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The map label for this gene is thiI [H]

Identifier: 92114240

GI number: 92114240

Start: 2386882

End: 2388372

Strand: Direct

Name: thiI [H]

Synonym: Csal_2118

Alternate gene names: 92114240

Gene position: 2386882-2388372 (Clockwise)

Preceding gene: 92114235

Following gene: 92114241

Centisome position: 64.57

GC content: 66.67

Gene sequence:

>1491_bases
ATGCGGTATCGCGTCAAGCTGTTCCCCGAGATCACCATCAAGTCAAGGCCCGTGCGCAAGGAGATGGTCCGCTGCCTGCG
CACCAACCTGCGCAACAGCCTTGCGCCCCTGGTGCCCGATGTACGCATCGCCGACTGCTGGGATGCGCTGGAGGTACGCA
TTCGCCGCCCCATCGACGAGACGACCCGCGAACGGGTGGAAGGCGTCTTGTCCCGCACCTCCGGCATTCACGAGACGCTG
GTGATCGAGGCCTATACGTTCACCTCTTTCGATGACACCGCCGCACACCTGGCGGCGTTATGGCAGGAGGCACTGGCAGG
CCAGCGCTTTCGGGTCAGCGTCAAGCGCCGAGGACAACATGACTTCACCTCGGCGGAGCTCGAGCGTTACCTGGGAGGCG
CCCTTCTTGCCGCCGCTCCGCAGGCCAAGGTGGATCTCACGCAGCCCGACGTGGATGTCACTCTCGAACTGCAGGACCAG
CATCTGCGCCTGGTCACACGGCGCCTTCCCGGCCTGGGCGGCTATCCGCTGGGTACCCAGGGTCAGGCGCTGGCGCTCAT
CTCGGGCGGCTACGATTCCCCGGTAGCCGCCTGGCGCATGATCCGCCGTGGCCTCAAGACCCACTACCTGTTCTTCAATC
TCGGCGGGGCCGCGCATGAACGCACCGTACGCGAGGTCGTCCATCGCGTGTGGCAAGGCTATAGCGCCTCTCATCGCGTG
CATTTCATCAGCGTGCCGTTCGAGGAGGTGCTCGACGAGATCCAGCGACGCGTGCCTGCCGGCCTGGCGGGGGTCGTGCT
CAAGCGGATGATGCTGCGCGCCGCCAATCGCGTTGCGGCGCGGGCACGCATTCCCGCCCTGATCACCGGGGATGCCCTGG
CCCAGGTCTCCAGCCAGAGCGTCACCAACCTGGGGCTGATCGATCGCGTCAGCGAGCGCCCCGTGCTGCGTCCGCTCATC
GCCGATGACAAGCAGCGCATCATCGAGGATGCTCAGCGCATCGGCACGGCCGAGCATGCCGAACGCCTGCCGGAATATTG
CGGCACGATTTCACGGCGCCCCAACACGCGCCCCAAGCTCGCCCAGATCGAGGCCGCCGAGGCCGATTTCGACATGAGCG
TCCTCGATGCCGCCCTGGAGGCCGCGAGTCGCACCCGGGTGGATCGCCTCCTCGATGCACCGCCGTCACGTCCGCTGGAT
GTTCCCGTGATCGCCTCCGCCGACGCACTGCGCGCAGCCGAGGACGTCACGGTGATCGACATTCGCCACCCCGATGAACG
CGATGCCGCACCGCTGAAGCTGCCGCATGGCGAGCCGCTGGCGATTCCGTTTTACGAACTCGCCGCACGCGCCGCGCAGC
TTTCAGGCCGGCAACGCTACGCGCTCTATTGCGCCCAGGGCGTGATGAGCAAGATGCAGGCGCTTCGGCTCGCCGACCAG
GGCCTCGACAACTTCCTCGTCTATCGGGACGACGCTCACGCCGAGCGCTGA

Upstream 100 bases:

>100_bases
GAGCATGATCGTCACGGCCCCCTGCGCCTCCGGGGACAACTGGCGAGCCCTCCGGCACCTTGCTACCATGGCGCGCCCAT
CGTCAGTCGACATGAATCAC

Downstream 100 bases:

>100_bases
ACCCGCGTCGCCGTCAACGCAAGGTCGCCATGCATTCACCCGTTCTCGCCATTCGCCGCACACCGCTGGTCGCCGTTTAT
GGCACGCTCAAGCGTGGTCT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MRYRVKLFPEITIKSRPVRKEMVRCLRTNLRNSLAPLVPDVRIADCWDALEVRIRRPIDETTRERVEGVLSRTSGIHETL
VIEAYTFTSFDDTAAHLAALWQEALAGQRFRVSVKRRGQHDFTSAELERYLGGALLAAAPQAKVDLTQPDVDVTLELQDQ
HLRLVTRRLPGLGGYPLGTQGQALALISGGYDSPVAAWRMIRRGLKTHYLFFNLGGAAHERTVREVVHRVWQGYSASHRV
HFISVPFEEVLDEIQRRVPAGLAGVVLKRMMLRAANRVAARARIPALITGDALAQVSSQSVTNLGLIDRVSERPVLRPLI
ADDKQRIIEDAQRIGTAEHAERLPEYCGTISRRPNTRPKLAQIEAAEADFDMSVLDAALEAASRTRVDRLLDAPPSRPLD
VPVIASADALRAAEDVTVIDIRHPDERDAAPLKLPHGEPLAIPFYELAARAAQLSGRQRYALYCAQGVMSKMQALRLADQ
GLDNFLVYRDDAHAER

Sequences:

>Translated_496_residues
MRYRVKLFPEITIKSRPVRKEMVRCLRTNLRNSLAPLVPDVRIADCWDALEVRIRRPIDETTRERVEGVLSRTSGIHETL
VIEAYTFTSFDDTAAHLAALWQEALAGQRFRVSVKRRGQHDFTSAELERYLGGALLAAAPQAKVDLTQPDVDVTLELQDQ
HLRLVTRRLPGLGGYPLGTQGQALALISGGYDSPVAAWRMIRRGLKTHYLFFNLGGAAHERTVREVVHRVWQGYSASHRV
HFISVPFEEVLDEIQRRVPAGLAGVVLKRMMLRAANRVAARARIPALITGDALAQVSSQSVTNLGLIDRVSERPVLRPLI
ADDKQRIIEDAQRIGTAEHAERLPEYCGTISRRPNTRPKLAQIEAAEADFDMSVLDAALEAASRTRVDRLLDAPPSRPLD
VPVIASADALRAAEDVTVIDIRHPDERDAAPLKLPHGEPLAIPFYELAARAAQLSGRQRYALYCAQGVMSKMQALRLADQ
GLDNFLVYRDDAHAER
>Mature_496_residues
MRYRVKLFPEITIKSRPVRKEMVRCLRTNLRNSLAPLVPDVRIADCWDALEVRIRRPIDETTRERVEGVLSRTSGIHETL
VIEAYTFTSFDDTAAHLAALWQEALAGQRFRVSVKRRGQHDFTSAELERYLGGALLAAAPQAKVDLTQPDVDVTLELQDQ
HLRLVTRRLPGLGGYPLGTQGQALALISGGYDSPVAAWRMIRRGLKTHYLFFNLGGAAHERTVREVVHRVWQGYSASHRV
HFISVPFEEVLDEIQRRVPAGLAGVVLKRMMLRAANRVAARARIPALITGDALAQVSSQSVTNLGLIDRVSERPVLRPLI
ADDKQRIIEDAQRIGTAEHAERLPEYCGTISRRPNTRPKLAQIEAAEADFDMSVLDAALEAASRTRVDRLLDAPPSRPLD
VPVIASADALRAAEDVTVIDIRHPDERDAAPLKLPHGEPLAIPFYELAARAAQLSGRQRYALYCAQGVMSKMQALRLADQ
GLDNFLVYRDDAHAER

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=489, Percent_Identity=43.558282208589, Blast_Score=417, Evalue=1e-118,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 55321; Mature: 55321

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS51165 THUMP ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYRVKLFPEITIKSRPVRKEMVRCLRTNLRNSLAPLVPDVRIADCWDALEVRIRRPIDE
CCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCH
TTRERVEGVLSRTSGIHETLVIEAYTFTSFDDTAAHLAALWQEALAGQRFRVSVKRRGQH
HHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCC
DFTSAELERYLGGALLAAAPQAKVDLTQPDVDVTLELQDQHLRLVTRRLPGLGGYPLGTQ
CCHHHHHHHHHCCHHHHCCCCCCEECCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCC
GQALALISGGYDSPVAAWRMIRRGLKTHYLFFNLGGAAHERTVREVVHRVWQGYSASHRV
CCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
HFISVPFEEVLDEIQRRVPAGLAGVVLKRMMLRAANRVAARARIPALITGDALAQVSSQS
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHH
VTNLGLIDRVSERPVLRPLIADDKQRIIEDAQRIGTAEHAERLPEYCGTISRRPNTRPKL
HHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCH
AQIEAAEADFDMSVLDAALEAASRTRVDRLLDAPPSRPLDVPVIASADALRAAEDVTVID
HHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHCCCEEEEE
IRHPDERDAAPLKLPHGEPLAIPFYELAARAAQLSGRQRYALYCAQGVMSKMQALRLADQ
ECCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GLDNFLVYRDDAHAER
CCCCEEEEECCCCCCC
>Mature Secondary Structure
MRYRVKLFPEITIKSRPVRKEMVRCLRTNLRNSLAPLVPDVRIADCWDALEVRIRRPIDE
CCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCH
TTRERVEGVLSRTSGIHETLVIEAYTFTSFDDTAAHLAALWQEALAGQRFRVSVKRRGQH
HHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHHHCCCEEEEEHHHCCCC
DFTSAELERYLGGALLAAAPQAKVDLTQPDVDVTLELQDQHLRLVTRRLPGLGGYPLGTQ
CCHHHHHHHHHCCHHHHCCCCCCEECCCCCCCEEEEECHHHHHHHHHHCCCCCCCCCCCC
GQALALISGGYDSPVAAWRMIRRGLKTHYLFFNLGGAAHERTVREVVHRVWQGYSASHRV
CCEEEEEECCCCCHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCEE
HFISVPFEEVLDEIQRRVPAGLAGVVLKRMMLRAANRVAARARIPALITGDALAQVSSQS
EEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHH
VTNLGLIDRVSERPVLRPLIADDKQRIIEDAQRIGTAEHAERLPEYCGTISRRPNTRPKL
HHHHHHHHHHCCCCCCCHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCH
AQIEAAEADFDMSVLDAALEAASRTRVDRLLDAPPSRPLDVPVIASADALRAAEDVTVID
HHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCHHHHHCCCEEEEE
IRHPDERDAAPLKLPHGEPLAIPFYELAARAAQLSGRQRYALYCAQGVMSKMQALRLADQ
ECCCCCCCCCCEECCCCCCCEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHC
GLDNFLVYRDDAHAER
CCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA