Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 92114235

GI number: 92114235

Start: 2382915

End: 2383664

Strand: Direct

Name: purN [H]

Synonym: Csal_2113

Alternate gene names: 92114235

Gene position: 2382915-2383664 (Clockwise)

Preceding gene: 92114234

Following gene: 92114240

Centisome position: 64.46

GC content: 65.2

Gene sequence:

>750_bases
ATGAACGACATGCCTCACGCCGATAACGCCGAAGCCGACACCAACGGCTTCACCCCCGAACCGGTGGACAAGCGTCGCGT
CGTGGTGCTGATTTCAGGCAACGGCAGCAACCTTCAGGCCCTGATCGATGCGCAACGACACGATGAGCTGGGCGGCGAGA
TCGTTGCCGTGATCTCCAATCGCGGCGACGCCTACGGCCTGGTGCGCGCCAAGGAAGCCGGCATCGACGCCGTGGTGTTG
CCCCATCAGGAATACGACGACCGCGAGGCCTACGACCGAGCGCTGATCAAGGTCATCGATCGCCATGCGCCCGACTTGAT
CGTCCTGGCCGGCTTCATGCGCATTCTCACCCCGATGTTCGTCCACCGCTATGCCGGTCGCGTGCTCAACATCCACCCCT
CGCTGCTCCCCGCGTATCAGGGGCTGCACACGCATCAGCGAGCGCTTGACGATGGCGTCGCCGAACATGGCGCCAGCGTG
CATTTCGTCACCGAGGAACTCGACGGCGGCCCGGTCGTGATGCAGGCCGTGGTCAAGGTCGGGGAGAACCAGTCCCTGGA
GACGTTGGTCGAGAAGGTCCAGGCCCGCGAGCACCTGATCTATCCCATCGCCGCGCGCTGGTTCCTCGAAGGCCGCCTGC
GGCTCGGCGCCGAGGGCGCCCTGTTCGACAACATGCCGCTGCCGCCCACCGGACTGCGGATGTCGCACGAGGATGCCGCG
GAAGAGCTCGACGAGGATATCGACGCGTAA

Upstream 100 bases:

>100_bases
AGGCCGCGCTCGAAGCGGCCGGCGAAACGGTGCATCGTCTCGGCGAGATCGTCCCGCGCCAGGACGAGGAAGAACAAGTC
CGATTGGAGAACGTCCGCTG

Downstream 100 bases:

>100_bases
ACGCTTGCGGAGACCACTGCAAAACGCCCCACGGCCGCTGGCCGTGGGGCGTTTTTCTTCCTGCGTCAGAGGCCTGGACA
GCCCCGACGGATACGCTTCC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNDMPHADNAEADTNGFTPEPVDKRRVVVLISGNGSNLQALIDAQRHDELGGEIVAVISNRGDAYGLVRAKEAGIDAVVL
PHQEYDDREAYDRALIKVIDRHAPDLIVLAGFMRILTPMFVHRYAGRVLNIHPSLLPAYQGLHTHQRALDDGVAEHGASV
HFVTEELDGGPVVMQAVVKVGENQSLETLVEKVQAREHLIYPIAARWFLEGRLRLGAEGALFDNMPLPPTGLRMSHEDAA
EELDEDIDA

Sequences:

>Translated_249_residues
MNDMPHADNAEADTNGFTPEPVDKRRVVVLISGNGSNLQALIDAQRHDELGGEIVAVISNRGDAYGLVRAKEAGIDAVVL
PHQEYDDREAYDRALIKVIDRHAPDLIVLAGFMRILTPMFVHRYAGRVLNIHPSLLPAYQGLHTHQRALDDGVAEHGASV
HFVTEELDGGPVVMQAVVKVGENQSLETLVEKVQAREHLIYPIAARWFLEGRLRLGAEGALFDNMPLPPTGLRMSHEDAA
EELDEDIDA
>Mature_249_residues
MNDMPHADNAEADTNGFTPEPVDKRRVVVLISGNGSNLQALIDAQRHDELGGEIVAVISNRGDAYGLVRAKEAGIDAVVL
PHQEYDDREAYDRALIKVIDRHAPDLIVLAGFMRILTPMFVHRYAGRVLNIHPSLLPAYQGLHTHQRALDDGVAEHGASV
HFVTEELDGGPVVMQAVVKVGENQSLETLVEKVQAREHLIYPIAARWFLEGRLRLGAEGALFDNMPLPPTGLRMSHEDAA
EELDEDIDA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=208, Percent_Identity=43.75, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI209869995, Length=208, Percent_Identity=43.75, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI209869993, Length=208, Percent_Identity=43.75, Blast_Score=176, Evalue=2e-44,
Organism=Escherichia coli, GI1788846, Length=205, Percent_Identity=57.5609756097561, Blast_Score=244, Evalue=4e-66,
Organism=Escherichia coli, GI1787483, Length=213, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=40.7821229050279, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320616, Length=197, Percent_Identity=35.0253807106599, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24582400, Length=190, Percent_Identity=44.7368421052632, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 27382; Mature: 27382

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMPHADNAEADTNGFTPEPVDKRRVVVLISGNGSNLQALIDAQRHDELGGEIVAVISN
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHCCCEEEEEEEC
RGDAYGLVRAKEAGIDAVVLPHQEYDDREAYDRALIKVIDRHAPDLIVLAGFMRILTPMF
CCCEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VHRYAGRVLNIHPSLLPAYQGLHTHQRALDDGVAEHGASVHFVTEELDGGPVVMQAVVKV
HHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
GENQSLETLVEKVQAREHLIYPIAARWFLEGRLRLGAEGALFDNMPLPPTGLRMSHEDAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHH
EELDEDIDA
HHHHHHCCC
>Mature Secondary Structure
MNDMPHADNAEADTNGFTPEPVDKRRVVVLISGNGSNLQALIDAQRHDELGGEIVAVISN
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHCCHHHHHCCCEEEEEEEC
RGDAYGLVRAKEAGIDAVVLPHQEYDDREAYDRALIKVIDRHAPDLIVLAGFMRILTPMF
CCCEEEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VHRYAGRVLNIHPSLLPAYQGLHTHQRALDDGVAEHGASVHFVTEELDGGPVVMQAVVKV
HHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHH
GENQSLETLVEKVQAREHLIYPIAARWFLEGRLRLGAEGALFDNMPLPPTGLRMSHEDAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEECCCCCCCCCCCCCHHHHH
EELDEDIDA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]