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The map label for this gene is queF

Identifier: 92113479

GI number: 92113479

Start: 1551257

End: 1552090

Strand: Direct

Name: queF

Synonym: Csal_1353

Alternate gene names: 92113479

Gene position: 1551257-1552090 (Clockwise)

Preceding gene: 92113478

Following gene: 92113480

Centisome position: 41.96

GC content: 67.51

Gene sequence:

>834_bases
ATGAGCGAACGTGACAAAACCCTCGAGCATGCGCCGCTGGGTCGCGAGTCCGCGTATCCCGAACACTACGACGCCGGCCT
GCTGTTTCCCATTCCCCGGCAGGCCAACCGCGCGCCGCTGGGGCTCGATGATGCCGCGCTGCCGTTCGAGGGCGAGGATG
AGTGGCACGCCTTCGAGCTTTCCTGGCTCGACGCGAAGGGCAAGCCGATCGTCGCCGTGGCCCGGTTCCGGCTGCCCGCC
GACTCCCCCTCGCTGATCGAGTCGAAGTCCTGGAAGCTGTACCTCAACAGCTTCAACCAGACGCGCTTCGACAGACGCGA
GGCGGTGATCGACACGCTCGAGCGTGACCTGGCCCAGGCGGCGGGCGCGTCGGTGGCGGTGGCCCTGTTCGGCGTCGAGG
ACGATGCCCTGATGCCCCGAGCGCTGCCCGGCGAGTGCCTGGACGATCTCGACGTGAGCATCGAGTACTACACGCCGACT
CCTGGGCTGCTTGAAGTCGGCGAAGAGATCGTCGAAGAAACGCTGCACTCGCACCTGCTCAAGTCCAATTGCCCGGTGAC
CGGCCAGCCCGACTGGGGCAGCGTGCTGATTCGGTATCGCGGCCCCCGGCTGGAGCGCGATGCCCTGCTGAAGTATCTGA
TCTCCTACCGGCAGCATCAGGACTTTCACGAGCACTGCGTCGAGCATCTGTTCGTGGATCTCATGGCGCGGGCCAGGCCG
GAGCGGCTGCTGGTCATGGCGCGCTACGTGAGGCGCGGCGGGCTGGATATCAGCCCCTGGCGGGGTACGCCCGGCGAGCG
GCCGCCCACGCCACTCCGGCTGGCGCGTCAGTGA

Upstream 100 bases:

>100_bases
CCGGTCGGCGGAGCGCTGGTGGCCATCGCGATCTTCATCGTCGTGTTCTTCGCGGTGGGGCTGTGGATGCTCGACCGCGG
CAAGGGGATCCGGAGCTGAC

Downstream 100 bases:

>100_bases
GCGTGCAAACTGACGCCATGCATTGAACACCGGCGGCTTCGGCCGCACATCATTACCCTATTCAGACGGCCAAGGAGATG
TTCATGGATGCCATGACCTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSERDKTLEHAPLGRESAYPEHYDAGLLFPIPRQANRAPLGLDDAALPFEGEDEWHAFELSWLDAKGKPIVAVARFRLPA
DSPSLIESKSWKLYLNSFNQTRFDRREAVIDTLERDLAQAAGASVAVALFGVEDDALMPRALPGECLDDLDVSIEYYTPT
PGLLEVGEEIVEETLHSHLLKSNCPVTGQPDWGSVLIRYRGPRLERDALLKYLISYRQHQDFHEHCVEHLFVDLMARARP
ERLLVMARYVRRGGLDISPWRGTPGERPPTPLRLARQ

Sequences:

>Translated_277_residues
MSERDKTLEHAPLGRESAYPEHYDAGLLFPIPRQANRAPLGLDDAALPFEGEDEWHAFELSWLDAKGKPIVAVARFRLPA
DSPSLIESKSWKLYLNSFNQTRFDRREAVIDTLERDLAQAAGASVAVALFGVEDDALMPRALPGECLDDLDVSIEYYTPT
PGLLEVGEEIVEETLHSHLLKSNCPVTGQPDWGSVLIRYRGPRLERDALLKYLISYRQHQDFHEHCVEHLFVDLMARARP
ERLLVMARYVRRGGLDISPWRGTPGERPPTPLRLARQ
>Mature_276_residues
SERDKTLEHAPLGRESAYPEHYDAGLLFPIPRQANRAPLGLDDAALPFEGEDEWHAFELSWLDAKGKPIVAVARFRLPAD
SPSLIESKSWKLYLNSFNQTRFDRREAVIDTLERDLAQAAGASVAVALFGVEDDALMPRALPGECLDDLDVSIEYYTPTP
GLLEVGEEIVEETLHSHLLKSNCPVTGQPDWGSVLIRYRGPRLERDALLKYLISYRQHQDFHEHCVEHLFVDLMARARPE
RLLVMARYVRRGGLDISPWRGTPGERPPTPLRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=276, Percent_Identity=51.8115942028985, Blast_Score=278, Evalue=2e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_CHRSD (Q1QXV0)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_573407.1
- ProteinModelPortal:   Q1QXV0
- SMR:   Q1QXV0
- STRING:   Q1QXV0
- GeneID:   4029142
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_1353
- NMPDR:   fig|290398.4.peg.1343
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   CSAL290398:CSAL_1353-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 31386; Mature: 31255

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERDKTLEHAPLGRESAYPEHYDAGLLFPIPRQANRAPLGLDDAALPFEGEDEWHAFEL
CCCCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
SWLDAKGKPIVAVARFRLPADSPSLIESKSWKLYLNSFNQTRFDRREAVIDTLERDLAQA
EEECCCCCCEEEEEEEECCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AGASVAVALFGVEDDALMPRALPGECLDDLDVSIEYYTPTPGLLEVGEEIVEETLHSHLL
CCCEEEEEEEECCCCCCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KSNCPVTGQPDWGSVLIRYRGPRLERDALLKYLISYRQHQDFHEHCVEHLFVDLMARARP
HCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCH
ERLLVMARYVRRGGLDISPWRGTPGERPPTPLRLARQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SERDKTLEHAPLGRESAYPEHYDAGLLFPIPRQANRAPLGLDDAALPFEGEDEWHAFEL
CCCCCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEE
SWLDAKGKPIVAVARFRLPADSPSLIESKSWKLYLNSFNQTRFDRREAVIDTLERDLAQA
EEECCCCCCEEEEEEEECCCCCCHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
AGASVAVALFGVEDDALMPRALPGECLDDLDVSIEYYTPTPGLLEVGEEIVEETLHSHLL
CCCEEEEEEEECCCCCCCCCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KSNCPVTGQPDWGSVLIRYRGPRLERDALLKYLISYRQHQDFHEHCVEHLFVDLMARARP
HCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCH
ERLLVMARYVRRGGLDISPWRGTPGERPPTPLRLARQ
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA