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The map label for this gene is mboIAM [H]

Identifier: 92113527

GI number: 92113527

Start: 1589689

End: 1590450

Strand: Direct

Name: mboIAM [H]

Synonym: Csal_1401

Alternate gene names: 92113527

Gene position: 1589689-1590450 (Clockwise)

Preceding gene: 92113526

Following gene: 92113529

Centisome position: 43.0

GC content: 59.32

Gene sequence:

>762_bases
ATGACCATGAAACCGATCCTCCCATGGATGGGAGGCAAGCGCCGTCTTGCGAAAGCCATCATCCCCCAGTTCCAGCCACA
CAGAACCTATGTGGAACCCTTCTGCGGGGGAGCGGCCATCTTCTTCTTGAAAGATCCGAGCCCCGTGGAGGTGATCAATG
ATGCCCATGGGGAGTTGGTGAATCTCTATCGCATCGTCAAGCACCACCCCGACGAGCTGGTGCGCCAGTTCCGCTGGAGC
CTGGTAAGCCGGGAAGAGTACCTGACTCAGCGTGAAGTGAACCCGCGGCACCTCACTGATATTCAGCGCGCGGCACGCTT
CTTCTACCTGCAGAAGCTGGCCTTCGGTGGCAAGGTACATGGCCAGACCTTTGGGACGTCAGCGACCAGCCCACCTCGTC
TCAACCTGATGCGCATTGAGGAGGATTTGAGCCAGGCACACCTCCGGCTATCTCGCACGGTGATCGAGCATCTAGACTGG
TCGGAATGCGTGACGCGCTACGATCGGCCCGAGACGCTCTTCTACCTGGATCCGCCGTACTGGGAAACCGCCGGCTACGG
CACCGACTTCGGCCTCGAGCAGTACGAGCGAATGGCCGAGCTGGCACGGAAAGCAAAAGGGCAGGTCGTCATTAGCGTCA
ACGACATCCCGGAGATGCGGCGAGCGTTCGCGGGCCTCAAGATCCACACCACCCAGATCAATTACACCGTCAGCAAGCAA
TCAACTGGAAAGCGGGGAGAGCTGATCATCACCAACCGCTGA

Upstream 100 bases:

>100_bases
GCATGCATCGCCGTGATCGTCCCTGATCGTAAACGCCTTCCTGGCGCATCCAGCGACACCGAAAGCATATCAGAAACTGG
ATAAATAAACAGGTGTCAGG

Downstream 100 bases:

>100_bases
ATGGTGTGTAAATTGAAAAAGGGTCGCCCGATTGGCGACCCCTTCTTCATCCCTTGGAGCTATGTGGATCAGAGCTTCCG
GGATAGGTGCGTTCCTCTTG

Product: DNA adenine methylase

Products: NA

Alternate protein names: M.MboIA; Adenine-specific methyltransferase MboIA [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTMKPILPWMGGKRRLAKAIIPQFQPHRTYVEPFCGGAAIFFLKDPSPVEVINDAHGELVNLYRIVKHHPDELVRQFRWS
LVSREEYLTQREVNPRHLTDIQRAARFFYLQKLAFGGKVHGQTFGTSATSPPRLNLMRIEEDLSQAHLRLSRTVIEHLDW
SECVTRYDRPETLFYLDPPYWETAGYGTDFGLEQYERMAELARKAKGQVVISVNDIPEMRRAFAGLKIHTTQINYTVSKQ
STGKRGELIITNR

Sequences:

>Translated_253_residues
MTMKPILPWMGGKRRLAKAIIPQFQPHRTYVEPFCGGAAIFFLKDPSPVEVINDAHGELVNLYRIVKHHPDELVRQFRWS
LVSREEYLTQREVNPRHLTDIQRAARFFYLQKLAFGGKVHGQTFGTSATSPPRLNLMRIEEDLSQAHLRLSRTVIEHLDW
SECVTRYDRPETLFYLDPPYWETAGYGTDFGLEQYERMAELARKAKGQVVISVNDIPEMRRAFAGLKIHTTQINYTVSKQ
STGKRGELIITNR
>Mature_252_residues
TMKPILPWMGGKRRLAKAIIPQFQPHRTYVEPFCGGAAIFFLKDPSPVEVINDAHGELVNLYRIVKHHPDELVRQFRWSL
VSREEYLTQREVNPRHLTDIQRAARFFYLQKLAFGGKVHGQTFGTSATSPPRLNLMRIEEDLSQAHLRLSRTVIEHLDWS
ECVTRYDRPETLFYLDPPYWETAGYGTDFGLEQYERMAELARKAKGQVVISVNDIPEMRRAFAGLKIHTTQINYTVSKQS
TGKRGELIITNR

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=257, Percent_Identity=25.2918287937743, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 29301; Mature: 29170

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMKPILPWMGGKRRLAKAIIPQFQPHRTYVEPFCGGAAIFFLKDPSPVEVINDAHGELV
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHCCHHHHH
NLYRIVKHHPDELVRQFRWSLVSREEYLTQREVNPRHLTDIQRAARFFYLQKLAFGGKVH
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
GQTFGTSATSPPRLNLMRIEEDLSQAHLRLSRTVIEHLDWSECVTRYDRPETLFYLDPPY
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCC
WETAGYGTDFGLEQYERMAELARKAKGQVVISVNDIPEMRRAFAGLKIHTTQINYTVSKQ
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCEEEEEEEEEEEECC
STGKRGELIITNR
CCCCCCCEEEECC
>Mature Secondary Structure 
TMKPILPWMGGKRRLAKAIIPQFQPHRTYVEPFCGGAAIFFLKDPSPVEVINDAHGELV
CCCCCCCCCCCHHHHHHHHCCCCCCCCCHHCCCCCCEEEEEEECCCCHHHHHCCHHHHH
NLYRIVKHHPDELVRQFRWSLVSREEYLTQREVNPRHLTDIQRAARFFYLQKLAFGGKVH
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEC
GQTFGTSATSPPRLNLMRIEEDLSQAHLRLSRTVIEHLDWSECVTRYDRPETLFYLDPPY
CCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCEEEEECCCC
WETAGYGTDFGLEQYERMAELARKAKGQVVISVNDIPEMRRAFAGLKIHTTQINYTVSKQ
CCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHCCCEEEEEEEEEEEECC
STGKRGELIITNR
CCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]