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The map label for this gene is mpl [H]

Identifier: 92113659

GI number: 92113659

Start: 1747838

End: 1749208

Strand: Direct

Name: mpl [H]

Synonym: Csal_1535

Alternate gene names: 92113659

Gene position: 1747838-1749208 (Clockwise)

Preceding gene: 92113657

Following gene: 92113660

Centisome position: 47.28

GC content: 69.29

Gene sequence:

>1371_bases
ATGCACGTCCATATTCTAGGGGTTTGCGGTACGTTCATGGGGAGTCTGGCGCGGTTGGCCAAGGCGCTCGGACATCAGGT
CAGCGGTTCGGATGCCAACGTCTATCCGCCGATGAGCACTCAGCTCGAAGAGGCGGGCATCGCCCTTTGCGAGGGCTATG
CCGCCGACAACCTGATGCCGCGTCCCGATCTCGTGATCATCGGCAATGCATTGTCGCGCGGCAACCCCGAGGTGGAGGCC
GTGCTCAATGCCAAATTGCCCTACGTCTCGGGCCCGCAGTGGCTGGCCGAGCAGGTGCTGCCGGGCCGGCGCGTGGTGGC
GGTGGCCGGCACTCACGGCAAGACCACGACCGCCAGTCTGCTGACGTGGATTCTGGAGGCGGCCGGCCAGGCGCCGGGGT
TCTTGATCGGCGGGGTGCCGCGCAATCTGGGCGTGTCCTCGCGACTGGGAGAGCCCGGGGCGCCGTTCGTGGTCGAGGCC
GATGAGTACGACACGGCCTTCTTCGACAAGCGTTCCAAGTTCGTCCACTACCGCCCGGATATCGCGGTGCTGGGCAACCT
CGAGTTCGATCACGCGGACATCTTTCCCGACCTGGCCGCCATCGAGCGTCAGTTCCACCATCTGGTACGTACCGTCCCCG
GGCAAGGGCAACTGGTGGTGGCGGCGGGCGAGCCCGCCCTCGAGCGCGTGCTGGCGCAAGGCTGCTGGAGCCCCGTCGTG
CGTTTCGGTGCCAGCGACGACGCCTCATGGCAGTGGCGGCTGGAAAGCGCCGATGGCCGTCGCTTCGCGGTGCGTCATGG
CGAGCGCGTCGCGCACGTCGACTGGGCGCTGAGCGGGCGTCACAACGTCGCCAACGCGGTGGCGGCCCTGGCTGCGGCGT
CGGCCTGCGGTGTCGCCCTCGAGGCAGGGGCGGCGGCGCTGGCACGCTTCGAATCGCCGCGCCGACGCCAGGAGGTGCGA
GGCGAGGTCGGCGGCATTCAGGTCATCGACGACTTCGCCCATCATCCCACGGCCATCGCCGCCACGCTCGAGGGGCTGGC
AGCGGCGACCGACGCGGGGCGGCTGCTGGCGGTGATCGAACCGCGTTCCAATACGATGCGTCTGGGCAGCATGCGCGAGC
GCTTGCCGGACAGCGTGGCGGCGGCGGACCGCGTCTGGTGGTATCGGCCACCGGAGGTCGAATGGTCGCTGGAGGCGCTT
GCGGCGGCCTGTCCGGTGCCGGCGACGGTGAGCGACGAGATGCCGGCCCTGGTCGCGGCCATCGTGGCCGAGGCGCGTCC
GGGAGACCGGATCGTGGTGATGTCGAACGGGGCGTTCGGCGGTATCCACGAAAAGTTGCTGGCGGCACTGGAGGCCCGGC
ATGGCGGATGA

Upstream 100 bases:

>100_bases
ATGATAGCCGACATCGACGGTCGATGCCTATGCCAGGGCGCTCGGCTGGTGGGTCGCGCGGCGCCATGCTAGGCTCGCCG
CATCATGGCAGGAGGCTCAG

Downstream 100 bases:

>100_bases
AGCAATGTTCGAAGCGCCTGTCACCGTGGCGTTGACGGGGGCATCCGGGGCGCAGTACGGGCTGCGGCTCATCGACTGCC
TGGTGGCGGCGCGGCATCAG

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MHVHILGVCGTFMGSLARLAKALGHQVSGSDANVYPPMSTQLEEAGIALCEGYAADNLMPRPDLVIIGNALSRGNPEVEA
VLNAKLPYVSGPQWLAEQVLPGRRVVAVAGTHGKTTTASLLTWILEAAGQAPGFLIGGVPRNLGVSSRLGEPGAPFVVEA
DEYDTAFFDKRSKFVHYRPDIAVLGNLEFDHADIFPDLAAIERQFHHLVRTVPGQGQLVVAAGEPALERVLAQGCWSPVV
RFGASDDASWQWRLESADGRRFAVRHGERVAHVDWALSGRHNVANAVAALAAASACGVALEAGAAALARFESPRRRQEVR
GEVGGIQVIDDFAHHPTAIAATLEGLAAATDAGRLLAVIEPRSNTMRLGSMRERLPDSVAAADRVWWYRPPEVEWSLEAL
AAACPVPATVSDEMPALVAAIVAEARPGDRIVVMSNGAFGGIHEKLLAALEARHGG

Sequences:

>Translated_456_residues
MHVHILGVCGTFMGSLARLAKALGHQVSGSDANVYPPMSTQLEEAGIALCEGYAADNLMPRPDLVIIGNALSRGNPEVEA
VLNAKLPYVSGPQWLAEQVLPGRRVVAVAGTHGKTTTASLLTWILEAAGQAPGFLIGGVPRNLGVSSRLGEPGAPFVVEA
DEYDTAFFDKRSKFVHYRPDIAVLGNLEFDHADIFPDLAAIERQFHHLVRTVPGQGQLVVAAGEPALERVLAQGCWSPVV
RFGASDDASWQWRLESADGRRFAVRHGERVAHVDWALSGRHNVANAVAALAAASACGVALEAGAAALARFESPRRRQEVR
GEVGGIQVIDDFAHHPTAIAATLEGLAAATDAGRLLAVIEPRSNTMRLGSMRERLPDSVAAADRVWWYRPPEVEWSLEAL
AAACPVPATVSDEMPALVAAIVAEARPGDRIVVMSNGAFGGIHEKLLAALEARHGG
>Mature_456_residues
MHVHILGVCGTFMGSLARLAKALGHQVSGSDANVYPPMSTQLEEAGIALCEGYAADNLMPRPDLVIIGNALSRGNPEVEA
VLNAKLPYVSGPQWLAEQVLPGRRVVAVAGTHGKTTTASLLTWILEAAGQAPGFLIGGVPRNLGVSSRLGEPGAPFVVEA
DEYDTAFFDKRSKFVHYRPDIAVLGNLEFDHADIFPDLAAIERQFHHLVRTVPGQGQLVVAAGEPALERVLAQGCWSPVV
RFGASDDASWQWRLESADGRRFAVRHGERVAHVDWALSGRHNVANAVAALAAASACGVALEAGAAALARFESPRRRQEVR
GEVGGIQVIDDFAHHPTAIAATLEGLAAATDAGRLLAVIEPRSNTMRLGSMRERLPDSVAAADRVWWYRPPEVEWSLEAL
AAACPVPATVSDEMPALVAAIVAEARPGDRIVVMSNGAFGGIHEKLLAALEARHGG

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=455, Percent_Identity=55.1648351648352, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1786279, Length=378, Percent_Identity=30.6878306878307, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 48382; Mature: 48382

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVHILGVCGTFMGSLARLAKALGHQVSGSDANVYPPMSTQLEEAGIALCEGYAADNLMP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCCC
RPDLVIIGNALSRGNPEVEAVLNAKLPYVSGPQWLAEQVLPGRRVVAVAGTHGKTTTASL
CCCEEEEECHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHH
LTWILEAAGQAPGFLIGGVPRNLGVSSRLGEPGAPFVVEADEYDTAFFDKRSKFVHYRPD
HHHHHHHCCCCCCEEECCCCCCCCCHHCCCCCCCCEEEECCCCCHHHHHCCCCEEEECCC
IAVLGNLEFDHADIFPDLAAIERQFHHLVRTVPGQGQLVVAAGEPALERVLAQGCWSPVV
EEEEECCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
RFGASDDASWQWRLESADGRRFAVRHGERVAHVDWALSGRHNVANAVAALAAASACGVAL
HCCCCCCCCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
EAGAAALARFESPRRRQEVRGEVGGIQVIDDFAHHPTAIAATLEGLAAATDAGRLLAVIE
HHHHHHHHHHCCHHHHHHHCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEC
PRSNTMRLGSMRERLPDSVAAADRVWWYRPPEVEWSLEALAAACPVPATVSDEMPALVAA
CCCCCEEHHHHHHHCCHHHHHHHCEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
IVAEARPGDRIVVMSNGAFGGIHEKLLAALEARHGG
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MHVHILGVCGTFMGSLARLAKALGHQVSGSDANVYPPMSTQLEEAGIALCEGYAADNLMP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCCCCCCCC
RPDLVIIGNALSRGNPEVEAVLNAKLPYVSGPQWLAEQVLPGRRVVAVAGTHGKTTTASL
CCCEEEEECHHCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHH
LTWILEAAGQAPGFLIGGVPRNLGVSSRLGEPGAPFVVEADEYDTAFFDKRSKFVHYRPD
HHHHHHHCCCCCCEEECCCCCCCCCHHCCCCCCCCEEEECCCCCHHHHHCCCCEEEECCC
IAVLGNLEFDHADIFPDLAAIERQFHHLVRTVPGQGQLVVAAGEPALERVLAQGCWSPVV
EEEEECCCCCHHHHCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHH
RFGASDDASWQWRLESADGRRFAVRHGERVAHVDWALSGRHNVANAVAALAAASACGVAL
HCCCCCCCCEEEEEECCCCCEEEEECCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
EAGAAALARFESPRRRQEVRGEVGGIQVIDDFAHHPTAIAATLEGLAAATDAGRLLAVIE
HHHHHHHHHHCCHHHHHHHCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHCCCCCEEEEEC
PRSNTMRLGSMRERLPDSVAAADRVWWYRPPEVEWSLEALAAACPVPATVSDEMPALVAA
CCCCCEEHHHHHHHCCHHHHHHHCEEECCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHH
IVAEARPGDRIVVMSNGAFGGIHEKLLAALEARHGG
HHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]