2396817-2397482 (Clockwise)

Preceding gene: 92114244

Following gene: 92114252

Centisome position: 64.84

GC content: 61.86

Gene sequence:

>666_bases
ATGAGCGACGGCCAGGCACGCGATTTGATGGCTCAACGTTTTCGAGGCTTTCTCCCGGTGGTGGTCGATCTGGAAACCGG
TGGCTTCAACGCCCAACGCGATGCCTTGCTCGAAATCGCCGCCGTGACCTTGACCATGGATGCCCAGGGCAACCTCATGC
CCGATGACACGCACGCCTTTCATGTGCGTCCCTTCGAGGGTGCCAATATCGAACAGGCAGCACTGGACTTCACCGGCATC
GACCTCGACAGTCCGCTGCGCCAGCAGGTCGCACTGAGCGAACACGACGCGCTCGGCGAGATCTTCAAGCCGGTCCGCAA
GGCTATCAAGGCCAATCAGTGCACGCGCGCCATACTCGTCGGTCACAACGCCGCTTTCGATCAGGGCTTTCTGAATGCCG
CCGTCGAGCGCAACGGCATCAAGCGCAATCCGTTTCATCCTTTTTCCTGCTTCGACACCGCGACGCTCGGCGGGCTGGTG
TATGGCCAGACCGTCCTGGCACGCGCCTGCCGAGCGGCGGGAATCGAGTTCGACAATGCCTCCGCCCACTCCGCGCGCTA
CGATACCGAACGCACCGCCGAGCTGTTCTGCGCCATGGTCAACCGCTACAAGAACCTGGGCGGCTGGGATCTGGCTTTGA
AGGAACAGGGCATGGACGAGGACTGA

Upstream 100 bases:

>100_bases
GGACGTCGGGGGGCGAAAATGCTACACTCGCTGCCACTTTGTCGCCCCCACCCAGGCGTGGCGACGCTTATCGAGACCGC
ATTGGGATGGGAGCAAAAGC

Downstream 100 bases:

>100_bases
CGCTCAGGTCCGACAAACGAAAGCCCCGCCGGTGTGAGCCGACGGGGCTTTCGCTATACCGCCCCCTCCCTTCGGAGCGG
GCCGCGGATCAAGGCTCCAC

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSDGQARDLMAQRFRGFLPVVVDLETGGFNAQRDALLEIAAVTLTMDAQGNLMPDDTHAFHVRPFEGANIEQAALDFTGI
DLDSPLRQQVALSEHDALGEIFKPVRKAIKANQCTRAILVGHNAAFDQGFLNAAVERNGIKRNPFHPFSCFDTATLGGLV
YGQTVLARACRAAGIEFDNASAHSARYDTERTAELFCAMVNRYKNLGGWDLALKEQGMDED

Sequences:

>Translated_221_residues
MSDGQARDLMAQRFRGFLPVVVDLETGGFNAQRDALLEIAAVTLTMDAQGNLMPDDTHAFHVRPFEGANIEQAALDFTGI
DLDSPLRQQVALSEHDALGEIFKPVRKAIKANQCTRAILVGHNAAFDQGFLNAAVERNGIKRNPFHPFSCFDTATLGGLV
YGQTVLARACRAAGIEFDNASAHSARYDTERTAELFCAMVNRYKNLGGWDLALKEQGMDED
>Mature_220_residues
SDGQARDLMAQRFRGFLPVVVDLETGGFNAQRDALLEIAAVTLTMDAQGNLMPDDTHAFHVRPFEGANIEQAALDFTGID
LDSPLRQQVALSEHDALGEIFKPVRKAIKANQCTRAILVGHNAAFDQGFLNAAVERNGIKRNPFHPFSCFDTATLGGLVY
GQTVLARACRAAGIEFDNASAHSARYDTERTAELFCAMVNRYKNLGGWDLALKEQGMDED

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain

Homologues:

Organism=Escherichia coli, GI1787941, Length=216, Percent_Identity=61.5740740740741, Blast_Score=270, Evalue=6e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNT_CHRSD (Q1QVM9)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574178.1
- ProteinModelPortal:   Q1QVM9
- SMR:   Q1QVM9
- STRING:   Q1QVM9
- GeneID:   4026464
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2128
- NMPDR:   fig|290398.4.peg.2262
- eggNOG:   COG0847
- HOGENOM:   HBG289562
- OMA:   YDAEKTA
- ProtClustDB:   PRK05168
- BioCyc:   CSAL290398:CSAL_2128-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00157
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337
- SMART:   SM00479
- TIGRFAMs:   TIGR01298

Pfam domain/function: PF00929 Exonuc_X-T; SSF53098 RNaseH_fold

EC number: 3.1.13.-

Molecular weight: Translated: 24190; Mature: 24059

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDGQARDLMAQRFRGFLPVVVDLETGGFNAQRDALLEIAAVTLTMDAQGNLMPDDTHAF
CCCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCEE
HVRPFEGANIEQAALDFTGIDLDSPLRQQVALSEHDALGEIFKPVRKAIKANQCTRAILV
EECCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GHNAAFDQGFLNAAVERNGIKRNPFHPFSCFDTATLGGLVYGQTVLARACRAAGIEFDNA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SAHSARYDTERTAELFCAMVNRYKNLGGWDLALKEQGMDED
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
SDGQARDLMAQRFRGFLPVVVDLETGGFNAQRDALLEIAAVTLTMDAQGNLMPDDTHAF
CCCHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHEEEEECCCCCCCCCCCCEE
HVRPFEGANIEQAALDFTGIDLDSPLRQQVALSEHDALGEIFKPVRKAIKANQCTRAILV
EECCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GHNAAFDQGFLNAAVERNGIKRNPFHPFSCFDTATLGGLVYGQTVLARACRAAGIEFDNA
CCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SAHSARYDTERTAELFCAMVNRYKNLGGWDLALKEQGMDED
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA