Click here to switch to the map view.

The map label for this gene is nth [H]

Identifier: 92114244

GI number: 92114244

Start: 2391987

End: 2392625

Strand: Direct

Name: nth [H]

Synonym: Csal_2122

Alternate gene names: 92114244

Gene position: 2391987-2392625 (Clockwise)

Preceding gene: 92114243

Following gene: 92114250

Centisome position: 64.71

GC content: 61.35

Gene sequence:

>639_bases
ATGAACGCTCAAAAACGCCACGAGATCTTTTCGCGCCTGCGCGACCACAATCCCACGCCGACGACGGAGCTGCATTGGCA
AACGCCATTCGAGCTGTTGACGGCAGTACTTCTTTCCGCCCAGGCCACGGACGTGGGAGTCAACAAGGCCACCGCGCGCC
TGTTCCCGGTAGCCAATACGCCGCAGGGCATCCTCGACCTGGGCCTGGAAGGTCTCAAGGACAAGATCAAGACCATCGGC
CTGTACAACAGCAAGGCCGATAACTTGATGAAGACCTGCCACCTGCTGCTCGAACGGCACGGCGGCGAGGTGCCGAATAC
CCGCGAGGCACTGGAAGCACTACCCGGTGTCGGCCGCAAGACCGCCAACGTGATTCTCAATACCGCCTTCGGGCAGCCGA
CCATGGCGGTGGATACGCATATCTTCCGGGTCGCCAACCGCACGCGCATCGCGCCAGGCAAGAACGTGCTCGAGGTCGAA
CAGAAACTGATGCGCCACGTCCCCAGGGAATTCCTGCACGATGCGCATCACTGGCTCATCCTTCACGGGCGCTACACCTG
CGTGGCTCGCAAGCCGCGTTGCGGAAGCTGCGTGATCGAGGATCTCTGCGAGTACAAGGACAAGACCGAACTGGCCTGA

Upstream 100 bases:

>100_bases
GCCAGCCGCGAGGCCGCCCACGGCCAGGTCGAAGAGGCCCAGCGCATGCTCGATGCTGCCCGTGCCGCCCTCGATTCCTC
TTCATCGCCTTGATTCGCTC

Downstream 100 bases:

>100_bases
CCCTTCTACGCACCTCGCAACACGTCGCGATACGCCTCACGCCACGCCGGCCATTGCCAGGCACGCGCATCGGGCAAGGC
GGGGCGCGTCGCCGGTTGAG

Product: endonuclease III / DNA-(apurinic or apyrimidinic site) lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNAQKRHEIFSRLRDHNPTPTTELHWQTPFELLTAVLLSAQATDVGVNKATARLFPVANTPQGILDLGLEGLKDKIKTIG
LYNSKADNLMKTCHLLLERHGGEVPNTREALEALPGVGRKTANVILNTAFGQPTMAVDTHIFRVANRTRIAPGKNVLEVE
QKLMRHVPREFLHDAHHWLILHGRYTCVARKPRCGSCVIEDLCEYKDKTELA

Sequences:

>Translated_212_residues
MNAQKRHEIFSRLRDHNPTPTTELHWQTPFELLTAVLLSAQATDVGVNKATARLFPVANTPQGILDLGLEGLKDKIKTIG
LYNSKADNLMKTCHLLLERHGGEVPNTREALEALPGVGRKTANVILNTAFGQPTMAVDTHIFRVANRTRIAPGKNVLEVE
QKLMRHVPREFLHDAHHWLILHGRYTCVARKPRCGSCVIEDLCEYKDKTELA
>Mature_212_residues
MNAQKRHEIFSRLRDHNPTPTTELHWQTPFELLTAVLLSAQATDVGVNKATARLFPVANTPQGILDLGLEGLKDKIKTIG
LYNSKADNLMKTCHLLLERHGGEVPNTREALEALPGVGRKTANVILNTAFGQPTMAVDTHIFRVANRTRIAPGKNVLEVE
QKLMRHVPREFLHDAHHWLILHGRYTCVARKPRCGSCVIEDLCEYKDKTELA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=8e-21,
Organism=Homo sapiens, GI115298648, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI6912520, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI190358497, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=5e-12,
Organism=Homo sapiens, GI115298654, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI115298652, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI115298650, Length=157, Percent_Identity=31.8471337579618, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1787920, Length=211, Percent_Identity=73.4597156398104, Blast_Score=333, Evalue=4e-93,
Organism=Escherichia coli, GI1789331, Length=129, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=30.8571428571429, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=24.4791666666667, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=178, Percent_Identity=30.8988764044944, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23795; Mature: 23795

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQKRHEIFSRLRDHNPTPTTELHWQTPFELLTAVLLSAQATDVGVNKATARLFPVANT
CCCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHEEECCCCC
PQGILDLGLEGLKDKIKTIGLYNSKADNLMKTCHLLLERHGGEVPNTREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
TANVILNTAFGQPTMAVDTHIFRVANRTRIAPGKNVLEVEQKLMRHVPREFLHDAHHWLI
HHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
LHGRYTCVARKPRCGSCVIEDLCEYKDKTELA
EECCEEEEECCCCCCHHHHHHHHHHCHHCCCC
>Mature Secondary Structure
MNAQKRHEIFSRLRDHNPTPTTELHWQTPFELLTAVLLSAQATDVGVNKATARLFPVANT
CCCHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCHHHEEECCCCC
PQGILDLGLEGLKDKIKTIGLYNSKADNLMKTCHLLLERHGGEVPNTREALEALPGVGRK
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCH
TANVILNTAFGQPTMAVDTHIFRVANRTRIAPGKNVLEVEQKLMRHVPREFLHDAHHWLI
HHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEE
LHGRYTCVARKPRCGSCVIEDLCEYKDKTELA
EECCEEEEECCCCCCHHHHHHHHHHCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]