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The map label for this gene is btuF [H]

Identifier: 92113616

GI number: 92113616

Start: 1692167

End: 1693021

Strand: Direct

Name: btuF [H]

Synonym: Csal_1492

Alternate gene names: 92113616

Gene position: 1692167-1693021 (Clockwise)

Preceding gene: 92113614

Following gene: 92113619

Centisome position: 45.78

GC content: 72.28

Gene sequence:

>855_bases
ATGCTCGCATGGTTCAGCGTCGCGTCGCTTTTCAGTGCGTCGGCGATGGCAGCGCTCGAAGTCGACGACGACGCGGGCCG
TACGGTGGCGTTGACGGCGCCCGCCGAGCGTGTCGTGGCACTGGCGCCGCACATCGTGGAGCTGCTGTACGCGGTGGGGG
CCGGCGAGCGCGTGGTGGGGGCCCTAGAGGGCAGCGATTTTCCACGCGCCGCGCGGGACATCCCTCGGGTGGGCAGTTAC
CGAGGCATCGCCCTGGAAGCCATCATGGCGCGCGAGCCGGACCTGGTGGTGACCTGGCGCAGTGGCACGCCGCGCAGCGT
GGTGACGCGCCTCGAGGCGCTGGGCGTGCCGGTCTACGCCAGCGAGCCGCGGCGACTCGCCGACGTGGCTGGCGACCTGC
GCGACCTGGGGCGTCTGACCGGTCACGCCGAGGCAGGCGCGCGTGCGGCCGAGGCGTTCGAGGCACGCCTCGCGGCCGCT
CGGCAACGCCTGCCCGAAACGCCGCGCGTGTTCTATCAACTGGGGCGCCACCCGCTGACCACGCTGGGCGACGGCCACAT
CGTCACCCGGGCGATTCGCCATTGCGGCGGGGAGCCGCTGTTCGCGGACCGCCGGGTCCTGGTGCCTGAAATCGGTCGCG
AAGCCTTGATCGAGGCGCGCCCTGACGTGATTCTCGCGGCCGCGCCGGACGATGACTGGCAGCAGGCCTGGCAGGACGAC
ACCCTGCTGCCGGCGGTGCGCGAGGGCCATCTGTACACTCTCGATCCCGATGCCATCAGTCGCCCCGGACCGCGCCTGAT
CCAGGGCGTCGAGCAGGTCTGCCGGGCGCTCGAGCGGGCGACGCCACCGTCCTGA

Upstream 100 bases:

>100_bases
GGGCGAGCGACCCATCGGGACATGCTATCCTGCGCGTTCGTCAGTGGCTAATGGAGTCGTGAGATTGCCGCGTCTTTCCC
CGTTTCTTGCACATGTCGTG

Downstream 100 bases:

>100_bases
CCGGGCGGCTCAGTAGTCGATGCCGCGACGTGCCTGAACGCCGGCATTGAAGGCGTGGCGCACTTCCTGCATCTCGGTGA
CGGTATCGGCCATGTCGAGC

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLAWFSVASLFSASAMAALEVDDDAGRTVALTAPAERVVALAPHIVELLYAVGAGERVVGALEGSDFPRAARDIPRVGSY
RGIALEAIMAREPDLVVTWRSGTPRSVVTRLEALGVPVYASEPRRLADVAGDLRDLGRLTGHAEAGARAAEAFEARLAAA
RQRLPETPRVFYQLGRHPLTTLGDGHIVTRAIRHCGGEPLFADRRVLVPEIGREALIEARPDVILAAAPDDDWQQAWQDD
TLLPAVREGHLYTLDPDAISRPGPRLIQGVEQVCRALERATPPS

Sequences:

>Translated_284_residues
MLAWFSVASLFSASAMAALEVDDDAGRTVALTAPAERVVALAPHIVELLYAVGAGERVVGALEGSDFPRAARDIPRVGSY
RGIALEAIMAREPDLVVTWRSGTPRSVVTRLEALGVPVYASEPRRLADVAGDLRDLGRLTGHAEAGARAAEAFEARLAAA
RQRLPETPRVFYQLGRHPLTTLGDGHIVTRAIRHCGGEPLFADRRVLVPEIGREALIEARPDVILAAAPDDDWQQAWQDD
TLLPAVREGHLYTLDPDAISRPGPRLIQGVEQVCRALERATPPS
>Mature_284_residues
MLAWFSVASLFSASAMAALEVDDDAGRTVALTAPAERVVALAPHIVELLYAVGAGERVVGALEGSDFPRAARDIPRVGSY
RGIALEAIMAREPDLVVTWRSGTPRSVVTRLEALGVPVYASEPRRLADVAGDLRDLGRLTGHAEAGARAAEAFEARLAAA
RQRLPETPRVFYQLGRHPLTTLGDGHIVTRAIRHCGGEPLFADRRVLVPEIGREALIEARPDVILAAAPDDDWQQAWQDD
TLLPAVREGHLYTLDPDAISRPGPRLIQGVEQVCRALERATPPS

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=250, Percent_Identity=31.6, Blast_Score=127, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 30616; Mature: 30616

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAWFSVASLFSASAMAALEVDDDAGRTVALTAPAERVVALAPHIVELLYAVGAGERVVG
CCHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEE
ALEGSDFPRAARDIPRVGSYRGIALEAIMAREPDLVVTWRSGTPRSVVTRLEALGVPVYA
ECCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEC
SEPRRLADVAGDLRDLGRLTGHAEAGARAAEAFEARLAAARQRLPETPRVFYQLGRHPLT
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
TLGDGHIVTRAIRHCGGEPLFADRRVLVPEIGREALIEARPDVILAAAPDDDWQQAWQDD
CCCCCHHHHHHHHHCCCCCCCCCCEEHHHHCCHHHHHHCCCCEEEEECCCHHHHHHHCCC
TLLPAVREGHLYTLDPDAISRPGPRLIQGVEQVCRALERATPPS
CCCHHHHCCCEEEECHHHHCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLAWFSVASLFSASAMAALEVDDDAGRTVALTAPAERVVALAPHIVELLYAVGAGERVVG
CCHHHHHHHHHHHHHHEEEEECCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCEEE
ALEGSDFPRAARDIPRVGSYRGIALEAIMAREPDLVVTWRSGTPRSVVTRLEALGVPVYA
ECCCCCCCHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCEEC
SEPRRLADVAGDLRDLGRLTGHAEAGARAAEAFEARLAAARQRLPETPRVFYQLGRHPLT
CCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
TLGDGHIVTRAIRHCGGEPLFADRRVLVPEIGREALIEARPDVILAAAPDDDWQQAWQDD
CCCCCHHHHHHHHHCCCCCCCCCCEEHHHHCCHHHHHHCCCCEEEEECCCHHHHHHHCCC
TLLPAVREGHLYTLDPDAISRPGPRLIQGVEQVCRALERATPPS
CCCHHHHCCCEEEECHHHHCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]