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The map label for this gene is fdhB1 [H]

Identifier: 92114039

GI number: 92114039

Start: 2177885

End: 2178535

Strand: Direct

Name: fdhB1 [H]

Synonym: Csal_1916

Alternate gene names: 92114039

Gene position: 2177885-2178535 (Clockwise)

Preceding gene: 92114038

Following gene: 92114040

Centisome position: 58.92

GC content: 62.83

Gene sequence:

>651_bases
ATGGCTCAAATGAAATTCCTTTGCGACGCCGAACGCTGCATCGAATGCAACGGCTGCGTCACGGCCTGCAAGAACGCCAA
CGAGACCGACTGGGGCATCCAGCGTCGTCGCGTGGTCACCCTGGACGACGGCATGCCCTCGGAGGTCTCGATTTCCGTGG
CCTGCATGCACTGCGACGACGCCCCGTGCATGGCGGTGTGCCCGACCGACTGTTTCTACAAGACCGATGACGGCATCGTC
CTGCACGACAAGGACATCTGCATCGGCTGCGGTTACTGCCTCTACGCCTGCCCCTTCGGCGCGCCGCAGTTTCCCAAGGA
AGAGGCATTCGGCGAGCGCGGCAAGATGGACAAGTGCACCTTCTGCGCCGGCGGTCCCGCCGAGACCAAGGAAGAGGAGT
ACGAGAAATACGGCGCCAACCGCATTGCCGAGGGCAAGCTGCCGCTATGCGCCGAGATGTGCTCCACCAAGGCGTTGCTC
GCCGGTGATGCACAGACCATTTCCGACATCTTTCGTCAGCGAGTGGTCTATCGCGGCCACGAGGACGGCGGCTGGTCGTC
GGTCGAAAAAGCGCAGAATGCGGGCCCAGATGTCTCGGCCAATCTCGACGAGATGGCCTACGACGCCACCCGCGCCACAC
GTGAAGGGTGA

Upstream 100 bases:

>100_bases
CCAAGTGCACCCTGTGCCGGATCGAAAAAGCCTCCGCCTGACCCGACGATGCTTGAAACGCCGATGAGGCCACGCGCCTC
ATCGGCAAGAGGAGAACACC

Downstream 100 bases:

>100_bases
GGAGAGCGCTCATGCACACACTCATGCAATGCCTATGGCGGCTGCGTATGATCGGTGCGGTGCTGTTGCTGGCGGTGCTG
GCCGCCGGCCTGGCCCCACC

Product: iron-sulfur binding 4Fe-4S ferredoxin

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MAQMKFLCDAERCIECNGCVTACKNANETDWGIQRRRVVTLDDGMPSEVSISVACMHCDDAPCMAVCPTDCFYKTDDGIV
LHDKDICIGCGYCLYACPFGAPQFPKEEAFGERGKMDKCTFCAGGPAETKEEEYEKYGANRIAEGKLPLCAEMCSTKALL
AGDAQTISDIFRQRVVYRGHEDGGWSSVEKAQNAGPDVSANLDEMAYDATRATREG

Sequences:

>Translated_216_residues
MAQMKFLCDAERCIECNGCVTACKNANETDWGIQRRRVVTLDDGMPSEVSISVACMHCDDAPCMAVCPTDCFYKTDDGIV
LHDKDICIGCGYCLYACPFGAPQFPKEEAFGERGKMDKCTFCAGGPAETKEEEYEKYGANRIAEGKLPLCAEMCSTKALL
AGDAQTISDIFRQRVVYRGHEDGGWSSVEKAQNAGPDVSANLDEMAYDATRATREG
>Mature_215_residues
AQMKFLCDAERCIECNGCVTACKNANETDWGIQRRRVVTLDDGMPSEVSISVACMHCDDAPCMAVCPTDCFYKTDDGIVL
HDKDICIGCGYCLYACPFGAPQFPKEEAFGERGKMDKCTFCAGGPAETKEEEYEKYGANRIAEGKLPLCAEMCSTKALLA
GDAQTISDIFRQRVVYRGHEDGGWSSVEKAQNAGPDVSANLDEMAYDATRATREG

Specific function: This chain is an electron transfer unit containing 18 cysteine residues, 16 of which occur in four clusters [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787872, Length=177, Percent_Identity=34.4632768361582, Blast_Score=104, Evalue=4e-24,
Organism=Escherichia coli, GI1787122, Length=172, Percent_Identity=34.3023255813954, Blast_Score=100, Evalue=8e-23,
Organism=Escherichia coli, GI2367345, Length=179, Percent_Identity=32.9608938547486, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789370, Length=201, Percent_Identity=26.3681592039801, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510944, Length=182, Percent_Identity=31.3186813186813, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787749, Length=207, Percent_Identity=27.536231884058, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1790326, Length=202, Percent_Identity=26.2376237623762, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87082114, Length=89, Percent_Identity=39.3258426966292, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082180, Length=178, Percent_Identity=30.3370786516854, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1788811, Length=132, Percent_Identity=30.3030303030303, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI2367245, Length=171, Percent_Identity=30.4093567251462, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789079, Length=201, Percent_Identity=27.363184079602, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23586; Mature: 23455

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

8.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
12.0 %Cys+Met (Translated Protein)
8.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
11.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQMKFLCDAERCIECNGCVTACKNANETDWGIQRRRVVTLDDGMPSEVSISVACMHCDD
CCCCCHHHCHHHHEECCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEECCC
APCMAVCPTDCFYKTDDGIVLHDKDICIGCGYCLYACPFGAPQFPKEEAFGERGKMDKCT
CCEEEECCCCCEEECCCCEEEECCCEEEECCHHHHHCCCCCCCCCCHHHHCCCCCCCCEE
FCAGGPAETKEEEYEKYGANRIAEGKLPLCAEMCSTKALLAGDAQTISDIFRQRVVYRGH
EECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCHHEECCCHHHHHHHHHHHHHHCCC
EDGGWSSVEKAQNAGPDVSANLDEMAYDATRATREG
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AQMKFLCDAERCIECNGCVTACKNANETDWGIQRRRVVTLDDGMPSEVSISVACMHCDD
CCCCHHHCHHHHEECCCHHHHHCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEEECCC
APCMAVCPTDCFYKTDDGIVLHDKDICIGCGYCLYACPFGAPQFPKEEAFGERGKMDKCT
CCEEEECCCCCEEECCCCEEEECCCEEEECCHHHHHCCCCCCCCCCHHHHCCCCCCCCEE
FCAGGPAETKEEEYEKYGANRIAEGKLPLCAEMCSTKALLAGDAQTISDIFRQRVVYRGH
EECCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHCCHHEECCCHHHHHHHHHHHHHHCCC
EDGGWSSVEKAQNAGPDVSANLDEMAYDATRATREG
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1781728; 14500908 [H]